Identification and characterization of protein kinase C-related enzymes in frog retina.


:We isolated two types of protein kinase C-related enzymes (fPKC) in frog (Rana catesbeiana) retina. Three sequential steps of column chromatography of DEAE-cellulose, butyl-Toyopearl, and hydroxylapatite were carried out and resulted in the separation of two types of distinct Ca2+/phospholipid-dependent protein kinase activities. These proteins reacted with antibodies raised against synthetic peptides based on the predicted amino acid sequences of rat protein kinase C delta or zeta subspecies, and showed molecular masses of 90 and 75 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, respectively. Immunocytochemical analysis of 90 and 75K fPKC in frog retina revealed that the 75K fPKC was localized in the outer segment of cone cells and in the microvillar projections of Müller cells. These results suggested that fPKCs play differential roles on the photosignal transduction in frog retina.


Arch Biochem Biophys


Terasawa M,Hagiwara M,Hachiya T,Kobayashi R,Obata S,Wakabayashi T,Hidaka H




Has Abstract


1991-06-01 00:00:00














  • Characterization and partial amino acid sequence of human plasma glutathione peroxidase.

    abstract::Human plasma glutathione peroxidase was purified to homogeneity and partially sequenced. Overlapping peptide fragments from three endopeptidase digests permitted the determination of one sequence of 32 contiguous amino acids and one sequence of 23 contiguous amino acids. Five additional unique peptide sequences withou...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Esworthy RS,Chu FF,Paxton RJ,Akman S,Doroshow JH

    更新日期:1991-05-01 00:00:00

  • Interaction of the high-affinity inhibitor tetrahydro-dUMP with the allosteric enzyme deoxycytidylate aminohydrolase.

    abstract::Tetrahydro-dUMP, an analog of the putative transition state in aminohydrolysis of deoxycytidine monophosphate (dCMP) inhibits the allosteric enzyme deoxycytidylate aminohydrolase with high affinity. The inhibition is reversible, and its kinetics is consistent with the analog binding at the substrate site only to one a...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Nucci R,Febbraio F,Piccialli G,de Napoli L,Vaccaro C,Rossi M,Whitehead EP

    更新日期:1994-04-01 00:00:00

  • Insulin replacement therapy in diabetic rats using an osmotic pump normalizes expression of enzymes key to hepatic carbohydrate metabolism.

    abstract::Intensively treating type I diabetics with continuous subcutaneous insulin infusions or multiple daily insulin injections to normalize mean blood glucose concentrations significantly reduces the onset of secondary diabetic complications when compared to conventionally treated diabetics. Our studies focused on characte...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kramer KL,Giffin BF,Fox JW,Drake RL

    更新日期:1999-08-15 00:00:00

  • Alteration of product formation by directed mutagenesis and truncation of the multiple-product sesquiterpene synthases delta-selinene synthase and gamma-humulene synthase.

    abstract::Two recombinant sesquiterpene synthases from grand fir, delta-selinene synthase and gamma-humulene synthase, each produce more than 30 sesquiterpene olefins from the acyclic precursor farnesyl diphosphate. These enzymes contain a pair of DDxxD motifs, on opposite lips of the presumptive active site, which are thought ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Little DB,Croteau RB

    更新日期:2002-06-01 00:00:00

  • Luteolin enhances TRAIL sensitivity in non-small cell lung cancer cells through increasing DR5 expression and Drp1-mediated mitochondrial fission.

    abstract::Cancer cells exhibit extreme sensitivity to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) over normal cells, highlighting TRAIL's potential as a novel and effective cancer drug. However, the therapeutic effect of TRAIL is limited due to drug resistance. In the present study, we sought to investigate ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wu B,Xiong J,Zhou Y,Wu Y,Song Y,Wang N,Chen L,Zhang J

    更新日期:2020-10-15 00:00:00

  • Inhibitory effect of CP-25 on intimal formation and vascular hyperplasia via suppression of GRK2/ERK1/2/EVI1 signaling.

    abstract::Excessive proliferation, migration and dedifferentiation of vascular smooth muscle cells (VSMCs) are the center of intimal formation during in-stent restenosis and vein graft disease. Paeoniflorin-6'-O-benzene sulfonate (CP-25) is known to suppress inflammation and atherogenesis. However, the potential effect of CP-25...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Zhang J,Liu Y,Long M,Li J,Zhao W,Su Q

