Tetracycline-inducible gene expression in conditionally immortalized mouse podocytes.

Abstract:

BACKGROUND:Conditionally immortalized podocytes are valuable research tools but are difficult to efficiently transfect and do not provide graded transgene expression. METHODS:Conditionally immortalized mouse podocyte cell lines were established employing a tetracycline-inducible system. Glomerular cells, isolated from transgenic mice bear- ing two transgenes, NPHS2-reverse tetracycline-controlled transactivator, rtTA (A transgene) and H2-Kb-thermosensitive SV40 T, ts58A (I transgene), were cloned. One clone (AI podocytes) expressing WT1 and synaptopodin was transfected with pBI-EGFP (enhanced green fluorescent protein, G transgene) and separately with ptTS-Neo (transcriptional suppressor, T transgene) to produce stable transformants, AIG podocytes and AIT podocytes. RESULTS:AIG podocytes expressed EGFP at 33 and 37 degrees C after doxycycline treatment, and retained podocin and rtTA mRNA expression and temperature-sensitive growth regulation. AIT podocytes, transiently transfected with luciferase-BI-EGFP (LG transgene), showed reduced background expression of EGFP and luciferase in the absence of doxycycline. In AITLG podocytes, generated by stable transfection of AIT podocytes with the LG transgene, luciferase expression was tightly regulated by doxycycline in a time- and concentration-dependent manner both at 33 and 37 degrees C, although background expression was not entirely eliminated. These podocytes retained temperature-sensitive growth regulation and expression of podocyte differentiation markers. CONCLUSION:Mouse podocytes expressed tetracycline-induced transgenes efficiently while retaining differentiation markers.

journal_name

Am J Nephrol

authors

Kajiyama H,Titus S,Austin CP,Chiotos K,Matsumoto T,Sakairi T,Kopp JB

doi

10.1159/000151770

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

153-63

issue

3

eissn

0250-8095

issn

1421-9670

pii

000151770

journal_volume

29

pub_type

杂志文章
  • Healthy behaviors, risk factor control and awareness of chronic kidney disease.

    abstract:BACKGROUND/AIMS:The association between chronic kidney disease (CKD) awareness and healthy behaviors is unknown. We examined whether CKD self-recognition is associated with healthy behaviors and achieving risk-reduction targets known to decrease risk of cardiovascular morbidity and CKD progression. METHODS:CKD awarene...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000346712

    authors: Tuot DS,Plantinga LC,Judd SE,Muntner P,Hsu CY,Warnock DG,Gutiérrez OM,Safford M,Powe NR,McClellan WM,REGARDS Investigators.

    更新日期:2013-01-01 00:00:00

  • Cultural links between Salerno and Montecassino.

    abstract::A meeting on the History of Nephrology is an opportunity to discuss the links between science and faith which were split until the 6th century. However, this was changed by Cassiodorus and monasticism. From the times of Abbot Bertario (died 883 AD) in Montecassino, medicine was regarded as an art and a science. Codex ...

    journal_title:American journal of nephrology

    pub_type: 历史文章,杂志文章

    doi:10.1159/000168769

    authors: D'Onorio B

    更新日期:1994-01-01 00:00:00

  • The selective vitamin D receptor activator for albuminuria lowering (VITAL) study: study design and baseline characteristics.

    abstract:BACKGROUND:Patients with diabetic nephropathy are at high risk for further progressive renal function loss. Treatments that decrease albuminuria have been linked with renal and cardiovascular protection. However, even when taking optimal treatment, residual renal and cardiovascular risk remains high which correlates wi...

    journal_title:American journal of nephrology

    pub_type: 杂志文章,随机对照试验

    doi:10.1159/000225903

    authors: Lambers Heerspink HJ,Agarwal R,Coyne DW,Parving HH,Ritz E,Remuzzi G,Audhya P,Amdahl MJ,Andress DL,de Zeeuw D

    更新日期:2009-01-01 00:00:00

  • Use of Renin-Angiotensin System Blockers Increases Serum Potassium in Anuric Hemodialysis Patients.

