Abstract:
:Non-invasive detection of prostate cancer or metastases still remains a challenge in the field of molecular imaging. In our recent work of screening arginine- or lysine-rich peptides for intracellular delivery of a therapeutic agent into prostate cancer cells, an arginine-rich cell permeable peptide (NH(2)GR(11)) was found with an unexpectedly preferential uptake in prostate cancer cell lines. The goal of this work was to develop this peptide as a positron emission tomography (PET) imaging probe for specific detection of distant prostate cancer metastases. The optimal length of arginine-rich peptides was evaluated by the cell uptake efficiency of three fluorescein isothiocyanate (FITC)-tagged oligoarginines (NHGR(9), NHGR(11), and NHGR(13)) in four human prostate cell lines (LNCaP, PZ-HPV-7, DU145, and PC3). Of the three oligoarginines, NH(2)GR(11) showed the highest cell uptake and internalization efficiency with its subcellular localization in cytosol. The biodistribution of FITC-NHGR(9), FITC-NHGR(11), and FITC-NHGR(13) performed in control nude mice displayed the unique preferential accumulation of FITC-NHGR(11) in the prostate tissue. Further in vivo evaluation of FITC-NHGR(11) in PC3 tumor-bearing nude mice revealed elevated uptake of this peptide in tumors as compared to other organs. In vivo pharmacokinetics evaluated with (64)Cu-labeled NH(2)GR(11) showed that the peptide was rapidly cleared from the blood (t(1/2) = 10.7 min) and its elimination half-life was 17.2 h. The PET imaging specificity of (64)Cu-labled NH(2)GR(11) was demonstrated for the detection of prostate cancer in a comparative imaging experiment using two different human cancer xenograft models.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Hao G,Zhou J,Guo Y,Long MA,Anthony T,Stanfield J,Hsieh JT,Sun Xdoi
10.1007/s00726-010-0515-5subject
Has Abstractpub_date
2011-11-01 00:00:00pages
1093-101issue
5eissn
0939-4451issn
1438-2199journal_volume
41pub_type
杂志文章相关文献
AMINO ACIDS文献大全abstract::Amino acid networks (AANs) are undirected networks consisting of amino acid residues and their interactions in three-dimensional protein structures. The analysis of AANs provides novel insight into protein science, and several common amino acid network properties have revealed diverse classes of proteins. In this revi...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-014-1710-6
更新日期:2014-06-01 00:00:00
abstract::This study aimed to evaluate the effect of taurine (tau) supplementation to low fishmeal (FM) diets on growth performance, oxidative status, and immune response of European seabass juveniles. Four isoproteic (46% crude protein) and isolipidic (19% crude lipid) diets were formulated to contain either 25 or 12.5% FM and...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02769-4
更新日期:2019-09-01 00:00:00
abstract::Glutamate dehydrogenase (GDH) plays a crucial role in amino acid deamination and has been used as an inductor of nutrients metabolism. In this study, we cloned and analyzed the GDH cDNAs in diploids (red crucian carp), triploids and tetraploids and characterized their expression profiles upon dietary treatments. Resul...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1313-z
更新日期:2012-12-01 00:00:00
abstract::Knowledge of the subcellular location of a protein provides valuable information about its function, possible interaction with other proteins and drug targetability, among other things. The experimental determination of a protein's location in the cell is expensive, time consuming and open to human error. Fast and acc...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-013-1491-3
更新日期:2013-08-01 00:00:00
abstract::Minerals more readily adsorb amino acids with charged R groups than uncharged R groups, so that the incorporation of amino acids with charged R groups into peptides would be more frequent than for amino acids with uncharged R groups. However, 74% of the amino acids in the proteins of modern organisms contain uncharged...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-004-0106-4
更新日期:2004-08-01 00:00:00
abstract::Endoplasmic reticulum (ER) stress, implicated in various neurodegenerative processes, increases the level of intracellular Ca(2+) and leads to activation of calpain, a Ca(2+)-dependent cysteine protease. We have shown previously that S-allyl-L-cysteine (SAC) in aged garlic extracts significantly protects cultured rat ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-013-1628-4
更新日期:2014-02-01 00:00:00
abstract::Vigabatrin is a GABA derivative (gamma-vinyl GABA) which inhibits irreversibly the enzyme activity of GABA transaminase and thus increased indirectly brain GABA concentrations. We have used body temperature assay to examine the effects of Vigabatrin on thermoregulation in intact rats. In order to understand the mechan...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0754-5
