Stimulation of troutCYP1A gene expression in mouse HEPA-1 cells by 3-methylcholanthrene.


:TroutCYP1A-CAT expression construct was generated by cloning approximately 3.5 Kb 5' flanking DNA of trout liverCYP1A gene in front of CAT gene at pCAT-basic vector. Hepa 1 cells, which are known to contain a functional arylhydrocarbon receptor' were transfected with troutCYP1A-CAT using lipofectin. 3-Methylcholanthrene (1 nM) was added into hepa 1 cells in culture in order to examine if 5' flanking DNA of troutCYP1A gene could interact with mouse transactivating factors to bring about transcription of the chloramphenicol acetyltransferase(CAT) reporter gene. The level of CAT protein was measured by CAT ELISA and the level of CAT mRNA was determined by RTPCR. The treatment of 1 nM 3-methylcholanthrene resulted in two fold increases in CAT protein as well as CAT mRNA compared to untreated control hepa 1 cells. These data indicate that arylhydrocarbon receptors of mouse hepa 1 cells are functional to activate exogenously transfected troutCYP1A-CAT construct in terms of both transcription and translation of CAT. We also examined the effect of 3-methylcholanthrene on endogeneouscyp1a1 activity in hepa 1 cell. 3-Methylcholanthrene (1 nM) treatment to hepa 1 cells transfected with troutCYP1A-CAT construct stimulated the level of cyp1a1 mRNA by two folds and the activity of ethoxyresorufin-O-deethylase by two fold compared to that of control cells. In this study we reported that troutCYP1A-CAT reporter gene expression construct could be expressed by 3-methylcholanthrene treatment in mouse hepa 1 cells. Thus troutCYP1A-CAT could serve as a good model to study the mechanism of regulation ofCYP1A1 gene expression.


Arch Pharm Res


Lee SY,Sheen YY




Has Abstract


1997-10-01 00:00:00










  • Hippocampal pathology in progressive supranuclear palsy (PSP): a quantitative study of 8 cases.

    abstract:OBJECTIVE:To quantify the neuronal and glial cell pathology in the hippocampus and the parahippocampal gyrus (PHG) of 8 cases of progressive supranuclear palsy (PSP). MATERIAL:tau-immunolabeled sections of the temporal lobe of 8 diagnosed cases of PSP. METHOD:The densities of lesions were measured in the PHG, CA sect...

    journal_title:Clinical neuropathology

    pub_type: 杂志文章


    authors: Armstrong RA,Lantos PL,Cairns NJ

    更新日期:2009-01-01 00:00:00

  • Country profile: leprosy in Brazil.

    abstract::Brazil has high rates of leprosy case detection, especially in the northern and west-central areas of the country. Effective decentralisation of routine treatment for leprosy has gathered pace since the year 2000 and this has improved access for patients, leading to a peak in new case detection in 2003 and a gradual d...

    journal_title:Leprosy review

    pub_type: 杂志文章


    authors: Penna ML,Grossi MA,Penna GO

    更新日期:2013-12-01 00:00:00

  • Evaluating the design of a family practice healthcare clinic using discrete-event simulation.

    abstract::With increased pressures from governmental and insurance agencies, today's physician devotes less time to patient care and more time to administration. To assist physician clinics in evaluating potential operating procedures that improve operating efficiencies and better satisfy patients, an object-oriented discrete-e...

    journal_title:Health care management science

    pub_type: 杂志文章


    authors: Swisher JR,Jacobson SH

    更新日期:2002-04-01 00:00:00

  • Caecum and ascending colon herniated in the lesser sac through the Winslow's hiatus. A case report.

    abstract::Herniation of the caecum and the ascending colon in the lesser sac through the Winslow's hiatus is an uncommon cause of intestinal occlusion. The clinical presentation can be acute or chronic. The diagnosis often remains unclear before surgical operation, even with the assistance of abdominal CT, providing an image of...

