Low density lipoprotein (LDL)-antioxidant flavonoids from roots of Sophora flavescens.


:Oxidation of low density lipoprotein (LDL) is strongly implicated as a key process in the onset of atherosclerosis. In this study, nine alkylated (C10-C5) flavonoids from Sophora flavescens were examined for their inhibitory effects on copper-induced LDL oxidation. Of the flavonoids tested, sophoraflavanone G (1), kurarinone (2), kurarinol (3), norkurarinol (4), and kuraridin (9) inhibited the generation of thiobarbituric acid reactive substances (TBARS) with IC50s of 7.9, 14.5, 22.0, 26.9, and 17.5 microM, respectively. The most potent inhibitor, compound 1, also demonstrated significant activities in complementary in vitro investigations, such as lag time (130 min at 5 microM), relative electrophoretic mobility (REM) of ox-LDL (80% inhibition at 20 microM), and fragmentation of apoB-100 (inhibition of 71% at 20 microM). Analysis of the structures of these compounds reveals that a resorcinol moiety in the B-ring is strongly correlated with protection of LDL-oxidation.


Biol Pharm Bull


Jeong TS,Ryu YB,Kim HY,Curtis-Long MJ,An S,Lee JH,Lee WS,Park KH




Has Abstract


2008-11-01 00:00:00














  • Effects of the acetylene compound from Atractylodes rhizome on experimental gastric ulcers induced by active oxygen species.

    abstract::This study was conducted to determine the beneficial effects of treating digestive disorders of (6E,12E)-tetradecadiene-8,10-diyne-1,3-diol diacetate (TDEYA) detected in the plasma in hydrolyzed form: (6E,12E)-tetradecadiene-8,10-diyne-1,3-diol (TDEY), following the oral administration of a decoction of Atractylodes r...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Sakurai T,Sugawara H,Saito K,Kano Y

    更新日期:1994-10-01 00:00:00

  • Pharmacological and pharmaceutical properties of freeze-dried formulations of egg albumin, indomethacin, olive oil, or fatty acids.

    abstract::Formulations consisting of egg albumin, indomethacin (IND), and olive oil or fatty acids, were prepared by vigorous stirring using a high-speed homogenizer and subsequent freeze-drying. To confirm the anti-inflammatory properties and ulcerogenic effects of the formulations, we examined the action of the formulations o...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Tsuji Y,Kakegawa H,Miyataka H,Matsumoto H,Satoh T

    更新日期:1993-07-01 00:00:00

  • Mutagenicities of 8-hydroxyguanine and 2-hydroxyadenine produced by reactive oxygen species.

    abstract::Oligodeoxyribonucleotides containing 8-hydroxyguanine and 2-hydroxyadenine, purine lesions produced in cells by reactive oxygen species, were synthesized and inserted into vector DNAs to introduce each lesion at a predetermined site. The manipulated DNAs were transfected into living cells, and the mutants induced by e...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章,评审


    authors: Kamiya H

    更新日期:2004-04-01 00:00:00

  • Formulation and evaluation of paclitaxel-loaded polymeric nanoparticles composed of polyethylene glycol and polylactic acid block copolymer.

    abstract::To develop potent paclitaxel (PTX) formulations for cancer chemotherapy, we formulated PTX into polymeric nanoparticles composed of polyethylene glycol (PEG) and polylactic acid (PLA) block copolymer (PN-PTX). First, the physicochemical properties of PN-PTX prepared were assessed; the mean particle size was around 80 ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Araki T,Kono Y,Ogawara K,Watanabe T,Ono T,Kimura T,Higaki K

    更新日期:2012-01-01 00:00:00

  • A novel immunomodulator, FTY720, prevents development of experimental autoimmune myasthenia gravis in C57BL/6 mice.

    abstract::Prophylactic oral administration of a novel immunomodulator (immunosuppressant), FTY720 (1 mg/kg, three times a week), completely prevented the development of experimental autoimmune myasthenia gravis (EAMG) in C57BL/6 mice. EAMG has been used as an animal model for human myasthenia gravis, and was established by immu...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kohno T,Tsuji T,Hirayama K,Iwatsuki R,Hirose M,Watabe K,Yoshikawa H,Kohno T,Matsumoto A,Fujita T,Hayashi M

    更新日期:2005-04-01 00:00:00

  • Alterations of metal content in the kidneys of puromycin aminonucleoside-administered rats.

    abstract::To explore the mechanism responsible for puromycin aminonucleoside induced-nephrotoxicity, metal content was preliminarily investigated in the kidneys of rats that had received a single subcutaneous injection of the agent (80 mg/kg body weight). In the group that received this agent, the renal content of Fe rose on da...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Fukata M,Hayashi T,Terui J,Sudo J

