Two-photon imaging using adaptive phase compensated ultrashort laser pulses.

abstract：:The formation of kidney stones is a common and highly studied disease, which causes intense pain and presents a high recidivism. In order to find the causes of this problem, the characterization of the main compounds is of great importance. In this sense, the analysis of the composition and structure of the stone can ...

journal_title：Journal of biomedical optics

authors： Blanco F,López-Mesas M,Serranti S,Bonifazi G,Havel J,Valiente M

更新日期：2012-07-01 00:00:00

abstract：:We report a new two-channel fluorescence microscopy technique for surface-generated fluorescence. The realized fluorescence microscope allows high resolution imaging of aqueous samples. The core element of the instrument is a parabolic mirror objective that is used to collect the fluorescence at large surface angles a...

journal_title：Journal of biomedical optics

authors： Verdes D,Ruckstuhl T,Seeger S

更新日期：2007-05-01 00:00:00

abstract：:Photodynamic therapy (PDT) is an alternative to radical surgical resection for T1a or nonresectable carcinomas of the gastroesophageal junction. Besides the concentration of the photosensitizer, the light distribution in tissue is responsible for tumor destruction. For this reason, knowledge about the behavior of ligh...

journal_title：Journal of biomedical optics

authors： Holmer C,Lehmann KS,Wanken J,Reissfelder C,Roggan A,Mueller G,Buhr HJ,Ritz JP

更新日期：2007-01-01 00:00:00

abstract：:Dihydroartemisinin (DHA), a front-line antimalarial herbal compound, has been shown to possess promising anticancer activity with low toxicity. We have previously reported that DHA induced caspase-3-dependent apoptosis in human lung adenocarcinoma cells. However, the cellular target and molecular mechanism of DHA-indu...

journal_title：Journal of biomedical optics

authors： Lu YY,Chen TS,Wang XP,Li L

更新日期：2010-07-01 00:00:00

abstract：:Laser speckle contrast imaging is a technique used for imaging blood flow without scanning. Though several studies have attempted to combine spatial and temporal statistics of laser speckle images for reducing image noise as well as preserving acceptable spatiotemporal resolution, the statistical accuracy of these spa...

journal_title：Journal of biomedical optics

authors： Qiu J,Li P,Luo W,Wang J,Zhang H,Luo Q

更新日期：2010-01-01 00:00:00

abstract：:Pedicle screw (PS) fixation has been widely used for spine diseases. Scientists and clinicians employ several approaches to navigate PS during operation. We have demonstrated the feasibility of monitoring the reduced scattering coefficient (μ's) on the trajectory of PS using near-infrared spectroscopy (NIRS). To perfo...

journal_title：Journal of biomedical optics

authors： Liu Y,Wang Y,Qian Z,Zhao J,Cao X,Li W

更新日期：2014-01-01 00:00:00

abstract：:The ability to noninvasively image embryonic vascular anatomy in mouse models is an important requirement for characterizing the development of the normal cardiovascular system and malformations in the heart and vascular supply. Photoacoustic imaging, which can provide high resolution non invasive images of the vascul...

journal_title：Journal of biomedical optics

authors： Laufer J,Norris F,Cleary J,Zhang E,Treeby B,Cox B,Johnson P,Scambler P,Lythgoe M,Beard P

更新日期：2012-06-01 00:00:00

abstract：:We have applied wide-field digital interferometry (WFDI) to examine the morphology and dynamics of live red blood cells (RBCs) from individuals who suffer from sickle cell anemia (SCA), a genetic disorder that affects the structure and mechanical properties of RBCs. WFDI is a noncontact, label-free optical microscopy ...

journal_title：Journal of biomedical optics

authors： Shaked NT,Satterwhite LL,Telen MJ,Truskey GA,Wax A

更新日期：2011-03-01 00:00:00

abstract：:Limited cutaneous systemic sclerosis (lcSSc) was formerly known as CREST syndrome in reference to the associated clinical features: calcinosis, Raynaud's phenomenon, esophageal dysfunction, sclerodactyly, and telangiectasias. The transforming growth factor beta has been identified as a major player in the pathogenic p...

journal_title：Journal of biomedical optics

authors： Barolet D

更新日期：2014-01-01 00:00:00

abstract：:A method for the prediction of the average photon pathlength in turbid media has been developed. The method is based on spatially resolved diffuse reflectance with discrete source detector distances up to 2 mm. Light reflectance was simulated using a Monte Carlo technique with a one-layer model utilizing a wide range ...

