Abstract:
:Apocrine metaplasia (APM) is a common finding in the breast of postmenopausal women and is seen in a broad spectrum of lesions ranging from microscopic cysts to invasive apocrine carcinoma. Apocrine metaplasia within sclerosing adenosis is known as apocrine adenosis (AA) and is considered a benign lesion of the breast. Apocrine metaplasia and AA have been the subject of many studies; however, little is known about the dynamics of cell turnover in these lesions. Recent studies have shown that some forms of AA may show altered degree of proliferation along with altered expression of bcl-2 and bax proteins. In the current study, we investigate further aspects of apoptosis to help understand the mechanisms of cell turnover in AA and APM. To investigate cell turnover in APM and AA, immunohistochemistry was used to study the expression of the apoptotic markers Bak, Mcl-1, Bcl-x, and Bcl-x(L) in 45 cases of APM (13 cases of nonpapillary APM, 21 cases of simple papillary APM, and 11 cases of complex papillary APM). Also, 34 cases of AA (23 cases of non-atypical AA [NAA] and 11 cases of atypical AA [AAA]) were included in the study. The expression of hTERT and the proliferation marker Ki-67 were also determined. The TdT-mediated dUTP nick-end labeling (TUNEL) technique was used to study the apoptotic status in 28 cases of APM (12 cases nonpapillary APM and 16 cases of papillary APM including simple and complex forms) and 22 cases of AA (15 cases of NAA and 7 cases of AAA). The results showed that all cases studied by immunohistochemistry were positive for the expression of Bak, Mcl-1, Bcl-x, and Bcl-x(L) showing a pattern of staining similar to that seen in the normal breast epithelium. There was no relation between hTERT positivity and the degree of proliferation in any of the lesions studied. The TUNEL results revealed an apoptotic index (AI) of 0.4% and 0.2% in the papillary and nonpapillary groups of APM, respectively. There was no statistical significance between the AI of these 2 groups and that of the normal breast epithelium (0.3%). The average Ki-67 index in the nonpapillary group was 0.7%, whereas in the papillary group, a value of 4% was recorded. In the cases of AA, an AI of 0.4% and 0.3% in NAA and AAA, respectively, was seen. There was no statistical significance between the AI of these 2 groups and that of the normal breast epithelium (0.3%). The Ki-67 index was 5.2% and 6.6% in the NAA and AAA, respectively. The current results show that apoptosis is not a common event in APM and AA even in the presence of increased proliferation, which may render some of these lesions more susceptible to oncogenic changes. Further studies are needed to study other apoptotic pathways that may be involved in cell turnover in these lesions.
journal_name
Ann Diagn Patholjournal_title
Annals of diagnostic pathologyauthors
Elayat G,Selim AG,Wells CAdoi
10.1016/j.anndiagpath.2009.05.001subject
Has Abstractpub_date
2010-02-01 00:00:00pages
1-7issue
1eissn
1092-9134issn
1532-8198pii
S1092-9134(09)00055-0journal_volume
14pub_type
杂志文章abstract::Recently, renal angiomyoadenomatous tumor (RAT) has been identified. However, there are no descriptions about clear cell renal cell carcinoma (RCC) with focal RAT-like features. A 33-year-old Japanese man was found to have a tumor in the left kidney. Macroscopically, the tumor extended into the perinephric fat tissue,...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2010.03.003
更新日期:2011-06-01 00:00:00
abstract:INTRODUCTION:Hydatid disease is an endemic parasitic infection caused by Echinococcus granulosus mostly seen in the Mediterranean countries. The most affected organ is the liver, however hydatidosis can be found anywhere in the human body. METHODS:The records of patients who were diagnosed with hydatid disease in our ...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2017.01.011
更新日期:2017-08-01 00:00:00
abstract::Gastrointestinal stromal tumors are a heterogeneous group with a wide spectrum of histologic features. We describe the first case of 61-year-old woman who presented gastrointestinal stromal tumors of the ampulla of Vater with osteoclast-like giant cells surrounding osteoid-like material and aneurismal bone cyst-like a...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2012.08.003
更新日期:2013-08-01 00:00:00
abstract::The purpose of the current study was to examine the use of thin-layer cytologic (TLC) preparation compared to conventional cytologic preparation (CCP) in the normal endometrium (proliferative, secretory, atrophic) and endometrial glandular and stromal breakdown (EGBD). During a 6-month period, we compiled 158 cases by...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2007.05.005
更新日期:2008-04-01 00:00:00
abstract:OBJECTIVES:Proteins p27 and c-Myc are both key players in the cell cycle. While p27, a tumor suppressor, inhibits progression from G1 to S phase, c-Myc, a proto-oncogene, plays a key role in cell cycle regulation and apoptosis. The objective of our study was to determine the association between expression of c-Myc and ...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2018.03.013
