Abstract:
:The characterization of mice models of portal hypertension (PHT) is lacking in the literature. Therefore, the aim of the present study was to make a histological approach during development of PHT in two models of cirrhosis with PHT compared with one model of isolated PHT. The model of isolated PHT was developed by partial portal vein ligation (PPVL). Two portal hypertensive cirrhotic mice models were developed either by common bile duct ligation (CBDL) or administration of carbon tetrachloride (CCl(4)) subcutaneously (twice weekly, 1 ml/kg). These models represent, respectively, a secondary biliary cirrhosis and alcoholic cirrhosis. Mice were killed at several time points to evaluate liver changes by histological and ultrastructural methods. A correlation was made with portal pressure measurements. Histology revealed the absence of fibrosis or cirrhosis in PPVL mice. They developed an isolated portal hypertension. After CBDL induction, the mice developed the characteristics of cirrhosis after 6 weeks, with simultaneous increase in portal pressures. Fifty percent of the mice had ascites at that time point. Sixteen weeks after administration of CCl(4), a micronodular cirrhotic aspect of the liver was seen associated with signs of portal hypertension. This is the first descriptive study of three widely used animal models in mice, allowing the study of pathophysiological changes in cirrhosis and portal hypertension. The PPVL in mice leads to a model of isolated portal hypertension. Secondary biliary cirrhosis developed after 6 weeks of common bile duct ligation in 50% of the mice that developed ascites. Subcutaneous injection of CCl(4) for 16 weeks induces cirrhosis and portal hypertension, without ascites. Moreover, the present study is the first description of a cirrhotic model in mice developed by subcutaneous injections of CCl(4). Well-described mice models will facilitate use of knock-out or transgenic mice and lead to a better understanding of the underlying molecular pathways in the field of portal hypertension and cirrhosis.
journal_name
Int J Exp Patholjournal_title
International journal of experimental pathologyauthors
Geerts AM,Vanheule E,Praet M,Van Vlierberghe H,De Vos M,Colle Idoi
10.1111/j.1365-2613.2008.00597.xsubject
Has Abstractpub_date
2008-08-01 00:00:00pages
251-63issue
4eissn
0959-9673issn
1365-2613pii
IEP597journal_volume
89pub_type
杂志文章abstract::Non-alcoholic fatty liver disease (NAFLD) is the most common chronic condition of the liver in the western world. There is only little evidence about altered sensitivity of steatotic liver to acute toxic injury. The aim of this project was to test whether hepatic steatosis sensitizes rat liver to acute toxic injury in...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/j.1365-2613.2011.00765.x
更新日期:2011-08-01 00:00:00
abstract::Administration of mercuric chloride (HgCl(2)) to Brown Norway rats causes Th2-dominated autoimmunity with raised immunoglobulin E concentrations and gut vasculitis, both of which are T-cell dependent, peak at 14 days after starting HgCl(2) and then spontaneously resolve. If animals are re-challenged with HgCl(2) 6 wee...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/j.0959-9673.2004.00382.x
更新日期:2004-06-01 00:00:00
abstract::The objective of this study was to assess whether ascorbic acid (AA), an intracellular anti-oxidant critical for neuronal protection, when added to artificial cerebrospinal fluid (ACSF), is able to protect hippocampal (HPC) formation slice preparations from ageing. In this research, the micro-electroencephalographic (...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/j.1365-2613.2012.00838.x
更新日期:2012-12-01 00:00:00
abstract::In this study, the extent of angiogenesis, evaluated as microvascular volume density, immunoreactivity of tumour cells to erythropoietin (Epo) and of endothelial cells to Epo receptor (EpoR) have been correlated in human primary melanoma specimens. Results showed that Epo/EpoR expression correlate with angiogenesis an...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/j.1365-2613.2010.00731.x
更新日期:2010-12-01 00:00:00
abstract::Administration of 25 mg/kg/day methyl clofenapate to Alpk/APfSD rats for up to 4 days gave rise to hepatomegaly resulting from a combination of hepatocyte hyperplasia, mainly in the periportal region of the lobule, and centrilobular cell hypertrophy. In hepatocytes undergoing mitosis there was a redistribution of dens...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract::During mild (one to two pairs of worms) and prolonged (23 weeks or more) mouse infections with Schistosoma mansoni, but not with S. japonicum, periovular granulomas and fibrosis were seen to be preferentially located along periportal tissues. This caused fibrotic expansion of the portal spaces on a background of norma...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract::The liver sinusoidal endothelial cell (LSEC) is damaged by many toxins, including oxidants and bacterial toxins. Any effect on LSECs of the Pseudomonas aeruginosa virulence factor, pyocyanin, may be relevant for systemic pseudomonal infections and liver transplantation. In this study, the effects of pyocyanin on in vi...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/j.1365-2613.2008.00602.x
更新日期:2008-12-01 00:00:00
