Pathomorphologic evaluation of pulmonary radiofrequency ablation: proof of cell death is characterized by DNA fragmentation and apoptotic bodies.


BACKGROUND:Radiofrequency (RF) ablation is an increasingly applied technique. Promising results of hepatic RF ablation raised expectations of its capabilities for treatment of primary and secondary lung tumors. Because of different thermal and electrical properties of lung tissue, compared with liver tissue, a simple analogy of tissue response is not possible. The authors aimed to evaluate the effectiveness of image-guided pulmonary RF ablation and to characterize pathomorphology of tissue response. METHODS:RF ablations of 11 pulmonary malignancies in 9 patients were performed under computed tomography (CT)-guidance. Three days after RF ablation, surgical resection was performed followed by pathologic examination. Specimens were evaluated macroscopically, histologically by hematoxylin and eosin (H & E) staining, terminal deoxy-nucleotidyl transferase-mediated nick end-labeling (TUNEL), and electron microscopy. RESULTS:Tumor tissues and adjacent lung tissues were characterized by double-strand fragmentation as determined by TUNEL. Ultrastructurally apoptotic bodies were found, indicating apoptotic cells. Criteria for tissue necrosis were not fulfilled by standard histological staining (H & E), showing preserved tissue architecture and only few microscopic cellular details suggestive of tumor regression. Because of DNA fragmentation, as determined by TUNEL and results from electron microscopy, the authors confirmed the tumor tissue to be completely ablated in 10 (90.9%) cases. However, in 2 cases, a safety margin was absent. CONCLUSIONS:CT-guided pulmonary RF ablation of pulmonary malignancies is a locally effective treatment. Three days after RF ablation, tumor tissue seemed to be thermally fixed still showing characteristics of vital tumor tissue in standard histological staining; however the tissue proved to be in regression toward coagulative necrosis verified ultrastructurally and by TUNEL.






Clasen S,Krober SM,Kosan B,Aebert H,Fend F,Bomches A,Claussen CD,Pereira PL




Has Abstract


2008-12-01 00:00:00














  • A study of the histopathogenesis of carcinoid tumors of the small intestine and appendix.

    abstract::A specimen from the small intestine with multiple (three) classic carcinoid tumors and one appendiceal carcinoid tumor displaying argentaffinity, argyrophilia (Grimelius stain), and serotonin and neuron specific enolase immunoreactivity was examined by light microscopy with regard to the tumor cell histopathogenesis. ...


    pub_type: 杂志文章


    authors: Lundqvist M,Wilander E

    更新日期:1987-07-15 00:00:00

  • Provocative questions in osteosarcoma basic and translational biology: A report from the Children's Oncology Group.

    abstract::Patients who are diagnosed with osteosarcoma (OS) today receive the same therapy that patients have received over the last 4 decades. Extensive efforts to identify more effective or less toxic regimens have proved disappointing. As we enter a postgenomic era in which we now recognize OS not as a cancer of mutations bu...


    pub_type: 杂志文章,评审


    authors: Roberts RD,Lizardo MM,Reed DR,Hingorani P,Glover J,Allen-Rhoades W,Fan T,Khanna C,Sweet-Cordero EA,Cash T,Bishop MW,Hegde M,Sertil AR,Koelsche C,Mirabello L,Malkin D,Sorensen PH,Meltzer PS,Janeway KA,Gorlick R,Cro

    更新日期:2019-10-15 00:00:00

  • Kaposi's sarcoma in two lymphoma patients with hepatitis B and other infections.

    abstract::Kaposi's sarcoma developed in two lymphoma patients previously treated by chemotherapy and chemoirradiation. The histologic picture in the two cases showed a non-Hodgkin's lymphoma with multifocal epithelioid histiocytic reaction. Both patients had Hepatitis-B surface antigen and other infections due to herpes and str...


    pub_type: 杂志文章


    authors: Berrebi A,Shtalrid M,Hurwitz N,Frumkin A,Schattner A

    更新日期:1984-08-15 00:00:00

  • Low serum albumin levels and liver metastasis are powerful prognostic markers for survival in patients with carcinomas of unknown primary site.

