A novel E. coli biosensor for detecting aromatic aldehydes based on a responsive inducible archaeal promoter fused to the green fluorescent protein.

Abstract:

:A whole-cell bacterial biosensor for measuring aqueous concentrations of aromatic aldehydes was developed. It is based on the E. coli BL21DE3(RIL) expressing the green fluorescent protein under the control of an alcohol dehydrogenase inducible promoter belonging to the archaeon Sulfolobus solfataricus (Sso2536adh promoter). Since it was previously reported that the BldR regulatory protein is the transcription factor required for aromatic aldehyde response in S. solfataricus, the gene encoding for the sensor protein BldR was co-expressed in the biosensor strain on a different compatible plasmid. Gel mobility shift assays showed that the purified recombinant protein can bind specifically to the Sso2536adh promoter. We demonstrated the ability of the archaeal promoter and the BldR transcription factor to operate in a bacterial context to drive active gene expression in a hybrid archaeal/eukaryal fusion. Furthermore, the E. coli BL21DE3(RIL) biosensor strain displayed a specific response and high sensitivity to the different aromatic aldehydes used, suggesting its potential low-cost application to environmentally relevant samples.

authors

Fiorentino G,Ronca R,Bartolucci S

doi

10.1007/s00253-008-1771-0

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

67-77

issue

1

eissn

0175-7598

issn

1432-0614

journal_volume

82

pub_type

杂志文章
  • Extrachromosomal, extraordinary and essential--the plasmids of the Roseobacter clade.

    abstract::The alphaproteobacterial Roseobacter clade (Rhodobacterales) is one of the most important global players in carbon and sulfur cycles of marine ecosystems. The remarkable metabolic versatility of this bacterial lineage provides access to diverse habitats and correlates with a multitude of extrachromosomal elements. Fou...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-013-4746-8

    authors: Petersen J,Frank O,Göker M,Pradella S

    更新日期:2013-04-01 00:00:00

  • Bioaugmentation and enhanced formation of microbial granules used in aerobic wastewater treatment.

    abstract::Microbial aggregates of an aerobic granular sludge can be used for the treatment of industrial or municipal wastewater, but their formation from a microbial activated sludge requires several weeks. Therefore, the aim of this research was the selection of microbial cultures to shorten the granule-forming period from se...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-005-0088-5

    authors: Ivanov V,Wang XH,Tay ST,Tay JH

    更新日期:2006-04-01 00:00:00

  • Screening of a novel strong promoter by RNA sequencing and its application to H2 production in a hyperthermophilic archaeon.

    abstract::A strong promoter increases transcription of the genes of interest and enhances the production of various valuable substances. For a hyperthermophilic archaeon Thermococcus onnurineus NA1, which can produce H2 from carbon monoxide oxidation, we searched for a novel endogenous strong promoter by transcriptome analysis ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-6444-1

    authors: Lee SH,Kim MS,Jung HC,Lee J,Lee JH,Lee HS,Kang SG

    更新日期:2015-05-01 00:00:00

  • Aerobic dechlorination of low-chlorinated biphenyls by bacterial biofilms in packed-bed batch bioreactors.

    abstract::Cells of an aerobic three-membered bacterial co-culture, designated as ECO3, capable of cometabolizing and aerobically dechlorinating low-chlorinated biphenyls in the presence of biphenyl, were immobilized on Manville silica beads, on frosted-glass beads and on polyurethane foam cubes in packed-bed bioreactors continu...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00578472

    authors: Fava F,Di Gioia D,Marchetti L,Quattroni G

    更新日期:1996-05-01 00:00:00

  • Optimization of the extracellular production of a bacterial phytase with Escherichia coli by using different fed-batch fermentation strategies.

    abstract::The extracellular production of Escherichia coli phytase was studied in fed-batch fermentations. Two different feeding strategies were compared: control by keeping the glucose concentration constant, and control by keeping a low constant oxygen level in the medium. For the feeding control based on glucose concentratio...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-003-1229-3

    authors: Kleist S,Miksch G,Hitzmann B,Arndt M,Friehs K,Flaschel E

    更新日期:2003-06-01 00:00:00

  • Characterization of bacterial and archaeal communities in air-cathode microbial fuel cells, open circuit and sealed-off reactors.

    abstract::A large percentage of organic fuel consumed in a microbial fuel cell (MFC) is lost as a result of oxygen transfer through the cathode. In order to understand how this oxygen transfer affects the microbial community structure, reactors were operated in duplicate using three configurations: closed circuit (CC; with curr...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-5025-4

    authors: Shehab N,Li D,Amy GL,Logan BE,Saikaly PE

    更新日期:2013-11-01 00:00:00

  • Efficient enzymatic systems for synthesis of novel α-mangostin glycosides exhibiting antibacterial activity against Gram-positive bacteria.

