Cloning and expression of human liver UDP-glucuronosyltransferase cDNA, UDPGTh2.


:The human liver cDNA clone UDPGTh2, encoding a liver UDP-glucuronosyltransferase (UDPGT) was isolated from a lambda gt 11 cDNA library by hybridization to mouse transferase cDNA clone, UDPGTm1. UDPGTh2 encoded a 529 amino acid protein with an amino terminus membrane-insertion signal peptide and a carboxyl terminus membrane-spanning region. There were three potential asparagine-linked glycosylation sites at residues 67, 68, and 315. In order to obtain UDPGTh2 protein encoded from cloned human liver UDP-glucuronosyltransferase cDNA, the clone was inserted into the pSVL vector (pUDPGTh2) and expressed in COS 1 cells. The presence of a transferase with Mr approximately 52,000 in transfected cells cultured in the presence of [(35)S]methionine was shown by immunocomplexed products with goat antimouse transferase IgG and protein A-Sepharose and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. The expressed UDPGT was a glycoprotein as indicated by electrophoretic mobility shift in Mr approximately 3,000-4,000 when expressed in the presence of tunicamycin. The extent of glycosylation was difficult to assess, although one could assume that glycosyl structures incorporated at the level of endoplasmic reticulum were always the core oligosaccharides. Thus, it is likely that at least two moieties inserted can account for the shift of Mr approximately 3,000-4,000. This study demonstrates the cDNA and deduced amino acid sequence of human liver UDP-glucuronosyltransferase cDNA, UDPGTh2.


Arch Pharm Res


Dong M,Owens IS,Sheen YY




Has Abstract


1997-10-01 00:00:00










  • Comparison of 2 methods of non-invasive treatment between transcutaneous electrical stimulation and pulsed electromagnetic field stimulation as replacement of invasive manual acupuncture.

    abstract::The aim of this study was to find the non-invasive optimal alternative method for Manual Acupuncture. Existing researches had reported that Transcutaneous Electrical Acupoint Stimulation (TEAS) was an effective treatment method instead of manual acupuncture. In place of the TEAS, we suggested the Pulsed Electromagneti...

    journal_title:Acupuncture & electro-therapeutics research

    pub_type: 临床试验,杂志文章


    authors: Kim SB,Kim JY,Park SW,Lee NR,Lee SW,Kim YH,Lee YH

    更新日期:2012-01-01 00:00:00

  • Effect of impulse vibration and noise on vasomotor function of peripheral blood vessels among pneumatic forge hammer operators.

    abstract::An evaluation of the effect of impulse vibration and noise on vasomotor function of blood vessels among pneumatic forge hammer operators has been presented based on thermal tests (cooling). The study covered the following groups of workers; pneumatic forge hammer operators (I), pneumatic forging hammer operators (II),...

    journal_title:Central European journal of public health

    pub_type: 杂志文章


    authors: Solecki L

    更新日期:1995-01-01 00:00:00

  • Occurrence of Levonorgestrel in Water Systems and Its Effects on Aquatic Organisms: A Review.

    abstract::Levonorgestrel is one of the active ingredients of oral contraceptives detected in the aquatic environment at concentrations in the order of ng/L. During the past decade, a wealth of new information about levonorgestrel has been produced, with several studies having reported negative effects in the reproduction and gr...

    journal_title:Reviews of environmental contamination and toxicology

    pub_type: 杂志文章


    authors: Oropesa AL,Guimarães L

    更新日期:2020-06-04 00:00:00

  • Focal segmental glomerulosclerosis with rapid decline in renal function ("malignant FSGS").

    abstract::We describe 11 patients whose renal biopsies showed minimal changes with focal segmental glomerulosclerosis. These patients, in contrast to the majority of patients with similar renal histology, went into renal failure within 2 1/2 years of clinical onset. All were young, severely nephrotic, most hypertensive, with mi...

