Identification of IGFBP-6 as an effector of the tumor suppressor activity of SEMA3B.


:SEMA3B, a member of class 3 semaphorins, is a tumor suppressor. Competition with vascular endothelial growth factor (VEGF)165 explains a portion of the activity, whereas the VEGF-independent mechanism was not elucidated. We employed a microarray and screened for the genes whose expression was increased by SEMA3B in NCI-H1299 cells. Insulin-like growth factor-binding protein-6 (IGFBP-6), a tumor suppressor, showed greatest difference in the expression level. Introduction of IGFBP-6 cDNA reduced colony formation both on the dish surface and in soft agar. Insulin-like growth factor II, which antagonizes IGFBP-6, partly abrogated the effect. Inhibition of IGFBP-6 by small interfering RNA diminished the sub-G0/G1 population that was induced by SEMA3B and abrogated the growth suppressive effect of SEMA3B. We concluded that IGFBP-6 is the effector of tumor suppressor activity of SEMA3B in NCI-H1299 cells. It has been reported that beta-catenin suppresses the expression of IGFBP-6. Introduction of beta-catenin into the cells partly abrogated the growth suppressive effect of SEMA3B. Our result indicates that semaphorin signaling and beta-catenin signaling converge on IGFBP-6 and antithetically affect their functions.






Koyama N,Zhang J,Huqun,Miyazawa H,Tanaka T,Su X,Hagiwara K




Has Abstract


2008-11-20 00:00:00














  • Prostate stem cell antigen (PSCA) expression increases with high gleason score, advanced stage and bone metastasis in prostate cancer.

    abstract::Prostate stem cell antigen (PSCA) is a recently defined homologue of the Thy-1/Ly-6 family of glycosylphosphatidylinositol (GPI)-anchored cell surface antigens. PSCA mRNA is expressed in the basal cells of normal prostate and in more than 80% of prostate cancers. The purpose of the present study was to examine PSCA pr...


    pub_type: 杂志文章


    authors: Gu Z,Thomas G,Yamashiro J,Shintaku IP,Dorey F,Raitano A,Witte ON,Said JW,Loda M,Reiter RE

    更新日期:2000-03-02 00:00:00

  • Alternatively spliced HPV-18 E6* protein inhibits E6 mediated degradation of p53 and suppresses transformed cell growth.

    abstract::The E6 proteins originating from the tumour-associated Human Papillomavirus (HPV) types 16 and 18 have been shown to bind to and target the tumour suppressor protein, p53, for ubiquitin-mediated degradation. However, in cell lines derived from cervical neoplasias, the predominant early region transcripts are spliced a...


    pub_type: 杂志文章


    authors: Pim D,Massimi P,Banks L

    更新日期:1997-07-17 00:00:00

  • WAVE3, an actin-polymerization gene, is truncated and inactivated as a result of a constitutional t(1;13)(q21;q12) chromosome translocation in a patient with ganglioneuroblastoma.

    abstract::Neuroblastoma (Nb) is a malignancy of the sympathetic nervous system which affects children in their first decade. It is the most common extra-cranial solid tumor in children with an incidence of approximately 1 in 8-10 000 live births annually and accounts for approximately 10% of all children's cancers. Ganglioneuro...


    pub_type: 杂志文章


    authors: Sossey-Alaoui K,Su G,Malaj E,Roe B,Cowell JK

    更新日期:2002-08-29 00:00:00

  • Dexamethasone induces apoptosis of multiple myeloma cells in a JNK/SAP kinase independent mechanism.

    abstract::The stress-activated protein kinases (SAPKs), also known as c-Jun amino-terminal kinases (JNKs), are activated in response to diverse stimuli including DNA damage, heat shock, interleukin-1, tumor necrosis factor-alpha and Fas. Although all these inducers cause apoptosis, whether SAPK/JNK activation is required for ap...


    pub_type: 杂志文章


    authors: Chauhan D,Pandey P,Ogata A,Teoh G,Treon S,Urashima M,Kharbanda S,Anderson KC

