Purification and characterization of S-adenosyl-L-methionine: caffeic acid 3-O-methyltransferase from suspension cultures of alfalfa (Medicago sativa L.).


:Caffeic acid O-methyltransferase (COMT) is one of a group of proteins present in alfalfa cell cultures which can be photoaffinity labeled with S-adenosyl-L-[methyl-3H]methionine. The enzyme was purified to homogeneity from elicitor-treated suspension cultures and shown to exist as an active monomer of subunit Mr 41,000. COMT could be separated into two forms on the basis of their isoelectric points and relative affinities for S-adenosyl-methionine and S-adenosylhomocysteine. Both forms had equal affinities for caffeic acid, were highly specific for the 3-hydroxyl group of substituted cinnamic acids, and exhibited negligible activity toward flavonoid substrates. An antiserum raised against COMT from aspen immunoprecipitated alfalfa COMT activity. Peptide mapping studies indicated that the two forms of COMT and an isoflavone O-methyltransferase from alfalfa are closely related proteins. The extractable activity of COMT doubled over a 48-h period following exposure of alfalfa cell suspensions to a yeast elicitor preparation, and this was associated with a small change in the relative proportions of the two forms of the enzyme.


Arch Biochem Biophys


Edwards R,Dixon RA




Has Abstract


1991-06-01 00:00:00














  • Expression of UDP-glucuronosyltransferases (UGTs) 2B7 and 1A6 in the human brain and identification of 5-hydroxytryptamine as a substrate.

    abstract::The extrahepatic expression of UDP-glucuronosyltransferases (UGTs) is important in the detoxification of a number of endogenous and exogenous compounds, including 5-hydroxytryptamine and morphine. Studies were designed to investigate the extrahepatic expression of human UGTs using RT-PCR techniques and to determine th...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: King CD,Rios GR,Assouline JA,Tephly TR

    更新日期:1999-05-01 00:00:00

  • (6R)-5,6,7,8-tetrahydro-L-biopterin modulates nitric oxide-associated soluble guanylate cyclase activity in the rat cerebellum.

    abstract::(6R)-5,6,7,8-Tetrahydro-L-biopterin (R-THBP) is a cofactor not only for aromatic amino acid hydroxylases in mammalian tissues but also for nitric oxide synthase (NOS) induced by endotoxins or cytokines in some kinds of cells. Recently it has been reported that nitric oxide (NO) has biological activity in endothelium a...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Nunokawa Y,Ishihara T,Kanai T,Noguchi T

    更新日期:1992-11-01 00:00:00

  • Covalent immobilization of a halophilic, alkalithermostable lipase LipR2 on Florisil® nanoparticles for production of alkyl levulinates.

    abstract::A novel halophilic, alkalithermostable lipase LipR2 from Alkalispirillum sp. NM-ROO2 was cloned and expressed. LipR2 was covalently immobilized on Florisil® functionalized with glutaraldehyde. Protein binding efficiency of functionalized Florisil® was 94.7%. Immobilized LipR2 retained 97.5% of specific activity of the...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Mesbah NM

    更新日期:2019-05-30 00:00:00

  • The how's and why's of protein folding intermediates.

    abstract::The nature and role of intermediates have been the subject of much heated debate in the field of protein folding. Historically, intermediates were viewed as essential stepping-stones that guide a protein through the folding process to the native state. However, with the experimental identification of numerous small pr...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Tsytlonok M,Itzhaki LS

    更新日期:2013-03-01 00:00:00

  • Divergent folding pathways of two homologous proteins, BPTI and tick anticoagulant peptide: compartmentalization of folding intermediates and identification of kinetic traps.

    abstract::Bovine pancreatic trypsin inhibitor (BPTI) and tick anticoagulant peptide (TAP) are two structurally homologous proteins, which have been shown to exhibit distinct mechanisms of oxidative folding. We demonstrate here differences of their folding properties using the technique of disulfide scrambling. Two extensively u...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Chang JY,Li L

    更新日期:2005-05-01 00:00:00

  • The effect of putative nucleation sites on the loading and stability of iron in ferritin.

    abstract::The L chain of the iron storage protein ferritin contains more putative nucleation sites in the core (Glu53, 56, 57, 60, and 63) than does the H chain (Glu61, 64, and 67). Recombinant DNA techniques were used to investigate the role of these putative nucleation sites on iron loading by ceruloplasmin and on the stabili...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Juan SH,Aust SD

