Individual germinal centres of myasthenia gravis human thymuses contain polyclonal activated B cells that express all the Vh and Vk families.


:Using in situ hybridization, we analysed the immunoglobulin repertoire expressed by the B cells present in myasthenia gravis thymuses from four patients. B cells, mostly in activated state, were clustered in germinal centres, in which multiple isotypes were identified. A majority of cells expressed IgG as compared with IgM, with a roughly similar contribution of kappa and lambda chains. Hybridization with the six VH and the 4 VK human family probes was observed in serial sections, providing additional evidence that individual germinal centres were polyclonal. The thymic B cell repertoire closely reflected the VH and the VK family usage of normal peripheral blood lymphocytes with the preferential utilization of VH3, VK1 and VK3.


Clin Exp Immunol


Guigou V,Emilie D,Berrih-Aknin S,Fumoux F,Fougereau M,Schiff C




Has Abstract


1991-02-01 00:00:00












  • Natural killer cell activities of synbiotic Lactobacillus casei ssp. casei in conjunction with dextran.

    abstract::We have reported previously that Lactobacillus casei ssp. casei, together with specific substrate dextran, exhibited an adjuvant effect of stimulating humoral immune responses against bovine serum albumin (BSA) as a model antigen in BALB/c mice. In the present study, among the Lactobacillus species tested, L. casei ss...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Ogawa T,Asai Y,Tamai R,Makimura Y,Sakamoto H,Hashikawa S,Yasuda K

    更新日期:2006-01-01 00:00:00

  • Dynamics of T cells of L3T4 and Ly 2 phenotype within granulomas in murine listeriosis.

    abstract::Monoclonal antibodies anti-Ly 1, anti-Ly 2 and GK1.5 were applied to determine phenotypes of T cells within granulomas formed as a result of infection of mice with the facultative intracellular bacterium Listeria monocytogenes. Early in granuloma formation, equal numbers of Ly 1+, Ly 2+ and L3T4+ cells were found, T c...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Näher H,Sperling U,Takacs L,Hahn H

    更新日期:1985-06-01 00:00:00

  • Characterization of different types of experimental autoimmune thyroiditis in the Buffalo strain rat.

    abstract::We have compared features of experimental thyroiditis in the Buffalo strain rat induced by neonatal thymectomy, immunization with rat thyroglobulin (Tg) and complete Freund's adjuvant, and subcutaneous administration of trypan blue or 3-methylcholanthrene. The disease produced by neonatal thymectomy resulted in signif...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Cohen SB,Weetman AP

    更新日期:1987-07-01 00:00:00

  • Inhibition by glucocorticoid and staurosporine of IL-4-dependent CD23 production in B lymphocytes is reversed on engaging CD40.

    abstract::IL-4 synergizes with signals delivered through CD40 both for the induction of CD23/Fc epsilon RII expression and for IgE synthesis. Moreover, engagement of CD40 on the B cell surface by MoAb overcomes the ability of interferons, transforming growth factor-beta, or anti-CD19 to inhibit IL-4-dependent change. We now rep...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Katira A,Knox KA,Finney M,Michell RH,Wakelam M,Gordon J

    更新日期:1993-05-01 00:00:00

  • Infiltrating cellular pattern in kidney graft biopsies translates into forkhead box protein 3 up-regulation and p16INK4α senescence protein down-regulation in patients treated with belatacept compared to cyclosporin A.

    abstract::Renal allograft survival is related directly to cell senescence. In the transplantation scenario many cellular events - participating as immunological and non-immunological factors - could contribute to accelerate this biological process, responsible for the ultimate fate of the graft. Mechanisms concerned in toleranc...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Furuzawa-Carballeda J,Lima G,Alberú J,Palafox D,Uribe-Uribe N,Morales-Buenrostro LE,Reyes Acevedo R,Mondragón G,Chevaile A,Llorente L

    更新日期:2012-02-01 00:00:00

  • Label-free detection of immune complexes with myeloid cells.

    abstract::The aim of this study was to provide proof-of-concept for quantitative and qualitative label-free detection of immune complexes through myeloid cells with imaging surface plasmon resonance. Surface plasmon resonance imaging was first applied to monitor the binding of human sera from healthy and rheumatoid arthritis (R...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Szittner Z,Bentlage AE,Rovero P,Migliorini P,Lóránd V,Prechl J,Vidarsson G

    更新日期:2016-07-01 00:00:00

  • Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome.