更新日期:2011-05-01 00:00:00
abstract::The tailor made amino acid β-N-methyl-amino-L-alanine (BMAA) is a neurotoxin produced by cyanobacteria. It has been associated with certain forms of progressive neurodegenerative disease, including sporadic Amyotrophic Lateral Sclerosis and Alzheimer's disease. Some different reports of BMAA in cyanobacterial blooms f...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1057-1
更新日期:2012-06-01 00:00:00
abstract::Knowing the key features of the structure and the biochemistry of proteins is crucial to improving enzymes of industrial interest like β-fructofuranosidase. Gene sacA from Bacillus licheniformis ATCC 14580 codifies a sucrose-6-phosphate hydrolase, a β-fructofuranosidase (E.C. 3.1.2.26, protein BlsacA), which has no cr...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-02690-2
更新日期:2019-04-01 00:00:00
abstract::The G protein-coupled receptor kinase (GRK) family consists of seven cytosolic serine/threonine (Ser/Thr) protein kinases, and among them, GRK2 is involved in the regulation of an enormous range of both G protein-coupled receptors (GPCRs) and non-GPCR substrates that participate in or regulate many critical cellular p...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-020-02864-x
更新日期:2020-07-01 00:00:00
abstract::The role of pH-dependent ionic structures of L-amino acids in catalysis has been investigated for the two-component Mannich reactions between dimethyl malonate (DMM)/ethyl acetoacetate (EAA) and imines. As catalysts, L-amino acids performed well, even better than corresponding base catalysts and provided the β-amino c...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02798-z
更新日期:2019-11-01 00:00:00
abstract::The successful prediction of protein-folding rates based on the sequence-predicted secondary structure suggests that the folding rates might be predicted from sequence alone. To pursue this question, we directly predict the folding rates from amino acid sequences, which do not require any information on secondary or t...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1189-3
更新日期:2012-08-01 00:00:00
abstract::Cell-penetrating peptides, a group of short peptides, can traverse cell membranes to enter cells and thus facilitate the uptake of various molecular cargoes. Thus, they have the potential to become powerful drug delivery systems. The correct identification of peptides as cell-penetrating or non-cell-penetrating would ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-015-1974-5
更新日期:2015-07-01 00:00:00
abstract::The nature of the products arising from a 10 days, sterile incubation at 37°C and pH 7.2 of a 1:1 mixture of N-α-(p-tosyl)-lysine-methylesterhydrochloride and anhydrous D-glucose was investigated by fast atom bombardment mass spectrometry and(1)H and(13)C nuclear magnetic resonance spectroscopies. Differently to the r...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806957
更新日期:1993-10-01 00:00:00
abstract::Granulocyte-colony stimulating factor stimulates production and antibacterial function of neutrophiles. Therapy using the recombinant protein drug represents a major step forward in oncology. The protein has not been, however, completely sequenced at the protein level and this formed the rationale of the current study...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0312-1
更新日期:2010-04-01 00:00:00
abstract::Beyond nigrostriatal dopaminergic system, the noradrenergic locus coeruleus (LC/NE) neurons are also degenerated in patients with Parkinson's disease (PD), the second most common neurodegenerative disorder. We previously reported that microglia-mediated neuroinflammation contributes to LC/NE neurodegeneration. The pur...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-2547-1
更新日期:2018-05-01 00:00:00
abstract::N-[(Z)-N-Benzoyl- or N-boc-(2-fluorophenyl)dehydroalanyl]-(R)- or (S)-phenyl-alanines 1,2,5 and 6 were hydrogenated in the presence of chiral and achiral rhodium complexes. The optical induction is compared to the results obtained using the corresponding esters as substrates. ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s007260200018
更新日期:2002-06-01 00:00:00
abstract::Tryptophan is important both for protein synthesis and as a precursor of niacin, serotonin and other metabolites. Tryptophan is an unusual amino acid because of the complexity of its metabolism, the variety and importance of its metabolites, the number and diversity of the diseases it is involved in, and because of it...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806091
更新日期:1996-03-01 00:00:00