    journal_title:Acta chirurgica Belgica

    pub_type: 杂志文章


    authors: Jacquemin G,Boeur JP,Laloux P

    更新日期:2008-01-01 00:00:00

  • A prospective study for validation of Bayesian prediction approach to adjust individual lidocaine dosage.

    abstract::A Bayesian approach, in which the estimates of the population pharmacokinetic parameters of lidocaine reported by Vozeh et al. [1984 a and b] and one measurement of serum lidocaine concentration as feedback information were utilized, was prospectively evaluated in 20 Japanese patients. The concentration of lidocaine i...

    journal_title:International journal of clinical pharmacology and therapeutics

    pub_type: 杂志文章


    authors: Uematsu T,Hirayama H,Umemura K,Kosuge K,Nakashima M

    更新日期:1994-01-01 00:00:00

  • Megakaryocyte Polyploidization and Proplatelet Formation in Low-Attachment Conditions.

    abstract::In vitro-derived platelets (PLTs), which could provide an alternative source of PLTs for patient transfusions, are formed from polyploid megakaryocytes (MKs) that extend long cytoplasmic projections, termed proplatelets (proPLTs). In this study, we compared polyploidization and proPLT formation (PPF) of MKs cultured o...

    journal_title:Biochemical engineering journal

    pub_type: 杂志文章


    authors: Schlinker AC,Duncan MT,DeLuca TA,Whitehead DC,Miller WM

    更新日期:2016-07-15 00:00:00

  • [Ultrastructural and genetic aspects of epidermolysis bullosa albo-papuloidea (author's transl)].

    abstract::The authors report a case of epidermolysis bullosa dystrophica and albo-papuloidea, which led them to the discovery of eight similar cases in the same family. After analysing the main symptoms, including the histological ones, they underline the ultrastructural and genetic data. This disease can be described as a derm...

    journal_title:Annales de dermatologie et de venereologie

    pub_type: 杂志文章


    authors: Lambert D,Effantin C,Justrabo E,Gavanou J,Chapuis JL

    更新日期:1977-12-01 00:00:00

  • Frequency and clinical determinants of poststroke cognitive impairment in nondemented stroke patients.

    abstract::The objective of this study was to examine the prevalence and clinical correlates of poststroke cognitive impairment in Chinese stroke patients in Hong Kong. One hundred seventy-nine stroke patients were interviewed by a psychiatrist 3 months after their stroke. Cognitive impairment was determined according to the Min...

    journal_title:Journal of geriatric psychiatry and neurology

    pub_type: 杂志文章


    authors: Tang WK,Chan SS,Chiu HF,Ungvari GS,Wong KS,Kwok TC,Mok V,Wong KT,Richards PS,Ahuja AT

    更新日期:2006-06-01 00:00:00

  • Evaluation of six PCR assays in combination with patient related data for the diagnosis of Clostridium difficile-associated infections.

    abstract:BACKGROUND:The aim of this multicenter study was to establish a diagnostic algorithm using molecular methods for the diagnosis of C. difficile-associated infection (CDI). In addition patient specific data were taken into consideration for the interpretation of the results. METHODS:We compared the performance of six di...

    journal_title:Clinical laboratory

    pub_type: 杂志文章,多中心研究


    authors: Eigner U,Fenner I,Veldenzer A,Schwarz R,Oberdorfer K,Holfelder M

    更新日期:2014-01-01 00:00:00

  • Gene and environment interaction in familial suicidal behavior. A single family with 4 committed suicides.

    abstract::Family history of suicide is among the strongest predictors of suicide risk. From the context of gene by environment interactions, this manuscript presents a case study of the M family, which experienced 4 committed suicides within a short time period. Over the course of 5 years, the father and 3 sons committed suicid...

    journal_title:Saudi medical journal

    pub_type: 杂志文章


    authors: Khazaie H,Rezaie L,Alibakhshi R,Schwebel DC

    更新日期:2011-10-01 00:00:00

  • Web-based treatment of alcohol problems among rural women.

    abstract::It is estimated that 6 million women in the United States misuse alcohol. Of that number, many live in rural areas and face numerous barriers to treatment. The World Wide Web has the potential to help such individuals overcome these barriers. In light of emergent findings supporting the effectiveness of online alcohol...