    abstract::The efficacy of steroids and immunosuppressive treatments in idiopathic nephrotic syndrome (INS) hints at the implication of immune cells in the pathophysiology of the disease. Toll-like receptor (TLR) dysfunctions are involved in many kidney diseases of immune origin, but remain little described in INS. We investigat...

    journal_title:Clinical and experimental immunology

    pub_type: 临床试验,杂志文章


    authors: Jamin A,Dehoux L,Dossier C,Fila M,Heming N,Monteiro RC,Deschênes G

    更新日期:2015-12-01 00:00:00

  • Disruption of MAP kinase activation and nuclear factor binding to the IL-12 p40 promoter in HIV-infected myeloid cells.

    abstract::Progressive immunodeficiency in HIV infection is paralleled by a decrease in IL-12 production, a cytokine crucial for cellular immune function. Here we examine the molecular mechanisms by which HIV infection suppresses IL-12 p40 expression. HIV infection of THP-1 myeloid cells resulted in decreased LPS-induced nuclear...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Chambers KA,Parks RJ,Angel JB

    更新日期:2004-08-01 00:00:00

  • The influence of early life factors on the risk of developing rheumatoid arthritis.

    abstract::Rheumatoid arthritis (RA) is a chronic inflammatory disease that develops as a result of the interaction between genetic and environmental risk factors. Although increasing evidence shows the importance of genes in determining the risk of RA, it is clear that environmental factors also have a vital role. Studies to da...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Colebatch AN,Edwards CJ

    更新日期:2011-01-01 00:00:00

  • Subpopulations of thyroid autoantibody secreting lymphocytes in Graves' and Hashimoto thyroid glands.

    abstract::Lymphocytes isolated from Graves' and Hashimoto thyroid tissue by enzymatic (dispase) digestion or mechanical disaggregation were markedly different in terms of their ability to synthesize thyroid autoantibodies in culture. Dispase digestion, followed by removal of thyroid follicular cells, gave a lymphocyte populatio...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: McLachlan SM,Pegg CA,Atherton MC,Middleton SL,Dickinson A,Clark F,Proctor SJ,Proud G,Rees Smith B

    更新日期:1986-08-01 00:00:00

  • Surface activation markers of T lymphocytes: role in the detection of infection in neonates.

    abstract::Diagnosis of perinatal infection in the newborn is difficult; there may be few clinical signs and current tests are slow or non-specific. Detection of organisms, antigen or specific antibody to common pathogens often requires repeat samples and does not give immediate results. Haematological parameters, although relie...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Hodge S,Hodge G,Flower R,Han P

    更新日期:1998-07-01 00:00:00

  • An immunodominant La/SSB autoantibody proteome derives from public clonotypes.

    abstract::The La/SSB autoantigen is a major target of long-term humoral autoimmunity in primary Sjögren's Syndrome (SS) and systemic lupus erythematosus. A majority of patients with linked anti-Ro60/Ro52/La responses target an NH2-terminal epitope designated LaA that is expressed on Ro/La ribonucleoprotein complexes and the sur...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Thurgood LA,Arentz G,Lindop R,Jackson MW,Whyte AF,Colella AD,Chataway TK,Gordon TP

    更新日期:2013-11-01 00:00:00

  • Interference of PR3-ANCA with the enzymatic activity of PR3: differences in patients during active disease or remission of Wegener's granulomatosis.

    abstract::Anti-neutrophil cytoplasmic antibodies (ANCA) against proteinase 3 (PR3) are strongly associated with Wegener's granulomatosis (WG) and are thought to be involved in its pathogenesis. Levels of PR3-ANCA do not always correspond to clinical disease activity. To investigate the relationship between functional effects of...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: van der Geld YM,Tool AT,Videler J,de Haas M,Tervaert JW,Stegeman CA,Limburg PC,Kallenberg CG,Roos D

    更新日期:2002-09-01 00:00:00

  • Up-regulation of CCL11 and CCL26 is associated with activated eosinophils in bullous pemphigoid.

    abstract::Eosinophils contribute to the pathogenesis of bullous pemphigoid (BP) by secretion of proinflammatory cytokines and proteases. Trafficking of eosinophils into tissue in animal models and asthma depends on interleukin-5 and a family of chemokines named eotaxins, comprising CCL11, CCL24 and CCL26. Up-regulation of CCL11...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Günther C,Wozel G,Meurer M,Pfeiffer C

    更新日期:2011-11-01 00:00:00

  • Differential responses of human dendritic cells to metabolites from the oral/airway microbiome.