    abstract:BACKGROUND:The authors investigated how lymphopenia and low serum albumin levels correlate with the prognosis of patients with carcinoma of unknown primary (CUP). METHODS:Univariate and multivariate prognostic factor analyses were conducted in a population of 317 consecutive patients with CUP who were evaluated at the...


    pub_type: 杂志文章


    authors: Seve P,Ray-Coquard I,Trillet-Lenoir V,Sawyer M,Hanson J,Broussolle C,Negrier S,Dumontet C,Mackey JR

    更新日期:2006-12-01 00:00:00

  • Mediterranean Kaposi's sarcoma in the elderly. A randomized study of oral etoposide versus vinblastine.

    abstract:BACKGROUND:This Phase III trial was performed to compare the roles of oral etoposide and intravenous (i.v.) vinblastine in the treatment of Mediterranean Kaposi's Sarcoma (MEKS) in elderly patients with severe disease (Stages II, Ac/B, III, and IV). PATIENTS AND METHODS:Sixty-five patients were randomized to receive e...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Brambilla L,Labianca R,Boneschi V,Fossati S,Dallavalle G,Finzi AF,Luporini G

    更新日期:1994-11-15 00:00:00

  • CT-guided percutaneous microwave ablation of adrenal malignant carcinoma: preliminary results.

    abstract:BACKGROUND:Microwave ablation has recently been developed as a safe and effective treatment for a variety of tumors. The authors evaluated the safety and efficacy of computed tomography (CT)-guided percutaneous microwave ablation of adrenal malignant tumors. METHODS:Nine patients between 41 and 83 years of age (averag...


    pub_type: 杂志文章


    authors: Li X,Fan W,Zhang L,Zhao M,Huang Z,Li W,Gu Y,Gao F,Huang J,Li C,Zhang F,Wu P

    更新日期:2011-11-15 00:00:00

  • Dose-ranging antiemetic evaluation of the serotonin antagonist tropisetron in patients receiving anti-cancer chemotherapy.

    abstract:BACKGROUND:Tropisetron (ICS 205-930) antagonizes the serotonin type 3 receptor and has antiemetic activity in animals given cisplatin. Its mean serum half-life in 11.1 hours. METHODS:In this dose-ranging trial, 22 patients undergoing anti-cancer chemotherapy received 24 courses of a single intravenous infusion of trop...


    pub_type: 杂志文章


    authors: Pisters KM,Kris MG,Tyson LB,Clark RA,Gralla RJ

    更新日期:1993-01-01 00:00:00

  • Humoral hypercalcemia in patients with colorectal carcinoma: report of two cases and review of the literature.

    abstract:BACKGROUND:Humoral hypercalcemia rarely is associated with colorectal carcinoma; to the authors' knowledge only nine cases have been reported to date. METHODS:Two cases of advanced colorectal carcinoma with humoral hypercalcemia of malignancy (HHM) are presented. RESULTS:The two patients had severe hypercalcemia with...


    pub_type: 杂志文章,评审


    authors: Lortholary AH,Cadeau SD,Bertrand GM,Guerin-Meyer VI,Gamelin EC,Audran MJ

    更新日期:1999-12-01 00:00:00

  • Adult acute leukemia. Aggressive treatment improves survival.

    abstract::Although improvements in the management of adults with acute leukemia have lagged behind those seen in the treatment of children, there are clear reasons for optimism. Combination chemotherapy for acute myeloblastic leukemia (AML) and acute lymphoblastic leukemia (ALL) in adults results in complete remissions in 50-85...


    pub_type: 杂志文章


    authors: Eyre HJ,Ward JH,Priebat DA

    更新日期:1983-06-15 00:00:00

  • Extremity and trunk desmoid tumors: a multifactorial analysis of outcome.

    abstract:BACKGROUND:The natural history of desmoid tumors remains an enigma. Previous reports attempting to identify their biology have included recurrent and primary tumors as well as tumors from both intra- and extra-abdominal sites. The purpose of this study was to analyze patients with primary extremity and trunk desmoid tu...