    更新日期:2020-11-15 00:00:00

  • Dose-dependent response of serum lutein and macular pigment optical density to supplementation with lutein esters.

    abstract::We conducted a study to determine the effect of different doses of a lutein supplement on serum lutein concentration and macular pigment optical density (MPOD). Lutein is one of the major components of human macular pigment. Eighty-seven subjects received daily doses of 5, 10, or 20 mg of lutein, or a placebo, over a ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 临床试验,杂志文章


    authors: Bone RA,Landrum JT

    更新日期:2010-12-01 00:00:00

  • Mangiferin, a natural occurring glucosyl xanthone, increases susceptibility of rat liver mitochondria to calcium-induced permeability transition.

    abstract::Mitochondrial permeability transition (MPT) is a Ca(2+)-dependent, cyclosporine A-sensitive, non-selective inner membrane permeabilization induced by a wide range of agents or conditions, which has often been associated with necrotic or apoptotic cell death. When mitochondria isolated from livers of rats treated with ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Andreu GL,Delgado R,Velho JA,Curti C,Vercesi AE

    更新日期:2005-07-15 00:00:00

  • Cytotoxicity of the redox cycling compound diquat in isolated hepatocytes: involvement of hydrogen peroxide and transition metals.

    abstract::Diquat is a hepatotoxin whose toxicity in vivo and in vitro is mediated by redox cycling and greatly enhanced by pretreatment with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), an inhibitor of glutathione reductase. The mechanism by which redox cycling mediates diquat cytotoxicity is unclear, however. Here, we have att...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sandy MS,Moldeus P,Ross D,Smith MT

    更新日期:1987-11-15 00:00:00

  • Characterization of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (AGEPC)-induced protein phosphorylation in rabbit platelets: inhibitory effects of AGEPC analogs.

    abstract::1-O-Alkyl-2-acetyl-sn-glycero-3-phosphocholine (AGEPC) induced phosphorylation of two proteins having molecular masses of approximately 20- and 40-kDa in washed rabbit platelets in a concentration- and time-dependent manner. Sequential stimulation with AGEPC did not induce additional protein phosphorylation, supportin...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sugatani J,Hanahan DJ

    更新日期:1986-05-01 00:00:00

  • The isolation and characterization of specific 3-methylcholanthrene-binding proteins from rat liver cytosol.

    abstract::The major proteins to which 3-methylcholanthrene specifically binds have been purified over 480-fold with a 45% yield compared to a rat liver 100,000g supernate. The procedure involved a batch ion-exchange technique together with hydrophobic gel filtration and chromatofocusing chromatography. The multiple, specific 3-...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Tierney B,Munzer S,Bresnick E

    更新日期:1983-09-01 00:00:00

  • Cold plasma inactivation of chronic wound bacteria.

    abstract::Cold plasma is partly ionized non-thermal plasma generated at atmospheric pressure. It has been recognized as an alternative approach in medicine for sterilization of wounds, promotion of wound healing, topical treatment of skin diseases with microbial involvement and treatment of cancer. Cold plasma used in wound the...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Mohd Nasir N,Lee BK,Yap SS,Thong KL,Yap SL

    更新日期:2016-09-01 00:00:00

  • Demonstration that menthofuran synthase of mint (Mentha) is a cytochrome P450 monooxygenase: cloning, functional expression, and characterization of the responsible gene.

    abstract::(+)-Menthofuran is an undesirable monoterpenoid component of peppermint (Mentha x piperita) essential oil that is derived from the alpha,beta-unsaturated ketone (+)-pulegone. Microsomal preparations, from the oil gland secretory cells of a high (+)-menthofuran-producing chemotype of Mentha pulegium, transform (+)-pule...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Bertea CM,Schalk M,Karp F,Maffei M,Croteau R

    更新日期:2001-06-15 00:00:00

  • Rapid turnover of endogenous endonuclease activity in thymocytes: effects of inhibitors of macromolecular synthesis.

    abstract::Previous work has shown that inhibitors of protein or mRNA synthesis block endonuclease activation in thymocytes undergoing programmed cell death. In the present study we used isolated nuclei to investigate the effects of cycloheximide and actinomycin D, inhibitors of protein and mRNA synthesis, respectively, on endog...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: McConkey DJ,Hartzell P,Orrenius S

    更新日期:1990-04-01 00:00:00

  • Crystal structure and immunogenicity of the class C acid phosphatase from Pasteurella multocida.