    journal_title:Journal of psychosocial nursing and mental health services

    pub_type: 杂志文章,随机对照试验


    authors: Finfgeld-Connett D,Madsen R

    更新日期:2008-09-01 00:00:00

  • Glass inserts. A new dimension in restorative dentistry.

    abstract::Pediatric restorative dentistry has traditionally involved the placement of a stainless steel crown following a pulpotomy in primary molars. The increased exposure to systemic and topical fluorides has significantly decreased smooth surface caries, yet occlusal pit and fissure caries remains a problem. Occlusal caries...

    journal_title:American journal of dentistry

    pub_type: 杂志文章


    authors: Donly KJ,Ellis RK

    更新日期:1989-02-01 00:00:00

  • Tuberculosis and the elderly. A community health perspective.

    abstract::Tuberculosis is an ancient disease which has had a resurgence in the United States. Many elderly clients were exposed to tuberculosis as children and young adults and are still carrying the infection today. This article describes tuberculosis as it affects the elderly client in community and nursing home settings. The...

    journal_title:Journal of gerontological nursing

    pub_type: 杂志文章,评审


    authors: Gubser VL

    更新日期:1998-05-01 00:00:00

  • Predictability of serious adverse reaction alerts for monoclonal antibodies.

    abstract:OBJECTIVE:We analyzed the predictability of the United States Food and Drug Administration's Medwatch safety alerts on monoclonal antibodies with the aim of assessing the adequacy of their pre-approval safety evaluation. METHODS:An alert was considered observed when increased frequency, severity, or other new properti...

    journal_title:International journal of clinical pharmacology and therapeutics

    pub_type: 杂志文章


    authors: Stanulovic V,Zelko R,Kerpel-Fronius S

    更新日期:2011-03-01 00:00:00

  • Registered dietitians making a difference: early medical record documentation of estimated energy requirement in critically ill children is associated with higher daily energy intake and with use of the enteral route.

    abstract:BACKGROUND:Establishing a caloric requirement or energy target is a recommended part of any nutrition care plan. OBJECTIVE:Our objective was to describe early documentation of a caloric requirement in critically ill children, and to determine if this would have any effect on daily energy intake and route of nutrition....

    journal_title:Journal of the Academy of Nutrition and Dietetics

    pub_type: 杂志文章,多中心研究


    authors: Wakeham M,Christensen M,Manzi J,Kuhn EM,Scanlon M,Goday PS,Mikhailov TA

    更新日期:2013-10-01 00:00:00

  • [Vemurafenib-induced radiation recall dermatitis].

    abstract:INTRODUCTION:Radiation recall dermatitis is an uncommon inflammatory reaction of the skin appearing after several days to several years at the site of previous irradiation; it is precipitated by the use of triggering drugs, although rarely by BRAF or MEK inhibitors. PATIENTS AND METHODS:We report an unusual case of re...

    journal_title:Annales de dermatologie et de venereologie

    pub_type: 杂志文章


    authors: Greliak A,Le Guern A,Bataille M,Lebas D,Wiart T,Modiano P

    更新日期:2019-05-01 00:00:00

  • Vaspin as a Risk Factor of Insulin Resistance in Obstructive Sleep Apnea-Hypopnea Syndrome in an Animal Model.

    abstract:BACKGROUND:In this study, we aimed to establish a chronic intermittent hypoxia model in rats and explore the possible role of vaspin in insulin sensitivity. METHODS:Healthy male Wistar rats were randomly divided into two groups: normal control group (NC) and chronic intermittent hypoxia group (CIH). The NC group was r...

    journal_title:Clinical laboratory

    pub_type: 杂志文章


    authors: Zhuang X,Ni Y,Jiang D,Pu Y,Liu X,Chen S,Sun F,Sun A,Pan Z

    更新日期:2015-01-01 00:00:00

  • Supratentorial desmoplastic ependymoma with giant ependymal rosettes.

