Generic construction of single component particles that elicit humoural and cellular immune responses without the need for adjuvants.


:Insoluble, pure protein particles could be advantageous as single-entity vaccines or as carriers for small peptide epitopes. Dense gas anti-solvent precipitation was employed to produce pure protein particles which were found to be insoluble in water. As particulate and multimerized antigens are more immunogenic and hence more advantageous for vaccination, particles were produced via this method using ovalbumin as a model antigen. The particles produced had a mean diameter of approximately 300nm, and remained as discrete particles at low pH. At neutral pH or in the presence of electrolyte, the particles exhibited predictable flocculation behaviour to produce aggregates 1-5microm in diameter. Immunisation of mice with these flocculates elicited specific ovalbumin antibody production, T-cell proliferation and a cytotoxic T-cell response, all in the absence of adjuvant. Thus, dense gas processing could be used as a generic method to produce pure protein particulate vaccines.






White PJ,Anastasopoulos F,Church JE,Kuo CY,Boyd BJ,Hickey PL,Tu LS,Burns P,Lew AM,Heath WR,Davey GM,Pouton CW




Has Abstract


2008-12-09 00:00:00
















  • Inclusion of the value of herd immunity in economic evaluations of vaccines. A systematic review of methods used.

    abstract:OBJECTIVE:The objectives of this review were to identify vaccine economic evaluations that include herd immunity and describe the methodological approaches used. METHODS:We used Kim and Goldie's search strategy from a systematic review (1976-2007) of modelling approaches used in vaccine economic evaluations and additi...


    pub_type: 杂志文章,评审


    authors: Nymark LS,Sharma T,Miller A,Enemark U,Griffiths UK

    更新日期:2017-12-14 00:00:00

  • Infants with low vaccine antibody responses have altered innate cytokine response.

    abstract::We recently identified a population of 10% of infants who respond with sub-protective antibody levels to most routine primary pediatric vaccinations due to altered innate and adaptive immune responses. We term these infants as low vaccine responders (LVRs). Here we report new data showing that TLR7/8 agonist - R848 st...


    pub_type: 杂志文章


    authors: Surendran N,Nicolosi T,Pichichero M

    更新日期:2016-11-11 00:00:00

  • From the patient perspective: the economic value of seasonal and H1N1 influenza vaccination.

    abstract::Although studies have suggested that a patient's perceived cost-benefit of a medical intervention could affect his or her utilization of the intervention, the economic value of influenza vaccine from the patient's perspective remains unclear. Therefore, we developed a stochastic decision analytic computer model repres...


    pub_type: 杂志文章


    authors: Lee BY,Bacon KM,Donohue JM,Wiringa AE,Bailey RR,Zimmerman RK

    更新日期:2011-03-03 00:00:00

  • Immunogenicity, safety and reactogenicity of the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) in 2-17-year-old children with asplenia or splenic dysfunction: A phase 3 study.

    abstract:BACKGROUND:Immunization with pneumococcal vaccines is an important prophylactic strategy for children with asplenia or splenic dysfunction, who are at high risk of bacterial infections (including S. pneumoniae). This study aimed to assess immunogenicity and safety of pneumococcal non-typeable Haemophilus influenzae pro...


    pub_type: 杂志文章,多中心研究


    authors: Szenborn L,Osipova IV,Czajka H,Kharit SM,Jackowska T,François N,Habib MA,Borys D

    更新日期:2017-09-25 00:00:00

  • Quantitative assay for the transforming DNA activity in cell culture products for human use.

    abstract::DNA isolates from a number of established cell lines have been tested for their transforming capacity in the NIH3T3 transformation assay. It has been found that this assay can be used to quantify the amount of transforming DNA at a submicrogram to milligram level. This assay system has been applied to validate the red...


    pub_type: 杂志文章


    authors: van Meel FC,Steenbakkers PG,Bos E

    更新日期:1985-12-01 00:00:00

  • Recombinant pseudorabies virus carrying a plasmodium gene: herpesvirus as a new live viral vector for inducing T- and B-cell immunity.

