Abstract：
:A protein of approximately 70-kDa was identified as a candidate Na+/Mg2+ exchanger in rumen epithelial cells (REC). Melastatin-related Transient Receptor Potential 7 (TRPM7) and Magnesium Transporter 1 (MagT1) transcripts and, from them, encoded proteins were also detected. The regulation of these Mg transport pathways by extracellular [Mg] changes was the main focus of this study. Therefore, a 24-h pre-incubation of ovine REC in control (1.2 mM), low (0.12 mM)-Mg, and high (5 mM)-Mg medium was performed. Na+/Mg2+ exchangers, TRPM7 and MagT1 abundance and activity were investigated by Western blot analysis, flow cytometry, immunocytochemistry and fluorescence spectroscopic measurements of [Mg2+]i changes. Inhibitors were employed to differentiate Na+/Mg2+ exchanger-mediated (imipramine) and channel-mediated (cobalt(III)hexaammine, nitrendipine) Mg transport. Basal [Mg2+]i (0.40 +/- 0.02 mM) was not influenced by pre-incubation in low- or high-Mg medium. However, compared with control REC (4.1 +/- 0.7 microM/min), such cells showed reduced (2.8 +/- 0.6 microM/min) or elevated (6.4 +/- 0.9 microM/min) Mg extrusion rates that correlated with a decreased (25%) and increased (38%) expression of the putative Na+/Mg2+ exchanger protein, respectively. Low- and high-Mg pre-incubated REC were both characterized by an increased (30-40%) influx capacity. In low-Mg REC, the latter resulted mainly from a strong activation of the TRPM7-related transport component. The data thus clearly demonstrate the intrinsic regulation of REC transmembrane Mg transport.
journal_name
Magnes Resjournal_title
Magnesium researchauthors
Schweigel M,Kuzinski J,Deiner C,Kolisek Msubject
Has Abstractpub_date
2009-09-01 00:00:00pages
133-50issue
3eissn
0953-1424issn
1952-4021journal_volume
22pub_type
杂志文章abstract：:Adequate magnesium (Mg) levels play a vital role in membrane excitability, cell contractility and metabolism, being a key nutrient for sustaining appropriate muscular contraction and performance levels in athletes. Phase angle (PhA), assessed by bioimpedance analysis (BIA), has been reported to be positively associate...
journal_title：Magnesium research
pub_type： 杂志文章
doi：10.1684/mrh.2015.0389
更新日期：2015-07-01 00:00:00
abstract：INTRODUCTION:The present study investigated associations between environmental exposure to magnesium (Mg) and the levels of oxidative stress parameters and selected cytokines, and the antioxidant defense system in the seminal plasma of fertile males. MATERIALS AND METHODS:The study population consisted of 57 healthy, ...
journal_title：Magnesium research
pub_type： 杂志文章
doi：10.1684/mrh.2015.0377
更新日期：2015-01-01 00:00:00
abstract：:Audiogenic seizures triggered by an acoustic stimulus of determined frequency and amplitude have been described in many laboratory animals in many circumstances including magnesium deficiency. This model, recently validated, was used, in DBA/2 mice, to study the preventive neuroprotective effect of 6 wavelengths of th...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：:In the present study, we investigated the protective effects of magnesium sulfate (MgSO4) against carbon tetrachloride (CCl4)-induced liver damage in rats. MgSO4 (0.001, 0.01, 0.05 and 0.1 Mg(2+) g/kg b.wt.) was administered intragastrically for 28 consecutive days to male, CCl4-treated rats. The hepatoprotective acti...
journal_title：Magnesium research
pub_type： 杂志文章
doi：10.1684/mrh.2014.0350
更新日期：2013-10-01 00:00:00
abstract：:Although magnesium (Mg) contents are different between breast milk and formula, few studies have investigated the blood Mg level in breast fed or formula fed preterm infants. We examined the influence of feeding type on serum Mg and whole blood ionized Mg (iMg) levels in preterm infants soon after birth. We included 1...
