Identification and characterization of protein kinase C-related enzymes in frog retina.

abstract：:Chloride ion is found to be an essential factor in photoreactivation of the oxygen-evolving center. Tris-washed, 2,6-dichlorophenol indophenol-treated grana are low in both Mn content and oxygen-evolving activity. These grana can restore high oxygen-evolving activity, however, by incorporating Mn2+ ion under weak ligh...

journal_title：Archives of biochemistry and biophysics

authors： Yamashita T,Ashizawa A

更新日期：1985-05-01 00:00:00

abstract：:Pseudonaja textilis, an Australian Elapid, is known to produce a highly toxic venom. Both protein profiling and N-terminal sequence analysis showed the presence of four new phospholipases A(2) in this venom. Besides being non-lethal, the phospholipase A(2) proteins were found to be moderately active enzymes and they s...

journal_title：Archives of biochemistry and biophysics

authors： Armugam A,Gong N,Li X,Siew PY,Chai SC,Nair R,Jeyaseelan K

更新日期：2004-01-01 00:00:00

abstract：:Activation of different forms of adenylate cyclases (AC) by forskolin and displacement of [14,15-3H]dihydroforskolin binding from membranes by forskolin in the absence or presence of specific stimulatory hormone and beta, gamma-imidoguanosine 5'-triphosphate (Gpp(NH)p) have been studied. These conditions have been use...

journal_title：Archives of biochemistry and biophysics

authors： Shi QH,Ruiz JA,Ho RJ

更新日期：1986-11-15 00:00:00

abstract：:The properties of porcine platelet acyltransferases which catalyze the incorporation of unsaturated fatty acids into the 2 positions of phospholipids were compared with those of porcine liver microsomes and rat liver microsomes. There were significant differences in the relative rates of incorporation of acyl groups i...

journal_title：Archives of biochemistry and biophysics

authors： Inoue M,Murase S,Okuyama H

更新日期：1984-05-15 00:00:00

abstract：:Reactive oxygen species (ROS) can be generated in experimental shock states through several different mechanisms. We measured ROS production in metabolically active liver mitochondria from rats rendered septic by cecal ligation and puncture. By polarography, the State 4 and State 3 respiration rates of liver mitochond...

journal_title：Archives of biochemistry and biophysics

authors： Taylor DE,Ghio AJ,Piantadosi CA

更新日期：1995-01-10 00:00:00

abstract：:The putrescine analogue 1,4-diamino-2-butanone (DAB) is highly toxic to various microorganisms, including Trypanosoma cruzi. Similar to other α-aminocarbonyl metabolites, DAB exhibits pro-oxidant properties. DAB undergoes metal-catalyzed oxidation yielding H(2)O(2), NH(4)(+) ion, and a highly toxic α-oxoaldehyde. In v...

journal_title：Archives of biochemistry and biophysics

authors： Soares CO,Colli W,Bechara EJ,Alves MJ

更新日期：2012-12-15 00:00:00

abstract：:Arachidonate 12-lipoxygenase (12-LO) from porcine leukocytes was expressed in insect cells using a baculovirus expression vector. The recombinant 12-LO was expressed as an N-terminal fusion protein with a 31-amino acid polypeptide carrying a six-histidine tag and an enterokinase cleavage site. Maximal intracellular en...

journal_title：Archives of biochemistry and biophysics

authors： Reddy RG,Yoshimoto T,Yamamoto S,Funk CD,Marnett LJ

更新日期：1994-07-01 00:00:00

abstract：:Several 2-halo-acyl-CoA derivatives have been synthesized to examine the interaction of these potential inhibitors of mitochondrial beta-oxidation with the purified medium chain acyl-CoA dehydrogenase from pig kidney. Racemic 2-bromo-, 2-chloro-, and 2-fluoro-octanoyl-CoA thioesters show 6.6, 33, and 3.5% of the activ...

journal_title：Archives of biochemistry and biophysics

authors： Cummings JG,Thorpe C

更新日期：1993-04-01 00:00:00

abstract：:Mitochondrial permeability transition (MPT) is a Ca(2+)-dependent, cyclosporine A-sensitive, non-selective inner membrane permeabilization induced by a wide range of agents or conditions, which has often been associated with necrotic or apoptotic cell death. When mitochondria isolated from livers of rats treated with ...

journal_title：Archives of biochemistry and biophysics

authors： Andreu GL,Delgado R,Velho JA,Curti C,Vercesi AE

更新日期：2005-07-15 00:00:00

abstract：:PCNA is well known as a component of DNA replication system and plays important roles in multiple cellular pathways in addition to replication and repair. In this work we have demonstrated the physical and functional interaction between tobacco PCNA and mungbean ddNTP-sensitive DNA polymerase which shares many physico...

