An in-depth analysis of the biological functional studies based on the NMR M2 channel structure of influenza A virus.

Abstract:

:The long-sought three-dimensional structure of the M2 proton channel of influenza A virus was successfully determined recently by the high-resolution NMR [J.R. Schnell, J.J. Chou, Structure and mechanism of the M2 proton channel of influenza A virus, Nature 451 (2008) 591-595]. Such a milestone work has provided a solid structural basis for studying drug-resistance problems. However, the action mechanism revealed from the NMR structure is completely different from the traditional view and hence prone to be misinterpreted as "conflicting" with some previous biological functional studies. To clarify this kind of confusion, an in-depth analysis was performed for these functional studies, particularly for the mutations D44N, D44A and N44D on position 44, and the mutations on positions 27-38. The analyzed results have provided not only compelling evidences to further validate the NMR structure but also very useful clues for dealing with the drug-resistance problems and developing new effective drugs against H5N1 avian influenza virus, an impending threat to human beings.

authors

Huang RB,Du QS,Wang CH,Chou KC

doi

10.1016/j.bbrc.2008.10.148

subject

Has Abstract

pub_date

2008-12-26 00:00:00

pages

1243-7

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(08)02135-9

journal_volume

377

pub_type

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