Abstract:
:An antiviral protein, RC28, with anti-herpesvirus activity was purified from a PBS extract of Rozites caperata (Cortinarious caperata) by acetone precipitation followed by gel filtration and ion exchange chromatography. The molecular weight of this protein was 28.251kDa as measured by MALDI-TOF mass spectrography. Our preparation of RC28 inhibited HSV-1 replication in vitro with an IC(50) value of 0.078mg/ml and a therapeutic index >32. The first 30 amino acid residues of RC28 were sequenced by Edman degradation to be MLTYRGKLNWYNYAVNEGFTLILPGXELKV. Based on that sequence, two degenerate primers were designed, the RC28 cDNA fragment was cloned by 3'-RACE, and the rest of the amino acid sequence was inferred from the cDNA sequence. The full-length peptide chain of RC28 has 235 amino acid residues, and was modified after its translation naturally. A search of the literature showed that this sequence has not been reported before and does not belong to any known protein family. A preliminary expression system was also constructed by inserting the cDNA into the PQE-30 vector, and transformed into Escherichia coli.
journal_name
Peptidesjournal_title
Peptidesauthors
Gong M,Piraino F,Yan N,Zhang J,Xia M,Ma J,Cheng J,Liu Xdoi
10.1016/j.peptides.2008.11.016subject
Has Abstractpub_date
2009-04-01 00:00:00pages
654-9issue
4eissn
0196-9781issn
1873-5169pii
S0196-9781(08)00492-0journal_volume
30pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::Melanin-concentrating hormone (MCH) is a potent orexigenic and sleep-promoting neuropeptide in mammals produced predominately by hypothalamic neurons which project to a wide variety of brain areas. Several MCH producing neurons contain MCH as the only neuropeptide, while others comprise cocaine- and amphetamine regula...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2017.11.002
更新日期:2018-01-01 00:00:00
abstract::Enzymatic cleavage of endogenous peptides is a commonly used principle to initiate, modulate and terminate action for instance among cytokines and peptide hormones. The incretin hormones, glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1), and the related hormone glucagon-like pepti...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2019.170224
更新日期:2020-03-01 00:00:00
abstract::Irisin was first identified in skeletal muscle cells, but its precise location has not yet been demonstrated, and there is limited information about irisin protein in other human and rat tissues. The present immunohistochemical study was undertaken to screen skeletal muscle and other tissues for irisin immunoreactivit...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2014.09.014
更新日期:2014-11-01 00:00:00
abstract::Pituitary cells from 14-day-old rats were separated by unit gravity velocity sedimentation, and a highly enriched population of gonadotrophs was established in reaggregate cell culture in serum-free and serum albumin-free defined culture media. The medium conditioned by these aggregates was concentrated, ultrafiltrate...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)90217-8
更新日期:1994-01-01 00:00:00
abstract::In the present study, the C-terminus of endomorphin (Tyr(1)-Pro(2)-Trp/Phe(3)-Phe(4)-NH(2), EMs) analogs [Xaa(4)-R]EMs, modified by substitution of a non-aromatic residue for Phe(4) and ending up with -NH-benzyl, were designed to generate an atypical conformationally constrained peptide set. We investigated the effect...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2007.01.002
更新日期:2007-04-01 00:00:00
abstract::ACTH-(1-24) inhibits the in vitro binding of the muscarinic antagonist [3H]QNB to membranes from rat brain. The magnitude of inhibition is dependent on the concentration of ACTH-(1-24). Kinetic analysis indicates a pure competitive inhibition which is suggestive of a reversible interaction of ACTH with muscarinic rece...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(86)90009-4
更新日期:1986-05-01 00:00:00
abstract::Dendroaspis natriuretic peptide (DNP) is one of four members of the natriuretic peptide family sharing functional and structural properties. The purpose of the present study was to elucidate the physiological role of DNP on renal functions and its cellular mechanism in the rabbit kidney. DNP (5 μg/kg/min) infused intr...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.12.006
更新日期:2012-01-01 00:00:00
abstract::The heptadecapeptide, orphanin FQ or nociceptin (Phe-Gly-Phe-Thr-Gly-Ala-Arg-Lys-Ser-Ala-Arg-Lys-Leu-Ala-Asn-Gln), originally isolated from rat brain has been identified as an endogenous ligand for the orphan opioid-like receptor. Although orphanin FQ shares some sequence and structural homology with kappa-opioid pept...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(97)00037-5
