Epidermal growth factor receptor is involved in clusterin-induced astrocyte proliferation.

Abstract:

:We previously reported that clusterin enhances astrocyte proliferation and extracellular signal-regulated kinase (ERK) activity. It, however, remains largely unknown how clusterin promotes cell growth. Here, we investigate the signaling pathway and related molecules underlying astrocyte proliferation by clusterin. Exogenous clusterin stimulates Ras-dependent Raf-1/mitogen-activated protein kinase kinase (MEK)/ERK activation. Clusterin-induced astrocyte proliferation and ERK1/2 phosphorylation were abrogated by either AG1478 (an inhibitor of epidermal growth factor receptor, EGFR) or EGFR small interfering RNA. Furthermore, clusterin treatment provoked tyrosine phosphorylation of EGFR (pY(1173)), which was also blocked by AG1478. These results suggest that clusterin requires EGFR activation to deliver its mitogenic signal through the Ras/Raf-1/MEK/ERK signaling cascade in astrocytes.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Shim YJ,Shin YJ,Jeong SY,Kang SW,Kim BM,Park IS,Min BH

doi

10.1097/WNR.0b013e3283262df8

subject

Has Abstract

pub_date

2009-03-04 00:00:00

pages

435-9

issue

4

eissn

0959-4965

issn

1473-558X

journal_volume

20

pub_type

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