    abstract::We report a case of a 22-year-old female who presented with a solid tumor in the frontal lobe having no continuity with the wall of the lateral ventricle. The tumor was excised. The patient has been free from clinical symptoms and tumor recurrence for over nine years. Microscopically, the tumor was composed of extreme...

    journal_title:Clinical neuropathology

    pub_type: 杂志文章


    authors: Yoshida K,Sato K,Kubota T,Takeuchi H,Kitai R,Kashiwara K

    更新日期:2000-07-01 00:00:00

  • Spasmogenic and spasmolytic activities of Onosma griffithii Vatke.

    abstract::Methanolic extract of Onosma griffithii and its fractions were evaluated for possible effects on rabbits' jejunum preparations. Rabbits of either sex (weight 1.5-2.0 kg) were used in experiments. Studies were carried out on rabbits' jejunum preparations. Crude methanolic extract of Onosma griffithii (Meth.OG) was trie...

    journal_title:Pakistan journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Ali N,Ahmad B,Shah SW

    更新日期:2011-10-01 00:00:00

  • Prevalence and risk factors for Staphylococcus aureus and methicillin-resistant Staphylococcus aureus nasal carriage inpatients in a tertiary care hospital's chest clinic in Turkey.

    abstract:AIM:We aimed to determine the prevalence and associated risk factors for nasal methicillin-sensitive and methicillin-resistant Staphylococcus aureus (MSSA/MRSA) carriage among patients admitted to a chest clinic of a tertiary care hospital in this study. MATERIALS AND METHODS:Nasal samples were taken from anterior nar...

    journal_title:Nigerian journal of clinical practice

    pub_type: 杂志文章


    authors: Oguzkaya-Artan M,Artan C,Baykan Z

    更新日期:2016-05-01 00:00:00

  • [Crying provoked by sound stimuli in a case of probable dementia of Alzheimer's type].

    abstract::A case of pathological crying elicited only by non-verbal auditory stimulations in a woman with probable dementia of the Alzheimer type is reported. As neuropsychological data available in this case have suggested a greater involvement of left than right temporal cortex, the authors propose that the crying phenomenon ...

    journal_title:Revue neurologique

    pub_type: 杂志文章


    authors: Eustache F,Lechevalier B,Viader F,Chapon F,Morin I,Morin P

    更新日期:1988-01-01 00:00:00

  • Problematic and risky sexual behaviour under the influence of alcohol among university students.

    abstract:OBJECTIVE:The objective of this research was to determine the prevalence of problematic and risky sexual behaviour after alcohol consumption and the correlation between this prevalence and sex, behavioural factors, problematic drinking, and alcohol consumption characteristics. METHODS:A survey of students was carried ...

    journal_title:Central European journal of public health

    pub_type: 杂志文章


    authors: Petruželka B,Barták M,Rogalewicz V,Rosina J,Popov P,Gavurová B,Čierna M,Vaska L,Šavrnochová M,Dlouhý M

    更新日期:2018-12-01 00:00:00

  • Cerebello-brain stem orthochromatic leukodystrophy with floppiness and bulbar paralysis.

    abstract::A two year and ten-month-old girl with cerebello-brain stem leukodystrophy is presented. She was hypotonic with spasticity and showed delayed motor development until she was twelve months old, when regression of motor activities began. Progressive hypotonia, dysphagia and fatal respiratory abnormalities developed. Aud...

    journal_title:Clinical neuropathology

    pub_type: 杂志文章


    authors: Kaga M,Kawasaki M,Mizuno Y,Ohuchi M,Nagashima K,Mohri N

    更新日期:1984-07-01 00:00:00

  • [Effect of quinones and nitrofurans on Trypanosoma mega and Crithidia fasciculata].

    abstract::Demonstration of trypanocidal effects in vitro is a first step for the development of new antichagasic drugs. In order to obtain an experimental model allowing the pre-screening of potential trypanocides for Trypanosoma cruzi in a short time and under safe conditions, the trypanosomatids T. mega and C. fasciculata wer...