    abstract::Two enzymatic systems were developed for the efficient synthesis of glycoside products of α-mangostin, a natural xanthonoid exhibiting anti-oxidant, antibacterial, anti-inflammatory, and anticancer activities. In these systems, one-pot reactions for the synthesis of UDP-α-D-glucose and UDP-α-D-2-deoxyglucose were modi...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5947-5

    authors: Le TT,Pandey RP,Gurung RB,Dhakal D,Sohng JK

    更新日期:2014-10-01 00:00:00

  • Biocatalytic synthesis of chiral intermediate of pregabalin with high substrate loading by a newly isolated Morgarella morganii ZJB-09203.

    abstract::The chemoenzymatic process involving biocatalytic resolution of rac-2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester (CNDE, 1) has been the most competitive and attractive route for pregabalin. A new esterase-producing strain ZJB-09203, which exhibited high hydrolytic activity, excellent enantioselectivity, an...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-4810-4

    authors: Zheng RC,Wang TZ,Fu DJ,Li AP,Li XJ,Zheng YG

    更新日期:2013-06-01 00:00:00

  • LysA2, the Lactobacillus casei bacteriophage A2 lysin is an endopeptidase active on a wide spectrum of lactic acid bacteria.

    abstract::The lysin gene (lysA2) of the Lactobacillus casei bacteriophage A2 was cloned and expressed in Escherichia coli. LysA2 is an endopeptidase that hydrolyzes the bond between the terminal D: -alanine of the peptidoglycan tetrapeptide and the aspartic acid residue that forms the bridge with the L: -lysine of a neighboring...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3588-5

    authors: Ribelles P,Rodríguez I,Suárez JE

    更新日期:2012-04-01 00:00:00

  • Microbial communities from 20 different hydrogen-producing reactors studied by 454 pyrosequencing.

    abstract::To provide new insight into the dark fermentation process, a multi-lateral study was performed to study the microbiology of 20 different lab-scale bioreactors operated in four different countries (Brazil, Chile, Mexico, and Uruguay). Samples (29) were collected from bioreactors with different configurations, operation...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7325-y

    authors: Etchebehere C,Castelló E,Wenzel J,del Pilar Anzola-Rojas M,Borzacconi L,Buitrón G,Cabrol L,Carminato VM,Carrillo-Reyes J,Cisneros-Pérez C,Fuentes L,Moreno-Andrade I,Razo-Flores E,Filippi GR,Tapia-Venegas E,Toledo-Alarcón J

    更新日期:2016-04-01 00:00:00

  • High-cell-density cultivation of recombinant Escherichia coli, purification and characterization of a self-sufficient biosynthetic octane ω-hydroxylase.

    abstract::We have recently described the biocatalytic characterization of a self-sufficent biosynthetic alkane hydroxylase based on CYP153A13a from Alcanivorax borkumensis SK2 (thereafter A13-Red). Despite remarkable regio- and chemo-selectivity, A13-Red suffers of a difficult-to-reproduce expression and moderate operational st...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5671-1

    authors: Bordeaux M,de Girval D,Rullaud R,Subileau M,Dubreucq E,Drone J

    更新日期:2014-01-01 00:00:00

  • Genetically engineered fusion of allergen and viral-like particle induces a more effective allergen-specific immune response than a combination of them.

    abstract::Chimeric virus-like particles (VLPs) were developed as a candidate for allergen-specific immunotherapy. In this study, hepatitis B core antigen (HBcAg) that genetically fused to Chenopodium album polcalcin (Che a 3)-derived peptide was expressed in E. coli BL21, purified, and VLP formation was evaluated using native a...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-11012-0

    authors: Sani MZ,Bargahi A,Momenzadeh N,Dehghani P,Moghadam MV,Maleki SJ,Nabipour I,Shirkani A,Akhtari J,Hesamizadeh K,Heidari S,Omrani F,Akbarzadeh S,Mohammadi M

    更新日期:2021-01-01 00:00:00

  • Incorporation of non-standard amino acids into proteins: challenges, recent achievements, and emerging applications.

    abstract::The natural genetic code only allows for 20 standard amino acids in protein translation, but genetic code reprogramming enables the incorporation of non-standard amino acids (NSAAs). Proteins containing NSAAs provide enhanced or novel properties and open diverse applications. With increased attention to the recent adv...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-019-09690-6

    authors: Jin X,Park OJ,Hong SH

    更新日期:2019-04-01 00:00:00

  • Review of research on Dendrobium, a prized folk medicine.

