Abstract：
:Malaria represents a major global health problem but despite extensive efforts, no effective vaccine is available. Various vaccine candidates have been developed that provide protection in animal models, such as a gene gun-delivered DNA vaccine encoding the circumsporozoite protein (CSP) of Plasmodium berghei. A common shortcoming of most malaria vaccines is the requirement for multiple immunizations leaving room for improvement even for established vaccine candidates such as the CSP-DNA vaccine. In this study, we explored whether regulating apoptosis in DNA vaccine transfected host cells could accelerate the onset of protective immunity and provide significant protection after a single immunization. A pro-apoptotic gene (Bax) was used as a molecular adjuvant in an attempt to mimic the immunostimulatory apoptosis triggered by viral or virus-derived vaccines, while anti-apoptotic genes such as Bcl-XL may increase the life span of transfected cells thus prolonging antigen production. Surprisingly, co-delivery of either Bax or Bcl-XL greatly reduced CSP-DNA vaccine efficacy after a single immunization. Co-delivery of Bax for three immunizations still had a detrimental effect on protective immunity, while repeated co-delivery of Bcl-XL had no negative impact. The fine characterization of humoral and cellular immune response modulated by these two molecular adjuvants revealed a previously unknown effect, i.e., a shift in the Th-profile. These results demonstrate that pro- or anti-apoptotic molecules should not be used as molecular adjuvants without careful evaluation of the resulting immune response. This finding represents yet another example that strategies to enhance vaccine efficacy developed for other model systems such as viral diseases cannot easily be applied to any vaccine.
journal_name
Vaccinejournal_title
Vaccineauthors
Bergmann-Leitner ES,Leitner WW,Duncan EH,Savranskaya T,Angov Edoi
10.1016/j.vaccine.2009.06.059subject
Has Abstractpub_date
2009-09-18 00:00:00pages
5700-8issue
41eissn
0264-410Xissn
1873-2518pii
S0264-410X(09)00922-0journal_volume
27pub_type
杂志文章相关文献
VACCINE文献大全abstract：:To study the regulation of the human immune response to hepatitis B surface antigen (HBsAg) we have carefully monitored the in vivo humoral and in vitro cellular immune responses to HBsAg in 50 subjects receiving four doses of hepatitis B vaccine according to a 0, 1, 2, 12 month vaccination scheme. Twenty-three subjec...
journal_title：Vaccine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0264-410x(94)90290-9
更新日期：1994-07-01 00:00:00
abstract：:Bacterial flagellin is known to induce potent immune response in vertebrate systems via the toll-like receptor (TLR) 5. As a result, flagellin has been studied extensively as a vaccine adjuvant. In a previous study, we examined the vaccine and adjuvant potentials of the flagellin (FliC) of the fish pathogen Edwardsiel...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2010.06.022
更新日期：2010-08-02 00:00:00
abstract：:The hypervariable region 1 (HVR1) of hepatitis C virus (HCV) may contain neutralizing epitopes. A chimpanzee in whom cross-reactive anti-HVR1 antibodies had been induced by immunization was challenged with heterologous HCV for clarifying whether cross-reactive anti-HVR1 antibodies can neutralize heterologous HCV. Acut...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(02)00271-2
更新日期：2002-08-19 00:00:00
abstract：:Delayed-type hypersensitivity (DTH) response to arthropod vector salivary proteins is associated with protection against pathogen transmission. Massive cDNA sequencing, high-throughput DNA plasmid construction and DNA immunisation were used to identify twelve DTH inducing proteins isolated from a Phlebotomus ariasi sa...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2005.07.085
更新日期：2006-01-16 00:00:00
abstract：CONTEXT:The true level of influenza vaccine efficacy is controversial and many factors may influence its estimation. OBJECTIVES:To estimate the efficacy of vaccination of children and non-elderly adults for the prevention of influenza and to explore the impact of type of vaccine, age, degree of strain matching, influe...