    abstract::In Balb/c mice, the sterile protective immunity induced by immunization with radiation-attenuated Plasmodium yoelii sporozoites is eliminated by in vivo depletion of CD8+ T lymphocytes, suggesting that cytotoxic T lymphocytes (CTL) against malaria antigens expressed on infected hepatocytes are required for mediating t...


    pub_type: 杂志文章


    authors: Sedegah M,Chiang CH,Weiss WR,Mellouk S,Cochran MD,Houghten RA,Beaudoin RL,Smith D,Hoffman SL

    更新日期:1992-01-01 00:00:00

  • Impact of a clinical interventions bundle on uptake of HPV vaccine at an OB/GYN clinic.

    abstract:INTRODUCTION:HPV vaccine uptake is lowest among young adults. Little is known about the most effective way to decrease missed opportunities (MO) and increase uptake of the vaccine in this vulnerable population. OBJECTIVES:To determine the impact of a clinical intervention bundle on the rate of MO and uptake of the vac...


    pub_type: 杂志文章


    authors: Deshmukh U,Oliveira CR,Griggs S,Coleman E,Avni-Singer L,Pathy S,Shapiro ED,Sheth SS

    更新日期:2018-06-14 00:00:00

  • Implementation of good clinical practice guidelines in vaccine trials in developing countries.

    abstract::The practicalities when applying the ICH GCPs (International Conference on Harmonization 1996 Good Clinical Practices [EU, MHLW, FDA. International Conference on Harmonization Guideline for Good Clinical Practice; 1997] in less developed countries (ldcs) are seldom discussed and we found no guidelines as how to "adapt...


    pub_type: 杂志文章


    authors: Acosta CJ,Galindo CM,Ochiai RL,Danovaro-Holliday MC,Laure-Page A,Thiem VD,Jin Y,Khan MI,Sahito SM,Hamza HB,Park JK,Lee H,Bock H,Elyazeed RA,Albert MJ,Ascaso C,Robles TQ,Ali M,Ngai P,Puri MK,Koo YM,Agtini MD,So

    更新日期:2007-04-12 00:00:00

  • Socioeconomic factors play a more important role in childhood vaccination coverage than parental perceptions: a cross-sectional study in Greece.

    abstract::To identify predictive factors of complete and age-appropriate vaccination status in Greece, we conducted a cross-sectional study, using stratified cluster sampling, among children attending the first year of the Greek Grammar school (about 6 years of age) and their parents/guardians. Almost 88% (N=3878) of pupils in ...


    pub_type: 杂志文章


    authors: Danis K,Georgakopoulou T,Stavrou T,Laggas D,Panagiotopoulos T

    更新日期:2010-02-17 00:00:00

  • Factors associated with a successful expansion of influenza vaccination among pregnant women in Nicaragua.

    abstract:BACKGROUND:Pregnant women are at risk of severe influenza disease and are a priority group for influenza vaccination programs. Nicaragua expanded recommendations to include influenza vaccination to all pregnant women in the municipality of Managua in 2013. METHODS:We carried out a survey among 1,807 pregnant women who...


    pub_type: 杂志文章


    authors: Arriola CS,Vasconez N,Thompson M,Mirza S,Moen AC,Bresee J,Talavera I,Ropero AM

    更新日期:2016-02-17 00:00:00

  • Availability of quality vaccines: the industrial point of view.

    abstract::Availability of vaccines may be temporarily affected by sudden peak demand, coming on top of the vast increases of regular demand that the world has witnessed; this must be addressed by sound planning, and flexibility both from manufacturers and users. Temporary shortages are not structural in nature, and capacity is ...


    pub_type: 杂志文章


    authors: Vandersmissen W

    更新日期:1992-01-01 00:00:00

  • Economic assessment of a high-dose versus a standard-dose influenza vaccine in the US Veteran population: Estimating the impact on hospitalization cost for cardio-respiratory disease.

