Rapid Aeromonas hydrophila identification by TaqMan PCR assay: comparison with a phenotypic method.

Abstract:

AIMS:Aeromonas hydrophila is recognized as a human pathogen following wound exposure or ingestion of contaminated water and food. For rapid identification of this bacterium, a TaqMan-based real-time PCR assay has been developed. METHODS AND RESULTS:Primers and probes that target specific sequences of the 16S rRNA gene and cytolytic enterotoxin gene (aerA) were combined in a duplex assay. Presence and size of PCR products were confirmed with microchannel fluidics electrophoresis analysis. After validation, using type strain CIP7614T DNA, the PCR assay was tested on 12 positive and negative controls. Twenty-one Aeromonas strains were isolated from environmental samples and were identified with biochemical tests as Aer. sobria, Aer. caviae and Aer. hydrophila. Only Aer. hydrophila strains tested positive by PCR assay. CONCLUSIONS:The PCR developed here was successfully applied for the identification of Aer. hydrophila from reference, clinical and environmental samples and showed a high discrimination between Aer. hydrophila and other Aeromonas species. SIGNIFICANCE AND IMPACT OF THE STUDY:This molecular method is convenient, rapid (2.5 h vs 24 h), specific to identify Aer. hydrophila and usable for diagnosis in medical and veterinary laboratories.

journal_name

Lett Appl Microbiol

authors

Trakhna F,Harf-Monteil C,Abdelnour A,Maaroufi A,Gadonna-Widehem P

doi

10.1111/j.1472-765X.2009.02635.x

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

186-90

issue

2

eissn

0266-8254

issn

1472-765X

pii

LAM2635

journal_volume

49

pub_type

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