journal_title：Vaccine
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.vaccine.2012.10.084
更新日期：2012-12-17 00:00:00
abstract：BACKGROUND:The compliance with influenza vaccination among health-care workers (HCWs) is known to be low. A multi-nationality survey to explore the reasons for such poor compliance has not been studied in depth. MATERIALS AND METHODS:An epidemiologic survey to evaluate the compliance rates with influenza vaccination a...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2009.06.108
更新日期：2009-09-04 00:00:00
abstract：:Transmission-blocking vaccines against malaria parasites target molecules expressed by sexual stage parasites to elicit antibodies that prevent the infection of the mosquito vector. Pvs25 and Pvs28, expressed on the surface of ookinetes, are potential candidates for such a vaccine and induce antibodies that block the ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(01)00402-9
更新日期：2001-12-12 00:00:00
abstract：:In an agent-based computer model, we simulate the formation and recall of anti-virus immunological memory. Specifically we try to predict what will happen, both to the response and to memory, when the second infecting virus is partly different from the first one, and when the cross-reactivity of the two branches of th...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2008.11.109
更新日期：2009-02-05 00:00:00
abstract：:To demonstrate the potential of pH-sensitive carbonate apatite (CO₃Ap) nanoparticles as DNA vaccine carriers to enhance vaccination efficacy, we examined the humoral and cellular immune responses of C57BL/6 mice immunized with the plasmid expression vector pCI-neo encoding the full-length soluble ovalbumin (OVA) (pCI-...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2014.09.032
更新日期：2014-10-29 00:00:00
abstract：BACKGROUND:British Columbia (BC) introduced a school-based HPV vaccine program in September 2008. As part of the HPV vaccine program evaluation, we determined the type-specific HPV prevalence in a population-based sample of women presenting for routine cervical cancer screening in the province. METHODS:From June 2010 ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2012.09.085
更新日期：2013-02-04 00:00:00
abstract：:Despite the recommendation of the Dutch association of nursing home physicians (NVVA) to be immunized against influenza, vaccine uptake among HCWs in nursing homes remains unacceptably low. Therefore we conducted a cluster randomised controlled trial among 33 Dutch nursing homes to assess the effects of a systematical...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2010.05.003
更新日期：2010-07-12 00:00:00
abstract：OBJECTIVE:was to improve understanding of mechanisms contributing to healthcare personnel's (HCP) reluctance to get vaccinated against seasonal influenza. We assessed the role of several drivers: vaccination knowledge, vaccination recommendations and the role of the Internet (so-called e-health) in creating vaccination...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2011.12.021
更新日期：2012-02-01 00:00:00
abstract：:We previously reported the development of an oral vaccine for diabetes based on live attenuated Salmonella-expressing preproinsulin (PPI) as the autoantigen. When combined with host cell-expressed TGFβ, the vaccine prevented the onset of diabetes in non-obese diabetic (NOD) mice. Herein, we investigated factors that c...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2018.10.101
更新日期：2018-12-18 00:00:00
abstract：:To determine the potential for biodegradable alginate microspheres to be used as a delivery vehicle for DNA based vaccines, we constructed a plasmid, pMNe-gal-SV40, containing the bacterial beta-galactosidase (LacZ) gene under the control of the murine cytomegalovirus (MCMV) immediate-early promoter and the simian vir...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(00)00170-5
更新日期：2000-09-15 00:00:00
abstract：:The unique genetic makeup and remarkable competency of Vibrio cholerae are the key factors that help the cholera pathogen adapt rapidly to adverse environmental conditions and resist the detrimental effect of antimicrobial agents. In the last few decades, V. cholerae that causes acute watery diarrhoeal disease cholera...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2019.06.031
更新日期：2020-02-29 00:00:00
abstract：:Recombinant hepatitis B surface antigen (HBsAg) particles derived from Chinese hamster ovary (CHO) cells were stored at various conditions for 12-18 months in their naked form or adsorbed to alum (HBV vaccine). The physical, chemical and immunological parameters during storage at -20 degrees C, 4 degrees C, room tempe...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(99)00149-8
更新日期：1999-08-20 00:00:00
abstract：:CD8(+) T-cell responses provide beneficial antiviral immunity against human immunodeficiency virus 1 (HIV-1). In this study, we show that intragastric vaccination with a Salmonella HIV-1 Env DNA vaccine vector generates Env-specific CD8(+) T-cells, both in mucosal and systemic lymphoid tissue. By contrast, intramuscul...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(01)00330-9
更新日期：2001-11-12 00:00:00
abstract：:In order to perform preliminary evaluations of subunit vaccine candidates before extensive trials in large food-producing animals, an in vitro B-lymphocyte response assay, based on the principles of an ELISA, was established. The assay was developed in detail for the porcine system using antigens from the Gram-negativ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(91)90149-z
更新日期：1991-03-01 00:00:00
abstract：BACKGROUND:Native American populations experience a substantial burden of pneumococcal disease despite use of highly effective pneumococcal conjugate vaccines (PCVs). Protein-based pneumococcal vaccines may extend protection beyond the serotype-specific protection elicited by PCVs. METHODS:In this phase IIb, double-bl...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2019.09.076
更新日期：2019-12-03 00:00:00
abstract：OBJECTIVE:The purpose of this study was to assess the awareness and knowledge regarding human papillomavirus (HPV), HPV related conditions and HPV vaccine among nursing students, and to identify their attitudes towards HPV vaccination. MATERIALS AND METHODS:The study was carried out at a tertiary Women's Health Care C...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2013.01.033
更新日期：2013-04-19 00:00:00
abstract：:The reactogenicity and immunogenicity of the administration of recombinant vaccine against hepatitis B simultaneously (but at separate sites) with diphtheria/tetanus/pertussis (DTP) and oral polio vaccines were examined. Six hundred and twenty-six children (group I) were given hepatitis B vaccine at 0, 2 and 6 months ...
