Isolation of cadmium-induced DNA sequence in microalga Nannochloropsis oculata.

Abstract:

:The gene induced in response to Cd exposure in microalga Nannochloropsis oculata was examined in this investigation. In order to isolate this gene by using nested PCR, degenerated primers were designed on the basis of highly conserved regions of the amino acid sequences of various phytochelatin synthase. The size of the nested PCR amplification product from Nannochloropsis oculata by using two degenerated primers was measured to be 266 bp. The 266 bp DNA fragment was sequenced, and it might be induced by Cd exposure in microalga Nannochloropsis oculata.

journal_name

J Environ Biol

authors

Min JK,Rhim TY,Shin HW,Lee MY

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

457-60

issue

4

eissn

0254-8704

issn

2394-0379

journal_volume

29

pub_type

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