Abstract:
:Insoluble, pure protein particles could be advantageous as single-entity vaccines or as carriers for small peptide epitopes. Dense gas anti-solvent precipitation was employed to produce pure protein particles which were found to be insoluble in water. As particulate and multimerized antigens are more immunogenic and hence more advantageous for vaccination, particles were produced via this method using ovalbumin as a model antigen. The particles produced had a mean diameter of approximately 300nm, and remained as discrete particles at low pH. At neutral pH or in the presence of electrolyte, the particles exhibited predictable flocculation behaviour to produce aggregates 1-5microm in diameter. Immunisation of mice with these flocculates elicited specific ovalbumin antibody production, T-cell proliferation and a cytotoxic T-cell response, all in the absence of adjuvant. Thus, dense gas processing could be used as a generic method to produce pure protein particulate vaccines.
journal_name
Vaccinejournal_title
Vaccineauthors
White PJ,Anastasopoulos F,Church JE,Kuo CY,Boyd BJ,Hickey PL,Tu LS,Burns P,Lew AM,Heath WR,Davey GM,Pouton CWdoi
10.1016/j.vaccine.2008.09.087subject
Has Abstractpub_date
2008-12-09 00:00:00pages
6824-31issue
52eissn
0264-410Xissn
1873-2518pii
S0264-410X(08)01361-3journal_volume
26pub_type
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