A comparison of three cell types as potential candidates for intervertebral disc therapy: annulus fibrosus cells, chondrocytes, and bone marrow derived cells.


OBJECTIVES:Candidate cell types for disc cell transplantation therapy include anulus fibrosus (AF) cells, chondrocytes, and bone marrow derived cells (BMDCs). We compared the disc matrix production in these three types of cells, before and after stimulation with rhBMP-2. There is no study extant that compares these three cell types to determine the best candidate for the disc cell therapy. METHODS:AF cells, chondrocytes, and BMDCs (iliac crest and femur) were isolated and grown in monolayer. They were treated for 3 days with rhBMP-2. After 3 days, proteoglycan (sGAG) content in the media was quantified. The results were normalized by cell numbers. The mRNA expression of aggrecan, type I collagen, and type II collagen was measured using real-time PCR. Each cell type was also cultured in chamber slides and immunostained for aggrecan, type I collagen, and type II collagen after 3 days of treatment with rhBMP-2. RESULTS:(1) Without rhBMP-2 the chondrocytes produced more proteoglycan (sGAG) as compared to the other two cell types (AF cells and BMDCs). After stimulation with rhBMP-2 the chondrocytes produce even more proteoglycan than the other two cell types. (2) As compared to the other two cell types, in terms of mRNA expression, the chondrocytes expressed more aggrecan, type I collagen, and type II collagen before stimulation with rhBMP-2. After rhBMP-2 stimulation, the chondrocytes expressed even more aggrecan, type I collagen, and type II collagen in proportion to the concentration of rhBMP-2. For the BMDCs there were no changes in type I and II collagen. (3) rhBMP-2 stimulation produced increases in the protein levels of aggrecan, type I and II collagen in all three types of cells. CONCLUSIONS:On balance, according to these results, it would seem that chondrocytes are the best candidate for the disc cell therapy.


Joint Bone Spine


Joint bone spine


Kuh SU,Zhu Y,Li J,Tsai KJ,Fei Q,Hutton WC,Yoon TS




Has Abstract


2009-01-01 00:00:00














  • Femoral and lower costosternal junctions' osteitis in an adult with SAPHO syndrome: An unusual presentation.

    abstract::The combination of synovitis, acne, pustulosis, hyperostosis and aseptic osteitis is known as SAPHO syndrome. Osteitis involves the anterior chest, particularly the sternoclavicular and upper costosternal junctions. Diagnosis is difficult when there are no typical skin and bone lesions and differential diagnosis inclu...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Mylona E,Golfinopoulou S,Peppas C,Fanourgiakis P,Skoutelis A

    更新日期:2008-05-01 00:00:00

  • Aortic involvement in relapsing polychondritis.

    abstract:OBJECTIVE:To assess prevalence of aortic involvement in relapsing polychondritis (RP) patients; to evaluate clinical features and long-term outcome of RP patients exhibiting aortitis, aortic ectasia and/or aneurysm. METHODS:One hundred and seventy-two RP patients underwent aortic computed tomography (CT)-scan; they we...

    journal_title:Joint bone spine

    pub_type: 杂志文章,多中心研究


    authors: Le Besnerais M,Arnaud L,Boutémy J,Bienvenu B,Lévesque H,Amoura Z,Marie I

    更新日期:2018-05-01 00:00:00

  • Entheses ultrasound assessment in primary Sjogren's syndrome.

    abstract:OBJECTIVE:Musculoskeletal pain is a common complaint among patients with primary Sjogren's syndrome (pSS). Joints clinical examination is oftenly normal. A periarticular origin of this pain may be possible. Since clinical examination lacks sensitivity and precision, the use of musculoskeletal ultrasound (US) is more in...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Ben Abdelghani K,Miladi S,Chammekhi M,Fazaa A,Sallemi M,Ouenniche K,Souabni L,Kassab S,Chekili S,Zakraoui L,Laatar A

    更新日期:2020-07-01 00:00:00

  • Physical activity in patients with rheumatoid arthritis.

    abstract:INTRODUCTION:Rheumatoid arthritis (RA) is the most common chronic inflammatory joint disease and is associated with an excess risk of cardiovascular disease. For the general population, the World Health Organization has issued detailed recommendations on the type of physical activity appropriate for decreasing the card...

    journal_title:Joint bone spine

    pub_type: 杂志文章,评审


    authors: Verhoeven F,Tordi N,Prati C,Demougeot C,Mougin F,Wendling D

    更新日期:2016-05-01 00:00:00

  • Androgens and bone metabolism.

