Identification of Mycobacterium tuberculosis vaccine candidates using human CD4+ T-cells expression cloning.


:To identify Mycobacterium tuberculosis (Mtb) antigens as candidates for a subunit vaccine against tuberculosis (TB), we have employed a CD4+ T-cell expression screening method. Mtb-specific CD4+ T-cell lines from nine healthy PPD positive donors were stimulated with different antigenic substrates including autologous dendritic cells (DC) infected with Mtb, or cultured with culture filtrate proteins (CFP), and purified protein derivative of Mtb (PPD). These lines were used to screen a genomic Mtb library expressed in Escherichia coli and processed and presented by autologous DC. This screening led to the recovery of numerous T-cell antigens, including both novel and previously described antigens. One of these novel antigens, referred to as Mtb9.8 (Rv0287), was recognized by multiple T-cell lines, stimulated with either Mtb-infected DC or CFP. Using the mouse and guinea pig models of TB, high levels of IFN-gamma were produced, and solid protection from Mtb challenge was observed following immunization with Mtb9.8 formulated in either AS02A or AS01B Adjuvant Systems. These results demonstrate that T-cell screening of the Mtb genome can be used to identify CD4+ T-cell antigens that are candidates for vaccine development.






Coler RN,Dillon DC,Skeiky YA,Kahn M,Orme IM,Lobet Y,Reed SG,Alderson MR




Has Abstract


2009-01-07 00:00:00
















  • Preparation and evaluation of antigen/N-trimethylaminoethylmethacrylate chitosan conjugates for nasal immunization.

    abstract::The frequent outbreak of respiratory infectious diseases such as influenza and pulmonary tuberculosis calls for new immunization strategies with high effectiveness. Nasal immunization is one of the most potential methods to prevent the diseases infected through the respiratory tract. In this study, we designed a water...


    pub_type: 杂志文章


    authors: Liu Q,Zhang C,Zheng X,Shao X,Zhang X,Zhang Q,Jiang X

    更新日期:2014-05-07 00:00:00

  • Placebo-controlled efficacy study of hepatitis A vaccine in Valdivia, Chile.

    abstract::A placebo-controlled, double-blind study on the efficacy of a hepatitis A vaccine (SmithKline Beecham Biologicals) was started in a region of Chile in September 1990, using hepatitis B vaccine as control. A total of 260 healthy children, 6-15 years of age, negative for antibody to hepatitis A virus (anti-HAV), antibod...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Riedemann S,Reinhardt G,Frösner GG,Ibarra H,Moraleda L,Hering V,Siegel F,Toledo C,Leon J,Gonzalez JL

    更新日期:1992-01-01 00:00:00

  • Novel polysaccharide adjuvant from the roots of Actinidia eriantha with dual Th1 and Th2 potentiating activity.

    abstract::The plant polysaccharides are recognized as an effective biological response modifier with low toxicity. In this study, the water-soluble polysaccharide from the roots of Actinidia eriantha (AEPS) was evaluated for its toxicity and adjuvant potential on the specific cellular and humoral immune responses to ovalbumin (...


    pub_type: 杂志文章


    authors: Sun HX,Wang H,Xu HS,Ni Y

    更新日期:2009-06-19 00:00:00

  • Cloning and assessment of tumorigenicity and oncogenicity of a Madin-Darby canine kidney (MDCK) cell line for influenza vaccine production.

    abstract::An Madin-Darby canine kidney (MDCK) cell line, 9B9-1E4, was cloned by limit dilution from a heterologous cell population and chosen as a potential production cell substrate for cell culture-based influenza vaccine manufacture. Since MDCK cells are transformed cells of canine origin, extensive characterization, includi...


    pub_type: 杂志文章


    authors: Liu J,Mani S,Schwartz R,Richman L,Tabor DE

    更新日期:2010-02-03 00:00:00

  • Impact of hepatitis B vaccination in a highly endemic area of south Italy and long-term duration of anti-HBs antibody in two cohorts of vaccinated individuals.

