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Epi-laser in situ keratomileusis: comparative evaluation of epithelial separation with 3 microkeratomes.

Abstract：

PURPOSE:To evaluate the cleavage plane, corneal cytoarchitecture, and cell vitality of separated corneal epithelial sheets created with 3 commonly used microkeratomes. SETTING:Laboratories of the Regensburg University Medical Center, Regensburg, Germany. METHODS:Mechanical separation of the epithelial layer in 10 porcine eyes and 2 human eyes was performed with 3 different microkeratomes (Amadeus II, Zyoptix XP, Epivision). Five of 10 porcine corneas and the 2 human corneas treated with each microkeratome were processed for histology, electron microscopy, and immunohistochemistry. In 5 of 10 porcine corneas, the corneal epithelial sheets were separated from the globe and cell vitality was assessed with the trypan blue dye vitality test. RESULTS:A reproducible epithelial separation with a smooth surface was achieved in all eyes. The cleavage plane was located between the lamina lucida and the lamina densa. Damage to epithelial cells was mainly limited to the cut margins. CONCLUSIONS:Mechanical separation of the epithelial sheet in epithelial laser in situ keratomileusis (epi-LASIK) was safe and reproducible with all evaluated microkeratomes. Immunohistochemistry and electron microscopy showed the cleavage plane in epi-LASIK was between the basal epithelium and the basement membrane at the level of the lamina lucida.

journal_name

J Cataract Refract Surg

journal_title

Journal of cataract and refractive surgery

authors

Herrmann WA,Hillenkamp J,Hufendiek K,Prahs P,Lohmann CP,Helbig H,Kobuch K

doi

10.1016/j.jcrs.2008.06.028

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

1761-6

issue

10

eissn

0886-3350

issn

1873-4502

pii

S0886-3350(08)00699-8

journal_volume

34

pub_type

杂志文章

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