Abstract:
INTRODUCTION:The emergence of methicillin-resistant Staphylococcus aureus (MRSA) has become a major epidemiological problem worldwide. METHODS:We determined the degree of association between the genotype screening results obtained by pulse-field gel electrophoresis (PFGE) and polymerase chain reaction (PCR) for 7 hypervariable DNA regions associated with the mecA gene (HVR-mecA PCR), in the epidemiological analysis of 36 MRSA strains unrelated to nosocomial outbreaks, isolated from hospitalized patients at the County Hospital of Valdivia (Chile). RESULTS:The strains were classified into 15 pulse types (A-O) and 5 genotypes (6, 14, 15, 16, and 17) by PFGE and HVR-mecA PCR, respectively. Most of the strains were grouped in pulse types D, E and I, which presented 85.7% similarity. The most common genotypes were 14 (36.1%) and 15 (33.3%). Each genotype detected by HVR-mecA PCR was distributed in more than one pulse type. The degree of association between genotypic screening by PFGE or HVR-mecA PCR was determined by calculating Cramer's V statistic and the contingency coefficient. In both cases, a value near 1 (0.84 and 0.78, respectively) was obtained, indicating a high association between these genotypic screenings. Thus these are complementary, not exclusionary techniques that can be equally applied. CONCLUSIONS:PFGE is a standardized, high-technology molecular tool with considerable discriminatory power. HVR-mecA PCR is a fast, simple, accessible tool that has lower discriminatory power; nonetheless it can serve as an alternative method for epidemiological research in MRSA strains.
journal_name
Enferm Infecc Microbiol Clinjournal_title
Enfermedades infecciosas y microbiologia clinicaauthors
Medina G,Otth C,Araya P,Hormazabal JC,Fernández J,Maldonado A,Fernández H,Otth L,Wilson Mdoi
10.1016/j.eimc.2008.08.001subject
Has Abstractpub_date
2009-04-01 00:00:00pages
213-8issue
4eissn
0213-005Xissn
1578-1852pii
S0213-005X(08)00064-5journal_volume
27pub_type
杂志文章abstract:BACKGROUND:Streptococcus agalactiae or streptococcus group B (SGB) is the main etiologic agent of early neonatal sepsis. A multicenter study was performed with the aim of determining the incidence and characteristics of this disease in our medium and contribute the design of an adequate prevention protocol. METHODS:Te...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,多中心研究
doi:
更新日期:1998-08-01 00:00:00
abstract::Mycobacterium fortuitum is a rapidly growing mycobacteria recovered from human infections such as skin, soft tissue, skeletal and pulmonary infectious diseases. We report 2 cases of M. fortuitum isolation from clinical samples from two patients placed on dialysis program. Our clinical findings were: The first, the pre...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:
更新日期:1990-05-01 00:00:00
abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is an increasingly common cause of hospital acquired pneumonia (HAP) and the second most frequently isolated pathogen from patients who die from HAP. High-risk units for MRSA colonization such as intensive care (ICU's) are the most affected. Multiple risk factors for ...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1157/13091219
更新日期:2005-12-01 00:00:00
abstract:BACKGROUND:There is little information regarding peripheral facial palsy (PFP) as a complication of varicella. We describe 2 adults who developed varicella-related PFP, in 1 case as a part of Guillain-Barré syndrome (GBS), and review all reported cases of this condition. METHODS:MEDLINE search. RESULTS:A total of 10 ...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1016/j.eimc.2009.09.009
更新日期:2010-10-01 00:00:00
abstract::The fimbriated adhesines constitute a factor of virulence of E. coli in the urinary tract infections. The presence of type 1 fimbriae and fimbriae P was evaluated in 23 E. coli strains isolated with patients with chronic bacterial prostatitis. For the diagnosis of prostatitis the Stamey test was used. The detection of...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1989-03-01 00:00:00
abstract::Staphylococcus aureus is a versatile organism with several virulent characteristics and resistance mechanisms at its disposal. It is also a significant cause of a wide range of infectious diseases in humans. S. aureus often causes life-threatening deep seated infections like bacteremia, endocarditis and pneumonia. Whi...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1157/13086552
更新日期:2006-03-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to know, through a national survey, the methods and techniques used for the diagnosis of Helicobacter pylori (Hp) in the different Clinical Microbiology Services/Laboratories in Spain, as well as antibiotic resistance data. METHODS:The survey requested information about the diagn...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2019.11.008