    更新日期:1994-06-01 00:00:00

  • Effects of liposome size on penetration of dl-tocopherol acetate into skin.

    abstract::Liposomes were prepared from hydrogenated lecithin (H-PC) by sonication (S) or injection (I) of H-PC dissolved in ethanol containing dl-tocopherol acetate (VEA). The effects of liposomes on the dermal absorption of VEA were studied. The particle diameter of S-liposomes was smaller than that of I-liposomes. The penetra...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Natsuki R,Morita Y,Osawa S,Takeda Y

    更新日期:1996-05-01 00:00:00

  • Quercetin attenuates oxygen-glucose deprivation- and excitotoxin-induced neurotoxicity in primary cortical cell cultures.

    abstract::The possible role of quercetin, a naturally occurring plant flavonoid, in protecting against oxygen-glucose deprivation (OGD)-, excitotoxins-, and free radical-induced neuronal injury in mouse cortical cell cultures was investigated. Pre- and co-treatment with quercetin (100 microM) inhibited 50 min OGD-, 20 microM N-...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ha HJ,Kwon YS,Park SM,Shin T,Park JH,Kim HC,Kwon MS,Wie MB

    更新日期:2003-04-01 00:00:00

  • Effect of aging on the intestinal transport of hydrophilic drugs in the rat small intestine.

    abstract::The effect of aging on the intestinal transport of hydrophilic drugs (and probe compounds) was investigated in the rat small intestine. Passive transport was suggested to be unchanged with aging from 8 (young) to 54 (old) and further to 101 (very old) weeks old, as shown for D-xylose and urea in single-pass intestinal...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yuasa H,Soga N,Kimura Y,Watanabe J

    更新日期:1997-11-01 00:00:00

  • Pharmacological properties of traditional medicines (XXVII). Interaction between Ephedra Herb and Gypsum under hyperthermal conditions in rats.

    abstract::There are many important considerations in the interactions among the herbal constituents in a prescription of traditional Chinese medicine (TCM). Ephedra Herb [Chinese characters: see text] (Eph) is described a warm and acrid agent in TCM. The combination of Eph and Gypsum [Chinese characters: see text] (Eph-Gyp) sho...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yuan D,Sunouchi H,Sakurai T,Saito K,Kano Y

    更新日期:2002-07-01 00:00:00

  • Development of a thermoelectric cooling apparatus for high-voltage isoelectric focusing on a cellulose acetate membrane.

    abstract::To develop an isoelectric focusing apparatus using a cellulose acetate membrane (Separax EF), we have designed a thermoelectric cooling isoelectric apparatus. This apparatus has two characteristics. Firstly, the cooling system was switched to a thermoelectric cooling system from an ice-cooling system. Secondly, the ch...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Shiba K,Toda T,Iijima S,Inoue J,Yoshida T,Cho H,Kimura M

    更新日期:1994-10-01 00:00:00

  • Expression of the ligand-binding domain-containing region of retinoic acid receptors alpha, beta and gamma in Escherichia coli and evaluation of ligand-binding selectivity.

    abstract::The complete molecule or the ligand-binding domain-containing region of each of the three subtypes of human retinoic acid receptors (hRAR alpha, hRAR beta and hRAR gamma) was expressed in Escherichia coli. The expressed recombinant RARs (rRARs: rRAR alpha/E, rRAR beta/E and rRAR gamma) showed nearly the same magnitude...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Fukasawa H,Iijima T,Kagechika H,Hashimoto Y,Shudo K

    更新日期:1993-04-01 00:00:00

  • Evaluation of Duloxetine as Micronuclei Inducer in an Acute and a Subchronic Assay in Mouse.

    abstract::Duloxetine is a widely used antidepressant worldwide. In the present report, we evaluated its capacity to induce micronucleated polychromatic erythrocytes (MNPEs) and micronucleated normochromatic erythrocytes (MNNEs) in mice. Two assays were performed, one with a single chemical administration and the other with dail...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Madrigal-Bujaidar E,Álvarez-González I,Madrigal-Santillán EO,Morales-González JA

    更新日期:2015-01-01 00:00:00

  • Increased in vitro release of interferon-gamma from ampicillin-stimulated peripheral blood mononuclear cells in Stevens-Johnson syndrome.

    abstract::Stevens-Johnson syndrome (SJS) was diagnosed in a 39-year-old woman, treated with ampicillin (4000-8000 mg daily), phenytoin (250 mg daily), and furosemide (20-40 mg daily) for 25, 21, and 20 d, respectively, before the appearance of the eruption. The lymphocyte stimulation test with the MTT [3-(4,5-dimethyl thiazol-2...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yoshimura T,Hamaguchi E,Usami E,Nakashima K,Kawaguchi M,Suzuki N,Okamoto Y,Nakao T,Yamazaki F

