Abstract:
PURPOSE:Malassezia yeasts are globally distributed agents of pityriasis versicolor and are implicated in the pathogenesis of seborrhoeic and atopic dermatitis. The aim of this study is to identify the Malassezia species obtained from pityriasis versicolor patients, using morphological, biochemical, physiological as well as Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods. MATERIALS AND METHODS:The identification of Malassezia species is performed according to microscopic features and physiological characteristics, including catalase reaction and Tween assimilation tests. The DNA is extracted from cultured Malassezia using the glass bead, phenol-chloroform method. The internal transcribed spacer 1(ITS1) region is amplified and there is restricted digestion of the PCR products with two enzymes Cfo I and Bst F5I. RESULTS:The most commonly isolated species is M. globosa (47.6%). RFLP analysis of the PCR products of the ITS1 region is in complete agreement with those from the DNA sequences of the internal transcribed spacer (ITS) 1 region and the biochemical tests. CONCLUSION:Based on the findings of this study, it can be concluded that PCR-RFLP is a relatively simple and quick method, completely comparable to the routine methods used for Malassezia identification.
journal_name
Indian J Med Microbioljournal_title
Indian journal of medical microbiologyauthors
Shokohi T,Afshar P,Barzgar Adoi
10.4103/0255-0857.55445subject
Has Abstractpub_date
2009-10-01 00:00:00pages
321-4issue
4eissn
0255-0857issn
1998-3646pii
IndianJMedMicrobiol_2009_27_4_321_55445journal_volume
27pub_type
杂志文章abstract:PURPOSE:To report a fatal case of disseminated trichosporonosis caused by Trichosporon asahii in a patient with acute myeloblastic leukemia (AML) and to present an update on systemic trichosporonosis with special reference to India. METHODS:The diagnosis was based on repeated demonstration of budding yeast cells and a...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Post-operative Nocardia endophthalmitis has an aggressive course and poor visual prognosis. It often masquerades as severe post-operative uveitis or toxic anterior segment syndrome due to the absence of vitreous involvement resulting in a delay in diagnosis. The poor prognosis in Nocardia endophthalmitis is due to sev...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.99506
更新日期:2012-07-01 00:00:00
abstract::Dengue has become endemic in India with outbreaks caused by all the four serotypes occurring almost every year. Dengue cases have been increasing alarmingly in Assam also. This study aimed to identify the dengue serotypes circulating in Assam. Clinically suspected dengue fever cases were included in the study. Viral R...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_17_121
更新日期:2018-07-01 00:00:00
abstract::Ocular infection with microsporidia has been documented in both immunocompetent and immunocompromised patients. Sources and mode of human infection with microsporidia have been difficult to ascertain although exposure to water may be an important risk factor. Of four genera that have been reported in human disease, on...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.90189
更新日期:2011-10-01 00:00:00
abstract::Probiotics are friendly live microorganisms (in most cases, bacteria) that are similar to beneficial micro-organisms found in the human gut, whenever consumed, have potential to confer benefit to the health of consumers by maintaining, or improving their intestinal microbial flora and are available to consumers mainly...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.4103/0255-0857.150868
更新日期:2015-02-01 00:00:00
abstract:PURPOSE:The non-sporing anaerobes cause a wide spectrum of infections. They are difficult to culture and their identification is tedious and time-consuming. Rapid identification of anaerobes is highly desirable. Towards this end, the potential of nuclear magnetic resonance (NMR) spectroscopy for providing a fingerprint...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.37334
更新日期:2007-10-01 00:00:00