更新日期:2020-11-01 00:00:00
abstract:INTRODUCTION:New tools are needed for early evaluation of patients who could be infected by COVID-19 during this pandemic. M-Health (apps) could be a solution in this setting to evaluate a COVID-19 diagnosis. The aim of this study was to describe which COVID-19s apps are available in Spain. METHODS:We made a review of...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2020.08.002
更新日期:2020-09-09 00:00:00
abstract:INTRODUCTION:The aim of this study was to analyze the distribution of human papillomavirus (HPV) genotypes in cytologically abnormal cervical samples from 106 women living in a region of the north of Spain. METHODS:Cytological classification was reported according to the 2001 Bethesda System and HPV genotyping was per...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2011.10.021
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND:Study of the serologic response in two groups of patients with pulmonary tuberculosis: with and without treatment. METHODS:Antibodies (type IgG) against the A60 antigen were studied in 103 patients with pulmonary tuberculosis: 46 without specific treatment and 57 after the application of the chemotherapy. ...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract:INTRODUCTION:St. Louis encephalitis virus (SLEV) is a re-emerging arbovirus in South America, with reported cases in humans in Argentina and Brazil. This fact indicates that there is an urgent need to increase the current knowledge about this virus in order to control and prevent future cases. Exhaustive epidemiologica...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1157/13114389
更新日期:2008-01-01 00:00:00
abstract:INTRODUCTION:Primary lymphoma is the most common neoplasm of the central nervous system (CNS) in AIDS patients. METHODS:We retrospectively reviewed the clinical manifestations, neuroimaging findings, diagnostic methods used, histological characteristics, detection of Epstein-Barr virus (EBV) DNA by PCR in cerebrospina...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1016/s0213-005x(04)73105-5
更新日期:2004-06-01 00:00:00
abstract:BACKGROUND:Immigration is a recent phenomenon in Spain. Certain subgroups of the immigrant population may be vulnerable to acquiring sexually transmitted infections (STI). MATERIAL AND METHODS:Descriptive study of the seroprevalenceof certain STI (HIV, hepatitis B and syphilis) and the general characteristicsn of pers...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/s0213-005x(02)72777-8
更新日期:2002-04-01 00:00:00
abstract:BACKGROUND:The purpose of this work was to study the molecular basis of beta-lactamase production in ampicillin-resistant strains of Salmonella spp. METHODS:It was performed analytical isoelectric focusing of beta-lactamases produced by a group of 33 strains selected in basis of their resistance phenotype. Plasmid pro...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract::Rilpivirine (RPV) is a new, second-generation nonnucleoside reverse transcriptase inhibitor (NNRTI) that has been recently approved for use in the initial antiretroviral therapy (ART) of treatment-naïve HIV-infected patients, combined with two nucleoside/nucleotide reverse transcriptase inhibitors (NRTI). The approved...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1016/S0213-005X(13)70139-3
更新日期:2013-06-01 00:00:00
abstract::The relevance of mixed genital infections in our area was evaluated, and we report their clinical and epidemiological features for both viral types. We evaluated overall 54 Herpes simplex virus (HSV) isolates from 44 patients seen at the clinic of sexually transmitted diseases (STD) of the Sevilla School of Medicine d...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract:BACKGROUND:To determine variability, if exists, in interleukin-6 (IL-6) response by different cell cultures stimulated by Borrelia burgdorferi. METHODS:Five human embryonic diploid fibroblast cell lines (three of them of lung origin) and four human synovial cell cultures were used. Induction of IL-6 was made with B. b...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::The high genetic diversity of human immunodeficiency virus (HIV) poses a significant challenge to the diagnosis and microbiological characterization of HIV infection. Because of the continual emergence of new variants and the global spread of HIV groups, subtypes and recombinant forms, accurate diagnostic tools are of...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1157/13128783
更新日期:2008-11-01 00:00:00