    pub_type: 杂志文章


    authors: Merchant NB,Lewis JJ,Woodruff JM,Leung DH,Brennan MF

    更新日期:1999-11-15 00:00:00

  • Patterns of relapse of N2 nonsmall-cell lung carcinoma patients treated with preoperative chemotherapy: should prophylactic cranial irradiation be reconsidered?

    abstract:BACKGROUND:Although it induces a relevant reduction in the risk of both visceral metastases and locoregional recurrences, the combination of chemotherapy and surgery only marginally improves the survival of patients with Stage IIIA(N2) (International Union Against Cancer staging and classification system) nonsmall-cell...


    pub_type: 杂志文章


    authors: Andre F,Grunenwald D,Pujol JL,Girard P,Dujon A,Brouchet L,Brichon PY,Westeel V,Le Chevalier T

    更新日期:2001-06-15 00:00:00

  • Nutrition education of the cancer patient and family. Myths and realities.

    abstract::Common myths about nutrition education and care of cancer patients are debunked and realities are discussed. First, frequently held misconceptions of nonspecialized health professionals are considered. These include the myths that diet change in the population will be rapid now that dietary guidelines to prevent cance...


    pub_type: 杂志文章


    authors: Dwyer J

    更新日期:1986-10-15 00:00:00

  • Improved detection of focal lesions with computerized tomographic examination of the liver using ethiodized oil emulsion (EOE-13) liver contrast.

    abstract::A prospective study of computerized tomography (CT) of the liver using a liver contrast agent compared to conventional CT was undertaken. CT scanning without contrast agents (NC-CT), CT with water-soluble contrast (WSC-CT), and CT with ethiodized oil emulsion (EOE-CT) were performed on patients with possible or probab...


    pub_type: 杂志文章


    authors: Sugarbaker PH,Vermess M,Doppman JL,Miller DL,Simon R

    更新日期:1984-10-15 00:00:00

  • Prognostic factors associated with progression of smoldering multiple myeloma to symptomatic form.

    abstract:BACKGROUND:Smoldering multiple myeloma (SMM) presents a high risk of progression to symptomatic MM (sy-MM). Herein, we analyzed some predictors of development of sy-MM. In 144 patients with SMM, we also compared the risk of progression predicted by bone marrow plasma cell (BMPC) involvement on the bone marrow biopsy (B...


    pub_type: 杂志文章


    authors: Rago A,Grammatico S,Za T,Levi A,Mecarocci S,Siniscalchi A,De Rosa L,Felici S,Bongarzoni V,Piccioni AL,La Verde G,Pisani F,Franceschini L,Paviglianiti AL,Caravita T,Petrucci MT,De Stefano V,Cimino G,Multiple Myeloma GI

    更新日期:2012-11-15 00:00:00

  • Dose-escalation study of vemurafenib with sorafenib or crizotinib in patients with BRAF-mutated advanced cancers.

    abstract:BACKGROUND:BRAF inhibitors are effective in melanoma and other cancers with BRAF mutations; however, patients ultimately develop therapeutic resistance through the activation of alternative signaling pathways such as RAF/RAS or MET. The authors hypothesized that combining the BRAF inhibitor vemurafenib with either the ...


    pub_type: 杂志文章


    authors: Janku F,Sakamuri D,Kato S,Huang HJ,Call SG,Naing A,Holley VR,Patel SP,Amaria RN,Falchook GS,Piha-Paul SA,Zinner RG,Tsimberidou AM,Hong DS,Meric-Bernstam F

    更新日期:2021-02-01 00:00:00

  • Primary lymphoma of bone: the relationship of morphologic diversity to clinical behavior.

    abstract::Since primary lymphoma of bone (PLB) exhibits morphologic diversity and variability in individual survival, we analyzed the relationship between histopathologic features and biological behavior in 33 patients treated at the Massachusetts General Hospital. Three major histologic subgroups were identified, based on a va...


    pub_type: 杂志文章


    authors: Dosoretz DE,Raymond AK,Murphy GF,Doppke KP,Schiller AL,Wang CC,Suit HD

    更新日期:1982-09-01 00:00:00

  • Rhabdomyosarcoma of the oral and paraoral region.