    abstract::The gradual reductions in bone mass and skeletal calcium density seen throughout adulthood occur in parallel with changes in the production of bioactive sex hormones in both men and women. The long-held belief that osteoporosis is dependent on androgens in men and estrogens in women has been challenged by recent repor...

    journal_title:Joint bone spine

    pub_type: 杂志文章,评审


    authors: Alexandre C

    更新日期:2005-05-01 00:00:00

  • Early diagnosis and management are crucial in spondyloarthritis.

    abstract::Spondyloarthritis raises considerable diagnostic challenges. The mean time from symptom onset to the definitive diagnosis was 8 years in classical studies. This diagnostic delay has a deleterious impact not only for the patient, but also potentially on the course of the disease and therefore on its social and economic...

    journal_title:Joint bone spine

    pub_type: 杂志文章,评审


    authors: Wendling D,Claudepierre P,Prati C

    更新日期:2013-12-01 00:00:00

  • Osteitis pubis after Marshall-Marchetti-Krantz urethropexy.

    abstract::We report a case of osteitis pubis after Marshall-Marchetti-Krantz urethropexy. There are several theories on the pathogenesis of the postoperative osteitis pubis and inconsistent results with a wide variety of treatments. Also, osteomyelitis and osteitis pubis can have identical symptoms. In our case, the use of a Mi...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Garcia-Porrua C,Picallo JA,Gonzalez-Gay MA

    更新日期:2003-02-01 00:00:00

  • Toxic effects of nonsteroidal antiinflammatory drugs on the small bowel, colon, and rectum.

    abstract::The gastrointestinal toxicity of conventional nonsteroidal antiinflammatory drugs (NSAIDs) is not confined to the stomach and proximal duodenum but extends also to the rest of the small bowel, colon, and rectum. Long-term NSAID therapy usually induces clinically silent enteropathy characterized by increased intestinal...

    journal_title:Joint bone spine

    pub_type: 杂志文章,评审


    authors: Thiéfin G,Beaugerie L

    更新日期:2005-07-01 00:00:00

  • Defining treatment failure in severe osteoporosis.

    abstract::The management of postmenopausal osteoporosis has benefited from the recent introduction of several new drug classes and is now well standardized. However, none of the available osteoporosis drugs completely abolishes the occurrence of fractures. Therefore, criteria are needed to determine when the occurrence of a fra...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Confavreux CB,Paccou J,David C,Mehsen N,Leboime A,Thomas T

    更新日期:2010-12-01 00:00:00

  • An unusual presentation of primary hyperparathyroidism: severe hypercalcemia and multiple brown tumors.

    abstract::Primary hyperparathyroidism is common, particularly in postmenopausal women. Since the introduction of routine automated serum calcium assays and the development of assays for intact 1-84 parathyroid hormone, the diagnosis is usually made fortuitously in asymptomatic patients or during evaluation for osteoporosis. As ...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Jouan A,Zabraniecki L,Vincent V,Poix E,Fournié B

    更新日期:2008-03-01 00:00:00

  • New onset of articular inflammatory manifestations in patients with hidradenitis suppurativa under treatment with infliximab.

    abstract:OBJECTIVES:Severe hidradenitis suppurativa (HS), under infliximab, can be associated with different forms of arthritis whose mechanism is unclear. Our objective is to establish the frequency and clinical presentation of new-onset arthritis in HS under infliximab. METHODS:Severe HS patients under infliximab were follow...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Acquacalda E,Roux CH,Albert C,Breuil V,Passeron T,Euller-Ziegler L

    更新日期:2015-10-01 00:00:00

  • Serum levels of IL-1 beta, TNF-alpha, IL-8, and acute phase proteins in seronegative spondyloarthropathies.

    abstract:OBJECTIVES:Some immunological abnormalities have been described in seronegative spondyloarthropathies (SpA). The aim of this study is to determine the serum levels of IL-1beta, TNF-alpha and IL-8, which are proinflammatory cytokines in active and inactive patients with SpA, to compare the results with those of controls...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Sonel B,Tutkak H,Düzgün N

    更新日期:2002-10-01 00:00:00

  • A study on thrombophilic factors in Italian Behcet's patients.

    abstract:BACKGROUND:Behcet's disease (BD) may complicate with arterial and venous thrombosis. The purpose of this work is to evaluate in an Italian group of BD patients with thrombotic events a large panel of inherited and acquired thrombophilic factors. METHODS:Thirty BD patients, of which nine with previously arterial or ven...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Caramaschi P,Poli G,Bonora A,Volpe A,Tinazzi I,Pieropan S,Bambara LM,Biasi D