    更新日期:2004-06-01 00:00:00

  • Neuroprotection of nicotiflorin in permanent focal cerebral ischemia and in neuronal cultures.

    abstract::Nicotiflorin is a single component extracted from traditional Chinese medicine Flos Carthami. In this study, we investigated its neuroprotection in permanent focal cerebral ischemia model in rats, and in an in vitro model of ischemia. At doses of 2.5, 5 and 10 mg/kg, nicotiflorin administered immediately after the ons...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Li R,Guo M,Zhang G,Xu X,Li Q

    更新日期:2006-09-01 00:00:00

  • Prostacyclin (PGI) receptor binding and cyclic AMP synthesis activities of PGI1 analogues, SM-10906 and its methyl ester, SM-10902, in mastocytoma P-815 cells.

    abstract::The prostacyclin I1 (PGI1) analogue, SM-10906 and its methyl ester, SM-10902, have been compared with the PGI2 analogue, iloprost, with respect to binding to the PGI2 receptor, stimulation of adenylate cyclase activity and inhibition of thrombin-induced Ca2+ mobilization in mastocytoma P-815 cells. SM-10906 displaced ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Oka M,Negishi M,Yamamoto T,Satoh K,Hirohashi T,Ichikawa A

    更新日期:1994-01-01 00:00:00

  • The aversive, anxiolytic-like, and verapamil-sensitive psychostimulant effects of pulegone.

    abstract::We investigated the psychostimulant, rewarding, and anxiolytic-like effects of pulegone. Possible interactions between pulegone and menthol concerning their psychostimulant effect were also analyzed. General mouse activity after pulegone treatment, and the interacitons between pulegone and menthol, were determined in ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: da Silveira NS,de Oliveira-Silva GL,Lamanes Bde F,Prado LC,Bispo-da-Silva LB

    更新日期:2014-01-01 00:00:00

  • Improving the stability of short hairpin RNA against fetal bovine serum using the third double-stranded RNA-binding domain from Staufen protein.

    abstract::RNA interference (RNAi) is a technology that specifically inhibits gene expression and is carried out by small 21-27-nucleotide double-stranded small interfering RNA (siRNA) or short hairpin RNA (shRNA). RNAi has become a very promising technology for genetic research, however problems remain with the delivery of siRN...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kawaguchi Y,Danjo K,Okuda T,Okamoto H

    更新日期:2009-02-01 00:00:00

  • Allergen Stability and Immunological Reactivity during Co-dissolution and Incubation of House Dust Mite and Japanese Cedar Pollen SLIT-Tablets.

    abstract::Japanese allergic subjects are commonly sensitized to both house dust mite (HDM) and Japanese cedar pollen (JCP) and combined treatment with sublingual immunotherapy (SLIT) tablets is desirable. However, mixing extracts of two non-homologous allergens may compromise allergen stability and affect the clinical outcome. ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Watanabe A,Yamamoto T,Matsuhara H,Matsui H,Nakazawa H,Lund K,Ohashi-Doi K

    更新日期:2020-10-01 00:00:00

  • Determination of urinary and serum pentosidine and its application to elder patients.

    abstract::Pentosidine, a fluorescent cross-linking compound, accumulates in extracellular matrix, especially in the collagen, and is formed by the nonenzymatic process of advanced Maillard reaction. We developed a method of determination of pentosidine and tried to examine its level in urine and serum of elder patients. The met...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yoshihara K,Nakamura K,Kanai M,Nagayama Y,Takahashi S,Saito N,Nagata M

    更新日期:1998-10-01 00:00:00

  • Fibroin-derived peptides stimulate glucose transport in normal and insulin-resistant 3T3-L1 adipocytes.

    abstract::Fibroin, the protein of silk, and hydrolyzed fibroin have recently been described to enhance insulin sensitivity and glucose metabolism in 3T3-L1 adipocytes. Here, we report that a series of synthetic peptides derived from the fibroin sequence have enhancing effects on glucose transport in normal and insulin-resistant...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kim ED,Bayaraa T,Shin EJ,Hyun CK

    更新日期:2009-03-01 00:00:00

  • Expression and Characterization of the Human Intestinal Bacterial Enzyme Which Cleaves the C-Glycosidic Bond in 3″-Oxo-puerarin.

    abstract::Puerarin (daidzein 8-C-glucoside) is an isoflavone C-glucoside contained in the roots of Pueraria lobata OHWI. We have previously isolated the human intestinal bacterium, strain PUE, which metabolizes puerarin to daidzein, though the enzyme which cleaves C-glycosidic bond has not been clarified. Here, we identified on...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Nakamura K,Zhu S,Komatsu K,Hattori M,Iwashima M

    更新日期:2019-03-01 00:00:00

  • Effects of 3-O-methyldopa, L-3,4-dihydroxyphenylalanine metabolite, on locomotor activity and dopamine turnover in rats.