    更新日期:1998-02-15 00:00:00

  • Relationships between the rate of synthesis of ATP and the concentrations of reactants and products of ATP hydrolysis in maize root tips, determined by 31P nuclear magnetic resonance.

    abstract::Using 31P NMR spectroscopy, we have measured the rate of ATP synthesis, and the free concentrations of ATP, ADP, cytoplasmic Pi, and H+ in maize root tips under a wide range of conditions. We show that the ratio [ATP]/[ADP] in normoxic root tips is greater than 25. We found no simple relationship between the concentra...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Roberts JK,Lane AN,Clark RA,Nieman RH

    更新日期:1985-08-01 00:00:00

  • Small-angle x-ray scattering of high- and low-affinity heparin.

    abstract::Solution characterization of heparin with high affinity (HA) and low affinity (LA) for antithrombin III was performed using the methods of small-angle x-ray scattering (SAXS), viscometry, and aqueous gel permeation chromatography (GPC). SAXS provided various topological parameters including the radius of gyration ([S2...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Khorramian BA,Stivala SS

    更新日期:1986-06-01 00:00:00

  • Dulaglutide inhibits high glucose- induced endothelial dysfunction and NLRP3 inflammasome activation.

    abstract::Activation of the NLRP3 inflammasome plays an important role in high glucose- induced endothelial dysfunction in patients with type 2 diabetes mellitus (T2DM). Dulaglutide, a newly developed glucagon-like peptide-1 receptor (GLP-1R) agonist, has been approved for the management of T2DM. In the current study, we aimed ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Luo X,Hu Y,He S,Ye Q,Lv Z,Liu J,Chen X

    更新日期:2019-08-15 00:00:00

  • The binding of 8-anilino-1-naphthalene sulfonate (ANS) to fish myosin and the effect of salts on the thermal transitions of fish myosin-ANS complex.

    abstract::Myosin prepared from tilapia (Serotherodon aureus) was complexed with 8-anilino-1-naphthalene sulfonate (ANS) and continuously heated at 1 degree C/min. A large increase in fluorescence was observed with a transition temperature of 34 degrees C. The effect of several salts on the transition temperature was tested. A p...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wicker L,Knopp JA

    更新日期:1988-11-01 00:00:00

  • Stimulation of the DNA unwinding activity of human DNA helicase II/Ku by phosphorylation.

    abstract::The Ku autoantigen is a heterodimeric protein of 70- and 83-kDa subunits, endowed with duplex DNA end-binding capacity and DNA helicase activity (Human DNA Helicase II, HDH II). HDH II/Ku is well established as the DNA binding component, the regulatory subunit as well as a substrate for the DNA-dependent protein kinas...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ochem AE,Rechreche H,Skopac D,Falaschi A

    更新日期:2008-02-01 00:00:00

  • Sphingosine-1-phosphate activates phospholipase D in human airway epithelial cells via a G protein-coupled receptor.

    abstract::Sphingosine-1-phosphate (SPP) acts as a first messenger in immortalized human airway epithelial cells (CFNPE9o(-)), possibly interacting with an Edg family receptor. Expression of the SPP receptors Edg-1 and Edg-3, as well as a low level of Edg-5/H218, was detected in these cells, in agreement with their ability to sp...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Orlati S,Porcelli AM,Hrelia S,Van Brocklyn JR,Spiegel S,Rugolo M

    更新日期:2000-03-01 00:00:00

  • Selective inactivation of redox-sensitive mitochondrial enzymes during cardiac reperfusion.

    abstract::Reperfusion of ischemic myocardial tissue results in an increase in mitochondrial free radical production and declines in respiratory activity. The effects of ischemia and reperfusion on the activities of Krebs cycle enzymes, as well as enzymes involved in electron transport, were evaluated to provide insight into whe...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sadek HA,Humphries KM,Szweda PA,Szweda LI

    更新日期:2002-10-15 00:00:00

  • Efflux mechanism and pathway of verapamil pumping by human P-glycoprotein.

    abstract::Multidrug resistance (MDR) caused by overexpressed permeability-glycoprotein (P-gp) in cancer cells is the main barrier for the cure of cancers. P-gp can pump many chemotherapeutic drugs, which is a viable target to overcome P-gp-mediated MDR by efficient inhibitors of P-gp. However, limited understanding of the efflu...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wang L,Sun Y

    更新日期:2020-12-15 00:00:00

  • Carbon-13 isotope effects on reactions involving carbamate and carbamoyl phosphate.