    更新日期:2010-07-01 00:00:00

  • Detection of basic calcium phosphate crystals in osteoarthritis.

    abstract::Osteoarthritis (OA) is the most common human joint disorder. Its complex pathogenesis remains poorly understood but appears multifactorial. To date, no specific pharmacological agent has been identified to alter the disease course of OA. Calcification of articular cartilage is a hallmark of OA and evidence suggests it...

    journal_title:Joint bone spine

    pub_type: 杂志文章,评审


    authors: MacMullan P,McMahon G,McCarthy G

    更新日期:2011-07-01 00:00:00

  • Acetaminophen as symptomatic treatment of pain from osteoarthritis.

    abstract::Osteoarthritis is a major public health burden. The incidence of osteoarthritis increases with advancing age. Symptomatic treatments aimed at alleviating the pain and thereby restoring joint function form the basis of the treatment. The chronic course requires long-term treatment with special attention to minimizing t...

    journal_title:Joint bone spine

    pub_type: 杂志文章,评审


    authors: Bertin P,Keddad K,Jolivet-Landreau I

    更新日期:2004-07-01 00:00:00

  • Chronic, eventually fatal, Kawasaki-like disease in an adult with spondylarthropathy responding to IVIG therapy.

    abstract::We report on an unusual case of a 40-year-old Caucasian male displaying severe Kawasaki-like symptoms. The disease lasted for seven years before diffuse coronary aneurysms occurred, leading to the patient's death, despite ongoing treatment by intravenous immunoglobulins (IVIGs). The patient had also been suffering fro...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Soltner E,Neel A,Tiab M,Varin S,Cormier G,Maisonneuve H,Maugars Y,Tanguy G,Hamidou M,Berthelot JM

    更新日期:2009-10-01 00:00:00

  • Biological therapy in refractory chronic nonbacterial osteomyelitis: A case series of 19 patients.

    abstract:INTRODUCTION:Chronic non-bacterial osteomyelitis (CNO) represents an autoinflammatory bone disorder. Currently there are no standardized diagnostic or treatment guidelines. The objective of the study is to describe our experience with biological therapy in children with the disease. METHODS:Retrospective chart review ...

    journal_title:Joint bone spine



    authors: Bustamante J,Murias S,Enriquez E,Alcobendas R,Remesal A,De Inocencio J

    更新日期:2020-12-17 00:00:00

  • A 3 mg/kg starting dose of infliximab in active spondyloarthritis resistant to conventional treatments is efficient, safe and lowers costs.

    abstract:OBJECTIVE:We assessed the efficacy, tolerance and cost of a 3 mg/kg starting dose of infliximab for ankylosing spondylitis (AS) and psoriatic arthritis (PsA). METHODS:We retrospectively followed-up 45 biologic-naive consecutive patients (11 with axial AS, 24 with axial and peripheral [mixed] AS and 10 with PsA) who we...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Tenga G,Goëb V,Lequerré T,Bacquet-Deschryver H,Daragon A,Pouplin S,Lanfant-Weybel K,Le Loët X,Dieu B,Vittecoq O

    更新日期:2011-01-01 00:00:00

  • Osteoporotic fractures of the proximal humerus, pelvis, and ankle: epidemiology and diagnosis.

    abstract::Although fractures involving the wrist, spine, and proximal femur are known to be strongly associated with osteoporosis, the underlying bone insufficiency often receives insufficient diagnostic and therapeutic attention. Osteoporosis also increases the risk of fractures at other sites. Low-energy fractures in patients...

    journal_title:Joint bone spine

    pub_type: 杂志文章,评审


    authors: Guggenbuhl P,Meadeb J,Chalès G

    更新日期:2005-10-01 00:00:00

  • Cardiovascular risk in rheumatoid arthritis.

    abstract::The objectives of this review are to discuss data on the cardiovascular risk increase associated with rheumatoid arthritis (RA), the effects of RA treatments on the cardiovascular risk level, and the management of cardiovascular risk factors in patients with RA. Overall, the risk of cardiovascular disease is increased...

    journal_title:Joint bone spine

    pub_type: 杂志文章,评审


    authors: Soubrier M,Barber Chamoux N,Tatar Z,Couderc M,Dubost JJ,Mathieu S

    更新日期:2014-07-01 00:00:00

  • Adolescent idiopathic scoliosis. Is low melatonin a cause?

    abstract:BACKGROUND:Although the cause of adolescent idiopathic scoliosis remains unclear, pinealectomy is followed by the development of scoliosis in chickens. Melatonin is the only hormone secreted by pineal gland. OBJECTIVE:To assess serum melatonin levels in adolescents with idiopathic scoliosis. METHODS:Serum melatonin w...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Sadat-Ali M,al-Habdan I,al-Othman A

    更新日期:2000-01-01 00:00:00

  • A paleoepidemiologic study of diffuse idiopathic skeletal hyperostosis.