    journal_title:Clinical nephrology

    pub_type: 杂志文章


    authors: Brown CB,Cameron JS,Turner DR,Chantler C,Ogg CS,Williams DG,Bewick M

    更新日期:1978-08-01 00:00:00

  • Evaluating Screening Tests for Depression in Post-Stroke Older Adults.

    abstract:BACKGROUND:Uncertainty surrounds which screening test to use in older patients with poststroke depression, in whom symptoms of depression are more complex and often occur in conjunction with other comorbidities. We evaluated screening tests for depression among a cohort of older ambulatory individuals with comorbid isc...

    journal_title:Journal of geriatric psychiatry and neurology

    pub_type: 杂志文章


    authors: Wang EY,Meyer C,Graham GD,Whooley MA

    更新日期:2018-05-01 00:00:00

  • [Immunitary response in tuberculosis and the role of Mycobacterium tuberculosis secretion antigens in its protection, pathology and diagnosis. Review].

    abstract::Tuberculosis (TB) is one of the main causes of death by M. tuberculosis infection in humans worldwide and, it is worrying that the number of cases have been increasing in recent years, largely due to HIV infection. Since the diagnostic methods for detecting the disease are not totally effective because of their lack o...

    journal_title:Investigacion clinica

    pub_type: 杂志文章,评审


    authors: Araujo Z,Acosta M,Escobar H,Baños R,Fernández de Larrea C,Rivas-Santiago B

    更新日期:2008-09-01 00:00:00

  • The role of immunohistochemistry in the diagnosis of urinary bladder neoplasms.

    abstract::The spectrum of neoplasms involving the urinary bladder is diverse, and, at times, different entities with distinct prognostic and managerial implications may have significant morphologic overlap. The presence of a neoplasm with an unusual morphologic appearance, such as an undifferentiated spindled, or plasmacytoid p...

    journal_title:Seminars in diagnostic pathology

    pub_type: 杂志文章,评审


    authors: McKenney JK,Amin MB

    更新日期:2005-02-01 00:00:00

  • Association of a Cor Triatriatum Sinister and a right partial anomalous pulmonary venous return. A case report.

    abstract::A 56-day old baby girl referred for recurrent respiratory tract infections and stridor, showed a rare association of a Cor Triatriatum Sinister (CTS) and a right Partial Anomalous Pulmonary Venous Return (PAPVR), in the form of a stenosed Scimitar vein, draining the right pulmonary veins to the subdiaphragmatic inferi...

    journal_title:Acta chirurgica Belgica

    pub_type: 杂志文章


    authors: Tueche SG,Demanet H,Goldstein JP,Dessy H,Viart P,Deuvaert FE

    更新日期:2005-04-01 00:00:00

  • Primary nursing in long-term care: is it economically feasible?

    abstract::1. The demand for nursing home beds exceeds supply, creating a sellers market. Administrators, therefore, do not see the need for making costly changes in the method of nursing care delivery. 2. ++Modified primary nursing, where permanent RN-nursing assistant teams are accountable to a permanent patient assignment, ca...

    journal_title:Journal of gerontological nursing

    pub_type: 杂志文章


    authors: Henry M

    更新日期:1990-10-01 00:00:00

  • Quantitative MRI analysis of idiopathic carpal tunnel syndrome.

    abstract:AIM:The aim of this study was to try to find parametric ratios for the diagnosis and pathophysiology of carpal tunnel syndrome using MR. MATERIAL AND METHODS:Dominant side wrist MRI examinations of 27 female carpal tunnel patients and 21 normal females were compared. The carpal tunnel contents area / carpal tunnel cro...

    journal_title:Turkish neurosurgery

    pub_type: 杂志文章


    authors: Oge HK,Acu B,Gucer T,Yanik T,Savlarli S,Firat MM

    更新日期:2012-01-01 00:00:00

  • Campylobacter jejuni induces diverse kinetics and profiles of cytokine genes in INT-407 cells.

