Abstract:
:Different genetic biomarkers have been used to evaluate the pollution effects of mutagenic agents such as metals and also a great variety of chemicals delivered on the environment by human activities. This way, the aim of the present report was to evaluate the effects of inorganic lead in fishes through the frequency of piscine micronuclei and nuclear morphological alterations in peripheral cells, chromosomal aberration frequency and comet assays in blood and kidney cells. Specimens of Hoplias malabaricus received different doses of lead by intra-peritoneal injections at time of 96 h. There was not a significant difference between control and treated groups for the piscine micronucleus and chromosomal aberration assays. In the comet assays there was a significant difference between control and contaminated groups. However, a significant difference between the applied doses was not observed. The results obtained with the comet assays also show that blood presented a higher sensibility than the kidney tissue, possibly due to the acute contamination. Although the results showed the genotoxic potential of lead at the 21 and 63 microg Pb(2 + )/g doses for both tissues, the lowest dose is considered more appropriate for future bioassays.
journal_name
Environ Monit Assessjournal_title
Environmental monitoring and assessmentauthors
Ramsdorf WA,Ferraro MV,Oliveira-Ribeiro CA,Costa JR,Cestari MMdoi
10.1007/s10661-008-0566-1subject
Has Abstractpub_date
2009-11-01 00:00:00pages
77-85issue
1-4eissn
0167-6369issn
1573-2959journal_volume
158pub_type
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