Abstract：
:In situ cell separation and immobilization of bacterial cells for biodesulfurization were developed by using superparamagnetic Fe(3)O(4) nanoparticles (NPs). The Fe(3)O(4) NPs were synthesized by coprecipitation followed by modification with ammonium oleate. The surface-modified NPs were monodispersed and the particle size was about 13 nm with 50.8 emu/g saturation magnetization. After adding the magnetic fluids to the culture broth, Rhodococcus erythropolis LSSE8-1 cells were immobilized by adsorption and then separated with an externally magnetic field. The maximum amount of cell mass adsorbed was about 530 g dry cell weight/g particles to LSSE8-1 cells. Analysis showed that the nanoparticles were strongly absorbed to the surface and coated the cells. Compared to free cells, the coated cells not only had the same desulfurizing activity but could also be easily separated from fermentation broth by magnetic force. Based on the adsorption isotherms and Zeta potential analysis, it was believed that oleate-modified Fe(3)O(4) NPs adsorbed bacterial cells mainly because of the nano-size effect and hydrophobic interaction.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Li YG,Gao HS,Li WL,Xing JM,Liu HZdoi
10.1016/j.biortech.2009.05.064subject
Has Abstractpub_date
2009-11-01 00:00:00pages
5092-6issue
21eissn
0960-8524issn
1873-2976pii
S0960-8524(09)00633-6journal_volume
100pub_type
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