    abstract:BACKGROUND:Angiotensin converting enzyme inhibitors (ACEi) and angiotensin receptor blockers (ARB) are increasingly used in uremic patients (pts). However, their effect on serum potassium (sK) concentrations in anuric pts on chronic hemodialysis treatment (HD) is controversial. The aim of the study was to evaluate sK b...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000491552

    authors: Movilli E,Camerini C,Gaggia P,Zubani R,Cancarini G

    更新日期:2018-01-01 00:00:00

  • Renal allograft rupture: a report of three cases and review of the literature.

    abstract::This report reviews the features of 149 cases of renal allograft rupture, while adding a detailed description of 3 more cases to the English literature. From November, 1973 to November, 1979, 3 cases of renal allograft rupture occurred in 100 renal transplants performed at Research Medical Center, Kansas City, Mo., an...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000166577

    authors: Thukral R,Mir AR,Jacobson MP

    更新日期:1982-01-01 00:00:00

  • Atorvastatin increases erythropoietin-stimulating agent hyporesponsiveness in maintenance hemodialysis patients: role of anti-inflammation effects.

    abstract::Erythropoietin-stimulating agent (ESA) hyporesponsiveness is aggravated by chronic inflammation in maintenance hemodialysis (MHD) patients. Dyslipidemia is prevalent in MHD patients. Statin therapy has been demonstrated to not only be effective in lowering lipid levels, but also numerous pleiotropic effects including ...

    journal_title:American journal of nephrology

    pub_type: 临床试验,杂志文章

    doi:10.1159/000169658

    authors: Chiang CK,Yang SY,Peng YS,Hsu SP,Pai MF,Huang JW,Hung KY,Wu KD

    更新日期:2009-01-01 00:00:00

  • Changes in the small bowel of symptomatic kidney transplant recipients converted from mycophenolate mofetil to enteric-coated mycophenolate sodium.

    abstract:BACKGROUND/AIMS:Gastrointestinal (GI) symptoms in renal transplant recipients may be caused due to mycophenolic acid (MPA) toxicity. Using small bowel capsule endoscopy (SBCE) we examined the impact of conversion from Mycophenolate Mofetil (MMF) to enteric-coated formulation of Mycophenolate Sodium (EC-MPS) given to tr...

    journal_title:American journal of nephrology

    pub_type: 临床试验,杂志文章

    doi:10.1159/000365360

    authors: Bunnapradist S,Sampaio MS,Wilkinson AH,Pham PT,Huang E,Kuo HT,Anastasi B,Danovitch GM,Lo SK

    更新日期:2014-01-01 00:00:00

  • Creatinine clearance but not serum creatinine alone predicts long-term postoperative survival after lower extremity revascularization.

    abstract:BACKGROUND:Renal insufficiency is a well-described risk factor for perioperative morbidity and shortened survival after major vascular procedures. Due to the potential inaccuracy of serum creatinine levels alone in measuring kidney function, our aim was to determine whether estimated creatinine clearance more consisten...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000098150

    authors: Maithel SK,Pomposelli FB,Williams M,Sheahan MG,Scovell SD,Campbell DR,LoGerfo FW,Hamdan AD

    更新日期:2006-01-01 00:00:00

  • Clinical implications of antineutrophil cytoplasmic antibody test in lupus nephritis.

    abstract::To elucidate the prevalence and clinical implications of antineutrophil cytoplasmic antibody (ANCA) in lupus nephritis (LN), we examined ANCA by indirect immunofluorescence and by ELISA against antilactoferrin (anti-LF) and antimyeloperoxidase (anti-MPO) antibody. To discriminate perinuclear ANCA (pANCA) with antinucl...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000013557

    authors: Chin HJ,Ahn C,Lim CS,Chung HK,Lee JG,Song YW,Lee HS,Han JS,Kim S,Lee JS

    更新日期:2000-01-01 00:00:00

  • Efficacy and safety of glucocorticoids therapy for IgA nephropathy: a meta-analysis of randomized controlled trials.

    abstract:BACKGROUND:Published reports examining the efficacy and safety of glucocorticoids for preserving renal function in immunoglobulin A nephropathy (IgAN) have yielded conflicting results. To systematically evaluate the efficacy and safety of glucocorticoids agents for IgAN, we conducted a meta-analysis of the published ra...

    journal_title:American journal of nephrology

    pub_type: 杂志文章,meta分析,评审

    doi:10.1159/000226129

    authors: Cheng J,Zhang X,Zhang W,He Q,Tao X,Chen J

    更新日期:2009-01-01 00:00:00

  • Membranous nephropathy associated with giant lymph node hyperplasia. A case report with histological and ultrastructural studies.

