Abstract:
:Gammadelta T cells are a prominent granulated cell population in the ruminant pregnant uterus and both their number and granule size increase dramatically during pregnancy. Anchor-RT-PCR was used to assess TCRdelta gene usage by gammadelta T cells from the uterine epithelium of pregnant sheep. The TCRdelta genes obtained exhibited distinct combinatorial and junctional diversity and only two out of nine V-D-J rearrangements sequenced were identical. Furthermore, two of the Vdelta elements used are also expressed in peripheral blood, indicating that gammadeltaTCR use in sheep epithelia is neither restricted nor site-specific, similar to humans but in contrast to findings in mice. Protein analysis of purified, granulated uterine gammadelta T cells revealed the presence of large amounts of the antimicrobial peptide, granulysin. The results of the present study indicate that ovine uterine gammadeltaTCR(+) intraepithelial lymphocytes have the potential to recognise diverse antigens and may have a role in protecting the uterus from infection during pregnancy and parturition. A similar protective role for gammadelta T cells may exist in the human decidua parietalis.
journal_name
J Reprod Immunoljournal_title
Journal of reproductive immunologyauthors
Fox A,Maddox JF,de Veer MJ,Meeusen ENdoi
10.1016/j.jri.2009.10.003subject
Has Abstractpub_date
2010-01-01 00:00:00pages
52-6issue
1eissn
0165-0378issn
1872-7603pii
S0165-0378(09)00496-3journal_volume
84pub_type
杂志文章abstract::The majority of anti-sperm monoclonal antibodies which were shown to inhibit in vitro fertilization in our laboratory react with antigens in the acrosomal region of spermatozoa. To elucidate the mechanism of human and/or mouse fertilization inhibition, the effects of these antibodies on mouse sperm-zona binding and mo...
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journal_title:Journal of reproductive immunology
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journal_title:Journal of reproductive immunology
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journal_title:Journal of reproductive immunology
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journal_title:Journal of reproductive immunology
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journal_title:Journal of reproductive immunology
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journal_title:Journal of reproductive immunology
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journal_title:Journal of reproductive immunology
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(85)90036-1
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(98)00020-5
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journal_title:Journal of reproductive immunology
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pub_type: 杂志文章,评审
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更新日期:1998-07-01 00:00:00
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journal_title:Journal of reproductive immunology
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journal_title:Journal of reproductive immunology
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章
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更新日期:2018-11-01 00:00:00
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章
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更新日期:1983-11-01 00:00:00