    abstract:OBJECTIVE:To examine the kinetic ability of embryonic human epithelial INT-407 cells to express messenger ribonucleic acid mRNA for various cytokines and chemokines in response to Campylobacter jejuni C. jejuni stimulation. METHODS:In an experimental single-blind study, cultured embryonic human epithelial INT-407 cell...

    journal_title:Saudi medical journal

    pub_type: 杂志文章


    authors: Al-Amri AI,Botta GA,Tabbara KS,Ismaeel AY,Al-Mahmeed AE,Qareeballa AY,Bin Dayna KM,Bakhiet MO

    更新日期:2008-04-01 00:00:00

  • Contribution of CNT1 and ENT1 to ribavirin uptake in human hepatocytes.

    abstract::The objective of this study was to investigate the contributions of a sodium-dependent concentrative nucleoside transporter (CNT) 1 and an equilibrative nucleoside transporter (ENT) 1 to ribavirin uptake in human hepatocytes. The initial studies in oocytes expressing CNT1 and ENT1 showed increases in ribavirin uptake,...

    journal_title:Archives of pharmacal research

    pub_type: 杂志文章


    authors: Choi MK,Kim MH,Maeng HJ,Song IS

    更新日期:2015-01-01 00:00:00

  • Dendritic epidermal T-cell activation.

    abstract::Although gammadelta T cells compose a small proportion of lymphocytes in lymphoid compartments and peripheral blood, they are the major T-cell population present in epithelial tissues. However, the role played by gammadelta TCR expressing intraepithelial lymphocytes (IEL) has been enigmatic. The location of tissue-res...

    journal_title:Critical reviews in immunology

    pub_type: 杂志文章,评审


    authors: Sharp LL,Jameson JM,Witherden DA,Komori HK,Havran WL

    更新日期:2005-01-01 00:00:00

  • [Immune reconstitution inflammatory syndrome related with infliximab interruption in patient with Crohn's disease and active tuberculosis].

    abstract::Tumor necrosis factor alpha inhibitors are associated with an increased risk of active tuberculosis. However, its interruption in this setting may trigger a paradoxical response to tuberculosis treatment, as an immune reconstitution inflammatory syndrome. We present the case of a 36-year-old patient, with Crohn's dise...

    journal_title:Acta reumatologica portuguesa

    pub_type: 杂志文章


    authors: Carvalho J,Gonçalves C,Duque L,Brito P,Poças J

    更新日期:2014-10-01 00:00:00

  • [Clinical and pathological features in Venezuelan children with IgA nephropathy].

    abstract::IgA nephropathy (IgAN) is the most common glomerulonephritis in humans worldwide; its prevalence and prognosis vary according with geographical areas. The incidence is higher in adults under 30 years of age and in children, it occurs more frequently in patients between 3 and 10 years. Hematuria is the predominant mani...

    journal_title:Investigacion clinica

    pub_type: 杂志文章


    authors: Coronel V,Mora-Orta S,Domínguez L,Corado J,Zibaoui PI,Bethencourt S,Weffer M,Pinto JA,Lara E,Orta N

    更新日期:2007-06-01 00:00:00

  • Specific IgE in the identification of allergens in allergic rhinitis Malaysian patients.

    abstract::The specific serum IgE levels to 20 allergens were determined by enzyme immunoassay in 90 Malaysian patients with allergic rhinitis. Ninety-two percent of patients had elevated IgE to at least 1 of the allergens. The housedust mites D. pteronyssinus and D. farinae were the major allergens, elevated IgE to either aller...

    journal_title:Asian Pacific journal of allergy and immunology

    pub_type: 杂志文章


    authors: Choon-Kook S,Teck-Soong SL

    更新日期:1995-06-01 00:00:00

  • Detection of the FCGR3a polymorphism using a real-time polymerase chain reaction assay.