    abstract::The aims of this study were to evaluate the impact of hepatitis B vaccination on the changing pattern of HBV infection in a former hyperendemic area (Afragola, South Italy), and to assess the long-term persistence of anti-HBs in two cohorts of individuals vaccinated as infants 18 and 23 years ago. Our data shows a sig...


    pub_type: 杂志文章


    authors: Da Villa G,Romanò L,Sepe A,Iorio R,Paribello N,Zappa A,Zanetti AR

    更新日期:2007-04-20 00:00:00

  • Capture EIA for determination of IgE to diphtheria and tetanus toxins in comparison to RIA.

    abstract::A capture IgE assay in EIA with diphtheria toxin and tetanus toxoid as model systems was developed and evaluated in relation to RIA in a DT booster study. The EIA and the RIA gave very similar results, although the EIA determined IgE over a wider range of concentrations, without interference by other subclasses. The c...


    pub_type: 杂志文章


    authors: Wahren E,Bengtsson C,Björkstén B,Mark A,Granström M

    更新日期:1998-05-01 00:00:00

  • Safety and immunogenicity of a high dosage trivalent influenza vaccine among elderly subjects.

    abstract::To improve immune responses to influenza vaccine, a trivalent inactivated vaccine containing 60 microg of the HA of each component (A/H3N2, A/H1N1, B) was compared to a licensed vaccine containing 15 microg of the HA of each. More local and systemic reactions were reported by subjects given the high dosage but only lo...


    pub_type: 杂志文章,随机对照试验


    authors: Couch RB,Winokur P,Brady R,Belshe R,Chen WH,Cate TR,Sigurdardottir B,Hoeper A,Graham IL,Edelman R,He F,Nino D,Capellan J,Ruben FL

    更新日期:2007-11-01 00:00:00

  • DNA vaccination with plasmids containing various fragments of large segment genome of infectious bursal disease virus.

    abstract::The present study was undertaken to determine the effectiveness in including VP2 gene of the large segment genome of infectious bursal disease virus (IBDV) in the DNA vaccine for protection of chickens against infectious bursal disease (IBD). Different fragments of the large segment gene of IBDV standard challenge (ST...


    pub_type: 杂志文章


    authors: Chang HC,Lin TL,Wu CC

    更新日期:2003-01-17 00:00:00

  • Efficacious, nontoxigenic Bacillus anthracis spore vaccines based on strains expressing mutant variants of lethal toxin components.

    abstract::We reported previously on the development of a Bacillus anthracis vaccine strain expressing high levels of recombinant protective antigen (rPA) [Cohen et al., Infec Immun 2000;68(8):4549-58]. To further explore the potential of the B. anthracis platform, we generated several attenuated strains expressing lethal toxin ...


    pub_type: 杂志文章


    authors: Mendelson I,Gat O,Aloni-Grinstein R,Altboum Z,Inbar I,Kronman C,Bar-Haim E,Cohen S,Velan B,Shafferman A

    更新日期:2005-12-01 00:00:00

  • Influenza vaccination among U.S. pediatric patients receiving care from federally funded health centers.

    abstract:INTRODUCTION:During the 2018-2019 influenza season, vaccination coverage among U.S. children was 62.6%. The purpose of this study was to estimate the prevalence of influenza vaccinations among pediatric patients seen in U.S. health centers, and to explore potential disparities in vaccination coverage among subpopulatio...


    pub_type: 杂志文章


    authors: Lebrun-Harris LA,Mendel Van Alstyne JA,Sripipatana A

    更新日期:2020-09-03 00:00:00

  • Immunization of human hepatitis E viruses conferred protection against challenge by a camel hepatitis E virus.

    abstract::Dromedary camel hepatitis E virus is a novel HEV that belongs to the family Hepeviridae, and is classified as genotype 7 HEV (HEV-7). Since HEV-7 is transmitted from camels to humans and causes acute hepatitis E, this virus is a non-negligible pathogen for zoonosis, and a vaccine against HEV-7 infection is urgently ne...