journal_title：Archives of biochemistry and biophysics

authors： Roy S,Roy Choudhury S,Mukherjee SK,Sengupta DN

更新日期：2007-12-01 00:00:00

abstract：:The regulation of expression of hepatic iron-related proteins was examined during iron deficiency caused by scurvy in guinea pigs. Previous studies showed that some effects of scurvy, such as suppression of collagen gene expression, result from events associated with weight loss. During the initial phase of scurvy whe...

journal_title：Archives of biochemistry and biophysics

authors： Gosiewska A,Mahmoodian F,Peterkofsky B

更新日期：1996-01-15 00:00:00

abstract：:Bone-forming cells originate from distinct embryological layers, mesoderm (axial and appendicular bones) and ectoderm (precursor of neural crest cells, which mainly form facial bones). These cells will develop bones by two principal mechanisms: intramembranous and endochondral ossification. In both cases, condensation...

journal_title：Archives of biochemistry and biophysics

authors： Paiva KB,Granjeiro JM

更新日期：2014-11-01 00:00:00

abstract：:The ionization of 4-nitroimidazole to 4-nitroimidazolate was investigated as a function of ionic strength. The apparent pKa varies from 8.99 to 9.50 between 0.001 and 1.0 M ionic strength, respectively, at 25 degrees C. The ionic strength dependence of this ionization is anomalous. The binding of 4-nitroimidazole by h...

journal_title：Archives of biochemistry and biophysics

authors： Taylor KC,Vitello LB,Erman JE

更新日期：2000-10-15 00:00:00

abstract：:Differential light absorption spectra of the bilirubin-albumin 1:1 complex, obtained on addition of 20 different sulfonamides, differ with respect to shape and amplitude. This finding seems to indicate that the sulfonamide molecule is bound in direct touch with the bilirubin. The light absorption spectrum of bilirubin...

journal_title：Archives of biochemistry and biophysics

authors： Brodersen R,Knudsen A,Pedersen AO

更新日期：1987-02-01 00:00:00

abstract：:CYP119 from Sulfolobus solfataricus is the first well-characterized thermophilic cytochrome P450 enzyme. The endogenous substrate for this enzyme is not known but it hydroxylates lauric acid in a reaction supported by surrogate mesophilic electron donors. However, reconstitution of a high-temperature catalytic system ...

journal_title：Archives of biochemistry and biophysics

authors： Puchkaev AV,Ortiz de Montellano PR

更新日期：2005-02-01 00:00:00

abstract：:Human malic enzyme was studied by steady state kinetics, deuterium isotope effects, and 13C isotope effects with both the physiological dinucleotide cofactor and several alternate cofactors. The log V vs pH profile with NAD revealed two pK(a) values too close to be separately determined, but with an average value of 7...

journal_title：Archives of biochemistry and biophysics

authors： Rishavy MA,Yang Z,Tong L,Cleland WW

更新日期：2001-12-01 00:00:00

abstract：:The binding site for ATP to tubulin was established by use of the photoaffinity label [gamma-32P]N3ATP. Photolysis of the analog in the presence of tubulin resulted in covalent modification of the protein as revealed by autoradiography of electropherograms. Scanning the autoradiograms showed that the ATP analog was bo...

journal_title：Archives of biochemistry and biophysics

authors： Zabrecky JR,Cole RD

更新日期：1983-09-01 00:00:00

abstract：:The effect of copper sulfate (CuSO4) on both hepatic oxidative stress and heme oxygenase induction was studied. A strong increase in in vivo rat liver chemiluminescence was observed 1 h after Cu(II) administration. To evaluate liver antioxidant enzymatic defenses, superoxide dismutase, catalase, and glutathione peroxi...

journal_title：Archives of biochemistry and biophysics

authors： Ossola JO,Groppa MD,Tomaro ML

更新日期：1997-01-15 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) stimulates angiogenesis. Human hepatocellular carcinoma (HCC) is a VEGF-driven tumor often associated with chronic hepatitis B or C virus infection. The woodchuck is a well-characterized model of hepatitis B virus related HCC and a valuable tool for translational studies of no...

journal_title：Archives of biochemistry and biophysics

authors： Huang H,Salavaggione O,Rivera L,Mukherjee S,Brekken R,Tennant B,Iyer R,Adjei A

更新日期：2019-01-01 00:00:00

abstract：:We studied the kinetic properties of the membrane-bound human liver mitochondrial protoporphyrinogen oxidase. The activity was monitored by direct recording of protoporphyrin fluorescence appearance in the incubation medium without extraction or dilution. The human liver enzyme shows some different catalytic propertie...

journal_title：Archives of biochemistry and biophysics

authors： Camadro JM,Abraham NG,Levere RD

更新日期：1985-10-01 00:00:00

abstract：:Two complementary DNA (cDNA) clones (pTK-1 and -2) encoding two distinct isotypes of mouse Mg(2+)-dependent protein phosphatase beta (MPP beta-1 and -2, respectively) were isolated from a melanocyte cDNA library. Although mouse pTK-1 is orthologous to the rat cDNA (JW5) reported previously [Wenk, J., Trompeter, H.I., ...

