Abstract：
:Measurements with extracellular microelectrode technique showed that depolarization of motor nerve terminals in frog cutaneous pectoris muscle with high-potassium solution (40 mM K(+)) increased frequency of miniature end-plate currents. Both fast intracellular calcium chelator BAPTA-AM and slow chelator EGTA-AM equally moderated the increase in the frequency of miniature end-plate currents. Intravital fluorescent microscopy with FM 1-43 dye showed that under conditions of stimulation of neurotransmitter exocytosis and secretion with high-potassium solution, internalization of the dye into newly-formed endocytotic synaptic vesicles proceeded both in the control and in the presence of EGTA-AM. In contrast, internalization of the dye was not observed in the presence of BAPTA-AM. It was concluded that asynchronous exocytosis of synaptic vesicles goes on in the active zones enriched with Ca-channels due to activation of high-affinity Ca-site in Ca-macrodomain. Endocytosis of vesicles is probably initiated by Ca-microdomain during activation of low-affinity Ca-site in the immediate proximity to the Ca channel.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Zefirov AL,Grigor'ev PNdoi
10.1007/s10517-009-0369-6subject
Has Abstractpub_date
2008-12-01 00:00:00pages
667-70issue
6eissn
0007-4888issn
1573-8221journal_volume
146pub_type
杂志文章abstract：:Olivomycin (DNA-binding antibiotic) in nanomolar concentrations induces apoptosis of human tumor cells and inhibits p53-dependent transcription of the reporter gene (basal and induced by antitumor drugs). Olivomycin aglycon induces no cytotoxicity and does not block transcription. ...
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pub_type： 临床试验,杂志文章
doi：
更新日期：2000-10-01 00:00:00