    abstract::Small molecule metabolites that are produced or altered by host-associated microbial communities are emerging as significant immune response modifiers. However, there is a key gap in our knowledge of how oral microbial metabolites affect the immune response. Here, we examined the effects of metabolites from five bacte...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Whiteson K,Agrawal S,Agrawal A

    更新日期:2017-06-01 00:00:00

  • Primary biliary cirrhosis: assessment of the quantitative importance of the adenine nucleotide translocator protein as a major mitochondrial antigen.

    abstract::The adenine nucleotide translocator protein (ANT) is the first well-characterized mitochondrial polypeptide to be identified as an antigen for antimitochondrial autoantibodies (AMA) in PBC sera. Because of the potential use for a highly purified antigen as a tool in studying the aetiology of PBC, we have undertaken an...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Mendel-Hartvig I,Frostell A,Nelson BD

    更新日期:1986-11-01 00:00:00

  • Specific stimulation of peripheral blood mononuclear cells from patients with acute myocarditis by peptide-bound flavin adenine dinucleotide (FAD), a naturally occurring autologous hapten.

    abstract::The tryptic FAD-peptide carrying the flavin in 8alpha-(N3)histidyl linkage as natural hapten was isolated by HPLC from the bacterial enzyme 6-hydroxy-d-nicotine oxidase. The same flavin protein linkage is found in the mitochondrial succinate dehydrogenase flavoprotein subunit, the predominant flavoprotein with covalen...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Cicek G,Schiltz E,Staiger J,Neumann FJ,Melchers I,Brandsch R

    更新日期:2003-05-01 00:00:00

  • CD4+ CD25+ transforming growth factor-beta-producing T cells are present in the lung in murine tuberculosis and may regulate the host inflammatory response.

    abstract::CD4(+) CD25(+) regulatory T cells produce the anti-inflammatory cytokines transforming growth factor (TGF)-beta or interleukin (IL)-10. Regulatory T cells have been recognized to suppress autoimmunity and promote self-tolerance. These cells may also facilitate pathogen persistence by down-regulating the host defence r...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Mason CM,Porretta E,Zhang P,Nelson S

    更新日期:2007-06-01 00:00:00

  • Evidence for anti-tubulin autoantibodies in the form of immune complexes in human sera.

    abstract::Anti-tubulin antibodies were studied in normal human serum either maintained at neutral pH to measure the free antibody activity (FAA) or treated at pH 2.8 to measure the total antibody activity (TAA): FAA + the antibody activity in the form of immune complexes (ICAA). Anti-tubulin antibody activities were assessed by...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Bernier-Valentin F,Rabilloud R,Rousset B

    更新日期:1988-02-01 00:00:00

  • Production and secretion of interferon-gamma (IFN-gamma) in children with atopic dermatitis.

    abstract::IFN-gamma is known to be a major inhibitor of IgE synthesis in vitro. Recent studies demonstrating reduced production of IFN-gamma in children and adults with atopic dermatitis and elevated serum IgE suggest a similar role for this cytokine in vivo. The reasons for this reduced IFN-gamma production are not known. One ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Tang M,Kemp A

    更新日期:1994-01-01 00:00:00

  • In vivo tumouricidal effects of LAD-1 monoclonal antibody on murine RL-male-1 lymphoma mediated by enhanced phagocytosis.

    abstract::We have reported previously that the LAD-4 monoclonal antibody (mAb) directed against a fibronectin receptor (FNR) on RL-male-1 T lymphoma cells in BALB/c mice partially inhibited their migration to the liver. In the present study, we examined the mechanism by which another anti-FNR mAb, LAD-1, exerts its antitumourig...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Ito M,Omoto S,Kato Y,Hayashi T,Mori N,Fujii YR

    更新日期:2005-07-01 00:00:00

  • Precipitable immune complexes in healthy homosexual men, acquired immune deficiency syndrome and the related lymphadenopathy syndrome.

    abstract::Increased levels of 3% PEG precipitable circulating immune complexes (CIC) were found in healthy homosexual men, in homosexual patients with the acquired immune deficiency syndrome (AIDS), and in the AIDS related lymphadenopathy syndrome (LAS). The degree of CIC elevation increases from healthy homosexual men to LAS a...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Euler HH,Kern P,Löffler H,Dietrich M

    更新日期:1985-02-01 00:00:00

  • Antibody to severe acute respiratory syndrome (SARS)-associated coronavirus spike protein domain 2 cross-reacts with lung epithelial cells and causes cytotoxicity.

