Chronic hyperhomocysteinemia alters antioxidant defenses and increases DNA damage in brain and blood of rats: protective effect of folic acid.


:We have previously demonstrated that acute hyperhomocysteinemia induces oxidative stress in rat brain. In the present study, we initially investigated the effect of chronic hyperhomocysteinemia on some parameters of oxidative damage, namely total radical-trapping antioxidant potential and activities of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase), as well as on DNA damage in parietal cortex and blood of rats. We also evaluated the effect of folic acid on biochemical alterations elicited by hyperhomocysteinemia. Wistar rats received daily subcutaneous injection of Hcy (0.3-0.6 micromol/g body weight), and/or folic acid (0.011 micromol/g body weight) from their 6th to their 28th day of life. Twelve hours after the last injection the rats were sacrificed, parietal cortex and total blood was collected. Results showed that chronic homocysteine administration increased DNA damage, evaluated by comet assay, and disrupted antioxidant defenses (enzymatic and non-enzymatic) in parietal cortex and blood/plasma. Folic acid concurrent administration prevented homocysteine effects, possibly by its antioxidant and DNA stability maintenance properties. If confirmed in human beings, our results could propose that the supplementation of folic acid can be used as an adjuvant therapy in disorders that accumulate homocysteine.


Neurochem Int


Matté C,Mackedanz V,Stefanello FM,Scherer EB,Andreazza AC,Zanotto C,Moro AM,Garcia SC,Gonçalves CA,Erdtmann B,Salvador M,Wyse AT




Has Abstract


2009-01-01 00:00:00














  • External pH changes affect NMDA-evoked and spontaneous release of cholecystokinin, somatostatin and noradrenaline from rat cerebrocortical nerve endings.

    abstract::It was previously reported that the K+-evoked release of somatostatin-like immunoreactivity (SRIF-LI) and of cholecystokinin-like immunoreactivity (CCK-LI) from superfused rat cerebrocortical synaptosomes can be enhanced by NMDA or D-serine alone. We here studied the effects of extraterminal pH changes on SRIF-LI and ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Gemignani A,Paudice P,Longordo F,Raiteri M

    更新日期:2004-10-01 00:00:00

  • Stimulation of myelin proteolipid protein gene expression by eicosapentaenoic acid in C6 glioma cells.

    abstract::In this study, the role of exogenous fatty acids in the regulation of proteolipid protein (PLP) gene expression was investigated using the following model culture system: C6 glioma cells expressing the green-fluorescent protein (eGFP) driven by different segments of PLP promoter. Eicosapentanoic acid (EPA; 20:5 n-3), ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Salvati S,Natali F,Attorri L,Raggi C,Di Biase A,Sanchez M

    更新日期:2004-04-01 00:00:00

  • Effects of lithium, alone or associated with pilocarpine, on muscarinic and dopaminergic receptors and on phosphoinositide metabolism in rat hippocampus and striatum.

    abstract::The mechanism of action of lithium (Li) alone or with pilocarpine (Pilo), focusing on muscarinic and dopaminergic systems and also on phosphoinositide metabolism was studied. Li (3 mEq/kg) administered to rats once (1 d) or daily for 7 days (7 d), 24 h before Pilo (15 mg/kg), exacerbated cholinergic signs, leading to ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Marinho MM,de Sousa FC,de Bruin VM,Vale MR,Viana GS

    更新日期:1998-10-01 00:00:00

  • Methamphetamine use causes cognitive impairment and altered decision-making.

    abstract::Methamphetamine is a widely abused psychostimulant. It reverses transport through the dopamine transporter, thereby increasing the extracellular level of dopamine in the brain, which is associated with the rewarding effect. Repeated intake of methamphetamine leads to drug addiction, a chronically relapsing disorder ch...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审


    authors: Mizoguchi H,Yamada K

    更新日期:2019-03-01 00:00:00

  • Short-term effect of stress on tyrosine hydroxylase activity.

    abstract::The short-term influences of stress on the activities of tyrosine hydroxylase in vivo and in vitro were examined in mice. The in vivo tyrosine hydroxylase activity was estimated by the rate of dopa accumulation which was measured at 30 min after the injection of NSD-1015 (100 mg kg), an aromatic l-amino acid decarboxy...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Oka K,Ashiba G,Kiss B,Nagatsu T

    更新日期:1982-01-01 00:00:00

  • Sodium thiosulfate protects brain in rat model of adenine induced vascular calcification.