    abstract:OBJECTIVE:To compare the economic impact of high-dose trivalent (HD) versus standard-dose trivalent (SD) influenza vaccination on direct medical costs for cardio-respiratory hospitalizations in adults aged 65 years or older enrolled in the United States (US) Veteran's Health Administration (VHA). METHODS:Leveraging a ...


    pub_type: 杂志文章


    authors: van Aalst R,Russo EM,Neupane N,Mahmud SM,Mor V,Wilschut J,Chit A,Postma M,Young-Xu Y

    更新日期:2019-07-26 00:00:00

  • Aerosol vaccination induces robust protective immunity to homologous and heterologous influenza infection in mice.

    abstract::Live-attenuated influenza vaccine (LAIV) delivered by large droplet intranasal spray is efficacious against infection. However, many of the large droplets are trapped in the external nares and do not reach the target nasal airway tissues. Smaller droplets might provide better distribution yielding similar protection w...


    pub_type: 杂志文章


    authors: Smith JH,Brooks P,Johnson S,Tompkins SM,Custer KM,Haas DL,Mair R,Papania M,Tripp RA

    更新日期:2011-03-21 00:00:00

  • Effects of imperfect test sensitivity and specificity on observational studies of influenza vaccine effectiveness.

    abstract:BACKGROUND:The recently developed test-negative design is now standard for observational studies of influenza vaccine effectiveness (VE). It is unclear how influenza test misclassification biases test-negative VE estimates relative to VE estimates from traditional cohort or case-control studies. METHODS:We simulated p...


    pub_type: 杂志文章


    authors: Jackson ML,Rothman KJ

    更新日期:2015-03-10 00:00:00

  • Potential of Mw as a prophylactic vaccine against pulmonary tuberculosis.

    abstract::Mycobacterium w (Mw), is a cultivable, non-pathogenic mycobacterium and has been tried extensively as an immunomodulator in leprosy. This has been found to be safe and has shown beneficial immunoprophylactic effect in population based, double blind placebo controlled trials in North India. These effects were also obse...


    pub_type: 杂志文章,随机对照试验


    authors: Katoch K,Singh P,Adhikari T,Benara SK,Singh HB,Chauhan DS,Sharma VD,Lavania M,Sachan AS,Katoch VM

    更新日期:2008-02-26 00:00:00

  • Salivary antibody response to vaccination with meningococcal A/C polysaccharide vaccine in previously vaccinated and unvaccinated Gambian children.

    abstract::Development of salivary antibodies at the age of 4 or 5 years to group A and C meningococcal polysaccharides (MenA/C PS) was studied among Gambian children, who had received MenA/C conjugate or PS vaccine in infancy. There was also a control group of 64 age matched children. IgG, IgA, and secretory Ig concentrations w...


    pub_type: 杂志文章


    authors: Nurkka A,MacLennan J,Jäntti V,Obaro S,Greenwood B,Käyhty H

    更新日期:2000-10-15 00:00:00

  • The adjuvant CLDC increases protection of a herpes simplex type 2 glycoprotein D vaccine in guinea pigs.

    abstract::Herpes simplex virus (HSV) infections are common but there is no vaccine available. We evaluated cationic liposome-DNA complexes (CLDC) as an adjuvant for an HSV gD2 vaccine and compared it to an MPL/Alum adjuvant in a guinea pig model of genital herpes. The addition of CLDC to the gD2 vaccine significantly decreased ...


    pub_type: 杂志文章


    authors: Bernstein DI,Farley N,Bravo FJ,Earwood J,McNeal M,Fairman J,Cardin R

    更新日期:2010-05-07 00:00:00

  • A phase III, randomized controlled study to assess the safety and immunogenicity of a semi-synthetic diphtheria, tetanus and whole-cell pertussis vaccine in Indian infants.

    abstract:BACKGROUND:Reactions to DTwP vaccine are well known and are a matter of great concern, much for the development of next generation combination vaccines. To avoid such reactions which occur from foreign compounds, WHO suggested manufacture of DTwP vaccine using semi-synthetic medium. The phase III trial reported here wa...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Sharma H,Patil V,Sharma D,Kapre S,Jadhav S,Ravetkar S,Kumar R,Bahl S,Parekh S,Chakravarty A