journal_title：Vaccine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0264-410x(95)00040-8
更新日期：1995-08-01 00:00:00
abstract：:The present study was undertaken to determine the effectiveness in including VP2 gene of the large segment genome of infectious bursal disease virus (IBDV) in the DNA vaccine for protection of chickens against infectious bursal disease (IBD). Different fragments of the large segment gene of IBDV standard challenge (ST...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(02)00473-5
更新日期：2003-01-17 00:00:00
abstract：:Monoclonal antibody 3C9 was the starting material in the definition of the epitope that led to the synthesis of the first efficient peptide vaccine against a viral disease (canine parvovirus) in the natural host (dog). In this report, we have analysed the specificity of the antibody at the single amino acid level and ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(00)00526-0
更新日期：2001-03-21 00:00:00
abstract：:Influenza virus hemagglutinin (HA) and neuraminidase (NA) proteins elicit protective antibody responses and therefore, are used as targets for vaccination, especially the HA protein. However, these proteins are subject to antigenic drift, decreasing vaccine efficacy, and few to no studies have analyzed antigenic varia...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2018.09.005
更新日期：2018-10-15 00:00:00
abstract：:This paper presents the results of a global survey that aimed to collect information on country's immunization policy development processes, particularly on the presence and function of national Immunization Technical Advisory Groups (ITAGs). Characteristics of national ITAGs are described as well as attributes of the...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2009.07.089
更新日期：2010-04-19 00:00:00
abstract：:We evaluated invasive pneumococcal disease (IPD), antimicrobial resistance and nasopharyngeal colonization before and after introduction of pneumococcal conjugate vaccine (PCV7) in Alaska Natives (AN), a population with high IPD rates. We obtained IPD rates from population-based surveillance. Colonization was determin...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2005.08.100
更新日期：2005-12-01 00:00:00
abstract：:Previous immunological studies from our laboratory have demonstrated the potential role of Toxoplasma gondii antigens SAG1 and GRA2 as vaccine candidates. To further evaluate the vaccine's effects, a series of recombinant DNA vaccines pVAX1-SAG1, pVAX1-GRA2 and pVAX1-SAG1-GRA2, termed pSAG1, pGRA2 and pSAG1-GRA2, resp...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2012.01.004
更新日期：2012-02-27 00:00:00
abstract：:In the present study the homologous and heterologous type and subtype specific cellular immune response induced by a wild type inactivated whole virus H5N1 Influenza (A/Vietnam/1203/2004) vaccine was evaluated. Two immunizations with the Vero cell derived H5N1 influenza vaccine on Day 0 and Day 21 induced significant ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2010.10.065
更新日期：2010-12-16 00:00:00
abstract：BACKGROUND:To contribute to the global demand for oral cholera vaccine (OCV), the production of Euvichol® was scaled up with elimination of thimerosal. To demonstrate the equivalence of the variations, a study was carried out in the Philippines. METHODS:Healthy male and female adults and children in Manila were random...
journal_title：Vaccine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.vaccine.2018.05.102
更新日期：2018-07-05 00:00:00
abstract：:The role of in vivo-induced ApxIV toxin of Actinobacillus pleuropneumoniae in protective immunity was evaluated in pigs by administering it alone or added to a multicomponent recombinant subunit vaccine composed of recombinant ApxI, ApxII, ApxIII toxin, and 42-kDa outer membrane protein (OMP). The pigs were immunized ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2009.07.065
更新日期：2009-09-25 00:00:00