    abstract::Vascular bed calcification is a common feature of ends stage renal disease that may lead to a complication in cardiovascular and cerebrovascular beds, which is a promoting cause of myocardial infarction, stroke, dementia and aneurysms. Sodium thiosulfate (STS) due to its multiple properties such as antioxidant and cal...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Subhash N,Sriram R,Kurian GA

    更新日期:2015-11-01 00:00:00

  • Free choline and choline metabolites in rat brain and body fluids: sensitive determination and implications for choline supply to the brain.

    abstract::In the central nervous system, choline is an essential precursor of choline-containing phospholipids in neurons and glial cells and of acetylcholine in cholinergic neurons. In order to study choline transport and metabolism in the brain, we developed a comprehensive methodical procedure for the analysis of choline and...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Klein J,Gonzalez R,Köppen A,Löffelholz K

    更新日期:1993-03-01 00:00:00

  • Unchanged glutamine synthetase activity and increased NMDA receptor density in epileptic human neocortex: implications for the pathophysiology of epilepsy.

    abstract::We investigated whether alterations in glutamate metabolising glutamine synthetase activity occur in human epileptic neocortex, as shown previously for human epileptic hippocampus [Eid, T., Thomas, M.J., Spencer, D.D., Rundén-Pran, E., Lai, J.C.K., Malthankar, G.V., Kim, J.H., Danbolt, N.C., Ottersen, O.P., de Lanerol...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Steffens M,Huppertz HJ,Zentner J,Chauzit E,Feuerstein TJ

    更新日期:2005-11-01 00:00:00

  • Partial purification of ?-glycerotoxin, a presynaptic neurotoxin from the venom glands of the polychaete annelid glycera convoluta.

    abstract::The venom secreted from glands appended to the jaws of Glycera convoluta, a Polychaete Annelid, increases the spontaneous quantal release of transmitter from nerve terminals. The component that is biologically active on vertebrate cholinergic nerve terminals has recently been shown to be a high molecular weight protei...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Bon C,Saliou B,Thieffry M,Manaranche R

    更新日期:1985-01-01 00:00:00

  • Intracellular disulfide reduction by phosphine-borane complexes: Mechanism of action for neuroprotection.

    abstract::Phosphine-borane complexes are novel cell-permeable drugs that protect neurons from axonal injury in vitro and in vivo. These drugs activate the extracellular signal-regulated kinases 1/2 (ERK1/2) cell survival pathway and are therefore neuroprotective, but do not scavenge superoxide. In order to understand the intera...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Niemuth NJ,Thompson AF,Crowe ME,Lieven CJ,Levin LA

    更新日期:2016-10-01 00:00:00

  • Differential effect of platelet activating factor on 1-methyl-4-phenylpyridinium-induced cell death through regulation of apoptosis-related protein activation.

    abstract::Platelet activating factor (PAF) has been suggested to play a critical role in the pathogenesis of neurological disorders. We assessed the effect of PAF against the toxicity of 1-methyl-4-phenylpyridinium (MPP(+)), a parkinsonian toxin, in relation to apoptotic process. PAF exhibited differential effect against the MP...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Ha SW,Lee CS

    更新日期:2010-05-01 00:00:00

  • K(+)-evoked depolarization induces serotonin N-acetyltransferase activity in photoreceptor-enriched retinal cell cultures. Involvement of calcium influx through l-type channels.

    abstract::The roles of membrane depolarization and calcium influx in the regulation of retinal serotonin N-acetyltransferase (NAT) activity were investigated in low-density monolayer cultures of chick retinal cells, in which photoreceptors represented approximately 70% of the total cell population. NAT activity expressed by the...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Avendano G,Butler BJ,Michael Iuvone P

    更新日期:1990-01-01 00:00:00

  • Glutamine transport at the blood-brain and blood-cerebrospinal fluid barriers.

    abstract::Glutamine has multiple physiological and pathophysiological roles in the brain. Because of their position at the interface between blood and brain, the cerebral capillaries and the choroid plexuses that form the blood-brain barriers (BBB) and blood-cerebrospinal fluid (CSF) barriers, have the potential to influence br...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Xiang J,Ennis SR,Abdelkarim GE,Fujisawa M,Kawai N,Keep RF

    更新日期:2003-09-01 00:00:00

  • Axonal transports of tripeptidyl peptidase II in rat sciatic nerves.