abstract:INTRODUCTION:Streptococcus pneumoniae is a significant cause of morbidity and mortality. Children with certain conditions are at risk of developing pneumococcal disease, including invasive pneumococcal disease (IPD). The aim of this study is to estimate admission rates for IPD in children with risk conditions in Catalo...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2015.07.006
更新日期:2016-05-01 00:00:00
abstract::Although the WHO original target date for the global eradication of poliomyelitis was the year 2000 -thanks to vaccination and institutional, public and private, resources for that purpose-, in 2013 the disease remained endemic in three countries, Afghanistan, Pakistan and Nigeria, and some cases were described in fiv...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1016/j.eimc.2014.10.005
更新日期:2015-12-01 00:00:00
abstract:BACKGROUND:To analyze the production and repercussions of the scientific activity of Spanish authors in the fields of microbiology, mycology, parasitology and virology. METHODS:Documents published during the period of 1990 to 2000 compiled on the MEDLINE database >(WEBSPIRS 4.2) were retrieved using the following sear...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1157/13078799
更新日期:2005-08-01 00:00:00
abstract:BACKGROUND:Visceral leishmaniasis (VL) is an endemic parasitosis in Spain with a frequency which is increasing, specially among those patients with HIV infection. METHODS:The clinical characteristics of 36 episodes of VL in 20 patients with HIV infection diagnosed in the authors' center from January 1988 to October 19...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1995-02-01 00:00:00
abstract:INTRODUCTION:The pathophysiological changes that determine the severity of dengue are still not well known, therefore it is important to study the probable relationship with the host genetic. METHODS:We analyzed the possible association between the FcγRIIa polymorphism and clinical signs in individuals who suffered de...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2012.05.011
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVE:To describe the clinical experience with dalbavancin in the treatment of diabetic foot infection in a multidisciplinary unit of a second level hospital. METHODS:A retrospective, descriptive study was made with all patients with diabetic foot infection treated with dalbavancin in the Diabetic Foot Unit of Hos...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2020.11.013
更新日期:2021-01-12 00:00:00
abstract:BACKGROUND:It has been observed an spectacular increasing of opportunistic Candida yeast infections. Many of them are fatal, and rapid and effective identification of the infecting species contributes to start the correct treatment. Several new methods for yeast identification have become available; Microring YT is one...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract::The development of novel direct antiviral agents (DAAs) against hepatitis C virus (HCV) has represented a breakthrough in the treatment of chronic hepatitis C. Telaprevir and boceprevir are the first two protease inhibitor (PI) DAAs to be approved for combination therapy with pegylated interferon (PEG-IFN) and ribavir...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/S0213-005X(13)70113-7
更新日期:2013-02-01 00:00:00
abstract:OBJECTIVES:To prospectively study the emergence and diagnostic problems of bronchiolitis caused by influenza A virus among children durign the 1999-2000 influenza epidemic. MATERIALS AND METHODS:Negative respiratory syncitial virus (RSV) specimens (ELISA dot-blot) were seeded in the MDCK cell line for the isolation of...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/s0213-005x(01)72703-6
更新日期:2001-12-01 00:00:00
abstract:BACKGROUND:Several recent studies have shown that the combination of ddI plus TDF can produce an unexpected drop in CD4 cell counts, even after correcting the ddI dose. OBJECTIVE:Comparative study of the immunological effectiveness of various once-daily NRTI backbones (ddI plus TDF, ddI plus 3TC or TDF plus 3TC) in an...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1157/13091779
更新日期:2006-08-01 00:00:00
abstract::We analyze the experience in serologic diagnosis of Lyme's borreliosis. From a total of 551 patients studied from 1987 to 1989, we further evaluate 80 cases with erythema chronicum migrans or a clinical diagnosis of Lyme's disease and positive serological tests. The techniques used were IFI, ELISA1 (Whittaker Bioprodu...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract:OBJECTIVES:To analyse and compare the ability of procalcitonin (PCT), C-reactive protein (CRP) and leukocytes to differentiate true bacteraemia from contaminated blood cultures in patients seen in the emergency department (ED) for an episode of infectious disease. METHODS:Observational, retrospective and descriptive a...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2019.01.012
更新日期:2019-11-01 00:00:00