    abstract::A young woman presented with nephrotic syndrome due to membranous nephropathy and a localized form of giant lymph node hyperplasia (Castleman's disease) occurring as a large intra-abdominal mass. Five months after surgical removal of the mass, only mild proteinuria persisted. Twenty months later a second kidney biopsy...

    journal_title:American journal of nephrology

    pub_type: 杂志文章,评审

    doi:10.1159/000168127

    authors: Ruggieri G,Barsotti P,Coppola G,Spinelli C,Balducci A,Ventola FR,d'Adamo G,Tata MV,Marinozzi V

    更新日期:1990-01-01 00:00:00

  • Protective effect of tonapofylline (BG9928), an adenosine A1 receptor antagonist, against cisplatin-induced acute kidney injury in rats.

    abstract:BACKGROUND/AIMS:Cisplatin (CIS) induces nephrotoxicity partly through renal vasoconstriction and decreased glomerular filtration effects thought to involve adenosine acting on adenosine A(1) receptors (A1Rs). We studied the effect of the orally active, A1R antagonist tonapofylline (BG9928) on biochemical measures of re...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000248762

    authors: Gill A,Wortham K,Costa D,Davis W,Ticho B,Whalley E

    更新日期:2009-01-01 00:00:00

  • Genetic risk factors of amyloidogenesis in familial Mediterranean fever.

    abstract:BACKGROUND/AIMS:Evaluation of the risk factors, and phenotype-genotype correlation of familial Mediterranean fever (FMF) gene (MEFV) and serum amyloid A1 (SAA1) gene polymorphisms in renal amyloidosis. METHODS:We investigated MEFV and SAA1 genotypes (alpha, beta, and gamma isoforms) in 50 FMF patients and 50 healthy c...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000087824

    authors: Delibaş A,Oner A,Balci B,Demircin G,Bulbul M,Bek K,Erdoğan O,Baysun S,Yilmaz E

    更新日期:2005-09-01 00:00:00

  • Role of hypertension in coronary artery disease.

    abstract::Historically, stroke, heart failure and renal insufficiency were seen as the major complications of untreated hypertension. More recently, it has become evident that coronary heart disease is probably the most common outcome of this condition. Unlike the other complications of hypertension, coronary events have not be...

    journal_title:American journal of nephrology

    pub_type: 杂志文章,评审

    doi:10.1159/000169000

    authors: Weber MA

    更新日期:1996-01-01 00:00:00

  • Rhabdomyolysis among Critically Ill Combat Casualties: Long-Term Outcomes.

    abstract:BACKGROUND:Although rhabdomyolysis has been associated with acute kidney injury and mortality in the short term, the long-term consequences of an episode of rhabdomyolysis remain unknown. We sought to identify the long-term outcomes of rhabdomyolysis, including mortality, renal function, and incidence of hypertension (...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000494337

    authors: Faulk T,Walker LE,Howard JT,Janak JC,Sosnov JA,Stewart IJ

    更新日期:2018-01-01 00:00:00

  • Research Themes and Trends in Ten Top-Ranked Nephrology Journals: A Text Mining Analysis.

    abstract:BACKGROUND:Nephrology research is expanding, and harnessing the much-needed information and data for the practice of evidence-based medicine is becoming more challenging. In this study, we used the natural language processing and text mining approach to mitigate some of these challenges. METHODS:We analyzed 17,412 abs...

    journal_title:American journal of nephrology

    pub_type: 杂志文章,评审

    doi:10.1159/000504871

    authors: Zengul FD,Lee T,Delen D,Almehmi A,Ivankova NV,Mehta T,Topuz K

    更新日期:2020-01-01 00:00:00

  • Putative role of endothelial lipase in dialysis patients with hypoalbuminemia and inflammation.

    abstract:BACKGROUND/AIMS:A recent study suggests that inflammation and malnutrition are strongly associated with cardiovascular disease in dialysis patients. Endothelial lipase (EL) is a newly cloned physiological regulator of high-density lipoprotein cholesterol (HDL), which is associated with the progression of atherosclerosi...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000144025

    authors: Fujii H,Fukuda A,Tanaka M,Kojima Y,Ishida T,Hirata K,Fukagawa M

    更新日期:2008-01-01 00:00:00

  • Acquired renal cystic disease in end-stage renal disease: an autopsy study of 155 cases.

