Abstract：
:Tumorigenesis of glioblastoma multiforme (GBM), the most aggressive primary intracranial neoplasm, is associated with aberrant PI3K/AKT/mTOR signaling. Inhibitors of mTOR, such as rapamycin (RAPA) or its analogs, have provided limited benefit. Here, we aim to decipher the signaling pathways involved in RAPA resistance. We found that RAPA induced a time-dependent activation of MAPK (pERK1/2) and MEK1/2. Inhibition of upstream kinase MEK1/2 by U0126 partially suppressed RAPA-induced ERK1/2 activation. Small interfering RNA suppression of mTOR resulted in higher pERK1/2 levels and pre-treatment with RAPA potentiated PDGF-induced activation of ERK1/2. Furthermore, nuclear localization of pERK1/2 was evident following RAPA, which was MEK1/2-dependent. Cell proliferation was significantly suppressed by combined MEK1/2 and mTOR inhibition compared to mTOR inhibition alone. These results demonstrate activation of a mitogenic pathway involving a feedback mechanism between mTOR and PI3K/ERK1/2 and support the basis for combined inhibitors in GBM treatment.
journal_name
Cancer Genomics Proteomicsjournal_title
Cancer genomics & proteomicsauthors
Albert L,Karsy M,Murali R,Jhanwar-Uniyal Msubject
Has Abstractpub_date
2009-09-01 00:00:00pages
255-61issue
5eissn
1109-6535issn
1790-6245pii
6/5/255journal_volume
6pub_type
杂志文章abstract：AIM:To identify and study targets of microRNA biomarkers of glioblastoma survival across events (death and recurrence) and phases (life expectancy or post-diagnostic) using functional and network analyses. MATERIALS AND METHODS:microRNAs associated with glioblastoma survival within and across race, gender, recurrence,...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
abstract：BACKGROUND/AIM:Epithelioid osteoblastoma is a rare benign tumor of the bone. Its pathogenesis is unknown and little is known regarding its genetic features. MATERIALS AND METHODS:Cytogenetic, RNA sequencing, reverse transcription polymerase chain reaction (RT-PCR), genomic PCR, and Sanger sequencing analyses were perf...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20141
更新日期：2019-09-01 00:00:00
abstract：:Epidemiological studies have established a causal relationship between cigarette smoking and respiratory tract cancer. The knowledge about the interaction of tobacco constituents with cellular systems is, however, still incomplete. Therefore, we analyzed 36 factors with known or assumed relevance and found that 8 prot...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
abstract：:Despite the approval of several molecular therapies in the last years, breast cancer-associated death ranks as the second highest in women. This is due to metastatic disease, which represents a challenge for treatment. A better understanding of the molecular mechanisms of metastasis is, therefore, of paramount importa...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：10.21873/cgp.20062
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Oncogenic infection by HPV, eventually leads to cervical carcinogenesis, associated by deregulation of specific pathways and protein expression at the intracellular and secretome level. Thus, secretome analysis can elucidate the biological mechanisms contributing to cervical cancer. In the present study we s...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：10.21873/cgp.20060
更新日期：2017-11-01 00:00:00
abstract：:After the completion of sequencing for dozens of genomes, as well as the draft of human genome, a major challenge is to characterize genome-wide transcriptional regulation networks. Identification of regulatory functions for transcription factor binding sites in eukaryotes greatly enhances our understanding of the net...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Cellular signal transduction pathways and gene expression are tightly regulated to accommodate changes in response to physiological environments. In the current study, molecules were identified that are activated as a result of intracellular signaling and immediately expressed as mRNA in MCF-7 breast cancer cells shor...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2008-05-01 00:00:00
abstract：BACKGROUND:Cowden syndrome (CS) is a rare autosomal-dominant inherited disorder characterized by multiple hamartomas. While the hamartomas are benign, patients with CS have increased risk of osteosarcoma and of breast, thyroid, endometrial, soft-tissue and colonic neoplasms. Germline mutations of phosphatase and tensin...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20069
更新日期：2018-03-01 00:00:00
abstract：BACKGROUND/AIM:Dedifferentiated liposarcoma (DDLPS) is recalcitrant type of sarcoma. DDLPS has a low survival rate with high recurrence and metastasis. In the present study, we evaluated the efficacy of several drugs against doxorubicin-resistant DDLPS in a patient-derived orthotopic xenograft (PDOX) model for precisio...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20194
更新日期：2020-07-01 00:00:00
abstract：BACKGROUND:Identification and characterization of genes that are relevant to pancreatic cancer remains a priority for developing detection and diagnostic tests and identifying targets for treatment. MATERIALS AND METHODS:In order to discover relevant genes, we developed a microarray composed of 5763 pancreas and pancr...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
abstract：BACKGROUND/AIM:The chromosome translocation t(14;21)(q11;q22) was reported in four pediatric T-cell lymphoblastic leukemias and was shown to activate the OLIG2 gene. MATERIALS AND METHODS:A pediatric T-cell lymphoblastic lymphoma was investigated using G-banding chromosome analysis, fluorescence in situ hybridization ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20166
更新日期：2020-01-01 00:00:00
abstract：BACKGROUND/AIM:In breast cancer, initiation of carcinogenesis leads to epigenetic dysregulation, which can lead for example to the loss of the heterochromatin skeleton SUV39H1/H3K9me3/HP1 or the supposed secondary skeleton TIP60/P400/H4K12ac/BRD (2/4), which allows the maintenance of chromatin integrity and plasticity....
