当前位置： SCI文献检索 > CANCER期刊下所有文献 > Pathomorphologic evaluation of pulmonary radiofrequency ablation: proof of cell death is characterized by DNA fragmentation and apoptotic bodies.

Pathomorphologic evaluation of pulmonary radiofrequency ablation: proof of cell death is characterized by DNA fragmentation and apoptotic bodies.

Abstract：

BACKGROUND:Radiofrequency (RF) ablation is an increasingly applied technique. Promising results of hepatic RF ablation raised expectations of its capabilities for treatment of primary and secondary lung tumors. Because of different thermal and electrical properties of lung tissue, compared with liver tissue, a simple analogy of tissue response is not possible. The authors aimed to evaluate the effectiveness of image-guided pulmonary RF ablation and to characterize pathomorphology of tissue response. METHODS:RF ablations of 11 pulmonary malignancies in 9 patients were performed under computed tomography (CT)-guidance. Three days after RF ablation, surgical resection was performed followed by pathologic examination. Specimens were evaluated macroscopically, histologically by hematoxylin and eosin (H & E) staining, terminal deoxy-nucleotidyl transferase-mediated nick end-labeling (TUNEL), and electron microscopy. RESULTS:Tumor tissues and adjacent lung tissues were characterized by double-strand fragmentation as determined by TUNEL. Ultrastructurally apoptotic bodies were found, indicating apoptotic cells. Criteria for tissue necrosis were not fulfilled by standard histological staining (H & E), showing preserved tissue architecture and only few microscopic cellular details suggestive of tumor regression. Because of DNA fragmentation, as determined by TUNEL and results from electron microscopy, the authors confirmed the tumor tissue to be completely ablated in 10 (90.9%) cases. However, in 2 cases, a safety margin was absent. CONCLUSIONS:CT-guided pulmonary RF ablation of pulmonary malignancies is a locally effective treatment. Three days after RF ablation, tumor tissue seemed to be thermally fixed still showing characteristics of vital tumor tissue in standard histological staining; however the tissue proved to be in regression toward coagulative necrosis verified ultrastructurally and by TUNEL.

journal_name

Cancer

journal_title

Cancer

authors

Clasen S,Krober SM,Kosan B,Aebert H,Fend F,Bomches A,Claussen CD,Pereira PL

doi

10.1002/cncr.23882

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

3121-9

issue

eissn

0008-543X

issn

1097-0142

journal_volume

113

pub_type

临床试验,杂志文章

在线工具

Pathomorphologic evaluation of pulmonary radiofrequency ablation: proof of cell death is characterized by DNA fragmentation and apoptotic bodies.