abstract::Tumour growth is angiogenesis dependent. Thrombosis and thromboembolism are very common in cancer patients. These patients are often treated with heparin as an anti-coagulant. Many tumour angiogens, including VEGF165, and endogenous anti-angiogenesis factors bind heparin tightly. Using the non-surgical mesenteric-wind...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1046/j.1365-2613.2000.00150.x
更新日期:2000-06-01 00:00:00
abstract::The field of study concerning promotion and/or inhibition of angiogenesis has gathered much attention in the scientific community. A great deal of work has been invested towards defining reproducible assays to gauge for promotion or inhibition of angiogenesis in response to drug treatments or growth conditions. Two co...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/iep.12043
更新日期:2013-12-01 00:00:00
abstract::To confirm the existence of the cervical thymus and the development of cervical thymoma in thymoma-prone BUF/Mna (BUF) rats, we examined cervical organs and adjacent tissues, and thoracic thymic tissues of the three inbred strains, BUF, ACI/NMna (ACI), and WKY/NCrj (WKY), and 11 congenic strains, in which genetic regi...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/j.1365-2613.2010.00755.x
更新日期:2011-04-01 00:00:00
abstract::Chronic hepatitis B virus (HBV) infection is a major global cause of hepatocellular carcinoma (HCC). Individuals who are chronic carriers have a greater than 100-fold increased relative risk of developing the tumour. Several mechanisms of HBV-induced HCC have been proposed. Integration of HBV DNA into the genome of he...
journal_title:International journal of experimental pathology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2613.2001.iep0082-0077-x
更新日期:2001-04-01 00:00:00
abstract::Cyclophilins are proteins which are remarkably conserved through evolution; moreover they have been found in every possible existing organism, which indicates their fundamental importance. Due to their enzymatic properties, multiplicity, cellular localization and role in protein folding they belong to the group of pro...
journal_title:International journal of experimental pathology
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2613.1999.00128.x
更新日期:1999-12-01 00:00:00
abstract::C-cell tumours of the thyroid gland are among the most common spontaneous neoplasms of the laboratory rat. With the exception of calcitonin, little attention has been paid to the secretory peptides of C cells during the development of neoplasia. Of these peptides, somatostatin (SS) is of particular interest because it...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/j.0959-9673.2004.00367.x
更新日期:2004-02-01 00:00:00
abstract::This study tested the hypothesis that different doses of nandrolone decanoate (ND) will cause changes in the estrous cycle and ovarian tissue of adult rats; and investigated the duration of the recovery period that is sufficient to restore the damage in the animals treated with different doses. Wistar rats were treate...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/iep.12144
更新日期:2015-10-01 00:00:00
abstract::Pterygium is a surface ocular lesion that is associated with chronic UV exposure. The primary effect is a solar actinic elastosis within the stroma. All the other changes are secondary. Pterygium is characterized by proliferation, inflammatory infiltrates, fibrosis, angiogenesis and extracellular matrix breakdown. The...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/j.1365-2613.2009.00665.x
更新日期:2009-12-01 00:00:00
abstract::The roles of inducible nitric oxide synthase (iNOS) in the development and healing of gastric ulcers have not been fully characterized. We characterized iNOS expression in experimentally induced ulcers in rat and mouse stomachs and investigated the roles of iNOS using iNOS gene-deficient (iNOS-/-) mice and wildtype mi...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/j.1365-2613.2003.00357.x
更新日期:2003-10-01 00:00:00
abstract::(Pre)neoplastic lesions in livers of rats induced by diethylnitrosamine are characterized by elevated activity of the first irreversible enzyme of the oxidative branch of the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase (G6PD), for production of NADPH. In the present study, the activity of G6PD, ...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/j.1365-2613.2008.00582.x
更新日期:2008-08-01 00:00:00
abstract::Although the exact mechanism for the progression of myelofibrosis in acute megakaryoblastic leukaemia is unclear, certain humoral factors released from the proliferating megakaryoblasts that are unable to store these factors in their defective alpha-granules, including platelet derived growth factor (PDGF), fibroblast...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::The Meriones unguiculatus (Mongolian) gerbil has demonstrated significant prostatic responses to hormonal treatments, and to drugs against human prostatic hyperplasia. Spontaneous neoplasia develops in the older animals. Thirty gerbils (age 18 months) were divided into non-affected and prostatic lesion bearers and the...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/j.1365-2613.2007.00550.x
更新日期:2008-02-01 00:00:00
abstract::An important means of determining how amyloid-beta peptide (Abeta) affects cells is to identify specific macromolecular targets and assess how Abeta interaction with such targets impacts on cellular functions. On the one hand, cell surface receptors interacting with extracellular Abeta have been identified, and their ...