    更新日期:2012-09-21 00:00:00

  • A national examination of pharmacy-based immunization statutes and their association with influenza vaccinations and preventive health.

    abstract:BACKGROUND:A series of state-level statute changes have allowed pharmacists to provide influenza vaccinations in community pharmacies. The study aim was to estimate the effects of pharmacy-based immunization statutes changes on per capita influenza vaccine prescriptions, adult vaccination rates, and the utilization of ...


    pub_type: 杂志文章


    authors: McConeghy KW,Wing C

    更新日期:2016-06-24 00:00:00

  • The use of vaccination to combat multiple introductions of Notifiable Avian Influenza viruses of the H5 and H7 subtypes between 2000 and 2006 in Italy.

    abstract::Since 1999, Italy has been challenged by several epidemics of Notifiable Avian Influenza (NAI) of the H5 and H7 subtypes, occurring in the densely populated poultry areas of northern part of the country. Vaccination with a conventional vaccine containing a seed strain with a different neuraminidase subtype to the fiel...


    pub_type: 杂志文章,评审


    authors: Capua I,Marangon S

    更新日期:2007-06-28 00:00:00

  • Rationale and design of a community-based double-blind randomized clinical trial of an HPV 16 and 18 vaccine in Guanacaste, Costa Rica.

    abstract::We report the rationale, design, methods and details of participation of a community-based, double-blind, randomized clinical trial of an HPV 16 and 18 vaccine conducted in two provinces of Costa Rica to investigate the efficacy and population impact of the vaccine in the prevention of cervical cancer precursors. More...


    pub_type: 杂志文章,随机对照试验


    authors: Herrero R,Hildesheim A,Rodríguez AC,Wacholder S,Bratti C,Solomon D,González P,Porras C,Jiménez S,Guillen D,Morales J,Alfaro M,Cyr J,Morrisey K,Estrada Y,Cortés B,Morera LA,Freer E,Schussler J,Schiller J,Lowy D,S

    更新日期:2008-09-02 00:00:00

  • Characterization of a novel live attenuated infectious bronchitis virus vaccine candidate derived from a Korean nephropathogenic strain.

    abstract::A nephropathogenic K2/01 strain of infectious bronchitis virus (IBV) was attenuated by 170 serial passages in embryonated chicken eggs for possible use as a future IBV vaccine strain. High-growth properties and narrow tissue tropisms (limited replication in respiratory tracts) were achieved by the adaptation process. ...


    pub_type: 杂志文章


    authors: Lee HJ,Youn HN,Kwon JS,Lee YJ,Kim JH,Lee JB,Park SY,Choi IS,Song CS

    更新日期:2010-04-01 00:00:00

  • Synthetic peptides induce antibody against a host-protective antigen of Echinococcus granulosus.

    abstract::The immunogenicity of four synthetic peptides was investigated in sheep. The sequences of the peptides (6, 12/13, 21/22 and 24) were derived from linear, antibody-binding epitopes of the EG95 recombinant protein, a host-protective antigen of the parasite Echinococcus granulosus. Sheep were immunised with either free p...


    pub_type: 杂志文章


    authors: Woollard DJ,Gauci CG,Lightowlers MW

    更新日期:1999-12-10 00:00:00

  • Immunity to avian pneumovirus infection in turkeys following in ovo vaccination with an attenuated vaccine.

    abstract::Fertile turkey eggs after 24 days of incubation were vaccinated in ovo with a commercial live attenuated subtype A avian pneumovirus (APV) vaccine. Hatchability was not adversely affected. When a high dose (10 times maximum commercial dose) of vaccine was tested in maternal antibody negative (MA-) eggs, mild clinical ...


    pub_type: 杂志文章


    authors: Worthington KJ,Sargent BA,Davelaar FG,Jones RC

    更新日期:2003-03-28 00:00:00

  • Generation of recombinant bacillus Calmette-Guérin and Mycobacterium smegmatis expressing BfpA and intimin as vaccine vectors against enteropathogenic Escherichia coli.

