Molecular analysis of bacterial microbiota in the gut of the termite Reticulitermes speratus (Isoptera; Rhinotermitidae).

Abstract:

:The molecular diversity and community structure of bacteria from the gut of the termite Reticulitermes speratus were analyzed by the sequencing of near-full-length 16S rRNA genes, amplified by polymerase chain reaction. The results of the analysis of 1344 clones indicated a predominance of spirochetes in the gut. Spirochetal clones accounted for approximately half of the analyzed clones. The clones related to Bacteroides, Clostridia, and the candidate division Termite Group I each accounted for approximately 5-15% of the analyzed clones. The rest were comprised of Proteobacteria, Actinobacteria, Mycoplasma and others. Using the criterion of 97% sequence identity, the clones were sorted into 268 phylotypes, including 100 clostridial, 61 spirochetal and 31 Bacteroides-related phylotypes. More than 90% of the phylotypes were found for the first time, and some constituted monophyletic clusters with sequences recovered from the gut of other termite species.

journal_name

FEMS Microbiol Ecol

authors

Hongoh Y,Ohkuma M,Kudo T

doi

10.1016/S0168-6496(03)00026-6

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

231-42

issue

2

eissn

0168-6496

issn

1574-6941

pii

FEM231

journal_volume

44

pub_type

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