journal_title:International journal of experimental pathology
pub_type: 杂志文章,评审
doi:10.1111/j.0959-9673.2005.00427.x
更新日期:2005-06-01 00:00:00
abstract::A recent global gene expression profiling study unexpectedly showed that activated oncogenic NRAS may recruit neural precursor cell expressed, developmentally downregulated 4L (Nedd4L; a human homologue of Nedd4-2) in cultured melanoma cells. However, whether Nedd4L was expressed in melanoma tissues or participated in...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/iep.12051
更新日期:2014-02-01 00:00:00
abstract::Ureteropelvic junction (UPJ) obstruction is the most frequently observed cause of obstructive nephropathy in children. Neonatal and foetal animal models have been developed that mimic closely what is observed in human disease. The purpose of this review is to discuss how obstructive nephropathy alters kidney histology...
journal_title:International journal of experimental pathology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2613.2010.00727.x
更新日期:2011-06-01 00:00:00
abstract::Experiments on the relationship between the hepatocellular transport of endogenous or exogenously loaded bile acids (sodium taurocholate, TC, 0.5 mumol/min/100 g body wt) and horseradish peroxidase (HRP) or immunoglobulin A (IgA) (0.5 mg/100 g body wt) were carried out on anaesthetized Wistar rats. The time course of ...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::The use of non-specific inhibitors of tissue non-specific alkaline phosphatase (TNSALP) in pre-adipocytes blocks intracellular lipid accumulation. TNSALP is also expressed in hepatocytes, which are known to accumulate lipid in a similar manner to pre-adipocytes. The purpose of this study was to use specific silencing ...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/iep.12243
更新日期:2017-10-01 00:00:00
abstract::Blister formation and skin damage can be induced by BaP1, a haemorrhagic metalloproteinase from the venom of the snake Bothrops asper. Pathological changes in the skin were investigated after intramuscular injections of Bothrops asper haemorrhagic metalloproteinase BaP1. Blisters developed within the first hour, with ...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::Naturally developing spontaneous osteoarthritis (OA) is seen in a number of small animals one of which is the STR/ORT mouse, an accepted model of human OA. A histological evaluation of the patello-femoral joints of 37 male STR/ORT mice has shown features that are inconsistent with the disease in human joints. These in...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::Systemic hypertension, characterized by elevated blood pressure ≥140/90 mm Hg, is a major modifiable risk factor for cardiovascular disease. Hypertension also associates with non-alcoholic fatty liver disease (NAFLD), which is becoming common due to a modern diet and lifestyle. The aim of the present study was to exam...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/iep.12369
更新日期:2020-10-01 00:00:00
abstract::Nonalcoholic steatohepatitis (NASH) is one of the life-threatening hepatic diseases; however, its pathogenesis is still unknown. To evaluate the causative role of hyperlipidaemia and high-fat diet, we compared C57BL/6 mice with inherited hyperlipidaemic model mice (LDLR(-/-)mice and ApoE(-/-) mice) fed a normal or a h...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/j.0959-9673.2004.00401.x
更新日期:2004-12-01 00:00:00
abstract::Summary K-ras mutations are found in 40-50% of human colorectal adenomas and carcinomas, but their functional contribution remains incompletely understood. Here, we show that a conditional mutant K-ras mouse model (K-ras(Asp12)/Cre), with transient intestinal Cre activation by beta-Naphthoflavone (beta-NF) treatment, ...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/j.1365-2613.2009.00667.x
更新日期:2009-10-01 00:00:00
abstract::Mouse phrenic nerve-hemidiaphragms were stimulated in vitro in the presence of the anticholinesterase ecothiopate iodide and prepared for light and electron microscopy at different times during and after the appearance of prolonged contractions localized at the endplate. The earliest changes were at the subsynapse, wi...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00