    更新日期:1997-08-14 00:00:00

  • The mismatch DNA repair heterodimer, hMSH2/6, regulates BLM helicase.

    abstract::The human MSH2/6 complex is essential for mismatch recognition during the repair of replication errors. Although mismatch repair components have been implicated in DNA homologous recombination repair, the exact function of hMSH2/6 in this pathway is unclear. Here, we show that the recombinant hMSH2/6 protein complex s...


    pub_type: 杂志文章


    authors: Yang Q,Zhang R,Wang XW,Linke SP,Sengupta S,Hickson ID,Pedrazzi G,Perrera C,Stagljar I,Littman SJ,Modrich P,Harris CC

    更新日期:2004-05-06 00:00:00

  • Characterization of changes in gene expression associated with malignant transformation by the NF-kappaB family member, v-Rel.

    abstract::In this study, alterations in gene expression patterns have been examined in v-Rel-transformed avian bone marrow cells. Using a conditional v-Rel estrogen receptor chimera (v-RelER) which transforms cells in an estrogen-dependent manner, we constructed subtraction cDNA libraries from v-RelER-transformed bone marrow ce...


    pub_type: 杂志文章


    authors: Petrenko O,Ischenko I,Enrietto PJ

    更新日期:1997-10-02 00:00:00

  • OTUB1 inhibits the ubiquitination and degradation of FOXM1 in breast cancer and epirubicin resistance.

    abstract::The forkhead transcription factor FOXM1 has a key role in DNA damage response, and its deregulated overexpression is associated with genotoxic drug resistance in breast cancer. However, little is known about the posttranslational mechanisms by which FOXM1 expression is regulated by genotoxic agents and how they are de...


    pub_type: 杂志文章


    authors: Karunarathna U,Kongsema M,Zona S,Gong C,Cabrera E,Gomes AR,Man EP,Khongkow P,Tsang JW,Khoo US,Medema RH,Freire R,Lam EW

    更新日期:2016-03-17 00:00:00

  • The adenovirus E1A 289R and 243R proteins inhibit the phosphorylation of p300.

    abstract::A protein of 300 kDa (p300) associates with the adenovirus E1A proteins and has been implicated in the control of cell cycle progression. In mammalian cells, p300 is actively phosphorylated in both quiescent and proliferating cells and its level of phosphorylation increases as it travels from late G1 into M phase. E1A...


    pub_type: 杂志文章


    authors: Banerjee AC,Recupero AJ,Mal A,Piotrkowski AM,Wang DM,Harter ML

    更新日期:1994-06-01 00:00:00

  • A novel germline 1.8-kb deletion of hMLH1 mimicking alternative splicing: a founder mutation in the Chinese population.

    abstract::We have previously reported that there is a high incidence of microsatellite instability (MSI) and germline mismatch repair gene mutation in colorectal cancer arising from young Hong Kong Chinese. Most of the germline mutations involve hMSH2, which is different from the mutation spectrum in the Western population. It ...


    pub_type: 杂志文章


    authors: Chan TL,Yuen ST,Ho JW,Chan AS,Kwan K,Chung LP,Lam PW,Tse CW,Leung SY

    更新日期:2001-05-24 00:00:00

  • Development of cellular resistance to pp60v-src kinase-induced cell death.

    abstract::The v-src gene of Rous sarcoma virus (RSV) encodes pp60v-src, a tyrosine kinase that can initiate cellular transformation. High levels of v-src gene expression can either be cytotoxic or the cause of altered expression of cellular genes. Examination of cytotoxic thresholds is difficult because cells expressing high le...


    pub_type: 杂志文章


    authors: Wu LW,Hackett PB

    更新日期:1995-10-19 00:00:00

  • Butyrolactone I, a selective inhibitor of cdk2 and cdc2 kinase.

    abstract::We screened cdc2 kinase inhibitors from cultured mediums of micro organisms using purified mouse cyclin B-cdc2 kinase and a specific substrate peptide for cdc2 kinase. A selective inhibitor of cdc2 kinase was isolated from the cultured medium of Aspergillus species F-25799, and identified as butyrolactone I. Butyrolac...


