Abstract:
:A method has been developed for the study of somatostatin (SS) binding to dissociated cells from rat cerebral cortex. Binding of [125I] [Tyr11]SS to cells obtained by mechanical dissociation of rat cerebral cortex was dependent on time and temperature, saturable, reversible and highly specific. Under conditions of equilibrium, i.e., 60 min at 25 degrees C, native SS inhibited tracer binding in a dose-dependent manner. The Scatchard analysis of binding data was linear and yielded a dissociation constant of 0.60 +/- 0.08 nM with a maximal binding capacity of 160 +/- 16 fmol/mg protein. The binding of [125I] [Tyr11]SS was specific as shown in experiments on tracer displacement by the native peptides, SS analogues, and unrelated peptides.
journal_name
Peptidesjournal_title
Peptidesauthors
Colas B,Prieto JC,Arilla Edoi
10.1016/0196-9781(90)90138-usubject
Has Abstractpub_date
1990-11-01 00:00:00pages
1109-12issue
6eissn
0196-9781issn
1873-5169pii
0196-9781(90)90138-Ujournal_volume
11pub_type
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