abstract::Nitrogen fixation of the nodule of soybean is highly sensitive to oxygen deficiency such as provoked by waterlogging of the root system. This study aimed to evaluate the effects of flooding on N metabolism in nodules of soybean. Flooding resulted in a marked decrease of asparagine (the most abundant amino acid) and a ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-016-2179-2
更新日期:2016-05-01 00:00:00
abstract::This paper analyses the presence of and changes in free amino acids and biogenic amines in red and white muscle of bigeye tuna during storage in controlled atmospheres with 2 gas mixes containing different concentrations of CO(2) and O(2). Levels of amines were generally higher in white than in red muscle, with the ex...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-003-0054-4
更新日期:2004-03-01 00:00:00
abstract::Taurine chloramine (TauCl), a product of neutrophil myeloperoxidase - halide system, formed by a reaction of taurine with HOCl, is known as an anti-microbial and anti-inflammatory long-lived oxidant. We previously reported that TauCl inhibits in vitro the production of proinflammatory cytokines (IL-6, IL-8) by RA syno...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-002-0207-x
更新日期:2002-01-01 00:00:00
abstract::Numerous indolyl amino acids and their derivatives inhibited arginase activity. The inhibition was found to be non-competitive, - at least partly - allosteric, and independent on manganese ions in the active site, and it cannot be explained by the dissociation of arginase homotrimers. Indole alone is weakly inhibitory...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-006-0470-3
更新日期:2008-02-01 00:00:00
abstract::BE-3-3-3-3 (1,15-(ethylamino)4,8,12-triazapentadecane) is a bis(ethyl)polyamine analogue under investigation as a therapeutic agent for breast cancer. Since estradiol (E(2)) is a critical regulatory molecule in the growth of breast cancer, we examined the effect of BE-3-3-3-3 on estrogen receptor α (ERα) positive MCF-...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1005-0
更新日期:2012-02-01 00:00:00
abstract::Astrocytes are glial cells in the central nervous system (CNS) that play key roles in brain physiology, controlling processes, such as neurogenesis, brain energy metabolism and synaptic transmission. Recently, immune functions have also been demonstrated in astrocytes, influencing neuronal survival in the course of ne...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0691-3
更新日期:2011-02-01 00:00:00
abstract::N-Acetyl-AA(amino acid)-NHtBu derivatives of all 20 naturally occurring amino acids have been synthesized. Syntheses were performed via solution-phase methodology with yields that allow for access to gram quantities of substrates, in most cases. Syntheses include the coupling of a hindered amine, tert-butylamine, with...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0279-y
更新日期:2010-03-01 00:00:00
abstract::The second mitochondria-derived activator of caspase (Smac/DIABLO) is a pro-apoptotic protein that released from mitochondria into the cytosol when cells undergo apoptosis. Smac promotes caspase activation by binding the inhibitors of apoptosis proteins (IAP), particularly XIAP and eliminating their inhibitory activit...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-2637-0
更新日期:2018-11-01 00:00:00
abstract::Methionine (Met) is an essential amino acid for all organisms. In plants, Met also functions as a precursor of plant hormones, polyamines, and defense metabolites. The regulatory mechanism of Met biosynthesis is highly complex and, despite its great importance, remains unclear. To investigate how accumulation of Met i...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0562-y
更新日期:2010-10-01 00:00:00
abstract::Cupiennin 1a, a cytolytic peptide isolated from the venom of the spider Cupiennius salei, exhibits broad membranolytic activity towards bacteria, trypanosomes, and plasmodia, as well as human blood and cancer cells. In analysing the cytolytic activity of synthesised all-D: - and all-L: -cupiennin 1a towards pro- and e...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0471-0
更新日期:2011-01-01 00:00:00
abstract::Antimicrobial peptides (AMPs) constitute a diverse class of naturally occurring or synthetic antimicrobial molecules that have potential for use in the treatment of drug-resistant infections. Several undesirable properties of AMPs, however, may ultimately hinder their development as antimicrobial agents. Thus, new syn...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1761-8
更新日期:2014-09-01 00:00:00
abstract::The study was designed to investigate the effects of NMDA receptor agonist on the behavioral activity in rats with experimental hyperammonemia. The experiments were performed on adult male Wistar rats. Experimental hyperammonemia was induced by intraperitoneal injections of tioacetamide (TAA, 200 mg/kg) for three cons...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-004-0122-4
更新日期:2005-02-01 00:00:00