    pub_type: 杂志文章


    authors: Kitagawa M,Okabe T,Ogino H,Matsumoto H,Suzuki-Takahashi I,Kokubo T,Higashi H,Saitoh S,Taya Y,Yasuda H

    更新日期:1993-09-01 00:00:00

  • Characterization of a c-met proto-oncogene activated in human xeroderma pigmentosum cells after treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).

    abstract::Human xeroderma pigmentosum (XP) fibroblasts were transformed with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The transformed cells, called ASKMN, were immortalized, grew in agar and were tumorigenic in nude mice. A trp-met oncogene was identified in ASKMN cells, after transfection of high molecular weight DNA on 3T...


    pub_type: 杂志文章


    authors: Michelin S,Daya-Grosjean L,Sureau F,Saïd S,Sarasin A,Suárez HG

    更新日期:1993-07-01 00:00:00

  • Regulation of NF-kappaB activity by I kappaB-related proteins in adenocarcinoma cells.

    abstract::Constitutive NF-kappaB activity varies widely among cancer cell lines. In this report, we studied the expression and the role of different I kappaB inhibitors in adenocarcinoma cell lines. High constitutive NF-kappaB activity and low I kappaB-alpha expression was found in a number of these cell lines. Moreover, some o...


    pub_type: 杂志文章


    authors: Dejardin E,Deregowski V,Chapelier M,Jacobs N,Gielen J,Merville MP,Bours V

    更新日期:1999-04-22 00:00:00

  • Differential effects of the widely expressed dMax splice variant of Max on E-box vs initiator element-mediated regulation by c-Myc.

    abstract::dMax, a naturally occurring splice variant of the Myc binding protein Max, lacks the DNA binding basic region and helix 1 of the Helix-Loop-Helix domain; dMax interacts with c-Myc in vitro and in vivo, and inhibits E-box Myc site driven transcription in transient transfection assays. Here we have investigated the expr...


    pub_type: 杂志文章


    authors: FitzGerald MJ,Arsura M,Bellas RE,Yang W,Wu M,Chin L,Mann KK,DePinho RA,Sonenshein GE

    更新日期:1999-04-15 00:00:00

  • Activation of the hedgehog pathway confers a poor prognosis in embryonal and fusion gene-negative alveolar rhabdomyosarcoma.

    abstract::Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children and comprises two major histological subtypes: alveolar rhabdomyosarcoma (ARMS) and embryonal rhabdomyosarcoma (ERMS). Seventy-five percent of ARMS harbor reciprocal chromosomal translocations leading to fusion genes of the forkhead transcriptio...


    pub_type: 杂志文章


    authors: Zibat A,Missiaglia E,Rosenberger A,Pritchard-Jones K,Shipley J,Hahn H,Fulda S

    更新日期:2010-12-02 00:00:00

  • Ras-independent activation of Rel-family transcription factors by UVB and TPA in cultured keratinocytes.

    abstract::UV irradiation of mammalian cells results in the activation of transcription factors which mediate induction of early response genes designed to repair and minimise the damage sustained by the cell. Evidence from studies in HeLa cells suggest that UVC regulates NF-kappa B activity via tyrosine kinases and activation o...


    pub_type: 杂志文章


    authors: Tobin D,Nilsson M,Toftgård R

    更新日期:1996-02-15 00:00:00

  • Signal transduction pathways regulated by arsenate and arsenite.

    abstract::Arsenate and arsenite activate c-Jun N-terminal kinase (JNK), however, the mechanism by which this occurs is not known. By expressing inhibitory mutant small GTP-binding proteins, p21-activated kinase (PAK) and mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinases (MEKKs), we have ident...


    pub_type: 杂志文章


    authors: Porter AC,Fanger GR,Vaillancourt RR

    更新日期:1999-12-16 00:00:00

  • Cytokine control of developmental programs in normal hematopoiesis and leukemia.