    abstract::The fragment crystallizable (Fc) region of the immunoglobulin G, low affinity III A receptor (FCGR3a, also known as CD16) belongs to the Fc gamma receptor family (FCGR), which plays an important role in immunoinflammatory processes. It is a low affinity, transmembrane receptor that is mainly expressed in monocytes, na...

    journal_title:Cancer genetics

    pub_type: 杂志文章


    authors: Burchard PR,Malhotra S,Kaur P,Tsongalis GJ

    更新日期:2013-04-01 00:00:00

  • Symptomatic pseudo-arthrosis of a trapezial ridge fracture.

    abstract::We present a fracture of the ridge of the trapezium with evolution towards a pseudo-arthrosis. ...

    journal_title:Acta chirurgica Belgica

    pub_type: 杂志文章


    authors: De Smet L,Degreef I

    更新日期:2007-01-01 00:00:00

  • A new calymmate mimosoid polyad from the Miocene of Argentina.

    abstract::A palynomorph with an unequivocal relationship to the eight-grain polyads of the mimosoid genus Calliandra Benth., is described from the Miocene sediments of San Juan Province, Argentina. Comparison of the fossil palynomorph with polyads of the extant Calliandra species shows a resemblance to those which have one, hig...

    journal_title:Review of palaeobotany and palynology

    pub_type: 杂志文章


    authors: Caccavari M,Barreda V V

    更新日期:2000-05-01 00:00:00

  • Liver-specific case fatality due to chronic hepatitis C virus infection: a systematic review.

    abstract::Despite reports that mortality is increasing, overall case fatality due to hepatitis C virus (HCV) is thought to be low. Given the variability in published rates, we aimed to synthesize estimates of liver-specific case fatality and all-cause mortality in chronic HCV according to follow-up duration, sustained viral res...

    journal_title:Annals of hepatology

    pub_type: 杂志文章,评审


    authors: Szabo SM,Samp JC,Walker DR,Lane S,Cline SK,Gooch KL,Jimenez-Mendez R,Levy AR

    更新日期:2015-09-01 00:00:00

  • [Cerebellar infarction].

    abstract::Cerebellar infarction can be difficult to diagnose because the clinical picture is often dominated by fairly non-specific symptoms, which are more indicative of a benign condition. When cerebellar infarction affects the brainstem, the semiology is richer, and pure cerebellar signs are rendered less important. A perfec...

    journal_title:Revue neurologique

    pub_type: 杂志文章,评审


    authors: Vuillier F,Decavel P,Medeiros de Bustos E,Tatu L,Moulin T

    更新日期:2011-05-01 00:00:00

  • [Necrotising endocarditis of mitral valve due to Staphylococcus lugdunensis].

    abstract::Staphylococcus lugdunensis is an infrequent cause of infective endocarditis (IE) and usually involves native valves of the heart. It causes life-threatening events such as rupture of cardiac valve or cerebral or pulmonary embolism due to necrosis on the endocardial tissue involved by the bacteria. Antibiotic therapy w...

    journal_title:Mikrobiyoloji bulteni

    pub_type: 杂志文章


    authors: Celebi G,Büyükateş M,Doğan SM,Pişkin N,Aydemir H,Oztoprak N,Aktaş E,Bektaş S,Keskin A,Akduman D

    更新日期:2009-04-01 00:00:00

  • 'Never again'. Model mugging: a therapeutic resource for the psychiatric nurse.

    abstract::I was in a room that had no windows and a disorienting effect. No light reached the room from outside and the fluorescent light gave the cement walls a forbidding appearance. The only sound was of my footsteps as they ricocheted off the walls, making them seem louder than ever. I felt cut off from the outside world. I...

    journal_title:Journal of psychosocial nursing and mental health services

    pub_type: 杂志文章


    authors: Ellensweig D

    更新日期:1997-06-01 00:00:00

  • Trichostatin A, lead compound for development of antifibrogenic drugs.