    abstract::Medicinal plants of the Dendrobium genus are highly prized, and hence, methodologies have been developed to authenticate Dendrobium drugs from its adulterants. Many bioactive constituents of Dendrobium species have been identified. The macromolecules included lectins; the enzymes chalcone synthase, sucrose synthase, a...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-011-3829-7

    authors: Ng TB,Liu J,Wong JH,Ye X,Wing Sze SC,Tong Y,Zhang KY

    更新日期:2012-03-01 00:00:00

  • Production of a novel compound, 10,12-dihydroxystearic acid from ricinoleic acid by an oleate hydratase from Lysinibacillus fusiformis.

    abstract::A recombinant oleate hydratase from Lysinibacillus fusiformis converted ricinoleic acid to a product, whose chemical structure was identified as the novel compound 10,12-dihydroxystearic acid by gas chromatograph/mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance analysis. The reaction condi...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-4728-x

    authors: Seo MH,Kim KR,Oh DK

    更新日期:2013-10-01 00:00:00

  • Improving mycelial morphology and adherent growth as well as metabolism of Monascus yellow pigments using nitrate resources.

    abstract::Mycelial adhesion affects cell growth and the production of water-soluble extracellular yellow pigment (EYP) in submerged fermentation with Monascus ruber CGMCC 10910. Two nitrates, NaNO3 and KNO3, were used as nitrogen sources for mitigating mycelial adhesion and improving the production of EYP in this study. The res...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10944-x

    authors: Yang SZ,Huang ZF,Liu HQ,Hu X,Wu ZQ

    更新日期:2020-11-01 00:00:00

  • Soil acidification amendments change the rhizosphere bacterial community of tobacco in a bacterial wilt affected field.

    abstract::Application of soil amendments has been wildly used to increase soil pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community of crops, especially when the field naturally harbors the disease of bacterial wilt to tobacco for many years due to long-term continuo...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-9347-0

    authors: Shen G,Zhang S,Liu X,Jiang Q,Ding W

    更新日期:2018-11-01 00:00:00

  • Biophotography: concepts, applications and perspectives.

    abstract::Synthetic biology aims at manipulating biological systems by rationally designed and genetically introduced components. Efforts in photoactuator engineering resulted in microorganisms reacting to extracellular light-cues with various cellular responses. Some of them lead to the formation of macroscopically observable ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-016-7384-0

    authors: Renicke C,Taxis C

    更新日期:2016-04-01 00:00:00

  • Heterologous expression and characterization of bacterial 2-C-methyl-D-erythritol-4-phosphate pathway in Saccharomyces cerevisiae.

    abstract::Transfer of a biosynthetic pathway between evolutionary distant organisms can create a metabolic shunt capable of bypassing the native regulation of the host organism, hereby improving the production of secondary metabolite precursor molecules for important natural products. Here, we report the engineering of Escheric...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-4877-y

    authors: Carlsen S,Ajikumar PK,Formenti LR,Zhou K,Phon TH,Nielsen ML,Lantz AE,Kielland-Brandt MC,Stephanopoulos G

    更新日期:2013-07-01 00:00:00

  • Directed evolution of an acid Yersinia mollaretii phytase for broadened activity at neutral pH.

    abstract::Phytases are phosphohydrolases that initiate the sequential hydrolysis of phosphate from phytate, which is the main storage form of phosphorous in numerous plant seeds, especially in cereals and grains. Phytate is indigestible for most monogastric animals, such as poultry, swine, fish, and humans; therefore, microbial...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-9308-7

    authors: Körfer G,Novoa C,Kern J,Balla E,Grütering C,Davari MD,Martinez R,Vojcic L,Schwaneberg U

    更新日期:2018-11-01 00:00:00

  • Screening of high-yielding biocontrol bacterium Bs-916 mutant by ion implantation.

    abstract::Bacillus subtilis 916 was an effective biocontrol agent in control rice sheath blight caused by Rhizoctonia solani. To further improve its antagonistic ability, low-energy ion implantation was applied in Bs-916. We studied the effects of different doses of N(+) implantation. The optimum dose of ion implantation for th...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-007-0951-7

    authors: Li D,Nie F,Wei L,Wei B,Chen Z

    更新日期:2007-07-01 00:00:00

  • Biochemical and molecular characterization of a thermostable chitosanase produced by the strain Paenibacillus sp. 1794 newly isolated from compost.

    abstract::Chitosan raises a great interest among biotechnologists due to its potential for applications in biomedical or environmental fields. Enzymatic hydrolysis of chitosan is a recognized method allowing control of its molecular size, making possible its optimization for a given application. During the industrial hydrolysis...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-012-4483-4

    authors: Zitouni M,Fortin M,Scheerle RK,Letzel T,Matteau D,Rodrigue S,Brzezinski R