    abstract::Autopsy reports on 155 successive end-stage renal disease (ESRD) patients and 147 control patients without ESRD, matched for age, race and gender, were collected. Cystic transformation of the kidneys was noted in 58% of the ESRD patients and 13.6% of the control patients. There were 25 ESRD patients with renal adenoma...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000167988

    authors: Miller LR,Soffer O,Nassar VH,Kutner MH

    更新日期:1989-01-01 00:00:00

  • Fibrinolysis defect in long-term hemodialysis patients with type 2 diabetes mellitus and its relation to metabolic disorders.

    abstract:BACKGROUND/AIMS:Patients with chronic renal failure (CRF) secondary to diabetes mellitus show a high incidence of atherosclerosis with its thrombotic complications. Both CRF and type 2 diabetes mellitus (DM2) results in fibrinolysis defects causally related to atherogenesis and thrombogenesis. It is not well known whet...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000065270

    authors: Opatrný K Jr,Zemanová P,Mares J,Vít L,Opatrná S,Sefrna F,Hejda V,Tomsů M,Eiselt J,Massry SG

    更新日期:2002-09-01 00:00:00

  • Association of co-occurring serious mental illness with emergency hospitalization in people with chronic kidney disease.

    abstract:BACKGROUND/AIMS:Chronic kidney disease (CKD) and serious mental illness (SMI) are both associated with an increased risk for repeated hospitalization. The objective of this study was to determine if co-occurring SMI exacerbates the risk for subsequent hospitalization, particularly through the emergency department (ED),...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000360095

    authors: McPherson S,Barbosa-Leiker C,Daratha K,Short R,McDonell MG,Alicic R,Roll J,Tuttle K

    更新日期:2014-01-01 00:00:00

  • Role of the calices in urine flow with characterization of a caliceopelvic sphincter and reflex.

    abstract:AIM:To study the role of the caliceal system in urine flow. METHODS:Eight patients (6 men, 2 women; mean age 42.6 +/- 7.6 years) with an upper polar renal mass (6 renal cysts; 2 hypernephromas) which did not encroach on the pelvicaliceal system were studied. After mass removal, a manometric catheter was introduced int...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000013391

    authors: Shafik A

    更新日期:1998-01-01 00:00:00

  • Soluble interleukin-2 receptors and beta 2-microglobulin in patients with primary glomerulonephritis.

    abstract::Serum levels of soluble interleukin-2 receptors (IL2R) and of beta 2-microglobulin (beta 2M) were studied with the immunoenzymatic technique in 38 patients with primary glomerulonephritis (GN), in 10 patients with essential hypertension (EH) and in 30 healthy subjects. IL2R correlated with beta 2M (p < 0.05). IL2R and...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000168627

    authors: Rollino C,Roccatello D,Amprimo MC,Cavalli G,Aimo G,Beltrame G,Basolo B,Martina G,Piccoli G

    更新日期:1993-01-01 00:00:00

  • Impact of long-term cinacalcet, ibandronate or teriparatide therapy on bone mineral density of hemodialysis patients: a pilot study.

    abstract:BACKGROUND:Insufficient evidenced-based information is available for the treatment of osteoporosis in hemodialysis (HD) patients. METHODS:In 102 HD patients, bone mineral density (BMD) was measured twice 16 ± 3 months apart. In the second BMD measurement 66 of them had a femoral neck (FN) T-score <-2.5. Of these 66 pa...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000341864

    authors: Mitsopoulos E,Ginikopoulou E,Economidou D,Zanos S,Pateinakis P,Minasidis E,Memmos D,Thodis E,Vargemezis V,Tsakiris D

    更新日期:2012-01-01 00:00:00

  • Novel methodology to evaluate renal cysts in polycystic kidney disease.

    abstract:AIM:To develop and assess a semiautomated method for segmenting and counting individual renal cysts from mid-slice MR images in patients with autosomal dominant polycystic kidney disease (ADPKD). METHODS:A semiautomated method was developed to segment and count individual renal cysts from mid-slice MR images in 241 su...

    journal_title:American journal of nephrology

    pub_type: 临床试验,杂志文章

    doi:10.1159/000358604

    authors: Bae KT,Sun H,Lee JG,Bae K,Wang J,Tao C,Chapman AB,Torres VE,Grantham JJ,Mrug M,Bennett WM,Flessner MF,Landsittel DP,Consortium for Radiologic Imaging Studies of Polycystic Kidney Disease.