journal_title：Magnesium research
pub_type： 杂志文章
doi：10.1684/mrh.2019.0444
更新日期：2018-11-01 00:00:00
abstract：:Competitive divalent (magnesium and calcium) or monovalent (potassium, lithium and sodium) ion exchange and its influence on a membrane potential formation was studied at biological ligands (BL) such as adenosine triphosphate (ATP), asparagine (Asn) and glutamine (Gln) sites. The sites are dispersed electrochemically ...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：2009-03-01 00:00:00
abstract：:The use of fluorescent indicators to quantify intracellular magnesium ([Mg(2+)]i) requires accurate determination of the dissociation constant (K(D)). Ideally, the K(D) should be determined intracellularly; however, in practice this is difficult to achieve. An alternative method is to measure the K(D) in solutions whi...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:Traumatic brain injury (TBI) confers a major burden to Western society and effective treatments are urgently required to improve the long-term deficits that inflict TBI survivors. Depletion of intracellular Mg(2+) is a well-known phenomenon occurring after TBI and is associated with poor neurological outcome. However,...
journal_title：Magnesium research
pub_type： 杂志文章,评审
doi：10.1684/mrh.2009.0189
更新日期：2009-12-01 00:00:00
abstract：:In therapy it is known that the combination of vitamin B6 and magnesium is beneficial in the treatment of several forms of primary magnesium deficiency. The purpose of the present study was to investigate the effect of complex magnesium supplementation containing mineral bishofit solution (MgCl2 x 6H2O) and pyridoxine...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：2002-12-01 00:00:00
abstract：:Increased white blood cell counts or leukocytosis have been observed primarily in young rats fed diets extremely low in Mg (< 60 ppm) and high in phosphorus (0.5 % P). We investigated the influence that acute and moderate Mg deficiencies have on blood leukocytes at high and low dietary phosphorus levels. For four week...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：:Magnesium (Mg) deficiency strongly affects muscle performance. In judo, many athletes often undergo impressive weight changes associated with severe dehydration. Common practices used by athletes to achieve a target weight can lead to Mg deficit. This study aimed to understand the impact of Mg changes on strength from...
journal_title：Magnesium research
pub_type： 杂志文章
doi：10.1684/mrh.2010.0217
更新日期：2010-09-01 00:00:00
abstract：:We studied the voltage dependence of the inhibitory effects of the Ca2+ antagonist Mg2+, compared to nifedipine, on the transsarcolemmal Ca2+ uptake into primary monolayers of smooth muscle cells obtained from pig coronary arteries. The cellular Ca incorporation was analyzed by liquid scintillation. Depolarization was...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：1997-06-01 00:00:00
abstract：:The present study was performed to show the molecular identity of functionally characterized Mg transport pathways in rumen epithelial cells (REC) and to investigate the effects of extracellular [Mg] changes on their expression and activity. By using RT- PCR, Western blot, flow cytometry and immunocytochemistry, TRPM7...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：2008-06-01 00:00:00
abstract：:There is an important functional link between magnesium and calciotropic hormones. PTH stimulates magnesium reabsorption in the renal tubule, absorption in the gut and release of the ion from bone. Magnesium on the other hand is essential for the normal function of the parathyroid glands, metabolism of vitamin D and a...
journal_title：Magnesium research
pub_type： 杂志文章,评审
doi：
更新日期：1995-03-01 00:00:00
abstract：:The purpose of the research work was to study the influence of alpha-zearalenol on magnesium concentration in cellular subfractions of certain tissues of female rats and their progeny. Pregnant female rats received alpha-zearalenol at the doses of: 0.05, 0.5 or 1 mg x kg(-1) b.w. After delivery, brain, liver and kidne...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：2003-06-01 00:00:00
abstract：:Serum and mononuclear cell magnesium content were determined in a cross-sectional study performed in four groups of hypertensive patients on chronic treatment with atenolol (n = 11), enalapril (n = 10), thiazide diuretics (n = 12), or enalapril + thiazides (n = 11). Our study shows that in patients treated with the th...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：1990-09-01 00:00:00
abstract：:A tight coupling between ionic currents, intracellular Ca2+ homeostasis, cytosolic [ADP] and deltaG of ATP hydrolysis underlies the regulation of cardiac cell function. As more experimental detail on the biochemistry and biophysics of these complex processes and their interactions accumulates, the intuitive interpreta...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：2008-03-01 00:00:00
abstract：:Cerebral vasospasm and delayed cerebral ischemia remain an unsolved problem in patients with aneurysmal subarachnoid hemorrhage (SAH). In theory, high-dose magnesium sulfate (MgSO(4)) therapy offers vascular and neuroprotective benefits and is therefore currently under evaluation. The intensity of the inflammatory res...