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    journal_title:Joint bone spine

    pub_type: 历史文章,杂志文章


    authors: Vidal P

    更新日期:2000-01-01 00:00:00

  • The risk of Sjogren's syndrome in the older adults with gout: A medicare claims study.

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    journal_title:Joint bone spine

    pub_type: 杂志文章,多中心研究


    authors: Singh JA,Cleveland JD

    更新日期:2019-10-01 00:00:00

  • Gout and rheumatoid arthritis, both to keep in mind in cardiovascular risk management: A primary care retrospective cohort study.

    abstract:OBJECTIVES:To assess in one time window cardiovascular risks for both patients with gout and patients with rheumatoid arthritis in a Dutch primary care population. METHODS:Retrospective matched cohort study with data from the electronic health records of 51 Dutch general practices. Participants were patients aged 30 y...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Janssens HJ,Arts PG,Schalk BW,Biermans MC

    更新日期:2017-01-01 00:00:00

  • Current treatments for juvenile idiopathic arthritis.

    abstract::Juvenile idiopathic arthritis (JIA) is a heterogeneous group of diseases, most of which differ from the main adult-onset inflammatory joint diseases. Nonsystemic forms of JIA (oligoarthritis, polyarthritis with or without rheumatoid factors, and spondyloarthropathies) are managed similarly to adult-onset rheumatoid ar...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Quartier P

    更新日期:2010-12-01 00:00:00

  • Time trends in the incidence, prevalence, and severity of rheumatoid arthritis: A systematic literature review.

    abstract:INTRODUCTION:Rheumatoid arthritis (RA) generates a heavy socioeconomic burden. The ability to predict the frequency and severity of socioeconomic effects due to RA is crucial to the development of public health policies. OBJECTIVE:To conduct a systematic literature review of data on the prevalence, incidence, and seve...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Minichiello E,Semerano L,Boissier MC

    更新日期:2016-12-01 00:00:00

  • Streptococcus B septic polyarthritis revealing Good's syndrome.

    abstract::We report on a 46-year-old woman, who developed streptococcus B septic polyarthritis in the context of septicemia of urinary origin. This case revealed a Good's syndrome whose diagnosis was made on the basis of a profound hypogammaglobulinaemia, a large decrease of peripheral B cells and a thymoma disclosed on chest c...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Zabsonré JT,Laoubi K,Kemiche F,Cerf-Payrastre I,Pertuiset E

    更新日期:2012-07-01 00:00:00

  • A possible association between Chlamydiae psittacci infection and temporal arteritis.

    abstract::Some arguments are in favor of the role of Chlamydia in the pathogenesis of atherosclerosis and some vasculitis. Illustrating this possible relation, we report the case of a patient developing consecutively a Chlamydia psittacci infection and a temporal arteritis. A 73-year-old woman, with no significant medical histo...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Bonnet F,Morlat P,Delevaux I,Gavinet AM,Parrens M,Bernard N,Lacoste D,Beylot J

    更新日期:2000-01-01 00:00:00

  • Histone deacetylase inhibitors: new treatment options for inflammatory joint disease?

    abstract::Histone deacetylase inhibitors (HDIs) are a new class of compounds that are being developed for the treatment of malignancies such as cutaneous T-cell lymphoma. HDIs inhibit the removal of acetyl groups from histones. The histone acetylation process is dependent on two enzymes, histone acetyl transferase (HAT) and his...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Toussirot E,Khan KA,Bertolini E,Wendling D,Herbein G

    更新日期:2010-10-01 00:00:00

  • Frohse's arcade syndrome revealing sarcoidosic myopathy.

    abstract::We report the first case of an unusual sarcoidosic muscular involvement, complicated with radial nerve palsy. A 58-year-old woman suffering from a mediastinopulmonary sarcoidosis, was admitted for a driving deficit of the hands with a radially deviation during the wrist extension. She had been given a diagnosis of mot...

    journal_title:Joint bone spine

    pub_type: 杂志文章


    authors: Akasbi N,Tahiri L,Daoudi A,Bendahou M,Harzy T

    更新日期:2011-10-01 00:00:00