    abstract::Oral rhabdomyosarcoma (RMS) was studied by analysis of eight such cases which presented over a 25-year period. Rhabdomyosarcoma was the fourth most common oral sarcoma (7.5%) from this period after osteosarcoma (32%), fibrosarcoma (19%), and chondrosarcoma (9%). In patients younger than 20 years, RMS was the second mo...


    pub_type: 杂志文章,评审


    authors: Peters E,Cohen M,Altini M,Murray J

    更新日期:1989-03-01 00:00:00

  • Improved control of cisplatin-induced emesis with high-dose metoclopramide and with combinations of metoclopramide, dexamethasone, and diphenhydramine. Results of consecutive trials in 255 patients.

    abstract::A series of consecutive trials were undertaken to determine whether higher doses of intravenous metoclopramide and combinations of metoclopramide, dexamethasone, and diphenhydramine would improve antiemetic control or decrease treatment-related side effects in patients receiving cisplatin at 120 mg/m2. Metoclopramide ...


    pub_type: 临床试验,杂志文章


    authors: Kris MG,Gralla RJ,Tyson LB,Clark RA,Kelsen DP,Reilly LK,Groshen S,Bosl GJ,Kalman LA

    更新日期:1985-02-01 00:00:00

  • Low participation rates and disparities in participation in interventional clinical trials for myelodysplastic syndromes.

    abstract:BACKGROUND:The development of novel therapies for the myelodysplastic syndromes (MDS) is hampered by inadequate trial recruitment. Factors contributing to low trial accrual are incompletely understood. METHODS:This study analyzed a pooled patient database from institutions of the US MDS Clinical Research Consortium to...


    pub_type: 杂志文章


    authors: Brierley CK,Zabor EC,Komrokji RS,DeZern AE,Roboz GJ,Brunner AM,Stone RM,Sekeres MA,Steensma DP

    更新日期:2020-11-01 00:00:00

  • Utility of prospective pathologic evaluation to inform clinical genetic testing for hereditary leiomyomatosis and renal cell carcinoma.

    abstract:BACKGROUND:Patients with hereditary leiomyomatosis and renal cell carcinoma (HLRCC) resulting from fumarate hydratase (FH) mutations may present with skin, uterine, and renal tumors, with each having unique pathologic features. This study investigated the association between prospectively identified suspicious patholog...


    pub_type: 杂志文章


    authors: Kopp RP,Stratton KL,Glogowski E,Schrader KA,Rau-Murthy R,Russo P,Coleman JA,Offit K

    更新日期:2017-07-01 00:00:00

  • Interaction of molecular alterations with immune response in melanoma.

    abstract::Major advances have been made in melanoma treatment with the use of molecularly targeted therapies and immunotherapies, and numerous regimens are now approved by the US Food and Drug Administration for patients with stage IV disease. However, therapeutic resistance remains an issue to both classes of agents, and relia...


    pub_type: 杂志文章,评审


    authors: Szczepaniak Sloane RA,Gopalakrishnan V,Reddy SM,Zhang X,Reuben A,Wargo JA

    更新日期:2017-06-01 00:00:00

  • Multimodality therapy for patients with clinical Stage I and II malignant mixed Müllerian tumors of the uterus.

    abstract:BACKGROUND:The role of adjuvant therapy in the management of patients with malignant mixed Müllerian tumors (MMMT) of the uterus has not been defined. The outcome of planned multimodality therapy for patients with apparent early stage disease was assessed. METHODS:A pilot study was performed on 38 patients with clinic...


    pub_type: 临床试验,杂志文章


    authors: Manolitsas TP,Wain GV,Williams KE,Freidlander M,Hacker NF

    更新日期:2001-04-15 00:00:00

  • Expression of receptor tyrosine kinases epidermal growth factor receptor and HER-2/neu in synovial sarcoma.

    abstract:BACKGROUND:Synovial sarcomas are high-grade soft tissue neoplasms often characterized by a biphasic spindle and epithelioid cell morphology. The majority of synovial sarcomas harbor a specific chromosomal translocation in which the proximal portion of the SYT gene at chromosome 18q11 is fused to the distal portion of o...


    pub_type: 杂志文章


    authors: Thomas DG,Giordano TJ,Sanders D,Biermann S,Sondak VK,Trent JC,Yu D,Pollock RE,Baker L