    abstract::Several 13C isotope effects of relevance to reactions involving carbamate and carbamoyl phosphate have been determined. The fractionation factor of carbamate relative to aqueous CO2 is 1.011; the equilibrium isotope effect on the reaction catalyzed by carbamate kinase is 0.9983. From these data we can calculate that t...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Tipton PA,Cleland WW

    更新日期:1988-01-01 00:00:00

  • Comparative kinetic studies of the dealkylation reaction and steroid hydroxylations in various oxygen and carbon monoxide:oxygen atmospheres.

    abstract::The time-course kinetics of the cytochrome P-450-catalyzed dealkylations of the exogenous compounds benzphetamine, ethylmorphine, codeine, and 7-ethoxycoumarin were compared to the hydroxylation of the endogenous compound testosterone. Using liver microsomes from phenobarbital-induced rats, the time course of the deme...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Hamill SJ,Cooper DY,Schleyer H,Rosenthal O

    更新日期:1983-07-15 00:00:00

  • Directed mutagenesis of the dicyclohexylcarbodiimide-reactive carboxyl residues in beta-subunit of F1-ATPase of Escherichia coli.

    abstract::Previous studies in which dicyclohexylcarbodiimide (DCCD) was used to inactivate F1-ATPase enzymes have suggested that two glutamate residues in the beta-subunit are essential for catalysis. In the Escherichia coli F1-ATPase, these are residues beta-Glu-181 and beta-Glu-192. Oligonucleotide-directed mutagenesis was us...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Parsonage D,Wilke-Mounts S,Senior AE

    更新日期:1988-02-15 00:00:00

  • Testosterone alleviates mitochondrial ROS accumulation and mitochondria-mediated apoptosis in the gastric mucosa of orchiectomized rats.

    abstract::The effects of testosterone propionate (TP) on the oxidative stress and mitochondrial function, as well as on mitochondria associated apoptotic signaling, were analyzed in the gastric mucosa in orchiectomized male rats. The present study showed that testosterone deficiency triggered apoptosis by damaging the mitochond...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kang J,Jia Z,Ping Y,Liu Z,Yan X,Xing G,Yan W

    更新日期:2018-07-01 00:00:00

  • Significance of H461 at subsite +1 in substrate binding and transglucosylation activity of amylomaltase from Corynebacterium glutamicum.

    abstract::Amylomaltase (AM) catalyzes inter- and intra-molecular transglycosylation reactions of glucan to yield linear and cyclic oligosaccharide products. The functional roles of the conserved histidine at position 461 in the active site of AM from Corynebacterium glutamicum (CgAM) was investigated. H461 A/S/D/R/W were constr...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Tumhom S,Krusong K,Kidokoro SI,Katoh E,Pongsawasdi P

    更新日期:2018-08-15 00:00:00

  • Regulation of glycogen concentration and glycogen synthase activity in skeletal muscle of insulin-resistant rats.

    abstract::The aim of this study was to investigate the effect of insulin resistance on glycogen concentration and glycogen synthase activity in the red and white gastrocnemius muscles and to determine whether the inverse relationship existing between glycogen concentration and enzyme activity is maintained in insulin resistant ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Coderre L,Vallega GA,Pilch PF,Chipkin SR

    更新日期:2007-08-01 00:00:00

  • Elevated cholesterol and decreased sterol carrier protein-2 in peroxisomes from AS-30D hepatoma compared to normal rat liver.

    abstract::Peroxisomes were isolated from AS-30D hepatoma and compared to normal rat liver cells for the purpose of investigating the cholesterol accumulation in the hepatoma cells. Cholesterol was found to be approximately 10-fold higher relative to protein in AS-30D peroxisomes as compared to peroxisomes from normal liver. The...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lyons HT,Kharroubi A,Wolins N,Tenner S,Chanderbhan RF,Fiskum G,Donaldson RP

    更新日期:1991-03-01 00:00:00

  • Site-directed mutagenesis of cation coordinating residues in the gastric H,K-ATPase.

    abstract::Site-mutations were introduced into putative cation binding site 1 of the H,K-ATPase at glu-797, thr-825, and glu-938. The side chain oxygen of each was not essential but the mutations produced different activation and inhibition kinetics. Site mutations thr-825 (ala, leu) and glu-938 (ala, gln) modestly decreased the...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Rulli SJ,Louneva NM,Skripnikova EV,Rabon EC