    更新日期:2013-07-01 00:00:00

  • Analysis of genomic characters reveals that four distinct gene clusters are correlated with different functions in Burkholderia cenocepacia AU 1054.

    abstract::Possessing three circular chromosomes is a distinct genomic characteristic of Burkholderia cenocepacia AU 1054, a clinically important pathogen in cystic fibrosis. In this study, base composition, codon usage and functional role category were analyzed in the B. cenocepacia AU 1054 genome. Although no bias in the base ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-5415-7

    authors: Yuan J,Yang M,Ren J,Fu B,Jiang F,Zhang X

    更新日期:2014-01-01 00:00:00

  • In search of expression bottlenecks in recombinant CHO cell lines--a case study.

    abstract::The efficient production of recombinant proteins such as antibodies typically involves the screening of an extravagant number of clones in order to finally select a stable and high-producing cell line. Thereby, the underlying principles of a powerful protein machinery, but also potential expression limitations, often ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5584-z

    authors: Reinhart D,Sommeregger W,Debreczeny M,Gludovacz E,Kunert R

    更新日期:2014-07-01 00:00:00

  • Expression and characterization of a thermostable sarcosine oxidase (SOX) from Bacillus sp. in Escherichia coli.

    abstract::A heat-stable sarcosine oxidase produced by Bacillus sp. BSD-8 (SOX) had been studied and its complete gene sequence, which contained 1,164 bp nucleotides and encoded a protein of 387 amino acids, was obtained by DNA Walking method. The sox gene was cloned and functionally overexpressed in E. coli and the recombinant ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-006-0502-7

    authors: Guo K,Ma X,Sun G,Zhao Y,Li X,Zhao W,Kai L

    更新日期:2006-12-01 00:00:00

  • Changing glycine 21 for glutamic acid in the beta-subunit of penicillin G acylase from Kluyvera citrophila prevents protein maturation.

    abstract::Active penicillin acylase from Kluyvera citrophila strain ATCC 21,285 consists of two different alpha- and beta-subunits derived from a single precursor by post-translational processing. Using the chemical mutagen hydroxylamine we have treated plasmid pYKD59 containing the active penicillin acylase gene (pga) from K. ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00183245

    authors: Prieto I,Rodríguez MC,Márquez G,Pérez-Aranda A,Barbero JL

    更新日期:1992-02-01 00:00:00

  • Main lipophilic extractives in different paper pulp types can be removed using the laccase-mediator system.

    abstract::Lipophilic extractives in wood and other lignocellulosic materials exert a negative impact in pulp and paper manufacturing causing the so-called pitch problems. In this work, the appropriateness of an enzymatic treatment using the laccase-mediator system for pitch biocontrol is evaluated. With this purpose, three pulp...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-006-0346-1

    authors: Gutiérrez A,del Río JC,Rencoret J,Ibarra D,Martínez AT

    更新日期:2006-10-01 00:00:00

  • Release of ferulic acid from wheat bran by a ferulic acid esterase (FAE-III) from Aspergillus niger.

    abstract::Ferulic acid was efficiently released from a wheat bran preparation by a ferulic acid esterase from Aspergillus niger (FAE-III) when incubated together with a Trichoderma viride xylanase (a maximum of 95% total ferulic acid released after 5 h incubation). FAE-III by itself could release ferulic acid but at a level alm...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00166929

    authors: Faulds CB,Williamson G

    更新日期:1995-11-01 00:00:00

  • Induction, purification and characterization of alpha-N-acetylgalactosaminidase from Aspergillus Niger.

    abstract::A set of filamentous fungi (42 strains) was screened for alpha-N-acetylgalactosaminidase activity, and a series of inducers and different cultivation conditions were tested. Enzyme production by the best producer Aspergillus niger CCIM K2 was optimized and scaled up. alpha-N-Acetylgalactosaminidase was purified to app...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-008-1485-3

    authors: Weignerová L,Filipi T,Manglová D,Kren V

    更新日期:2008-07-01 00:00:00

  • Effects of sialylated lactulose on the mouse intestinal microbiome using Illumina high-throughput sequencing.

    abstract::Sialylated oligosaccharides are known to have beneficial effects, such as increasing the level of bifidobacteria, reducing the levels of blood endotoxin and blood ammonia, and enhancing the body's immune system. However, it is unknown whether sialylated lactuloses have modulatory effects on the intestinal microbiota. ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-019-10169-7

    authors: Song M,Zeng J,Jia T,Gao H,Zhang R,Jiang J,Li G,Su T

    更新日期:2019-11-01 00:00:00