abstract:PURPOSE:The intestinal flagellate Giardia lamblia includes many genetically distinct assemblages, of which assemblage A and B, predominantly infect humans. Nitroimidazoles derivatives (metronidazole and tinidazole) and nitazoxanide are some of the therapeutic agents for treatment of giardiasis. Nevertheless, some indiv...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.142236
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:Humoral immune responses in cytomegalovirus (CMV) are not studied well. Pre-transplant CMV immunoglobulin G (IgG) antibody levels (Pre-Tx IgG) could influence the occurrence of post-transplant CMV infections. OBJECTIVE:Correlation between pre-Tx IgG and post-Tx risk of acquiring CMV infection was investigat...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_17_201
更新日期:2017-10-01 00:00:00
abstract::Invasive fungal infections (IFIs) are an important cause of morbidity and mortality in paediatric leukaemias. Antifungal combinations to treat these patients are being explored. Fourteen children with leukaemias and IFIs were treated with a combination of antifungal agents at our centre. The first antifungal was ampho...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_18_157
更新日期:2019-01-01 00:00:00
abstract::A 14 year old girl from a coastal district of Odisha presented with a six month history of asymptomatic brownish patches on the palm of the both hands. Epidermal scrape from these patches showed brown septate hyphae with occasional yeast like cells. Hortaea wernekii was isolated from the fungal culture. A diagnosis of...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.124336
更新日期:2014-01-01 00:00:00
abstract::Histopathological examination of tissue biopsies for the identification of infectious organisms is a very important diagnostic tool. Conventional culture confirmation of tissue biopsies often fail to identify any pathogen as, first of all, invariably most of the tissue samples that are collected and sent for culture i...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.4103/0255-0857.49423
更新日期:2009-04-01 00:00:00
abstract::Thermophilic Campylobacter are commonly associated with poultry as commensals of the avian gut and are the causative agent responsible for human Campylobacteriosis. This study aimed to establish the prevalence of Campylobacter spp. from environmental sources that have previously been implicated as sources of horizonta...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.142259
更新日期:2014-10-01 00:00:00
abstract::Neonatal listeriosis is widely reported, but this is the first case reported in Serbia. A newborn developed respiratory distress syndrome 2 hours after delivery and was admitted to the neonatal intensive care unit. Initial empirical therapy was inappropriate. Consequently, on the second day, the patient developed meni...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.115229
更新日期:2013-04-01 00:00:00
abstract:PURPOSE:Opportunistic viral infections cause increased morbidity and mortality among human immunodeficiency virus (HIV) infected individuals, especially those who are not on antiretroviral treatment. Early diagnosis of these opportunistic viruses will be able to reduce the risk of disease progression with appropriate i...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.53202
更新日期:2009-07-01 00:00:00
abstract:Background and Objectives:Human papillomavirus (HPV) is the causative agent of cervical cancer, a major cause of cancer mortality in Indian women. The current study was undertaken to add information to the existing data on HPV type distribution in Indians, in an attempt to document HPV types for future vaccination prog...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_19_451
更新日期:2019-10-01 00:00:00
abstract:PURPOSE:The objective of this study is to determine the role of quantitative cultures of non-bronchoscopic samples such as blinded bronchial sampling (BBS) and endotracheal aspirates (ETA) in the management of ventilator associated pneumonia (VAP). The study also evaluates the clinical diagnosis of VAP based on the inc...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.25226
更新日期:2006-04-01 00:00:00
abstract::[This corrects the article DOI: 10.4103/0255-0857.188314.]. ...