    abstract::Enteropathogenic Escherichia coli (EPEC) is an important cause of diarrhea in children. EPEC adheres to the intestinal epithelium and causes attaching and effacing (A/E) lesions. Recombinant Mycobacterium smegmatis (Smeg) and Mycobacterium bovis BCG strains were constructed to express either BfpA or intimin. The entir...


    pub_type: 杂志文章


    authors: Vasconcellos HL,Scaramuzzi K,Nascimento IP,Da Costa Ferreira JM Jr,Abe CM,Piazza RM,Kipnis A,Dias da Silva W

    更新日期:2012-09-07 00:00:00

  • Measurement of Clostridium perfringens beta-toxin production by surface plasmon resonance immunoassay.

    abstract::A rapid and sensitive assay using surface plasmon resonance (SPR) immunoassay has been developed for the detection of Clostridium perfringens beta-toxin. The SPR immunoassay was conducted off-line by passing fermentation broth by a sensor chip coated with a monoclonal antibody specific for C. perfringens beta-toxin. Q...


    pub_type: 杂志文章


    authors: Hsieh HV,Stewart B,Hauer P,Haaland P,Campbell R

    更新日期:1998-05-01 00:00:00

  • Interchangeability and tolerability of two inactivated hepatitis A vaccines in Chinese children.

    abstract::In China, no data are available to evaluate the interchangeability between Chinese domestic inactivated hepatitis A vaccines (Healive) and imported inactivated hepatitis A vaccines (Havrix). A double-blind, randomized controlled study was to compare interchangeability and safety of Healive and Havrix among Chinese chi...


    pub_type: 杂志文章,随机对照试验


    authors: Zhang ZL,Zhu XJ,Wang X,Liang M,Sun J,Liu Y,Gao ZG,Wu JY,Dong XJ,Liu RK,Chen JT,Zhang YQ,Wang W,Zhang LP,Yin W

    更新日期:2012-06-08 00:00:00

  • Efficacy of pneumococcal polysaccharide vaccine in immunocompetent adults: a meta-analysis of randomized trials.

    abstract::The use of pneumococcal polysaccharide vaccine (PPV) is low in some countries, maybe because of doubts regarding its efficacy. This meta-analysis aims at combining evidence from randomized trials of PPV assessing its efficacy in preventing Streptococcus pneumoniae related diseases in immunocompetent adults. In the fou...


    pub_type: 杂志文章,meta分析


    authors: Cornu C,Yzèbe D,Léophonte P,Gaillat J,Boissel JP,Cucherat M

    更新日期:2001-09-14 00:00:00

  • Detecting AIDS restriction genes: from candidate genes to genome-wide association discovery.

    abstract::The screening of common genetic polymorphisms among candidate genes for AIDS pathology in HIV exposed cohort populations has led to the description of 20 AIDS restriction genes (ARGs), variants that affect susceptibility to HIV infection or to AIDS progression. The combination of high-throughput genotyping platforms a...


    pub_type: 杂志文章


    authors: Hutcheson HB,Lautenberger JA,Nelson GW,Pontius JU,Kessing BD,Winkler CA,Smith MW,Johnson R,Stephens R,Phair J,Goedert JJ,Donfield S,O'Brien SJ

    更新日期:2008-06-06 00:00:00

  • Pools of lipidated HTL-CTL constructs prime for multiple HBV and HCV CTL epitope responses.

    abstract::Various peptide-based approaches to simultaneous induction of multiple cytotoxic T lymphocyte (CTL) responses were evaluated as part of ongoing efforts to develop immunotherapeutic vaccines for use in humans. To this end, HLA (human histocompatibility leukocyte antigen)-A2-restricted epitopes from several specific vir...


    pub_type: 杂志文章


    authors: Oseroff C,Sette A,Wentworth P,Celis E,Maewal A,Dahlberg C,Fikes J,Kubo RT,Chesnut RW,Grey HM,Alexander J

    更新日期:1998-05-01 00:00:00