    abstract::Pasteurella multocida is a pathogen of veterinary and medical importance. Here, we report the 1.85Å resolution crystal structure of the class C acid phosphatase from this organism (denoted rPmCCAP). The structure shows that rPmCCAP exhibits the same haloacid dehalogenase fold and dimeric assembly as the class C enzyme...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Singh H,Malinski TJ,Reilly TJ,Henzl MT,Tanner JJ

    更新日期:2011-05-01 00:00:00

  • Expression and characterization of a functional canine variant of cytochrome b5 reductase.

    abstract::Cytochrome b5 reductase (cb5r), a member of the flavoprotein transhydrogenase family of oxidoreductase enzymes, catalyzes the transfer of reducing equivalents from the physiological electron donor, NADH, to two molecules of cytochrome b5. We have determined the correct nucleotide sequence for the putative full-length,...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Roma GW,Crowley LJ,Barber MJ

    更新日期:2006-08-01 00:00:00

  • Role of flavin in acetoin production by two bacterial pyruvate oxidases.

    abstract::Escherichia coli pyruvate oxidase (POXEC) requires FAD both for the oxidative decarboxylation of pyruvate to acetate and CO2 and for the formation of acetoin from pyruvate and acetaldehyde. Prior work has shown that the catalytic activity (kcat/Km) for POXEC in the oxidative reaction is stimulated approximately 450-fo...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Bertagnolli BL,Hager LP

    更新日期:1993-01-01 00:00:00

  • 1,4-Benzoquinone reductase from basidiomycete Phanerochaete chrysosporium: spectral and kinetic analysis.

    abstract::The reaction mechanism of a 1,4-benzoquinone reductase from the wood-rotting basidiomycete Phanerochaete chrysosporium was investigated. The native, oxidized, FMN-containing enzyme was reduced quantitatively by NADH and the resulting reduced enzyme was reoxidized in the presence of one equivalent of 2,6-di-methoxy-1,4...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Brock BJ,Gold MH

    更新日期:1996-07-01 00:00:00

  • sFRP-1 binds via its netrin-related motif to the N-module of thrombospondin-1 and blocks thrombospondin-1 stimulation of MDA-MB-231 breast carcinoma cell adhesion and migration.

    abstract::Secreted frizzled-related protein (sFRP)-1 is a Wnt antagonist that inhibits breast carcinoma cell motility, whereas the secreted glycoprotein thrombospondin-1 stimulates adhesion and motility of the same cells. We examined whether thrombospondin-1 and sFRP-1 interact directly or indirectly to modulate cell behavior. ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Martin-Manso G,Calzada MJ,Chuman Y,Sipes JM,Xavier CP,Wolf V,Kuznetsova SA,Rubin JS,Roberts DD

    更新日期:2011-05-15 00:00:00

  • Forms of adenylate cyclase, activation and/or potentiation by forskolin.

    abstract::Activation of different forms of adenylate cyclases (AC) by forskolin and displacement of [14,15-3H]dihydroforskolin binding from membranes by forskolin in the absence or presence of specific stimulatory hormone and beta, gamma-imidoguanosine 5'-triphosphate (Gpp(NH)p) have been studied. These conditions have been use...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Shi QH,Ruiz JA,Ho RJ

    更新日期:1986-11-15 00:00:00

  • Vanadyl and vanadate inhibit Ca2+ transport systems of the adipocyte plasma membrane and endoplasmic reticulum.

    abstract::Vanadate and vanadyl have many insulin-mimetic effects on cellular metabolism and also have been shown to alter cellular Ca2+ fluxes. In this report, vanadate and vanadyl, like insulin, are shown to inhibit the plasma membrane (Ca2+ + Mg2+)-ATPase/Ca2+ transport system as well as Ca2+ transport by endoplasmic reticulu...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Delfert DM,McDonald JM

    更新日期:1985-09-01 00:00:00

  • MicroRNA-342 targets Cofilin 1 to suppress the growth, migration and invasion of human breast cancer cells.

    abstract::MicroRNA-342-3p (miR-342) has been shown to act as a tumor-suppressor in different cancer types. However, the role and therapeutic implications of miR-342 via modulation of Cofilin 1 (CFL1) has not been studied in any type of cancer. Given the importance of Cofilin signalling in breast, this study was undertaken to ex...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Liu C,Xing H,Luo X,Wang Y