journal_title:Indian journal of medical microbiology
pub_type: 已发布勘误
doi:10.4103/0255-0857.195384
更新日期:2016-10-01 00:00:00
abstract::An outbreak of food poisoning due to Salmonella enterica serotype Weltevreden ( S.weltevreden ) involving 34 students has been reported from a tertiary care hospital in Mangalore. The symptoms developed 8-10 hours, after consuming a non- vegetarian dish, probably fish, from an outside caterer. The identity of the orga...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.53211
更新日期:2009-07-01 00:00:00
abstract:PURPOSE:Genotyping studies like spoligotyping are valuable tools in understanding the genetic diversity and epidemiology of Mycobacterium tuberculosis. Though there are reports of spoligotyping of M. tuberculosis isolates from pulmonary specimens from different parts of India, spoligotyping of extra pulmonary tuberculo...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.154871
更新日期:2015-04-01 00:00:00
abstract:AIM:To evaluate E-test as a tool for rapid determination of drug susceptibility against the conventional LJ method focusing on reliability, expense, ease of standardization and performance of the technique in low resource settings. MATERIALS AND METHODS:A total of 74 clinical isolates (2004-2005) of Mycobacterium tube...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.58725
更新日期:2010-01-01 00:00:00
abstract::In the present report, we have analysed C.trachomatis infection and HIV positivity among patients (n-143) who attended the STD clinic at the Institute of STDs, Government General Hospital, Chennai. HIV positivity rate was significantly high among those with chlamydial infection than in those without chlamydial infecti...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.13871
更新日期:2005-01-01 00:00:00
abstract::A case of invasive multiple paranasal sinus aspergillosis with bony involvement is reported. A young immunocompetent lady presented with bilateral nasal obstruction due to polyps. Radiologically and histopathologically a fungal cause was kept a possibility, and the diagnosis of Aspegillus fumigatus was established by ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.16595
更新日期:2005-07-01 00:00:00
abstract::The term superantigen was introduced in microbiology and immunology only a decade ago. The unique feature of superantigen is that it bypasses the antigen processing mechanism and specifically binds to TCR v segment and forms a trimolecular complex along with major histocompatibility complex class II. Since its discove...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
abstract::Salmonella Typhi has rarely been associated with focal abscesses; and in literature, there is no evidence of its association with abscesses in the neck spaces. Ability of Salmonella Typhi to invade and localise in the neck spaces not only poses a diagnostic challenge but also underscores the necessity to understand th...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/0255-0857.154889
更新日期:2015-04-01 00:00:00
abstract::Injection abscess is an iatrogenic infection occurring as an isolated case or as cluster outbreak. These infections occur due to contaminated injectables or lapse in sterilisation protocol. While pathogens such as Pseudomonas, Klebsiella, E. coli, and S. aureus are the usual causative agents, unusual organisms such as...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2003-04-01 00:00:00
abstract:Purpose:The Revised National Tuberculosis Control Programme (RNTCP) is now introducing daily fixed-dose regimen instead of Directly Observed Treatment, Short Course (DOTS) regimen for treatment of drug-sensitive tuberculosis (TB) in India. It would be beneficial to understand the drawbacks, barriers and advantages of t...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_18_65
更新日期:2018-04-01 00:00:00
abstract:PURPOSE:The purpose of this study was to evaluate the discriminatory power of two DNA-based technique and a protein-based technique for the typing of nosocomial isolates of Klebsiella pneumoniae. A second objective was to determine the antimicrobial susceptibility pattern and characterise the presence of genes encoding...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:10.4103/ijmm.IJMM_16_308
更新日期:2017-07-01 00:00:00
abstract::Salmonella Typhi can be a significant cause of morbidity and mortality in pregnant females with adverse outcomes. Risk of infections increases manifold during pregnancy due to hormonal changes and immunological phenomena. S. Typhi has the ability to cross placenta (vertical transmission) resulting in miscarriage, stil...
journal_title:Indian journal of medical microbiology
pub_type:
doi:10.4103/ijmm.IJMM_16_180
更新日期:2017-04-01 00:00:00
abstract:PURPOSE:The Western Blot test is considered a gold standard test for the confirmation of an ELISA and/or rapid assay screened reactive sample in the diagnosis of HIV infection, especially in the low risk population. In this study, an indigenously developed HIV W. Blot kit (J.Mitra & Co., New Delhi, India) was compared ...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00
abstract::The aetiology of appendicitis is usually polymicrobial. We report a rare case of appendicitis caused by Streptococcus pneumoniae as the only causative organism. The case assumes significance because it occurs in the absence of any predisposing factor for invasive pneumococcal infection, it is unimicrobial, it may lead...
journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00