    pub_type: 杂志文章


    authors: Guo Y,Yang F,Xu X,Feng M,Liao Y,He Z,Takeda N,Muramatsu M,Li Q,Li TC

    更新日期:2020-10-27 00:00:00

  • The immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, is a highly protective antigen against serotype 2.

    abstract::Streptococcus suis (S. suis) is a major porcine pathogen causing meningitis, arthritis and several other pathologies. Recently, we identified a highly specific immunoglobulin M degrading enzyme of S. suis, designated IdeSsuis, which is expressed by various serotypes. The objective of this work was to access the immuno...


    pub_type: 杂志文章


    authors: Seele J,Hillermann LM,Beineke A,Seitz M,von Pawel-Rammingen U,Valentin-Weigand P,Baums CG

    更新日期:2015-05-05 00:00:00

  • Feasibility of a pilot intervention to reduce pain and syncope during adolescent vaccination.

    abstract:PURPOSE:Vaccines recommended for adolescents are considered safe and effective, however administration may occasionally result in acute pain at the injection site or syncope (fainting). These adverse effects pose a risk to patient safety and are potential barriers to adherence to future vaccinations. We assessed a nove...


    pub_type: 杂志文章


    authors: Henninger ML,Kuntz JL,Firemark AJ,Varga AM,Bok K,Naleway AL

    更新日期:2018-06-22 00:00:00

  • Achieving a "Grand Convergence" in global health by 2035.

    abstract::Historical evidence demonstrates the validity of the concept "Grand Convergence". The Lancet commission identified the major challenges facing low and lower middle income countries including the unfinished agenda, non-communicable diseases and injuries and an approach to funding and achieving these objectives along wi...


    pub_type: 杂志文章


    authors: Mahmoud A

    更新日期:2017-01-20 00:00:00

  • Immunisation of chickens with live Salmonella vaccines - Role of booster vaccination.

    abstract::It is accepted that booster vaccinations of chickens with live Salmonella vaccines are essential part of vaccinations schemes to induce an effective adaptive immune response. As manufacturer of registered live Salmonella vaccines recommend different times of booster the question raises whether the duration between the...


    pub_type: 杂志文章


    authors: Methner U

    更新日期:2018-05-17 00:00:00

  • Seroprevalence of anti-polio antibodies in a population 7 months to 39 years of age in Uruguay: Implications for future polio vaccination strategies.

    abstract::This study evaluated the seroprevalence of poliovirus types 1, 2 and 3 antibodies and vaccination coverage in 780 subjects aged between 7 months and 39 years in Montevideo, Uruguay, where oral polio vaccine (OPV) is used. Antibody titers were measured and seroprotection rates and geometric mean titers (GMTs) were comp...


    pub_type: 杂志文章


    authors: Pírez MC,Olivera I,Diabarboure H,Montano A,Barañano R,Badía F,Bonnet MC

    更新日期:2009-05-05 00:00:00

  • Analysis of adenovirus-induced immunity to infection with Listeria monocytogenes: Fading protection coincides with declining CD8 T cell numbers and phenotypic changes.

    abstract::Defining correlates of T cell mediated protection is important in order to accelerate the development of efficient T cell based vaccines conferring long-term immunity. Extensive studies have provided important insight regarding the characteristics and functional properties of the effector and memory CD8 T cells induce...


    pub_type: 杂志文章


    authors: Jahn ML,Steffensen MA,Christensen JP,Thomsen AR

    更新日期:2018-05-11 00:00:00

  • Vaccination with a preparation based on recombinant cysteine peptidases and canine IL-12 does not protect dogs from infection with Leishmania infantum.

    abstract::Cysteine peptidases (CPs) have been implicated in various processes central to the pathogenicity of Leishmania parasites, and are thought to be key factors in the host-parasite interaction. In order to fully evaluate the potential of the CPs as vaccine candidates, studies in natural host species are required. In the s...


