Biphasic kinetics of a methanotrophic community is a combination of growth and increased activity per cell.

Abstract:

:Because methane-oxidizing bacteria (MOB) are the only biological sink for the greenhouse gas methane, knowledge of the functioning of these bacteria in various ecosystems is needed to understand the dynamics observed in global methane emission. The activity of MOB is commonly assessed by methane oxidation assays. The resulting methane depletion curves often follow a biphasic pattern of initial and induced methane oxidation activity, often interpreted as representing the in situ active and total MOB community, respectively. The application of quantitative-PCR on soil incubations, which were stopped before, at and after the transition point in the methane-depletion curve, demonstrated that both pmoA-mRNA was produced as well as substantial cell growth took place already in the initial phase. In addition, type Ia and II MOB displayed markedly different behaviour, which can be interpreted as ecologically different strategies. For the correct interpretation of methane oxidation assays, the use of small time windows is recommended to calculate methane oxidation activities to avoid substantial cell growth.

journal_name

FEMS Microbiol Ecol

authors

Steenbergh AK,Meima MM,Kamst M,Bodelier PL

doi

10.1111/j.1574-6941.2009.00782.x

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

12-22

issue

1

eissn

0168-6496

issn

1574-6941

pii

FEM782

journal_volume

71

pub_type

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