更新日期:1997-01-01 00:00:00
abstract::The teleost salmon calcitonin (sCT), but not mammalian CT, shows similar biologic actions in the skeletal muscle as amylin and calcitonin gene-related peptide (CGRP). The peptides have also been shown to reduce food intake in rams. Because sCT, but not amylin, binds irreversibly to amylin binding sites, the aim of the...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(99)00208-9
更新日期:2000-02-01 00:00:00
abstract::Natriuretic peptide receptor C (NPR-C) is known to bind all natriuretic peptides with similar affinity. Given their biological role it is interesting that natriuretic peptides and their activated guanylate cyclases (NPR-A and NPR-B) are expressed in retinal amacrine cells. The purpose of this study is to examine the p...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.10.014
更新日期:2010-01-01 00:00:00
abstract::High levels of neuropeptide Y (NPY) are found in the hypothalamus, median eminence, pituitary portal blood, and the pituitary in a number of species. Neuropeptide Y may influence the synthesis and secretion of a variety of hormones by interacting with specific receptors in the hypothalamus and/or the pituitary. To fur...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)90090-6
更新日期:1994-01-01 00:00:00
abstract::Increased plasma levels of neuropeptide Y (NPY)-like immunoreactivity (p-NPY-LI) have been described in hemodialysis (HD) patients. In this investigation the effect of a standard HD on p-NPY-LI, analyzed by radioimmunoassay and reverse-phase high performance liquid chromatography (HPLC), was studied. During dialysis p...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)00206-l
更新日期:1995-01-01 00:00:00
abstract::The reperfusion injury salvage kinase (RISK) pathway is a fundamental signal transduction cascade in the cardioprotective mechanism of ischemic postconditioning. In the present study, we examined the cardioprotective role of oxytocin as a postconditioning agent via activation of the RISK pathway (PI3K/Akt and ERK1/2)....
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2016.10.001
更新日期:2016-12-01 00:00:00
abstract::A novel protein expressed by entero-endocrine cells of the mouse stomach was named prepromotilin Related Peptide (ppMTLRP) since it shares sequence similarities with the prepromotilin (Tomasetto et al.). The mouse ppMTLRP was found identical to the rat precursor of ghrelin (ppghrelin), an endogenous ligand specific fo...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(01)00557-5
更新日期:2001-12-01 00:00:00
abstract::Cellular uptake of [125I] labelled DSIP at the luminal interface of the blood-brain barrier (BBB) was studied in the ipsilateral perfused in situ guinea pig forebrain. Regional unidirectional transfer constants (Kin) calculated from the multiple-time brain uptake analysis were 0.93, 1.33 and 1.66 microliter.min-1 g-1 ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(89)90026-0
更新日期:1989-03-01 00:00:00
abstract::Neuropeptide Y-containing nerve fibers have previously been demonstrated to innervate the mammalian ovary. These nerve fibers innervate primarily the vasculature. In this study we have developed a method for in vivo measurement of the ovary blood flow rate by means of the 133Xe method. Using this technique we measured...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(90)90041-3
更新日期:1990-05-01 00:00:00
abstract::Instability and proteolytic degradation limit the delivery options and in vivo efficacy of many therapeutic peptides. We previously generated a thioether stabilized angiotensin-(1-7) analog, cAng-(1-7), which is resistant against proteolytic degradation in the circulation. We here investigated oral and pulmonary deliv...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.02.015
更新日期:2010-05-01 00:00:00
abstract::We have studied the in vitro effect of VIP and histamine on ultrastructure of the parietal cells in isolated guinea pig fundic glands. The morphological changes induced by histamine in the parietal cells can be compared to those observed after histamine stimulation in vivo or in vitro on gastric mucosa preparations. I...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(84)90219-5
更新日期:1984-03-01 00:00:00
abstract::To study the effects of inducing stereo-chemical modifications in the structure of dermorphin (DM) so as to improve its mu-opioid receptor affinity and its resistance to C-terminal enzymatic degradation, in the Institute of Molecular Genetics of Moscow, we synthesized a new DM analogue ([DPro(6)]DM) and analyzed the c...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(03)00057-3