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:Anal cytology (AC) is accepted as a practical screening modality for anal cancer. However, studies suggest that AC and anal biopsy dysplasia correlation is less robust than in cervicovaginal specimens. The current study goals were to look at our institutional experience in a subset of ACs and correlate with ...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2020.151670
更新日期:2020-11-20 00:00:00
abstract::Malignant vascular neoplasms such as epithelioid hemangioendothelioma (EHE) and angiosarcoma (AS) can arise within the liver. The aim of this study was to study the expression of keratins CK7, AE1/AE3 and OSCAR in primary hepatic EHE and AS. 9 cases of hepatic EHE and 13 cases of hepatic AS were stained with ERG, CK7,...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2020.151589
更新日期:2020-12-01 00:00:00
abstract::Malacoplakia is a benign and uncommon inflammatory response that develops most frequently in the genitourinary tract but has been reported at other sites. We report the case of 67-year-old man who presented with a lesion at the base of the tongue 7 months after chemoradiation for biopsy-proven invasive squamous cell c...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章,评审
doi:10.1016/j.anndiagpath.2011.02.001
更新日期:2012-06-01 00:00:00
abstract::We report a case of synovial chondromatosis of the tibiofibular joint in a 25-year-old woman that was diagnosed by fine-needle aspiration (FNA). The patient presented with pain in the left knee and a mass in the popliteal fossa. Synovial chondromatosis usually presents with joint symptoms and is often associated with ...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/s1092-9134(00)90015-7
更新日期:2000-04-01 00:00:00
abstract::Historically, the diagnosis of Hirschsprung disease was made by evaluating multiple hematoxylin and eosin-stained slides and performing acetylcholinesterase histochemical staining. Recently, calretinin immunohistochemical staining has been reported and found to be superior to acetylcholinesterase staining in the confi...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2011.02.010
更新日期:2011-10-01 00:00:00
abstract::Uterine smooth muscle tumors are usually spindle cell lesions, but a minority is composed of epithelioid cells. Foci of clear cells can be found in these latter tumors. Recently, it has been shown that some of these tumors can be positive for HMB45, and some authors have advocated calling these lesions perivascular ep...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1053/j.anndiagpath.2004.10.011
更新日期:2005-02-01 00:00:00
abstract::Balloon cell nevi and balloon cell changes in nevi have rarely been reported in the literature. We describe a compound nevus showing focal balloon cell changes in a 20-year-old man. The melanocytic nature of the balloon cells is confirmed by an immunohistochemical study. The findings are compared with those in balloon...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2007.01.006
更新日期:2008-10-01 00:00:00
abstract::Three cases of cutaneous pseudosarcomatous polyp, a lesion recently described in the dermatopathology literature, are reported here. These benign proliferations display dramatic cytologic pleomorphism, but despite their disquieting morphological features, they have behaved in a benign fashion to date. All 3 lesions in...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2007.05.004
更新日期:2008-12-01 00:00:00
abstract::We describe 2 adult women (72 and 54 years), 1 with a low-grade noninvasive papillary urothelial carcinoma of the renal pelvis, who 14 years later developed a papillary carcinoma in 1 thyroid lobe and a medullary carcinoma in the contralateral lobe. Both neoplasms were similar in size and appeared symmetrical. Despite...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2014.08.003
更新日期:2014-10-01 00:00:00
abstract::Parotid masses remain challenging secondary to the great diversity of primary tumors that may arise in the salivary glands and propensity for regional and even distant metastases to occur in this region. Meningioma must also be considered in the differential diagnosis of parotid masses, whether from direct extension, ...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2011.08.006
更新日期:2012-04-01 00:00:00
abstract::We report the case of a rare cardiac lesion, mesothelial/monocytic incidental cardiac excrescences, and also provide a review of the literature. Diagnosis of this entity was based on both its unique morphologic features and imunohistochemical stains. Cytokeratin positivity confirmed the epithelial component, mesotheli...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章,评审
doi:10.1016/s1092-9134(00)90009-1
更新日期:2000-02-01 00:00:00
abstract::Medullary thyroid carcinoma (MTC) is an uncommon thyroid cancer comprising 5% to 8% of thyroid neoplasms. In contrast to common thyroid tumors, this tumor originates from the calcitonin-producing C cells. Regional metastases to cervical lymph nodes occur early in the disease, whereas distant metastasis occurs late. Co...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2006.08.004
更新日期:2008-02-01 00:00:00
abstract::Clear cell papillary renal cell carcinoma (ccpRCC) is a recently recognized subtype of renal cell carcinoma. In this study, we investigated the clinicopathological and immunohistochemical features in a group of 26 cases of ccpRCC, with a special emphasis on the expression of vitamin D receptor (VDR). The mean age of p...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2018.06.007
