Matrix metalloproteinase-9 potentiates early brain injury after subarachnoid hemorrhage.

Abstract:

OBJECTIVE:This study investigated the role of matrix metalloproteinase-9 (MMP-9) in early brain injury after subarachnoid hemorrhage (SAH). METHOD:Sprague-Dawley male rats (n=36) weighing between 250 and 300 g were used. SAH was produced by injecting autologous arterial blood into the pre-chiasmatic cistern. MMP-9 protein expression and activity were measured by Western blot and zymogram; laminin expression and neuronal cell in hippocampus were studied by immunohistochemistry and TUNEL staining at 24 hours after SAH in the presence or absence of a selective MMP-9 inhibitor SB-3CT. RESULT:MMP-9 was activated by SAH and inhibited by SB-3CT at 24 hours after SAH (p<0.01). Laminin, the substrate of MMP-9, was decreased at 24 hours after SAH, and SB-3CT prevented laminin degradation. The number of TUNEL-positive neurons in hippocampus was increased after SAH and decreased by SB-3CT (p<0.01). In addition, brain water content and neurological functional abnormalities were attenuated by SB-3CT. CONCLUSION:MMP-9 may be involved in early brain injury through degradation of laminin and neuronal death, and inhibition of MMP-9 may be a potential direction for brain protection after SAH.

journal_name

Neurol Res

journal_title

Neurological research

authors

Guo Z,Sun X,He Z,Jiang Y,Zhang X,Zhang JH

doi

10.1179/016164109X12478302362491

subject

Has Abstract

pub_date

2010-09-01 00:00:00

pages

715-20

issue

7

eissn

0161-6412

issn

1743-1328

pii

ner2010

journal_volume

32

pub_type

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