更新日期:2003-03-01 00:00:00
abstract::Our previous findings that the degree of endotoxin-induced hypotension in the dog is inversely related to ambient temperature (19 degrees through 30 degrees C) and that only increased doses of naloxone are effective at 19 degrees C suggested that opioid activity is also influenced by ambient temperature, increasing in...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(85)90007-5
更新日期:1985-01-01 00:00:00
abstract::Insect flight requires rapid mobilization of energy reserves during flight, which is mediated and regulated by hormonal control via adipokinetic hormones. The structure of the G-protein receptors to which these hormones bind, are crucial in understanding many of the physiological processes in which they play a central...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.08.017
更新日期:2011-03-01 00:00:00
abstract::Antimicrobial peptides allegedly exert their action on microbial membranes. Bovine lactoferrin enfold two antimicrobial domains, lactoferricin B (LFcin B) and lactoferrampin (LFampin). Effects of representative peptides thereof on the membranes of Candida albicans and Escherichia coli were investigated. Confocal laser...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.02.011
更新日期:2005-09-01 00:00:00
abstract::HP-1 is the most abundant human representative of a recently discovered class of neutrophil cystine- and arginine-rich peptides. These peptides have many potentially regulatory activities expressed at nanomolar concentrations. To establish the levels of HP-1 that can accumulate in human lung tumors and nondiseased lun...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(92)90152-s
更新日期:1992-01-01 00:00:00
abstract::This study was performed to identify the efferents of the dorsal raphe nucleus (DRN), particularly in regard to nociception. Phaseolus vulgaris leucoagglutinin (PHA-L) was microiontophoresed into the DRN and visualized immunocytochemically; PHA-L-immunoreactive (-IR) fibers and terminals were identified in the forebra...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90013-7
更新日期:1993-01-01 00:00:00
abstract::One novel Kunitz BPTI-like peptide designated as BBPTI-1, with chymotrypsin inhibitory activity was identified from the venom of Burmese Daboia russelii siamensis. It was purified by three steps of chromatography including gel filtration, cation exchange and reversed phase. A partial N-terminal sequence of BBPTI-1, HD...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2013.02.009
更新日期:2013-05-01 00:00:00
abstract::While neuropeptides of the allatostatin family inhibit in vitro production of juvenile hormone, which modulates aspects of development and reproduction in the cockroach, Diploptera punctata, they are susceptible to inactivation by peptidases in the hemolymph, gut, and bound to internal tissues. Patterns of peptidase c...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(98)00149-1
更新日期:1999-01-01 00:00:00
abstract::beta-Lactotensin (beta-LT) is a bioactive peptide derived from bovine milk beta-lactoglobulin and is a natural ligand for neurotensin receptors. We examined the effect of beta-LT on restraint stress and fear memory in mice. Mice subjected to acute restraint stress exhibited a decreased number of head-dips and increase...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2006.08.009
更新日期:2006-12-01 00:00:00
abstract::The aim of the present study was to investigate how suckling influences the plasma levels of oxytocin and vasoactive intestinal peptide in lactating dogs. Blood-samples were drawn from ten lactating beagles during suckling, in week one and week three of the five week long lactation period and the levels of vasoactive ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(87)90002-7
更新日期:1987-05-01 00:00:00
abstract::The effects of atrial natriuretic factor (ANF) on [3H]norepinephrine ([3H]NE) release evoked by a sodium-free medium (SFM) were studied. Experiments were performed in rat adrenal medulla slices incubated in vitro. Results showed that [3H]NE release evoked by the omission of Na+ was decreased by 10 nM ANF. In addition,...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)90100-7
更新日期:1994-01-01 00:00:00
abstract::The RPCH and beta-actin cDNAs from the crayfish Cherax quadricarinatus were amplified, cloned and sequenced. The primary structure sequences of these cDNAs were compared to other members of the AKH/RPCH family. Fluctuations in the amount of the C. quadricarinatus RPCH and beta-actin mRNAs, as cDNAs, were quantified ev...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.05.018
更新日期:2005-12-01 00:00:00