GammadeltaTCR+ cells of the pregnant ovine uterus express variable T cell receptors and contain granulysin.

Abstract:

:Gammadelta T cells are a prominent granulated cell population in the ruminant pregnant uterus and both their number and granule size increase dramatically during pregnancy. Anchor-RT-PCR was used to assess TCRdelta gene usage by gammadelta T cells from the uterine epithelium of pregnant sheep. The TCRdelta genes obtained exhibited distinct combinatorial and junctional diversity and only two out of nine V-D-J rearrangements sequenced were identical. Furthermore, two of the Vdelta elements used are also expressed in peripheral blood, indicating that gammadeltaTCR use in sheep epithelia is neither restricted nor site-specific, similar to humans but in contrast to findings in mice. Protein analysis of purified, granulated uterine gammadelta T cells revealed the presence of large amounts of the antimicrobial peptide, granulysin. The results of the present study indicate that ovine uterine gammadeltaTCR(+) intraepithelial lymphocytes have the potential to recognise diverse antigens and may have a role in protecting the uterus from infection during pregnancy and parturition. A similar protective role for gammadelta T cells may exist in the human decidua parietalis.

journal_name

J Reprod Immunol

authors

Fox A,Maddox JF,de Veer MJ,Meeusen EN

doi

10.1016/j.jri.2009.10.003

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

52-6

issue

1

eissn

0165-0378

issn

1872-7603

pii

S0165-0378(09)00496-3

journal_volume

84

pub_type

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