    journal_title:Revista Argentina de microbiologia

    pub_type: 杂志文章


    authors: de Pahn EM,Molina Portela MP,Stoppani AO

    更新日期:1988-07-01 00:00:00

  • Application of a first impression triage in the Japan railway west disaster.

    abstract::On April 25, 2005, a Japanese express train derailed into a building, resulting in 107 deaths and 549 injuries. We used "First Impression Triage (FIT)", our new triage strategy based on general inspection and palpation without counting pulse/respiratory rates, and determined the feasibility of FIT in the chaotic situa...

    journal_title:Acta medica Okayama

    pub_type: 杂志文章


    authors: Hashimoto A,Ueda T,Kuboyama K,Yamada T,Terashima M,Miyawaki A,Nakao A,Kotani J

    更新日期:2013-01-01 00:00:00

  • 12-Oxo-10-glutathionyl-5,8,14-eicosatrienoic acid (TOG10), a novel glutathione-containing eicosanoid generated via the 12-lipoxygenase pathway in human platelets.

    abstract::Biologically active glutathione (GSH) conjugates of oxygenated fatty acids comprise a group of pro- and anti-inflammatory lipid mediators. While arachidonic acid (AA)-derived conjugates, as the cysteinyl leukotrienes (cys-LTs) and eoxins (EXs) have pro-inflammatory properties, conjugates in tissue regeneration (CTRs) ...

    journal_title:Prostaglandins & other lipid mediators

    pub_type: 杂志文章


    authors: Liening S,Fischer J,Jagusch H,Pohnert G,Höcker O,Neusüß C,Werz O,Scriba GKE,Garscha U

    更新日期:2021-02-01 00:00:00

  • [Paroxysmal dystonia and multiple sclerosis].

    abstract:INTRODUCTION:Movement disorders are uncommon in multiple sclerosis, except for tremor. Patients rarely have paroxysmal dystonia (or tonic spasm), which can be the presenting manifestation of the disease. OBSERVATIONS:Two videotaped observations are presented. The first patient was a 27-year-old woman, treated for rela...

    journal_title:Revue neurologique

    pub_type: 杂志文章


    authors: El Otmani H,Benmansour Y,Araqi-Houssaini A,Benkirane N,Dany F,Abdoh Rafai M,El Moutawakil B,Slassi I

    更新日期:2014-02-01 00:00:00

  • Effect of hydrogen peroxide on VIP-induced relaxation of the cat lower esophageal sphincter.

    abstract::We investigated the effects of hydrogen peroxide (H2O2) on relaxation of the cat lower esophageal sphincter (LES). Vasoactive intestinal peptide (VIP) caused dose-dependent relaxation of LES, and H2O2 reduced VIP-induced relaxation. Relaxation was also attenuated by pertussis toxin (PTX), indicating a Gi/o component. ...

    journal_title:Archives of pharmacal research

    pub_type: 杂志文章


    authors: Kim SH,Youm JH,Lee DK,Park SY,Shin CY,Ryu JS,La HO,Song HJ,Min YS,Sohn UD

    更新日期:2007-11-01 00:00:00

  • Transnational corporations and oral health inequalities; an introduction.

    abstract::In an increasingly globalised world, Trans-National Corporations (TNCs) wield considerable social, economic and political influence, both in the international market economy and within individual countries. The practices of TNCs can have positive or adverse effects on population health through production methods and p...

    journal_title:Community dental health

    pub_type: 杂志文章


    authors: Jamieson LM

    更新日期:2019-05-30 00:00:00

  • Profile of women in a county jail.

    abstract::The literature about women in prison says little about characteristics of women in local jails. The goal of this study was to better understand the demographics and characteristics of these women to develop responsive programming. A cross-sectional survey of 346 women in an urban jail was conducted. Descriptive statis...

    journal_title:Journal of psychosocial nursing and mental health services

    pub_type: 杂志文章


    authors: Kelly PJ,Peralez-Dieckmann E,Cheng AL,Collins C

    更新日期:2010-04-01 00:00:00