    更新日期:2005-02-15 00:00:00

  • Focal heating of V-2 tumors with a hybrid radiofrequency applicator.

    abstract::A hybrid radiofrequency heating system previously reported to produce highly focused heating patterns at less than or equal to 10 cm depth in phantom models was utilized to selectively heat hypervascular, heat resistant rabbit V-2 tumors implanted in the left hindlimb. Spheroidal heating patterns 3 to 4 cm in diameter...


    pub_type: 杂志文章


    authors: Boddie AW Jr,Merritt DR,Yamanashi W,Frazer JW,McBride CM,Martin RG

    更新日期:1985-03-15 00:00:00

  • Primary liver cancer incidence among American Indians and Alaska Natives, US, 1999-2004.

    abstract:BACKGROUND:American Indians and Alaska Natives (AI/AN) experience higher morbidity and mortality from primary liver cancer than other United States (US) populations, but racial misclassification in medical records results in underestimates of disease burden. METHODS:To reduce misclassification, National Program of Can...


    pub_type: 杂志文章


    authors: Jim MA,Perdue DG,Richardson LC,Espey DK,Redd JT,Martin HJ,Kwong SL,Kelly JJ,Henderson JA,Ahmed F

    更新日期:2008-09-01 00:00:00

  • Patterns of locoregional treatment for nonmetastatic breast cancer by patient and health system factors.

    abstract:BACKGROUND:The purpose of this study was to examine local definitive therapy for nonmetastatic breast cancer with the Patterns of Care Breast and Prostate Cancer (POCBP) study of the National Program of Cancer Registries (Centers for Disease Control and Prevention). METHODS:POCBP medical record data were re-abstracted...


    pub_type: 杂志文章


    authors: Anderson RT,Morris CR,Kimmick G,Trentham-Dietz A,Camacho F,Wu XC,Sabatino SA,Fleming ST,Lipscomb J

    更新日期:2015-03-01 00:00:00

  • Low-dose continuous infusion 5-fluorouracil. Evaluation in advanced breast carcinoma.

    abstract::Low-dose continuous infusion 5-fluorouracil (LDCI-FU) was administered to 28 women with advanced breast carcinoma. Daily doses ranged from 175 to 250 mg/m2. The LDCI-FU was delivered continuously until the appearance of toxicity and was reinstituted at a 20% dose reduction after toxicity completely resolved. Patients ...


    pub_type: 杂志文章


    authors: Huan S,Pazdur R,Singhakowinta A,Samal B,Vaitkevicius VK

    更新日期:1989-02-01 00:00:00

  • An aggressive approach to marginally resectable lung cancer.

    abstract::Between July 1968 and December 1974, 53 patients with lung cancer were planned for preoperative irradiation and surgery. All patients were considered clinically marginally resectable because of advanced local disease, 4 Stage II patients, with limited pulmonary reserve and 49 Stage III patients. Most patients received...


    pub_type: 杂志文章


    authors: Sherman DM,Neptune W,Weichselbaum R,Order SE,Piro AJ

    更新日期:1978-05-01 00:00:00

  • Axillary lymph nodes and breast cancer: a review.

    abstract:BACKGROUND:The value of surgical staging and treatment of the axillary lymph nodes with either surgery or radiotherapy in the initial management of patients with Stage I or II invasive breast cancer is controversial. METHODS:A review of retrospective and prospective clinical studies was performed to assess the risks o...


    pub_type: 杂志文章,评审


    authors: Recht A,Houlihan MJ

    更新日期:1995-11-01 00:00:00

  • Chemotherapy versus chemotherapy plus hormonotherapy in postmenopausal advanced breast cancer patients. A randomized trial.

    abstract::One hundred seventeen postmenopausal advanced breast cancer patients previously untreated with chemotherapy were randomized to receive: cyclophosphamide, methotrexate, and 5-fluorouracil (CMF), CMF and tamoxifen (TMX), and CMF and medroxyprogesterone (MAP). Treatments B and C induced a greater proportion of responses ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Viladiu P,Alonso MC,Avella A,Beltrán M,Borrás J,Ojeda B,Bosch FX

    更新日期:1985-12-15 00:00:00