Abstract：
PURPOSE:Polymethylmethacrylate (PMMA) segments are normally used in additive surgery to treat both corneal ectasia post laser-assisted in situ keratomileusis and keratoconus. The aim of this work was to develop an experimental animal model to induce wound healing in the deep stroma in corneas of hens. METHODS:PMMA segments were implanted in the right eyes of 40 adult hens without suture in the wound incision. Animals were randomized for 5 time points to histopathology study (1, 3, 15, 30, and 90 days) being clinically evaluated during the experiment. RESULTS:Thirty-four eyes (85%) had a successful clinical outcome and intraoperative mistakes occurred in 6 eyes (15%), including anterior chamber perforation resulting in abscess (1), excess of lamellar dissection with segment migration (3), and peripheral incisions evolving with neovascularization (2). At 24 hours, all the epithelial injuries were completed in integrity, but a persistent stromal incision, with a fish mouth form, was observed until day 15. Corneal edema disappeared at the fifth day. Haze, keratocyte cell death, keratocyte proliferation, myofibroblast differentiation, and new matrix production were observed in length around the segment. CONCLUSIONS:Wound healing was induced in the deep corneal stroma by means of PMMA segment implantation in a new animal model developed in hens.
journal_name
Eur J Ophthalmoljournal_title
European journal of ophthalmologyauthors
Merayo-Lloves J,Blanco-Mezquita T,Ibares-Frias L,Fabiani L,Alvarez-Barcia A,Martinez-Garcia Cdoi
10.1177/112067211002000108subject
Has Abstractpub_date
2010-01-01 00:00:00pages
62-70issue
1eissn
1120-6721issn
1724-6016pii
2B199728-40A1-476E-965F-8014F192990Ejournal_volume
20pub_type
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