    abstract::It has been well known that 3-O-methyldopa (3-OMD) is a metabolite of L-3,4-dihydroxyphenylalanine (L-DOPA) formed by catechol O-methyltransferase (COMT), and 3-OMD blood level often reaches higher than physiological level in Parkinson's disease (PD) patients receiving long term L-DOPA therapy. However, the physiologi...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Onzawa Y,Kimura Y,Uzuhashi K,Shirasuna M,Hirosawa T,Taogoshi T,Kihira K

    更新日期:2012-01-01 00:00:00

  • Fluorescence investigation of the retinal delivery of hydrophilic compounds via liposomal eyedrops.

    abstract::We examined the feasibility of using submicron-sized liposomes (ssLips) for retinal delivery of hydrophilic compounds, which would also have a wide range of applications. To evaluate the uptake into conjunctival cell line and the intraocular behavior of hydrophilic compound-containing ssLips after eyedrop application,...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Hironaka K,Fujisawa T,Sasaki H,Tozuka Y,Tsuruma K,Shimazawa M,Hara H,Takeuchi H

    更新日期:2011-01-01 00:00:00

  • The liquid culture filtrates of entomogenous fungus Paecilomyces tenuipes and its glycoprotein constituent protects against anemia in mice treated with 5-fluorouracil.

    abstract::The purpose of the present study was to investigate the efficacy of a liquid culture filtrates of the entomogenous fungus Paecilomyces tenuipes (PTCF) and its main active glycoprotein-enriched (PGF) fraction against hematotoxicity in mice treated with 5-fluorouracil (5-FU). Oral administration of PTCF (100 mg/kg/d) fo...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Takata T,Tanaka T,Yahagi N,Yahagi R,Tsuchida H,Ishigaki Y,Tomosugi N,Fushiya S,Takano F,Ohta T

    更新日期:2008-08-01 00:00:00

  • Transport mechanism of anthracycline derivatives in human leukemia cell lines: uptake and efflux of daunorubicin and doxorubicin in HL60 and its resistant cells and comparison with those of pirarubicin.

    abstract::We examined the transport mechanisms of daunorubicin (DNR) and doxorubicin (ADR) in HL60 and HL60/THP cells which were the non-P-glycoprotein-mediated resistant clone of the parent HL60 cells and showed a low degree of resistance, and compared them with those of pirarubicin (THP). In both lines, it appeared that the u...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Nagasawa K,Natazuka T,Nomiyama M,Ohnishi N,Yokoyama T

    更新日期:1996-01-01 00:00:00

  • Comparative study of in vitro digestibility of food proteins and effect of preheating on the digestion.

    abstract::Information on the comparative digestibility of food allergens and non-allergenic proteins is crucial when stability to digestion is to be used as a criterion to assess the allergenic potential of novel proteins. Preheating effect on in vitro digestibility has not been fully examined. In this study we investigated the...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Takagi K,Teshima R,Okunuki H,Sawada J

    更新日期:2003-07-01 00:00:00

  • Intradermal concentration of hydroquinone after application of hydroquinone ointments is higher than its cytotoxic concentration.

    abstract::Ointments of the skin depigmentation agent hydroquinone (HQ) have been prepared by extemporaneous nonsterile compounding in our hospital. The HQ ointments were highly effective in the treatment of various types of skin pigmentations; however, various problems have emerged including chromatic aberration of the ointment...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Matsubayashi T,Sakaeda T,Kita T,Kurimoto Y,Nakamura T,Nishiguchi K,Fujita T,Kamiyama F,Yamamoto A,Okumura K

    更新日期:2003-09-01 00:00:00

  • Shikonin induces apoptosis through reactive oxygen species/extracellular signal-regulated kinase pathway in osteosarcoma cells.

    abstract::Shikonin, a major ingredient in the Chinese traditional herb Lithospermum erythrorhixon, exhibits multiple biological functions including antimicrobial, anti-inflammatory, and antitumor effects. In this study, we delineated the molecular mechanisms of shikonin in the apoptosis of 143B osteosarcoma cells. Shikonin redu...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Chang IC,Huang YJ,Chiang TI,Yeh CW,Hsu LS

    更新日期:2010-01-01 00:00:00

  • Sex difference in ischemic acute renal failure in rats: approach by proteomic analysis.

    abstract::It is known that female rats are resistant to ischemic acute renal failure (ARF), compared with male rats. To elucidate sex differences in ischemic ARF, we searched global protein expression in post-ischemic kidneys using proteomic techniques. Ischemic ARF was induced by 45-min ischemia followed by reperfusion. By pro...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Takayama J,Takaoka M,Sugino Y,Yamamoto Y,Ohkita M,Matsumura Y

    更新日期:2007-10-01 00:00:00