journal_title：Journal of biomedical optics

authors： Nilsson H,Larsson M,Nilsson GE,Strömberg T

更新日期：2002-07-01 00:00:00

abstract：:Two pronounced absorption peaks in blue and red ranges of the chlorin-based photosensitizer (PS) absorption spectrum provide additional benefits in photodynamic therapy (PDT) performance. Differing optical properties of biological tissues in these ranges allow for both dual-wavelength diagnostics and PDT performance. ...

journal_title：Journal of biomedical optics

authors： Kurakina D,Khilov A,Shakhova M,Orlinskaya N,Sergeeva E,Meller A,Turchin I,Kirillin M

更新日期：2019-12-01 00:00:00

abstract：:Optical coherence tomography (OCT) is an emerging technology for high-resolution, noncontact imaging of transparent and scattering media. Polarization-sensitive optical coherence tomography (PS-OCT) is a functional extension of OCT that can image birefringent properties of a biological sample. PS-OCT was used to measu...

journal_title：Journal of biomedical optics

authors： Götzinger E,Pircher M,Sticker M,Fercher AF,Hitzenberger CK

更新日期：2004-01-01 00:00:00

abstract：:The spectroscopy of analyte-specific molecular vibrations in tissue thin sections has opened up a path toward histopathology without the need for tissue staining. However, biomedical vibrational imaging has not yet advanced from academic research to routine histopathology due to long acquisition times for the microsco...

journal_title：Journal of biomedical optics

authors： Kröger N,Egl A,Engel M,Gretz N,Haase K,Herpich I,Kränzlin B,Neudecker S,Pucci A,Schönhals A,Vogt J,Petrich W

更新日期：2014-01-01 00:00:00

abstract：:In-vivo imaging of the vascular system can provide novel insight into the dynamics of vasoconstriction and vasodilation. Fourier domain optical coherence tomography (FD-OCT) is an optical, noncontact imaging technique based on interferometry of short-coherent near-infrared light with axial resolution of less than 10 m...

journal_title：Journal of biomedical optics

authors： Meissner S,Müller G,Walther J,Morawietz H,Koch E

更新日期：2009-05-01 00:00:00

abstract：:Fast estimation of optical properties from reflectance measurements at two spatial frequencies could pave way for real-time, wide-field and quantitative mapping of vital signs of tissues. We present a machine learning-based approach for estimating optical properties in the spatial frequency domain, where a random fore...

journal_title：Journal of biomedical optics

authors： Panigrahi S,Gioux S

更新日期：2018-12-01 00:00:00

abstract：:This study aimed to evaluate the concept of using high-resolution optical coherence tomography (OCT) imaging to rapidly assess surgical specimens and determine if cancer positive margins were left behind in the surgical bed. A mouse model of breast cancer was used in this study. Surgical specimens from 30 animals were...

journal_title：Journal of biomedical optics

authors： Savastru D,Chang EW,Miclos S,Pitman MB,Patel A,Iftimia N

更新日期：2014-05-01 00:00:00

abstract：:In part I of the study, two attachment systems [O-ring; bar-clip (BC)] were used, and the system with three individualized O-rings provided the lowest stress on the implants and the support tissues. Therefore, the aim of this study was to assess the stress distribution, through the photoelastic method, on implant-reta...

journal_title：Journal of biomedical optics

authors： Pesqueira AA,Goiato MC,da Silva EV,Haddad MF,Moreno A,Zahoui A,dos Santos DM

更新日期：2014-06-01 00:00:00

abstract：:Occlusal discoloration due to staining frequently occurs on the pits and fissures of teeth. Noncariogenic discoloration (non-CD) refers to the attachment of staining chromogens to sound surfaces, whereas cariogenic discoloration (CD) represents the discoloration of porous structures due to bacterial metabolites and mi...

journal_title：Journal of biomedical optics

authors： Lee HS,Kim SK,Park SW,de Josselin de Jong E,Kwon HK,Jeong SH,Kim BI

更新日期：2018-03-01 00:00:00

abstract：:Image resolution and signal level in fluorescence microscopy through inhomogeneous turbid media consisting of scatterers of multiple sizes under single- (1p), two- (2p), and three-photon (3p) excitation have been investigated based on a modified Monte Carlo model. The effects of the size distribution and the concentra...

journal_title：Journal of biomedical optics

authors： Deng X,Gan X,Gu M

更新日期：2003-07-01 00:00:00

abstract：:The development of methods that allow microscale studies of complex biomaterials based on their molecular composition is of great interest to a wide range of research fields. We show that stimulated Raman scattering (SRS) microscopy is an excellent analytical tool to study distributions of different biomolecules in mu...

journal_title：Journal of biomedical optics

authors： Roeffaers MB,Zhang X,Freudiger CW,Saar BG,van Ruijven M,van Dalen G,Xiao C,Xie XS