journal_title：Magnesium research
pub_type： 临床试验,杂志文章
doi：
更新日期：2012-07-01 00:00:00
abstract：:Catechol-O-methyltransferase (COMT) is a magnesium-dependent, catecholamine-metabolizing enzyme, whose impaired activity has been positively associated with cardiovascular diseases, particularly hypertension. Consumption of some natural mineral-rich waters has been shown to exert protective effects on cardiovascular r...
journal_title：Magnesium research
pub_type： 杂志文章
doi：10.1684/mrh.2014.0369
更新日期：2014-07-01 00:00:00
abstract：:In clinical practice diabetes mellitus is the most significant cause of hypomagnesemia and Mg depletion. The obese Zucker rat approaches non-insulin-dependent type II diabetes; lean Zucker rats being suitable controls. Using this disease model the influence of dietary Mg deficiency was studied: animals received a diet...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:Hypomagnesaemia has been reported among patients undergoing hemodialysis (HD). Recently, a possible correlation between serum magnesium (Mg) and C-reactive protein (CRP) has been stressed. This correlation has been attributed to cytokine production and oxidative stress processes. In this study we aimed to determine th...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：2008-09-01 00:00:00
abstract：:Intracellular total Mg2+ and free Mg2+ are compartmentalized between cell organelles and within the cytosol. Different values of [Mg2+]i in the cytosol of the same cell type were measured by various investigators. A main reason for the differences is the uncertainty of the dissociation constants used for the Mg furapt...
journal_title：Magnesium research
pub_type： 杂志文章,评审
doi：
更新日期：2006-12-01 00:00:00
abstract：UNLABELLED:Comprehensive analytical review of 44 human studies in 43 publications of oral Magnesium (Mg) therapy for hypertension (HT) shows Mg supplements may enhance the blood-pressure (BP) lowering effect of anti-hypertensive medications (medications) in Stage 1 HT subjects. 9 studies conducted on subjects treated w...
journal_title：Magnesium research
pub_type： 杂志文章
doi：10.1684/mrh.2010.0198
更新日期：2010-03-01 00:00:00
abstract：:Mg2+ is known to influence conductance and gating properties of a multitude of ion channels and is thus able to modulate synaptic transmission. Therefore, a tight regulation of the intracellular free Mg2+ concentration ([Mg2+]i) in neurones and glial cells is crucial for maintaining the functions of central nervous sy...
journal_title：Magnesium research
pub_type： 杂志文章,评审
doi：
更新日期：2000-06-01 00:00:00
abstract：:More and more studies are accumulating about COVID-19. Some aspects of the pathogenesis of the disease recall events occurring in Mg deficiency, such as a drop of T cells, increased plasma concentration of inflammatory cytokines, and endothelial dysfunction. We hypothesize that a low Mg status, which is rather common,...
journal_title：Magnesium research
pub_type： 杂志文章
doi：10.1684/mrh.2020.0465
更新日期：2020-05-01 00:00:00
abstract：:In this study, the effects of oral magnesium supplementation were evaluated on plasma, erythrocyte, and urinary cation levels. The study was carried out with an open, cross-over and randomized design. Healthy subjects received two sachets per day of magnesium (366 mg Mg2+/d) for two 28 d cycles, separated by a 3-week ...
journal_title：Magnesium research
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1993-06-01 00:00:00
abstract：:We measured free intralymphocyte magnesium (Mgi) in 10 hypertensive subjects (HS) before and after treatment with atenolol (A) and in 16 normotensive control subjects (NC). We also carried out in vitro studies on human lymphocytes to test the effect of catecholamines and beta-antagonists on Mgi. Mgi in HS was not stat...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:A common use for high doses of oral magnesium salts is to produce a laxative effect to treat constipation. In the intestinal lumen the poorly absorbable magnesium ions (and other ions such as sulphate) exert an osmotic effect and cause water to be retained in the intestinal lumen. This increases the fluidity of the in...
journal_title：Magnesium research
pub_type： 杂志文章,评审
doi：
更新日期：1996-06-01 00:00:00
abstract：:Lactating rats fed a 0.055% Mg diet transferred 5.5 +/- SEM 0.3 mg per day of Mg into milk but remained in a slightly positive Mg balance by increasing both food consumption and fractional intestinal Mg absorption. Urinary Mg excretion increased continually during the course of lactation to values double those of non-...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:46 children, aged 2-6 years, with decreased magnesium concentrations in hair, were studied. Magnesium supplementation consisted of Asmag preparation for 3 months and multivitamin Multi-tabs preparation (containing magnesium, but without calcium) for the following 4 months. Control studies were performed again after 7 ...
journal_title：Magnesium research
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
