Immunogenicity and protective efficacy of orally or intranasally administered recombinant Lactobacillus casei expressing ETEC K99.

Abstract:

:In an effort to develop a safe and effective vaccine for the prevention of enterotoxigenic Escherichia coli (ETEC) K99 infections, we have developed a surface antigen display system using pgsA (poly-gamma-glutamate synthetase A) as an anchoring matrix. The recombinant fusion proteins comprised of pgsA and fimbriae protein of ETEC K99 were stably expressed on Lactobacillus casei. Surface localization of the fusion protein was verified by immunoblotting, immunofluorescence microscopy and flow cytometry. Specific Pathogen Free (SPF) BALB/c mice orally or intranasally vaccinated with recombinant L. casei resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA against ETEC K99, as demonstrated by enzyme-linked immunosorbent assays using purified fimbriae peptides. The serum antibody isotypes elicited were predominantly IgG1 and IgG2a. Vaccinated SPF BALB/c mice were evaluated by oral challenge with standard-type ETEC C83912 after the last booster immunization. More than 80% of immunized mice survived regardless of the immune route. The antibody titers elicited following oral immunization were lower than those following intranasal immunization but the protective efficacy was in the same order of magnitude. These results indicate that mucosal immunization with recombinant L. casei expressing ETEC K99 fimbriae protein on its surface provides an effective means for eliciting a protective immune response against the ETEC K99.

journal_name

Vaccine

journal_title

Vaccine

authors

Wei CH,Liu JK,Hou XL,Yu LY,Lee JS,Kim CJ

doi

10.1016/j.vaccine.2009.05.088

subject

Has Abstract

pub_date

2010-05-28 00:00:00

pages

4113-8

issue

24

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(09)00839-1

journal_volume

28

pub_type

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