    更新日期:2014-01-01 00:00:00

  • Thymoglobulin and its use in renal transplantation: a review.

    abstract::Thymoglobulin (Thymoglobulin®; Genzyme, Cambridge, Mass., USA) is a polyclonal antibody which has been used in the field of transplantation over the last four decades. With an initial hesitancy, it is widely used now in the prevention and treatment of rejection following renal transplantation. Thymoglobulin's lack of ...

    journal_title:American journal of nephrology

    pub_type: 杂志文章,评审

    doi:10.1159/000351643

    authors: Thiyagarajan UM,Ponnuswamy A,Bagul A

    更新日期:2013-01-01 00:00:00

  • Berengario da Carpi.

    abstract::Berengario da Carpi was magister of anatomy and surgery at the University of Bologna from 1502 to 1527. Eustachio and Falloppia defined him as 'the restaurator of anatomy'. He was a great surgeon, anatomist and physician of illustrious patients including Lorenzo II dei Medici, Giovanni dalle Bande Nere, Galeazzo Palla...

    journal_title:American journal of nephrology

    pub_type: 传,历史文章,杂志文章

    doi:10.1159/000013452

    authors: De Santo NG,Bisaccia C,De Santo LS,De Santo RM,Di Leo VA,Papalia T,Cirillo M,Touwaide A

    更新日期:1999-01-01 00:00:00

  • Pyruvate in the correction of intracellular acidosis: a metabolic basis as a novel superior buffer.

    abstract::The review focuses on biochemical metabolisms of conventional buffers and emphasizes advantages of sodium pyruvate (Pyr) in the correction of intracellular acidosis. Exogenous lactate (Lac) as an alternative of natural buffer, bicarbonate, consumes intracellular protons on an equimolar basis, regenerating bicarbonate ...

    journal_title:American journal of nephrology

    pub_type: 杂志文章,评审

    doi:10.1159/000084141

    authors: Zhou FQ

    更新日期:2005-01-01 00:00:00

  • Kidney protection against ischemia/reperfusion injury by myofibrillogenesis regulator-1.

    abstract:BACKGROUND/AIMS:Ischemia/reperfusion (I/R) injury is characterized by cytoskeletal reorganization and loss of polarity in proximal tubule epithelial cells. Previously, we showed that myofibrillogenesis regulator (MR)-1 promoted actin organization in cardiomyocytes. MR-1 is also expressed in the kidney. METHODS:In this...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000360141

    authors: Wang X,Tao T,Ding R,Song D,Liu M,Xie Y,Liu X

    更新日期:2014-01-01 00:00:00

  • Distribution of volumes of individual glomeruli in kidneys at autopsy: association with physical and clinical characteristics and with ethnic group.

    abstract::We have demonstrated considerable variability in the volumes of different glomeruli in given individuals (individual glomerular volume: IGV) in a stereologic study of kidneys at forensic autopsy performed to investigate sudden or unexpected death in people without manifest kidney disease. We review some important asso...

    journal_title:American journal of nephrology

    pub_type: 杂志文章,评审

    doi:10.1159/000327044

    authors: Hoy WE,Hughson MD,Diouf B,Zimanyi M,Samuel T,McNamara BJ,Douglas-Denton RN,Holden L,Mott SA,Bertram JF

    更新日期:2011-01-01 00:00:00

  • Improvement in prognosis of patients with acute renal failure over a period of 15 years: an analysis of 710 cases in a dialysis center.

    abstract::In order to evaluate the changes in causes and outcome of acute renal failure (ARF) during the years 1975-1989, 710 patients treated in our dialysis center were analyzed. We compared the etiology, the severity and catabolic state of ARF, the techniques of renal replacement therapy, which were employed and the ages and...

    journal_title:American journal of nephrology

    pub_type: 杂志文章

    doi:10.1159/000168466

    authors: Biesenbach G,Zazgornik J,Kaiser W,Grafinger P,Stuby U,Necek S

    更新日期:1992-01-01 00:00:00