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:There is contradictory evidence in literature with respect to diagnosis and management of follicular lesions of the thyroid gland. From surgical pathology stand point, pathologists require submission and processing of entire capsule for microscopic evaluation. This can be extremely challenging especially in ...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2018.12.007
更新日期:2019-04-01 00:00:00
abstract::Pancreatic schwannomas are a rare benign mesenchymal neoplasm of the pancreas. They are thought to arise from the epineurium of either autonomic sympathetic or parasympathetic fibers, which course through the pancreas with the vagus nerve. Large pancreatic schwannomas often undergo cystic change, which causes them to ...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2006.12.017
更新日期:2008-08-01 00:00:00
abstract::We reviewed breast tissue from 19 female postpubertal and three prepubertal patients with cystic fibrosis to determine the histopathologic changes present. Autopsy material was available from 19 patients who died from complications of cystic fibrosis. Two patients had lumpectomies for breast carcinoma and an additiona...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/s1092-9134(98)80019-1
更新日期:1998-10-01 00:00:00
abstract::Radiographic identification of an abnormal lesion in the esophagus routinely occurs during workup of patients with symptomatic dysphagia. Leiomyoma is the most common benign finding; however, plexiform leiomyoma, a distinctive but rare variant, follows an unusual pattern of growth which can be a challenging surgical r...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2011.04.006
更新日期:2011-10-01 00:00:00
abstract::The ventricular assist device (VAD) is a mechanical pump that has been shown to be an effective modality of cardiac support in patients with heart failure refractory to pharmacologic intervention and who are awaiting cardiac allograft transplantation. Neuropathologic findings in these patients have not been well descr...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1053/adpa.2001.23019
更新日期:2001-04-01 00:00:00
abstract::A total of sixty samples from human breast, uterus, liver, skin and abdominal fat were fixed for 8; 24 and 48 hours at a room temperature of 20 to 22°C with neutral buffered formalin (NBF) with volume to tissue ratios of 1:1; 2:1; 5:1 and 10:1 and manually processed with isopropyl alcohol and mineral oil mixtures. All...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2011.12.003
更新日期:2012-06-01 00:00:00
abstract::Folate receptor alpha (FRα) is a membrane-bound protein with a high affinity for folate, which is necessary for the biosynthesis of amino acids and nucleotide bases. It has been shown to be a potential prognostic and therapeutic target, primarily in lung and ovarian cancer, as well as in breast cancer. The aim of this...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2020.151596
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND:Negative immunoregulatory checkpoints impede effective immune responses to tumor and reduce the action of anticancer agents. One such example is programmed death marker-1 (PD-1), an inhibitory signaling receptor expressed on activated and regulatory T-cells. PD-1 expression was reported in a few reports, but...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2017.02.003
更新日期:2017-06-01 00:00:00
abstract::Brain in the middle ear or nasal cavity: heterotopia or encephalocele? The answer to this question is greatly influenced by clinical information. Sometimes, however, this information is insufficient and the pathologist's opinion may influence patient management. It seems that most pathologists tend to get the answer w...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章,评审
doi:10.1053/j.anndiagpath.2004.04.009
更新日期:2004-08-01 00:00:00
abstract::Cases of cerebral neuroblastoma or supratentorial primitive neuroectodermal tumor with malignant gliomatous components are relatively uncommon. Less frequent is the combination of these 2 elements with a mesenchymal component. This is a case report of a lipomatous supratentorial primitive neuroectodermal tumor with gl...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2007.04.009
更新日期:2009-02-01 00:00:00
abstract::Diffuse large B-cell lymphoma (DLBCL) is the most common type of lymphoma worldwide. The current World Health Organization classification includes several subtypes based on a combination of clinical, immunohistochemical, and genetic differences. Other aggressive variants of B-cell lymphomas, including Burkitt lymphoma...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2016.07.008
更新日期:2016-12-01 00:00:00
abstract::Hypereosinophilia (HE) is defined as persistently elevated absolute eosinophil count (AEC) ≥ 1.5 × 109/L, which can be due to a variety of underlying causes. In this study, we investigated the prevalence and spectrum of T-cell lymphoproliferative disorders in 124 consecutive patients with HE by flow cytometric immunop...
journal_title:Annals of diagnostic pathology
pub_type: 杂志文章
doi:10.1016/j.anndiagpath.2019.151412
更新日期:2020-02-01 00:00:00