    更新日期:2020-07-15 00:00:00

  • Spontaneous porphyria of the Long-evans cinnamon rat: an animal model of Wilson's disease.

    abstract::To confirm and extend our previous microspectrophotometric observations of 30-week-old male Long-Evans Cinnamon (LEC) rats, an animal model of human Wilson's disease, we analyzed the porphyrin patterns of the organs, urine, and plasma of LEC rats. Abnormal accumulation of porphyrins, especially highly carboxylated por...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Nakayama K,Takasawa A,Terai I,Okui T,Ohyama T,Tamura M

    更新日期:2000-03-15 00:00:00

  • Nitroxyl-mediated disruption of thiol proteins: inhibition of the yeast transcription factor Ace1.

    abstract::Among the biologically and pharmacologically relevant nitrogen oxides, nitroxyl (HNO) remains one of the most poorly studied and least understood. Several previous reports indicate that thiols may be a primary target for the biological actions of HNO. However, the intimate details of the chemical interaction of HNO wi...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Cook NM,Shinyashiki M,Jackson MI,Leal FA,Fukuto JM

    更新日期:2003-02-01 00:00:00

  • The effect of Co(III)(NH3)4ATP on the kinetics of beef heart mitochondrial ATPase.

    abstract::Bidentate cobalt(III)tetraamine adenosine triphosphate [Co(NH3)4ATP] was investigated as an inhibitor of the beef heart mitochondrial F1-ATPase. The compound was found to have a mixed noncompetitive mechanism with a Ki of 0.4 mM and an alpha of 1.4 during ATP hydrolysis. Co(NH3)4ATP also noncompetitively inhibited ATP...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Steinke L,Schuster SM

    更新日期:1985-05-01 00:00:00

  • Brain mitochondrial cytochrome P-450scc: spectral and catalytic properties.

    abstract::The cytochrome P-450-dependent cholesterol side chain cleavage system of the brain has been studied using nonsynaptic mitochondria as the source of enzymatic activity. The system has been found to bind cholesterol and 11-deoxycorticosterone, producing type I difference spectra, whereas the binding of pregnenolone indu...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Walther B,Ghersi-Egea JF,Minn A,Siest G

    更新日期:1987-05-01 00:00:00

  • Toxicity of S-pentachlorobutadienyl-L-cysteine studied with isolated rat renal cortical mitochondria.

    abstract::The subcellular mechanism of alkenyl halide S-conjugate-induced nephrotoxicity was studied in mitochondria isolated from rat kidney cortex in vitro using the cysteine conjugate of hexachloro-1,3-butadiene, i.e., S-pentachlorobutadienyl-L-cysteine (PCBC) as a model substrate. Respiring mitochondria exposed to various c...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wallin A,Jones TW,Vercesi AE,Cotgreave I,Ormstad K,Orrenius S

    更新日期:1987-11-01 00:00:00

  • The molecular weight and oligomerization of rabbit thrombomodulin as assessed by sedimentation equilibrium.

    abstract::Lubrol-solubilized rabbit thrombomodulin has been examined by equilibrium sedimentation in buffers that include sufficient D2O to make the detergent neutrally buoyant. Data were acquired at rotor speeds from 12,000 to 28,000 rpm from two thrombomodulin preparations, at protein concentrations from 0.01 to 0.07%, and in...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Winnard PT,Esmon CT,Laue TM

    更新日期:1989-02-15 00:00:00

  • Alkaline treatment has contrasting effects on the structure of deglycosylated and glycosylated forms of glucose oxidase.

    abstract::X-ray crystallographic studies on glucose oxidase showed a strong interaction between carbohydrate and protein moieties of the glycoprotein. However, experimental studies under physiological conditions reported no influence of carbohydrate moiety on the structural and functional properties of glucose oxidase. In order...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Akhtar MS,Bhakuni V

    更新日期:2003-05-15 00:00:00

  • Reaction of uric acid with peroxynitrite and implications for the mechanism of neuroprotection by uric acid.

    abstract::Peroxynitrite, a biological oxidant formed from the reaction of nitric oxide with the superoxide radical, is associated with many pathologies, including neurodegenerative diseases, such as multiple sclerosis (MS). Gout (hyperuricemic) and MS are almost mutually exclusive, and uric acid has therapeutic effects in mice ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Squadrito GL,Cueto R,Splenser AE,Valavanidis A,Zhang H,Uppu RM,Pryor WA

    更新日期:2000-04-15 00:00:00