    abstract::Axonal transport of tripeptidyl peptidase II, a putative cholecystokinin inactivating serine peptidase, was examined in the proximal, middle, and distal segments of rat sciatic nerves using a double ligation technique. Enzyme activity significantly increased not only in the proximal segment but also in the distal segm...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Chikuma T,Shimizu M,Tsuchiya Y,Kato T,Hojo H

    更新日期:2007-01-01 00:00:00

  • Induction of NADP-dependent malic dehydrogenase activity in brain of singi fish, heteropneustes fossilis (bloch), by 3,5,3?-triiodothyronine.

    abstract::For elucidation of thyroid hormone-induced responsiveness of fish brain, various doses (0.012, 0.025, 0.05, 0.1, 0.25, 0.5, 1, 2 and 4 ?g/g) of triiodothyronine (T(3)) were injected in Singi fish, Heteropneustes fossilis (Bloch), for 3 consecutive days and the changes in cytosolic NADP-dependent malic enzyme (ME, EC 1...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Srabani De AK,Ray DA,Medda AK

    更新日期:1989-01-01 00:00:00

  • AMPA receptor mediated excitotoxicity in neocortical neurons is developmentally regulated and dependent upon receptor desensitization.

    abstract::AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) excitotoxicity was examined in cultured neocortical neurons using the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to measure cell viability. Neurons were exposed to AMPA at different culture periods during development o...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Jensen JB,Schousboe A,Pickering DS

    更新日期:1998-05-01 00:00:00

  • Intracerebral VEGF injection highly upregulates AQP4 mRNA and protein in the perivascular space and glia limitans externa.

    abstract::Stroke is the third leading cause of death and the leading cause of adult disability in the industrialized nations. One of the consequences of stroke is blood-brain barrier (BBB) leakage and subsequent edema, which is one of the causes of mortality in this pathology. Aquaporin-4 (AQP4) is the most abundant water chann...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Rite I,Machado A,Cano J,Venero JL

    更新日期:2008-03-01 00:00:00

  • Ketogenic diet is antiepileptogenic in pentylenetetrazole kindled mice and decrease levels of N-acylethanolamines in hippocampus.

    abstract::The ketogenic diet (KD) is used for the treatment of refractory epilepsy in children, however, the mechanism(s) remains largely unknown. Also, the antiepileptogenic potential in animal models of epilepsy has been poorly addressed. Activation of cannabinoid type-1 receptor (CB(1)-R) upon seizure activity may mediate ne...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Hansen SL,Nielsen AH,Knudsen KE,Artmann A,Petersen G,Kristiansen U,Hansen SH,Hansen HS

    更新日期:2009-03-01 00:00:00

  • Differentiation of neuroblastoma cells by chick gizzard extract.

    abstract::Cholinergic neuroblastoma NS20Y cells were differentiated by the chicken gizzard extract. They were first inoculated into a glass culture bottle and the aggregated cells which grew in the suspension culture were collected. The aggregated cells (round and immature neuroblastoma cells) were seeded on a polyornithinecoat...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Miki N,Higashida H,Hayashi Y,Akiyama M,Kuo CH

    更新日期:1982-01-01 00:00:00

  • CaM kinase II in long-term potentiation.

    abstract::The observation that autophosphorylation converts CaM kinase II from the Ca(2+)-dependent form to the Ca(2+)-independent form has led to speculation that the formation of the Ca(2+)-independent form of the enzyme could encode frequency of synaptic usage and serve as a molecular explanation of "memory". In cultured rat...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审


    authors: Fukunaga K,Muller D,Miyamoto E

    更新日期:1996-04-01 00:00:00

  • Identification of an endogenous polypeptide modulating ligand binding sites of insect neuronal acetylcholine receptors.

    abstract::In this report evidence is presented for a membrane-associated polypeptide that regulates ligand binding properties of a neuronal acetylcholine receptor. Removal of membrane-associated compounds reversibly increased the number of BGTX binding sites and decreased the number of binding sites for ACh in neuronal membrane...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Benke D,Breer H

    更新日期:1990-01-01 00:00:00

  • Functional significance of the negative-feedback regulation of ATP release via pannexin-1 hemichannels under ischemic stress in astrocytes.

    abstract::The opening of pannexin-1 (Px1) hemichannels is regulated by the activity of P2X(7) receptors (P2X(7)Rs). At present, however, little is known about how extracellular ATP-sensitive P2X(7)Rs regulates the opening and closure of Px1 hemichannels. Several lines of evidence suggest that P2X(7)Rs are activated under pathol...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Iwabuchi S,Kawahara K