journal_title：Archives of biochemistry and biophysics

authors： Terasawa T,Kobayashi T,Murakami T,Ohnishi M,Kato S,Tanaka O,Kondo H,Yamamoto H,Takeuchi T,Tamura S

更新日期：1993-12-01 00:00:00

abstract：:The possibility of a cooperative interaction between fat-soluble antioxidants is examined in a membrane model. A combination of beta-carotene and alpha-tocopherol results in an inhibition of lipid peroxidation significantly greater than the sum of the individual inhibitions. Our data provide the first evidence that be...

journal_title：Archives of biochemistry and biophysics

authors： Palozza P,Krinsky NI

更新日期：1992-08-15 00:00:00

abstract：:Interactions between loops 2, 2' and 4, known as the loop bundle, stabilize the active site of caspase-3. Loop 4 (L4) is of particular interest due to its location between the active site and the dimer interface. We have disrupted a salt bridge between K242 and E246 at the base of L4 to determine its role in overall c...

journal_title：Archives of biochemistry and biophysics

authors： Walters J,Swartz P,Mattos C,Clark AC

更新日期：2011-04-01 00:00:00

abstract：:The heme enzyme lignin peroxidase contains a unique Cbeta-hydroxylated tryptophan residue (Trp171) on the surface of the enzyme. Mutagenetic substitution of Trp171 abolishes completely the veratryl alcohol oxidation activity of the enzyme. This led us to surmise that Trp171 may be involved in electron transfer from na...

journal_title：Archives of biochemistry and biophysics

authors： Blodig W,Smith AT,Winterhalter K,Piontek K

更新日期：1999-10-01 00:00:00

abstract：:Differential scanning calorimetry measures the heat capacity of states and the excess heat associated with transitions that can be induced by temperature change. The integral of the excess heat capacity is the enthalpy for this process. Despite this potentially intimidating sounding physical chemistry background, DSC ...

journal_title：Archives of biochemistry and biophysics

authors： Johnson CM

更新日期：2013-03-01 00:00:00

abstract：:Topoisomerase II is a major molecular target for a number of DNA-binding anticancer drugs. In the present study, we report topoisomerase II inhibition and anticancer activity by four substituted ferrocene derivatives which do not bind to DNA. The first derivative, acetyl-substituted ferrocene (monoacetylferrocene), sh...

journal_title：Archives of biochemistry and biophysics

authors： Vashisht Gopal YN,Jayaraju D,Kondapi AK

更新日期：2000-04-01 00:00:00

abstract：:Autophagy, a ubiquitous degradation pathway, is important for the survival and homeostasis of cells. Previous studies have demonstrated the role of autophagy in host defense against bacterial infection, but the importance of autophagy in the intestinal epithelium for the regulation of bacterial infection has not been ...

journal_title：Archives of biochemistry and biophysics

authors： Inoue J,Nishiumi S,Fujishima Y,Masuda A,Shiomi H,Yamamoto K,Nishida M,Azuma T,Yoshida M

更新日期：2012-05-01 00:00:00

abstract：:Carbohydrate Binding Modules (CBMs) targeting cellulose, xylan and mannan have been reported, however, a CBM targeting rhamnogalacturonan I (RG I) has never been identified. We had studied earlier a rhamnogalacturonan lyase (CtRGL) from Clostridium thermocellum that was associated with a family 35 CBM, Rgl-CBM35. In t...

journal_title：Archives of biochemistry and biophysics

authors： Dhillon A,Sharma K,Rajulapati V,Goyal A

更新日期：2018-09-15 00:00:00

abstract：:Hepatocytes from fed rats were incubated for 120 min in the presence of alpha-D-[1,2-13C]glucose pentaacetate (1.7 mM), both D-[1,2-13C]glucose (1.7 mM) and acetate (8.5 mM), alpha-D-glucose penta[2-13C]acetate (1.7 mM), or D-[1,2-13C]glucose (8.3 mM). The amounts of 13C-enriched L-lactate and D-glucose and those of a...

journal_title：Archives of biochemistry and biophysics

authors： Malaisse WJ,Ladrière L,Kadiata MM,Verbruggen I,Willem R

更新日期：2000-09-01 00:00:00

abstract：:Plant 3-hydroxy-3-methylglutaryl-CoA reductase(HMGR; EC 1.1.1.34) and sucrose-phosphate synthase (SPS; EC 2.4.1.14) and synthetic peptides designed from the known phosphorylation sites of plant HMGR (SAMS*: KSHMKYNRSTKDVK), rat acetyl-CoA carboxylase (SAMS: HMRSAMSGLHLVKRR), spinach SPS (SP2: GRRJRRISSVEJJDKK), and sp...

journal_title：Archives of biochemistry and biophysics

authors： Toroser D,Huber SC

更新日期：1998-07-15 00:00:00