Suppression of myostatin with vector-based RNA interference causes a double-muscle effect in transgenic zebrafish.

Abstract:

:Myostatin belongs to the transforming growth factor (TGF)-beta superfamily and is a potent negative regulator of skeletal muscle development and growth. We utilized microinjection of an antisense RNA-expressing vector to establish a hereditarily stable myostatin gene knockdown zebrafish strain with a double-muscle phenotype. Real-time PCR and immunostaining revealed that the myostatin messenger (m)RNA and protein levels in homozygous transgenic zebrafish were 33% and 26% those of the non-transgenic controls, respectively. Also, the mRNA levels of myogenic regulatory factor markers such as MyoD, myogenin, Mrf4, and Myf5 were dramatically elevated in myostatin-suppressed transgenic fish compared to the non-transgenic controls. Although there was no significant difference in body length, homozygous transgenic zebrafish were 45% heavier than non-transgenic controls. Histochemical analysis showed that the cross-sectional area of the muscle fiber of homozygous transgenic fish was twice as large as that of non-transgenic controls. This is the first model zebrafish with a hereditarily stable myostatin-suppressed genotype and a double-muscle phenotype.

authors

Lee CY,Hu SY,Gong HY,Chen MH,Lu JK,Wu JL

doi

10.1016/j.bbrc.2009.07.110

subject

Has Abstract

pub_date

2009-10-02 00:00:00

pages

766-71

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(09)01471-5

journal_volume

387

pub_type

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