更新日期：2010-11-01 00:00:00

abstract：:Oblique incidence reflectometry (OIR) is an established technique for the estimation of tissue optical properties. However, a sensing footprint of a few transport mean-free paths is often needed when diffusion-regime-based algorithms are used. Smaller-footprint probes require improved light-propagation models and inve...

journal_title：Journal of biomedical optics

authors： Zemp RJ

更新日期：2013-06-01 00:00:00

abstract：:Coherent anti-Stokes Raman scattering (CARS) microscopy is used to determine the distribution and concentration of selected compounds in intact human hair. By generating images based on ratiometric CARS contrast, quantitative concentration maps of both water and externally applied d-glycine are produced in the cortex ...

journal_title：Journal of biomedical optics

authors： Zimmerley M,Lin CY,Oertel DC,Marsh JM,Ward JL,Potma EO

更新日期：2009-07-01 00:00:00

abstract：:Optical coherence tomography (OCT)-based elasticity imaging can map soft tissue elasticity based on speckle-tracking of elastic wave propagation using highly sensitive phase measurements of OCT signals. Using a fixed elastic wave source and moving detection, current imaging sequences have difficulty in reconstructing ...

journal_title：Journal of biomedical optics

authors： Hsieh BY,Song S,Nguyen TM,Yoon SJ,Shen TT,Wang RK,O'Donnell M

更新日期：2016-11-01 00:00:00

abstract：:Second-harmonic-generation (SHG) microscopy has emerged as a powerful tool to image unstained living tissues and probe their molecular and supramolecular organization. In this article, we review the physical basis of SHG, highlighting how coherent summation of second-harmonic response leads to the sensitivity of polar...

journal_title：Journal of biomedical optics

authors： Vanzi F,Sacconi L,Cicchi R,Pavone FS

更新日期：2012-06-01 00:00:00

abstract：:In the search for improved imaging modalities for detection and diagnosis of breast cancer, a high negative prediction value is also important. Photoacoustic (optoacoustic) imaging is a relatively new technique that has high potential for visualizing breast malignancies, but little is known about the photoacoustic app...

journal_title：Journal of biomedical optics

authors： Heijblom M,Piras D,Maartens E,Huisman EJ,van den Engh FM,Klaase JM,Steenbergen W,Manohar S

更新日期：2013-12-01 00:00:00

abstract：:Quantification of nicotinamide adenine dinucleotide (NADH) changes during functional brain activation and pathological conditions provides critical insight into brain metabolism. Of the different imaging modalities, two-photon laser scanning microscopy (TPLSM) is becoming an important tool for cellular-resolution meas...

journal_title：Journal of biomedical optics

authors： Baraghis E,Devor A,Fang Q,Srinivasan VJ,Wu W,Lesage F,Ayata C,Kasischke KA,Boas DA,Sakadzić S

更新日期：2011-10-01 00:00:00

abstract：:We report the design, integration, and validation of a fluorescence microscopy system for imaging of electroperturbation--the effects of nanosecond, megavolt-per-meter pulsed electric fields on biological cells and tissues. Such effects have potential applications in cancer therapy, gene regulation, and biophysical re...

journal_title：Journal of biomedical optics

authors： Sun Y,Vernier PT,Behrend M,Wang J,Thu MM,Gundersen M,Marcu L

更新日期：2006-03-01 00:00:00

abstract：:The measurement of changes in blood volume in tissue is important for monitoring the effects of a wide range of therapeutic interventions, from radiation therapy to skin-flap transplants. Many systems available for purchase are either expensive or difficult to use, limiting their utility in the clinical setting. A low...

journal_title：Journal of biomedical optics

authors： Glennie DL,Hayward JE,McKee DE,Farrell TJ

更新日期：2014-01-01 00:00:00

abstract：:Autofluorescence-based imaging techniques have become very important in the ophthalmological field. Being noninvasive and very sensitive, they are broadly used in clinical routines. Conventional autofluorescence intensity imaging is largely influenced by the strong fluorescence of lipofuscin, a fluorophore that can be...

journal_title：Journal of biomedical optics

authors： Sauer L,Andersen KM,Dysli C,Zinkernagel MS,Bernstein PS,Hammer M

更新日期：2018-09-01 00:00:00

abstract：:A new, simple, high-speed, and hardware-only integration-based fluorescence-lifetime-sensing algorithm using a center-of-mass method (CMM) is proposed to implement lifetime calculations, and its signal-to-noise-ratio based on statistics theory is also deduced. Compared to the commonly used iterative least-squares meth...

journal_title：Journal of biomedical optics

authors： Li DU,Rae B,Andrews R,Arlt J,Henderson R

更新日期：2010-01-01 00:00:00