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20223
更新日期：2020-11-01 00:00:00
abstract：BACKGROUND:Genetic differences between pulmonary typical carcinoids (TCs) and atypical carcinoids (ATCs) still remain controversial and no genetic marker is available for the evaluation of the malignant potential in pulmonary carcinoids. MATERIALS AND METHODS:Five TCs and 3 ATCs were analyzed concurrently by comparati...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：BACKGROUND/AIM:Pul-down assay is a popular in vitro method for identification of physical interactors of selected proteins. Here, for the first time, we compared three conventional variants of pull-down assay with the streptavidin-modified surface plasmon resonance (SPR) chips for the detection of PDZ and LIM domain pr...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20098
更新日期：2018-09-01 00:00:00
abstract：BACKGROUND/AIM:In a previous study we analyzed poliomyelitis incidence in US states, before the introduction of the polio vaccine. We noted an inverse correlation between polio incidence in US states, between 1937 and 1951, and colorectal cancer incidence (2005-2009). To further elucidate the role that poliovirus could...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20112
更新日期：2019-01-01 00:00:00
abstract：:Breast cancer in men (MBC) is an uncommon malignancy and accounts for only 1% of all diagnosed breast cancers. By using genomic and transcriptomic approaches, researchers have been able to expand our insight into the genetic basis of breast cancer, by providing new biomarkers. We currently know that gene analysis by i...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2016-05-01 00:00:00
abstract：BACKGROUND/AIM:Prostate cancer is the most common malignancy in US males. African American men have higher incidence and mortality rates than European Americans. Five single nucleotide polymorphisms are associated with PCa. We hypothesized haplotypes inferred from these SNPs are also associated with PCa. PATIENTS AND ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20076
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND:Breast cancer metastasis is associated with the motility and invasiveness of breast cancer cells. In a previous study we reported the motility enhancement effect of keratinocyte growth factor (KGF) on breast cancer cells. This study established and characterized the influence of KGF on breast cancer cell mot...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2004-07-01 00:00:00
abstract：BACKGROUND/AIM:The outcome of patients with advanced hepatocellular carcinoma (HCC) remains poor and therapeutic options, including sorafenib, the first anti-cancer drug proved to prolong survival in patients with advanced HCC, are limited. However, no clinically useful predictive biomarker for sorafenib has been repor...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20159
更新日期：2019-11-01 00:00:00
abstract：:Previous studies have suggested that galectin-3 expression was markedly elevated in papillary thyroid carcinoma compared to other thyroid diseases. In order to better understand this protein, galectin-3 from papillary thyroid carcinoma was partially purified by affinity chromatography on lactose-agarose. Proteins elut...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2008-03-01 00:00:00
abstract：BACKGROUND:O-GlcNAcylation is a unique intracellular protein modification; however, few extracellular O-GlcNAc-modified proteins have been discovered. We have previously demonstrated that many cellular proteins were aberrant in O-GlcNAcylation in breast cancer tissues. In the present study, therefore, we investigated w...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
abstract：:The perception of long non-coding RNAs as chunk RNA and transcriptional noise has been steadily replaced by their role as validated targets for a diverse set of physiological processes in the past few years. However, for the vast majority of lncRNAs their precise mode of action and physiological function remain to be ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：10.21873/cgp.20027
更新日期：2017-05-01 00:00:00
abstract：:The expression profiles of the erythropoietin producing hepatocellular carcinoma (Eph) receptor family of tyrosine kinases have been previously shown to provide molecular signatures of normal breast cells, breast tumor cells and invasive breast carcinoma cells. In particular, the expression of EphB6 receptor is lost i...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：:Although the statistical probability of therapeutic success is known for larger groups of cancer patients, the clinical response to chemotherapy of the individual patient remains uncertain. It would be of great value to know whether or not an individual tumor responds to the proposed therapy. The concept of sensitivit...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2004-03-01 00:00:00
abstract：:Breast cancer metastasis accounts for the majority of deaths from breast cancer. Detection of breast cancer metastasis at the earliest stage is important for the management and prediction of breast cancer progression. Emerging techniques using the analysis of circulating tumor cells show promising results in predictin...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2012-09-01 00:00:00
abstract：:The concept of individualized cancer chemotherapy emerged three decades ago from the observation that a small fraction of cells in primary tumors can form colonies in soft agar similar to stem cells of the hematopoietic system. In a series of retrospective and prospective clinical studies, clonogenic tumor growth and ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2007-05-01 00:00:00
abstract：BACKGROUND:The transcription factor SOX2 controls gene expression during development and has roles in both neurogenesis and gliogenesis. MATERIALS AND METHODS:The current study utilized immunohistochemistry, immunofluorescence, molecular genetics (gene copy number analysis) and Western blotting to examine SOX2 express...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2011-05-01 00:00:00
abstract：BACKGROUND:Salivary gland cancer is a common type of head and neck cancer characterized by occasional deep invasion and lung metastasis. The precise role of sex steroid hormones in salivary gland cancer is unclear. To address this issue, we investigated whether the estrogen axis modulates salivary adenocarcinoma (SAC) ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2016-07-01 00:00:00
abstract：BACKGROUND/AIM:Pseudomyogenic hemangioendothelioma is a rare endothelial tumor. Previous genetic investigations have shown that the tumors carry either a SERPINE1-FOSB or an ACTB-FOSB fusion gene. The aim of the study was to identify FOSB fusions linked with pseudomyogenic hemangioendothelioma. MATERIALS AND METHODS:R...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20134
更新日期：2019-07-01 00:00:00
abstract：:Testicular germ cell tumors (GCT) are the most frequent malignancy in young adults and arise from intratubular germ cell neoplasia undetermined (IGCNU, also referred to as carcinoma in situ, CIS). To determine the transcriptional programs involved in the transition from normal germ cells to GCT, and to further elucida...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
