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PCR multiplex for detection of Salmonella Enteritidis, Typhi and Typhimurium and occurrence in poultry meat.

Abstract：

:The occurrence of foodborne diseases is increasing throughout the world. Bacteria of the genus Salmonella are responsible for food poisoning and, in some cases, may be fatal. The aim of this study was to adapt the multiplex PCR technique (mPCR) on the rapid and direct identification of the presence of Salmonella sp. as well as serotypes Enteritidis, Typhi and Typhimurium in poultry carcasses (n=127) and viscera (n=73). The implementation of the standard technique using positive controls was successfully adapted. The results of Salmonella sp. detection in refrigerated viscera showed that the mPCR was able to detect Salmonella genus in 2.74% of these samples. Traditional microbiological analysis also identified the same positive samples for Salmonella sp. but was not able to differentiate the serotype. The serotype Enteritidis was detected by mPCR in 1.37% of the samples. Our conclusion was that the mPCR was able to detect the presence of these bacteria in a short period of time and enabled the identification of serotype Enteritidis in one of the samples found positive for Salmonella sp.

journal_name

Int J Food Microbiol

journal_title

International journal of food microbiology

authors

de Freitas CG,Santana AP,da Silva PH,Gonçalves VS,Barros Mde A,Torres FA,Murata LS,Perecmanis S

doi

10.1016/j.ijfoodmicro.2010.02.007

subject

Has Abstract

pub_date

2010-04-30 00:00:00

pages

15-22

issue

1-2

eissn

0168-1605

issn

1879-3460

pii

S0168-1605(10)00088-7

journal_volume

139

pub_type

杂志文章

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