    abstract::The establishment of a system for in vitro clonal development of hematopoietic cells made it possible to discover the cytokines that regulate hematopoiesis. These cytokines include colony stimulating factors and others, which interact in a network, and there is a cytokine cascade which couples growth and differentiati...


    pub_type: 杂志文章,评审


    authors: Lotem J,Sachs L

    更新日期:2002-05-13 00:00:00

  • Interaction with PI3-kinase contributes to the cytotoxic activity of apoptin.

    abstract::Apoptin, a small protein from the chicken anemia virus, has attracted attention because of its specificity in killing tumor cells. Localization of apoptin in the nucleus of tumor cells has been shown to be vital for proapoptotic activity, however, targeted expression of apoptin in the nucleus of normal cells does not ...


    pub_type: 杂志文章


    authors: Maddika S,Wiechec E,Ande SR,Poon IK,Fischer U,Wesselborg S,Jans DA,Schulze-Osthoff K,Los M

    更新日期:2008-05-08 00:00:00

  • Wild-type p53 gene transfer is not detrimental to normal cells in vivo: implications for tumor gene therapy.

    abstract::The p53 oncosuppressor is strictly maintained in an inactive form under normal conditions, while it is post-translationally activated by a variety of stresses, enacting different protective biological functions. Since one critical issue in cancer gene therapy is tumor specificity, we asked whether the tight p53 regula...


    pub_type: 杂志文章


    authors: Bossi G,Mazzaro G,Porrello A,Crescenzi M,Soddu S,Sacchi A

    更新日期:2004-01-15 00:00:00

  • Expression of alpha-catenin in alpha-catenin-deficient cells results in a reduced proliferation in three-dimensional multicellular spheroids but not in two-dimensional monolayer cultures.

    abstract::alpha-Catenin is an intracellular protein that associates with the carboxy-terminal region of cadherin, a cell adhesion molecule, via beta-catenin or gamma-catenin (plakoglobin). Linkage of cadherin to the cytoskeleton by catenins is required for full cadherin activity. Following transfection of an alpha-catenin-defic...


    pub_type: 杂志文章


    authors: Matsubara S,Ozawa M

    更新日期:2004-04-08 00:00:00

  • A kinome-wide shRNA screen uncovers vaccinia-related kinase 3 (VRK3) as an essential gene for diffuse intrinsic pontine glioma survival.

    abstract::Diffuse intrinsic pontine glioma (or DIPG) are pediatric high-grade gliomas associated with a dismal prognosis. They harbor specific substitution in histone H3 at position K27 that induces major epigenetic dysregulations. Most clinical trials failed so far to increase survival, and radiotherapy remains the most effici...


    pub_type: 杂志文章


    authors: Silva-Evangelista C,Barret E,Ménez V,Merlevede J,Kergrohen T,Saccasyn A,Oberlin E,Puget S,Beccaria K,Grill J,Castel D,Debily MA

    更新日期:2019-09-01 00:00:00

  • Post-translational regulation of PTEN.

    abstract::PTEN (phosphatase and tensin homolog deleted on chromosome 10) tumor suppressor regulates a variety of cellular processes including cell proliferation, growth, migration and death. This master regulator itself is also under deliberative regulation. Although the evidence for PTEN regulation and its significance in norm...


    pub_type: 杂志文章,评审


    authors: Wang X,Jiang X

    更新日期:2008-09-18 00:00:00

  • The renin angiotensin system (RAS) mediates bifunctional growth regulation in melanoma and is a novel target for therapeutic intervention.

    abstract::Despite emergence of new systemic therapies, metastatic melanoma remains a challenging and often fatal form of skin cancer. The renin-angiotensin system (RAS) is a major physiological regulatory pathway controlling salt-water equilibrium, intravascular volume and blood pressure. Biological effects of the RAS are media...