    更新日期:2001-03-01 00:00:00

  • Regulation and intracellular localization of the biotin holocarboxylase synthetase of 3T3-L1 cells.

    abstract::A quantitative assay has been developed to measure holocarboxylase synthetase activity in cellular extracts. This assay was based on measuring the incorporation of [3H]biotin of high specific activity (4.3 Ci/mmol) into purified rat liver apopyruvate carboxylase. With this assay, holocarboxylase synthetase in 3T3-L1 m...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Chang HI,Cohen ND

    更新日期:1983-08-01 00:00:00

  • Bacterial expression of functional membrane-bound thromboxane synthase having intact sequence and truncated N-terminal hydrophobic segment.

    abstract::A full-length cDNA for human placental thromboxane synthase and a shortened cDNA lacking the sequence corresponding to the N-terminal 2-29 amino acids were expressed in Escherichia coli using a pCW expression vector. Both intact and truncated recombinant enzyme were found in the membrane fraction and were catalyticall...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Xia Z,Tai HH

    更新日期:1995-08-20 00:00:00

  • Purification and some properties of carbonic anhydrase from bovine skeletal muscle.

    abstract::Procedures for the purification of bovine muscle carbonic anhydrase (isoenzyme III) are described. The purified enzyme has a molecular weight near 29,000 and contains one Zn2+ ion per molecule. The sedimentation coefficient, s(0)20,w, is 2.8 X 10(-13) s, the isoelectric pH is 8.5, and A280(0.1%) = 2.07 cm-1. The CO2 h...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Engberg P,Millqvist E,Pohl G,Lindskog S

    更新日期:1985-09-01 00:00:00

  • Dietary control and tissue specific expression of branched-chain alpha-ketoacid dehydrogenase kinase.

    abstract::The branched-chain alpha-ketoacid dehydrogenase complex, catalyst for the rate-limiting step of branched-chain amino acid catabolism, is controlled by a highly specific protein kinase (branched-chain alpha-ketoacid dehydrogenase kinase) that associates tightly with the complex. The activity state (proportion of the en...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Popov KM,Zhao Y,Shimomura Y,Jaskiewicz J,Kedishvili NY,Irwin J,Goodwin GW,Harris RA

    更新日期:1995-01-10 00:00:00

  • Induction and superinduction of 2,3,7,8-tetrachlorodibenzo-rho-dioxin-inducible poly(ADP-ribose) polymerase: role of the aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator transcription activation domains and a labile transcription r

    abstract::The environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces a novel poly(ADP-ribose) polymerase (TiPARP). In this study, the signaling pathway of the induction was analyzed. Induction of TiPARP by TCDD occurs in both hepa1c1c7 cells and C57 mouse liver. Induction is concentration and time dependen...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ma Q

    更新日期:2002-08-15 00:00:00

  • Transcriptional regulation of rat liver glutathione S-transferase genes by phenobarbital and 3-methylcholanthrene.

    abstract::The relative rates of transcription of the rat liver glutathione S-transferase Ya-Yc and Yb genes were determined in purified liver nuclei isolated at different times after phenobarbital or 3-methylcholanthrene administration. The transcriptional rates of the Ya-Yc and Yb genes were elevated approximately fivefold 8 a...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ding VD,Pickett CB

    更新日期:1985-08-01 00:00:00

  • The photosynthetic deficiency due to puhC gene deletion in Rhodobacter capsulatus suggests a PuhC protein-dependent process of RC/LH1/PufX complex reorganization.

    abstract::Optimal photosynthetic reaction centre (RC) and core antenna (LH1) levels in the purple bacterium Rhodobacter capsulatus require the puhC gene. Deletion of puhC had little effect on RC and LH1 assembly individually, but significantly inhibited the photosynthetic growth of RC+ LH1- strains, suggesting that maximal RC c...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Aklujkar M,Prince RC,Beatty JT

    更新日期:2006-10-01 00:00:00

  • Identification of cis-regulatory elements and trans-acting proteins of the rat carbohydrate response element binding protein gene.

    abstract::Carbohydrate response element binding protein (ChREBP) is a transcription factor that activates liver glycolytic and lipogenetic enzyme genes in response to high carbohydrate diet. Here we report the transcriptional regulatory mechanisms for the rat ChREBP gene. Firstly, we determined the transcription initiation site...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Satoh S,Masatoshi S,Shou Z,Yamamoto T,Ishigure T,Semii A,Yamada K,Noguchi T

    更新日期:2007-05-01 00:00:00