    更新日期:2011-02-01 00:00:00

  • Antioxidant potential of Minocycline in Japanese Encephalitis Virus infection in murine neuroblastoma cells: correlation with membrane fluidity and cell death.

    abstract::Minocycline is neuroprotective in animal models of a number of acute CNS injuries, neurodegenerative diseases and CNS infection. While anti-inflammatory and anti-apoptotic effects of Minocycline have been characterized, the molecular basis for the neuroprotective effects of Minocycline remains unclear. We report here ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Mishra MK,Ghosh D,Duseja R,Basu A

    更新日期:2009-06-01 00:00:00

  • Pyrrolidinedithiocarbamate induces apoptosis in cerebellar granule cells: involvement of AP-1 and MAP kinases.

    abstract::Pyrrolidinedithiocarbamate (PDTC) is a compound displaying antioxidant, pro-oxidant and metal chelator properties in different cell types. It has been described that PDTC may exert either anti-apoptotic or apoptotic activity. Moreover it is known that this agent regulates the activity of redox-sensitive transcription ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Porcile C,Stanzione S,Piccioli P,Bajetto A,Barbero S,Bisaglia M,Bonavia R,Florio T,Schettini G

    更新日期:2003-07-01 00:00:00

  • Scn1a and Cacna1a mutations mutually alter their original phenotypes in rats.

    abstract::This study aimed to examine the effects of Cacna1a mutation on the phenotype of Scn1a-associated epilepsy in rats. We used rats with an N1417H missense mutation in the Scn1a gene and others with an M251K mutation in the Cacna1a gene. Scn1a/Cacna1a double mutant rats were generated by mating both Scn1a and Cacna1a muta...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Ohmori I,Kobayashi K,Ouchida M

    更新日期:2020-12-01 00:00:00

  • Chlorimipramine, fenfluramine and quipazine decrease 5-hydroxytryptamine synthesis in discrete rat brain nuclei.

    abstract::A procedure for studying 5-hydroxytryptamine synthesis by determining the rate of accumulation of 5-hydroxytryptophan after administering m-hydroxybenzylhydrazine, an inhibitor of aromatic-l-amino acid decarboxylase, and large doses of l-tryptophan was characterized. The utility of this method as an index of 5-hydroxy...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Nielsen JA

    更新日期:1986-01-01 00:00:00

  • Glycine receptor subunits expression in the developing rat retina.

    abstract:BACKGROUND AND METHODS:Glycine receptor (GlyR) consists of two α (1-4) and three β subunits. Considerable evidence indicates that the adult retina expresses the four types of α subunits; however, the proportion of these subunits in adult and immature retina is almost unknown. In this report we have studied mRNA and the...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Sánchez-Chávez G,Velázquez-Flores MÁ,Ruiz Esparza-Garrido R,Salceda R

    更新日期:2017-09-01 00:00:00

  • Characterization of synaptosomes from the central nervous system of insects.

    abstract::Nerve terminals from the head ganglia of Locusta migratoria were isolated by means of a modified microscale flotation technique. Enzymatic, ultrastructural and chemical analysis revealed that the synaptosomal fraction was highly enriched in well-preserved nerve endings containing almost no free mitochondria. Cholinerg...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Breer H

    更新日期:1981-01-01 00:00:00

  • Inhibition of hippocampal LTP by ginkgolide B is mediated by its blocking action on PAF rather than glycine receptors.

    abstract::Platelet-activating factor (PAF), a biologically active lipid (1-O-alkyl-2-acetyl-sn-glycero-3-phosphoholine), is identified in different regions of brain, including hippocampus. Specific PAF-activated receptors (PAFRs) are expressed in corresponding brain areas. PAF has been proposed to be a retrograde messenger of l...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Kondratskaya EL,Pankratov YV,Lalo UV,Chatterjee SS,Krishtal OA

    更新日期:2004-02-01 00:00:00

  • Light exposure stimulates the activity of ganglioside glycosyltransferases of retina ganglion cells.

    abstract::In chicks submitted to light stimulation, the synthesis of gangliosides of the retina ganglion cell increases with respect to chicks maintained in the dark. In an attempt to elucidate if the activation of glycosyltransferases participates in the establishment of these light-dark differences detected in vivo, we examin...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Bussolino DF,Guido ME,Caputto BL

    更新日期:1997-07-01 00:00:00