    pub_type: 杂志文章


    authors: Poot J,Spreeuwenberg K,Sanderson SJ,Schijns VE,Mottram JC,Coombs GH,Vermeulen AN

    更新日期:2006-03-24 00:00:00

  • The science of vaccine safety: Summary of meeting at Wellcome Trust.

    abstract::Vaccines are everywhere hugely successful but are also under attack. The reason for the latter is the perception by some people that vaccines are unsafe. However that may be, vaccine safety, life any other scientific subject, must be constantly studied. It was from this point of view that a meeting was organized at th...


    pub_type: 杂志文章


    authors: Plotkin SA,Offit PA,DeStefano F,Larson HJ,Arora NK,Zuber PLF,Fombonne E,Sejvar J,Lambert PH,Hviid A,Halsey N,Garçon N,Peden K,Pollard AJ,Markowitz LE,Glanz J

    更新日期:2020-02-18 00:00:00

  • Attitudes and perceptions of private pediatricians regarding polio immunization in India.

    abstract:BACKGROUND:India has faced considerable challenges in eradicating polio. Uttar Pradesh (UP) and Bihar are the two states in India where transmission of polio has never been interrupted. Private pediatricians are important stakeholders for vaccine delivery and maintaining public confidence in vaccines. The purpose of th...


    pub_type: 杂志文章


    authors: Choudhury P,Thacker N,Gargano LM,Weiss PS,Vashishtha VM,Amladi T,Pazol K,Orenstein WA,Omer SB,Hughes JM

    更新日期:2011-10-26 00:00:00

  • Contrasting substitution patterns between HA proteins of avian and human influenza viruses: Implication for monitoring human influenza epidemics.

    abstract::The HA1 domain of influenza A viruses is critical for immune escape and host receptor recognition. However, the association between HA1 evolution and host species-specific adaptations remains unclear. We have identified the amino acid sites that are related to the host species-specific adaptations by systematically co...


    pub_type: 杂志文章


    authors: Liao YC,Chen FC,Hsiung CA

    更新日期:2010-11-23 00:00:00

  • Bordetella pertussis iron regulated proteins as potential vaccine components.

    abstract::Bordetella pertussis is the etiologic agent of whooping cough, an illness whose incidence has been increasing over the last decades. Pertussis reemergence despite high vaccination coverage, together with the recent isolation of circulating strains deficient in some of the vaccine antigens, highlight the need for new v...


    pub_type: 杂志文章


    authors: Alvarez Hayes J,Erben E,Lamberti Y,Principi G,Maschi F,Ayala M,Rodriguez ME

    更新日期:2013-08-02 00:00:00

  • Hepatitis A and B in children and adolescents--what can we learn from Puglia (Italy) and Catalonia (Spain)?

    abstract::Viral hepatitis remains a major contributor to the global disease burden. Mass immunisation strategies against hepatitis B have been adopted by more than 90 developing and industrialised countries. Countries with low hepatitis A endemicity are experiencing cyclical outbreaks and an epidemiological shift, with larger n...


    pub_type: 杂志文章


    authors: Lopalco PL,Salleras L,Barbuti S,Germinario C,Bruguera M,Buti M,Domínguez A

    更新日期:2000-10-15 00:00:00

  • A qualitative analysis of the impact of healthcare personnel influenza vaccination requirements in California.

    abstract:OBJECTIVE:Using qualitative methods, we explored the implementation of California's 2007 influenza immunization requirements of hospital-based health care personnel (HCP). METHODS:We conducted nine case studies of California hospitals with different HCP vaccination rates and policies. Case studies consisted of intervi...


    pub_type: 杂志文章


    authors: Khodyakov D,Uscher-Pines L,Lorick SA,Lindley MC,Shier V,Harris K

    更新日期:2014-05-23 00:00:00

  • Immunization with DnaJ (hsp40) could elicit protection against nasopharyngeal colonization and invasive infection caused by different strains of Streptococcus pneumoniae.

