Abstract：
:Analysis of the posttranslational modification of proteins, such as phosphorylation, might yield misleading results due to the presence of other proteins with similar electrophoretic properties that coimmunoprecipitate with the target protein. The aim of the present work was to develop a reliable, easy and economical technique to completely isolate a protein from its complex. Here we present a new assay developed to fully isolate proteins from macromolecular complexes that consists of an initial SDS/PAGE (under reducing conditions), which isolates the target protein, followed by transfer of the proteins to a buffer, from which the target protein is recaptured by conventional immunoprecipitation. This technique, that we have termed "Protein Complex Immunological Separation Assay" (ProCISA), successfully separated proteins of different sizes, such as pp60Src and the IP3 receptor (IP3R), from their complexes. We show that ProCISA allows the investigation of the tyrosine phosphorylation state of isolated proteins. This technique could also be used to study other posttranslational modifications without risk of misleading results resulting from contamination with other proteins of similar electrophoretic mobility which complex with the protein of interest.
journal_name
J Physiol Biochemjournal_title
Journal of physiology and biochemistryauthors
Redondo PC,Rosado JA,Salido GM,Sage SOdoi
10.1007/BF03178839subject
Has Abstractpub_date
2008-09-01 00:00:00pages
169-77issue
3eissn
1138-7548issn
1877-8755journal_volume
64pub_type
杂志文章abstract：:In spite of a wide range of drugs being available in the market, treatment of arterial hypertension still remains a challenge, and new therapeutic strategies could be developed in order to improve the rate of success in controlling this disease. Since oxidative stress has gained importance in the last few years as one...
journal_title：Journal of physiology and biochemistry
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doi：10.1007/s13105-010-0017-7
更新日期：2010-06-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章,评审
doi：10.1007/s13105-017-0564-2
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abstract：:The protective effects of downregulated miR-199a-5p on ischemic and hypoxic cardiomyocytes were well recognized, but the underlying mechanism of inhibited miR-199a-5p is not yet clear. The present study explored the relationship between enhanced signal transducer and activator of transcription 3 (STAT3) signaling and ...
journal_title：Journal of physiology and biochemistry
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doi：10.1007/s13105-015-0425-9
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pub_type： 杂志文章,评审
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doi：10.1007/s13105-013-0267-2
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更新日期：2013-09-01 00:00:00
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journal_title：Journal of physiology and biochemistry
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更新日期：2009-09-01 00:00:00
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journal_title：Journal of physiology and biochemistry
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doi：10.1007/s13105-010-0018-6
更新日期：2010-06-01 00:00:00
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journal_title：Journal of physiology and biochemistry
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更新日期：1998-06-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF03165968
更新日期：2009-03-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s13105-013-0269-0
更新日期：2013-12-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s13105-014-0363-y
更新日期：2014-12-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s13105-015-0442-8
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journal_title：Journal of physiology and biochemistry
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journal_title：Journal of physiology and biochemistry
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pub_type： 杂志文章
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更新日期：2017-05-01 00:00:00
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journal_title：Journal of physiology and biochemistry
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更新日期：2016-06-01 00:00:00
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doi：10.1007/s13105-015-0438-4
更新日期：2015-12-01 00:00:00
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journal_title：Journal of physiology and biochemistry
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更新日期：2016-12-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章,评审
doi：10.1007/BF03168378
更新日期：2005-12-01 00:00:00
abstract：:Growth hormone plays a key role in the maturation and maintenance of the immune response, however, the effects of chronic high circulating concentrations of the hormone on the immune system is poorly understood. Transgenic mice overexpressing bovine growth hormone (b-GH) gene, fused to the rat phosphoenolpyruvate carb...
journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF03179853
更新日期：2002-09-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章,评审
doi：10.1007/BF03179804
更新日期：2000-12-01 00:00:00
abstract：:The presence of metabolic syndrome (MS) per se or its separated components in HIV-infected patients contributes to an accelerated aging and increased cardiovascular risk. Gut microbiota (GM) dysbiosis has been linked with chronic inflammation associated with MS in a general non-infected population. However, no studies...
journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
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更新日期：2019-08-01 00:00:00