    abstract::Eukaryotic gene expression has mainly been studied in the context of trans-acting transcription factors and their interaction with regulatory cis-elements. Evidence is accumulating, that the higher order structure of chromatin also plays an essential role in eukaryotic gene expression. Hepatic stellate cells are the m...

    journal_title:Acta gastro-enterologica Belgica

    pub_type: 杂志文章


    authors: Rombouts K,Niki T,Wielant A,Hellemans K,Geerts A

    更新日期:2001-07-01 00:00:00

  • Facebook study: a little bit unethical but worth it?

    abstract::Human research involving the use social media raises many of the same issues as medical research. The publication of a paper in June 2014 investigating "emotional contagion" received extensive publicity recently because of the methods used. The approach involved manipulating the "News Feeds" of Facebook users, but the...

    journal_title:Journal of bioethical inquiry

    pub_type: 杂志文章


    authors: Kleinsman J,Buckley S

    更新日期:2015-06-01 00:00:00

  • Iatrogenic major vascular injury during lumbar discectomy: report of three cases.

    abstract::Iatrogenic vascular injury rarely occurs during lumbar disc surgery and can be fatal if it is not recognized instantly. In this paper we aim to introduce three iatrogenic vascular injuries that occurred during the lumbar disc surgery. The first case was consulted because of the sudden hypotension attack during lumbar ...

    journal_title:Turkish neurosurgery

    pub_type: 杂志文章


    authors: Keskin M,Serin KR,Genc FA,Aksoy M,Yanar F,Kurtoglu M

    更新日期:2013-01-01 00:00:00

  • Examining the properties and uses of two hydrogel sheet dressings.

    abstract::The purpose of this study was to review the physical characteristics and fluid-handling properties of two hydrogel sheet dressings in order to determine whether these materials possess any unique features which make them especially well suited to the treatment of specific types of problem wounds. ...

    journal_title:Journal of wound care

    pub_type: 杂志文章


    authors: Thomas S,Loveless P

    更新日期:1993-05-02 00:00:00

  • [Late hemorrhagic transformation of cerebellar infarction under anticoagulant treatment].

    abstract::Anticoagulant treatment after cerebral infarcts is still debated due to the imperfectly evaluated risk of hemorrhagic transformation. In the case reported here the hemorrhage developed during anticoagulant treatment instituted 18 days after the onset of the infarct. The role of previously administered aspirin is discu...

    journal_title:Revue neurologique

    pub_type: 杂志文章


    authors: Masson C,Charlier P,Masson M,Cambier J

    更新日期:1985-01-01 00:00:00

  • A first report on Hb Q-Iran in association with alpha-thalassemia in a case of spinal ischemia.

    abstract:BACKGROUND:Hemoglobin Q-Iran is a rare variant which has not been described in association with alpha-thalassemia to date. We present the case of a Turkish patient who developed spinal ischemia. METHODS:Spinal ischemia was diagnosed clinically, via magnetic resonance imaging and angiographically. Blood samples were an...

    journal_title:Clinical laboratory

    pub_type: 杂志文章


    authors: Zur B,Hildesheim A,Ludwig M,Stoffel-Wagner B

    更新日期:2011-01-01 00:00:00

  • Antibacterial drug prescription for outpatients: age, seasonal and pulmonary disease dependency.

    abstract::The goal of this study was to analyze antibacterial drug prescribing in the 1-million-plus, strictly defined population in Poland. For the 2002-2005 years, data from National Health Fund on outpatient purchasing of antibiotics in Lubuskie's Province were collected and expressed in DDD per 1-thousand inhabitants per da...

    journal_title:Acta poloniae pharmaceutica

    pub_type: 杂志文章


    authors: Dziurda DR,Polak S,Skowron A,Kuschill-Dziurda J,Brandys J

    更新日期:2008-05-01 00:00:00