    abstract::Increasing mortality, morbidity and economic costs have been paid to pneumococcal diseases every year. Currently, vaccination is the most promising strategy to reduce the occurrence of pneumococcal infection. In this study, we investigated the protective efficacy of immunization with recombinant DnaJ (hsp40) protein a...


    pub_type: 杂志文章


    authors: Cui Y,Zhang X,Gong Y,Niu S,Yin N,Yao R,Xu W,Li D,Wang H,He Y,Cao J,Yin Y

    更新日期:2011-02-17 00:00:00

  • Impact of influenza vaccination on mortality in the French elderly population during the 2000-2009 period.

    abstract::With declining influenza vaccine coverage in the elderly in recent years in France, we aimed at assessing the benefits of seasonal influenza vaccination, based on available data for observed mortality, vaccine coverage and vaccine effectiveness. To estimate the annual number of deaths avoided by vaccination in the peo...


    pub_type: 杂志文章


    authors: Bonmarin I,Belchior E,Lévy-Bruhl D

    更新日期:2015-02-25 00:00:00

  • Prevention of neonatal tolerance by a plasmid encoding granulocyte-macrophage colony stimulating factor.

    abstract::A plasmid DNA vaccine encoding the circumsporozoite protein of malaria (pCSP) induces protective immunity in adult mice but persistent tolerance when administered to neonates. In an effort to improve antigen presenting cell (APC) function in newborns, we co-administered pCSP with a plasmid encoding granulocyte-macroph...


    pub_type: 杂志文章


    authors: Ishii KJ,Weiss WR,Klinman DM

    更新日期:1999-11-12 00:00:00

  • The effect of diabetes mellitus on immunological response to hepatitis B virus vaccine in individuals with chronic kidney disease: A meta-analysis of current literature.

    abstract:BACKGROUND:Patients with chronic kidney disease (CKD) often fail to produce protective antibodies to hepatitis B virus (HBV) surface antigen after vaccination. Diabetes mellitus (DM) is the most common cause of CKD; however it is not clear whether it affects immunological response to HBV vaccine in these patients. AIM...


    pub_type: 杂志文章,meta分析,评审


    authors: Alavian SM,Tabatabaei SV

    更新日期:2010-05-14 00:00:00

  • Safety and immunogenicity of fully liquid and lyophilized formulations of an investigational trivalent group B streptococcus vaccine in healthy non-pregnant women: Results from a randomized comparative phase II trial.

    abstract:BACKGROUND:We evaluated the safety and immunogenicity of liquid and lyophilized formulations of an investigational trivalent group B streptococcus (GBS) vaccine in non-pregnant women and assessed the formulations' equivalence in terms of serotype-specific immune response. METHODS:This phase II, randomized, comparative...


    pub_type: 杂志文章


    authors: Beran J,Leroux-Roels G,Van Damme P,de Hoon J,Vandermeulen C,Al-Ibrahim M,Johnson C,Peterson J,Baker S,Seidl C,Dreisbach A,Karsten A,Corsaro B,Henry O,Lattanzi M,Bebia Z

    更新日期:2020-04-03 00:00:00

  • Rabies post-exposure prophylaxis vaccination with purified chick embryo cell vaccine (PCECV) and purified Vero cell rabies vaccine (PVRV) in a four-site intradermal schedule (4-0-2-0-1-1): an immunogenic, cost-effective and practical regimen.

    abstract::Currently, two intradermal (ID) regimens for rabies post-exposure prophylaxis (PEP) are recommended by WHO and used in countries where approved by national authorities: the Thai Red Cross (TRC) two-site ID regimen and the eight-site ID regimen. Besides these WHO recommended schedules, a new economical four-site ID reg...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Ambrozaitis A,Laiskonis A,Balciuniene L,Banzhoff A,Malerczyk C

    更新日期:2006-05-08 00:00:00