    pub_type: 杂志文章


    authors: Renziehausen A,Wang H,Rao B,Weir L,Nigro CL,Lattanzio L,Merlano M,Vega-Rioja A,Del Carmen Fernandez-Carranco M,Hajji N,Matin R,Harwood C,Li S,Sim VR,O'Neill K,Evans A,Thompson A,Szlosarek P,Fleming C,Stebbing J,Pr

    更新日期:2019-03-01 00:00:00

  • Cell growth-regulated expression of mammalian MCM5 and MCM6 genes mediated by the transcription factor E2F.

    abstract::Initiation of DNA replication requires the function of MCM gene products, which participate in ensuring that DNA replication occurs only once in the cell cycle. Expression of all mammalian genes of the MCM family is induced by growth stimulation, unlike yeast, and the mRNA levels peak at G1/S boundary. In this study, ...


    pub_type: 杂志文章


    authors: Ohtani K,Iwanaga R,Nakamura M,Ikeda M,Yabuta N,Tsuruga H,Nojima H

    更新日期:1999-04-08 00:00:00

  • Characterization of Wnt-1 and Wnt-2 induced growth alterations and signaling pathways in NIH3T3 fibroblasts.

    abstract::Members of the Wnt family induce mouse mammary tumors and partially transform mammary epithelial cells in culture. However, their mechanism of transformation remains to be elucidated. In NIH3T3 mouse embryo fibroblasts, a standard transformation model, Wnt-1 and Wnt-2 were shown to induce altered properties including ...


    pub_type: 杂志文章


    authors: Bafico A,Gazit A,Wu-Morgan SS,Yaniv A,Aaronson SA

    更新日期:1998-05-28 00:00:00

  • Cyclin D1 integrates G9a-mediated histone methylation.

    abstract::Lysine methylation of histones and non-histone substrates by the SET domain containing protein lysine methyltransferase (KMT) G9a/EHMT2 governs transcription contributing to apoptosis, aberrant cell growth, and pluripotency. The positioning of chromosomes within the nuclear three-dimensional space involves interaction...


    pub_type: 杂志文章


    authors: Li Z,Jiao X,Di Sante G,Ertel A,Casimiro MC,Wang M,Katiyar S,Ju X,Klopfenstein DV,Tozeren A,Dampier W,Chepelev I,Jeltsch A,Pestell RG

    更新日期:2019-05-01 00:00:00

  • Cancer whole-genome sequencing: present and future.

    abstract::Recent explosive advances in next-generation sequencing technology and computational approaches to massive data enable us to analyze a number of cancer genome profiles by whole-genome sequencing (WGS). To explore cancer genomic alterations and their diversity comprehensively, global and local cancer genome-sequencing ...


    pub_type: 杂志文章,评审


    authors: Nakagawa H,Wardell CP,Furuta M,Taniguchi H,Fujimoto A

    更新日期:2015-12-03 00:00:00

  • Developmental regulation of intermediate filament and actin mRNAs during myogenesis is disrupted by oncogenic ras genes.

    abstract::Terminal differentiation of skeletal myoblasts is accompanied by down-regulation of vimentin and beta-, gamma-actins and up-regulation of desmin and sarcomeric alpha-actin(s). To investigate whether the normal decline in expression of vimentin and beta-, gamma-actins was coupled to withdrawal of proliferating myoblast...


    pub_type: 杂志文章


    authors: Olson EN,Capetanaki YG

    更新日期:1989-07-01 00:00:00

  • Non-canonical activation of hedgehog in prostate cancer cells mediated by the interaction of transcriptionally active androgen receptor proteins with Gli3.

    abstract::Hedgehog (Hh) is an oncogenic signaling pathway that regulates the activity of Gli transcription factors. Canonical Hh is a Smoothened- (Smo-) driven process that alters the post-translational processing of Gli2/Gli3 proteins. Though evidence supports a role for Gli action in prostate cancer (PCa) cell growth and prog...


    pub_type: 杂志文章


    authors: Li N,Truong S,Nouri M,Moore J,Al Nakouzi N,Lubik AA,Buttyan R

    更新日期:2018-04-01 00:00:00