    abstract::Both viral effect and immune-mediated mechanism are involved in the pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. In this study, we showed that in SARS patient sera there were autoantibodies (autoAbs) that reacted with A549 cells, the type-2 pneumocytes, and that these ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Lin YS,Lin CF,Fang YT,Kuo YM,Liao PC,Yeh TM,Hwa KY,Shieh CC,Yen JH,Wang HJ,Su IJ,Lei HY

    更新日期:2005-09-01 00:00:00

  • Immunosuppression by sex steroid hormones. The effect upon PHA- and PPD-stimulated lymphocytes.

    abstract::Progesterone, estradiol, testosterone, cortisol, and 11-desoxycortisol (compound S) were added to cultures of human lymphocytes stimulated with phytohaemagglutinin (PHA) and purified protein derivative (PPD). The immunosuppressive effect of cortisol was verified and the three sex-steroid hormones also were found to in...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Wyle FA,Kent JR

    更新日期:1977-03-01 00:00:00

  • C3 nephritic factor and hypocomplementaemia in a clinically healthy individual.

    abstract::Nephritic factor was detected in an individual whose serum showed a selective decrease in the third component of complement (C3) during and subsequent to an illness resembling disseminated gonococcal infection (DGI). As is characteristic of C3 nephritic factor (C3NeF), its activity was heat stable, was associated with...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Gewurz AT,Imherr SM,Strauss S,Gewurz H,Mold C

    更新日期:1983-10-01 00:00:00

  • Autoantibodies recognizing citrullinated rat filaggrin in an ELISA using citrullinated and non-citrullinated recombinant proteins as antigens are highly diagnostic for rheumatoid arthritis.

    abstract::The objective of the study was to determine the diagnostic value for rheumatoid arthritis (RA) of anti-filaggrin autoantibodies (autoAb) recognizing citrullinated recombinant rat filaggrin (ACRF) in community cases of very early arthritis. To evaluate the diagnostic value of ACRF, were studied sera from patients with ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,多中心研究


    authors: Vittecoq O,Incaurgarat B,Jouen-Beades F,Legoedec J,Letourneur O,Rolland D,Gervasi G,Ménard JF,Gayet A,Fardellone P,Daragon A,Jolivet M,le Loët X,Tron F

    更新日期:2004-01-01 00:00:00

  • A novel point mutation in CD18 causing the expression of dysfunctional CD11/CD18 leucocyte integrins in a patient with leucocyte adhesion deficiency (LAD).

    abstract::Leucocyte adhesion deficiency type 1 (LAD-1) is characterized by the incapacity of leucocytes to carry out their adhesion functions via their CD11/CD18 antigens, which are also referred to as the leucocyte integrins. The patients generally suffer from poor wound healing and recurrent bacterial and fungal infections. I...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Mathew EC,Shaw JM,Bonilla FA,Law SK,Wright DA

    更新日期:2000-07-01 00:00:00

  • Antibody-dependent cellular cytotoxicity in recurrent aphthous ulceration.

    abstract::Antibody-dependent cellular cytotoxicity (ADCC) was investigated as s possible mechanism of pathogenesis in recurrent aphthous ulceration (RAU). ADCC induced by mononuclear cells from patients at various stages of RAU was compared with ADCC induced by mononuclear cells from paired controls. Chicken red blood cells (Ch...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Greenspan JS,Gadol N,Olson JA,Talal N

    更新日期:1981-06-01 00:00:00

  • Autoantibodies to intermediate filaments in sera of patients with Schistosoma mansoni infection.

    abstract::Autoantibodies to the intermediate filament proteins vimentin and keratin were studied in sera of 50 Caribbean patients with Schistosoma mansoni infection and 50 control subjects. Autoantibodies were detected by indirect immunofluorescence on HEp-2 cells pretreated with colchicine. The incidence of anti-vimentin antib...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Boehme MW,Kataaha PK,Holborow EJ

    更新日期:1989-08-01 00:00:00

  • Whole blood stimulation with Toll-like receptor (TLR)-7/8 and TLR-9 agonists induces interleukin-12p40 expression in plasmacytoid dendritic cells in rhesus macaques but not in humans.

    abstract::Macaques provide important animal models in biomedical research into infectious and chronic inflammatory disease. Therefore, a proper understanding of the similarities and differences in immune function between macaques and humans is needed for adequate interpretation of the data and translation to the human situation...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Koopman G,Beenhakker N,Burm S,Bouwhuis O,Bajramovic J,Sommandas V,Mudde G,Mooij P,'t Hart BA,Bogers WM

    更新日期:2013-10-01 00:00:00