Abstract：
:Cdc42 and Rac1 Rho family GTPases, and their interacting protein IQGAP1 are the key regulators of cell polarity. We examined the role of Cdc42 and IQGAP1 in establishing the polarity of mouse oocyte and regulation of meiotic and mitotic divisions. We showed that Cdc42 was localized on the microtubules of meiotic and mitotic spindle and in the cortex of mouse oocytes and cleaving embryos. IQGAP1 was present in the cytoplasm and cortex of growing and fully-grown oocytes. During maturation it disappeared from the cortex and during meiotic and mitotic cytokinesis it concentrated in the contractile ring. Toxin B inhibition of the binding activity of Cdc42 changed the localization of IQGAP1, inhibited emission of the first polar body, and caused disappearance of the cortical actin without affecting the migration of meiotic spindle. This indicates, that in maturing oocytes accumulation of cortical actin is not indispensable for spindle migration. In zygotes treated with toxin B actin cytoskeleton was rearranged and the first and/or subsequent cytokinesis were inhibited. Our results indicate that Cdc42 acts upstream of IQGAP1 and is involved in regulation of cytokinesis in mouse oocytes and cleaving embryos, rather than in establishing the polarity of the oocyte.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Bielak-Zmijewska A,Kolano A,Szczepanska K,Maleszewski M,Borsuk Edoi
10.1016/j.ydbio.2008.06.039subject
Has Abstractpub_date
2008-10-01 00:00:00pages
21-32issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(08)01047-6journal_volume
322pub_type
杂志文章abstract：:Nematodes provide a useful experimental system with which to investigate the evolution of development at the cellular, genetic, and molecular levels. Building on an understanding of vulval development in Caenorhabditis elegans, analysis of vulval development has been extended to a number of other species in three fami...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1006/dbio.1996.0035
更新日期：1996-02-01 00:00:00
abstract：:A group of proteins judged on several criteria to be yolk proteins have been isolated from a homogenate of the nematode Caenorhabditis elegans. Comparison of partial proteolysis fragments indicates that the two bands of a 170,000-dalton doublet (yp170) are closely related; bands observed at 115,000 daltons (yp115) and...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1983-03-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2001-04-15 00:00:00
abstract：:Hair shafts are produced from stem cells located in the bulge. Our knowledge of the genetic pathways regulating cell fate acquisition in the immediate descendents of these stem cells, and fate maintenance in their committed progeny, is still incomplete. One pathway involved in fate maintenance within the hair matrix i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.11.021
更新日期：2009-02-15 00:00:00
abstract：:The fertilized ascidian egg is thought to be comprised of distinct regions of tissue-specific cytoplasmic determinants. This idea was tested by bisecting fertilized eggs into egg fragments and culturing them until the unoperated controls developed into larvae. Fertilized eggs were bisected using a microsurgical method...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1988-09-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1992-11-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2010-10-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90188-7
更新日期：1989-10-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2020-01-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00088-x
更新日期：2003-03-15 00:00:00
abstract：:Recent data suggest that dental cells utilize the evolutonarily conserved Notch-mediated intercellular signaling pathway to regulate their fates. Here we report on the expression and regulation of Delta1, a transmembrane ligand of the Notch receptors, during mouse odontogenesis. Delta1 is weakly expressed in dental ep...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9092
更新日期：1998-12-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.11.007
更新日期：2008-01-15 00:00:00
abstract：:Experiments in which the developing gut of avian embryos was back-transplanted to permit the bowel to interact with the developing neural tube were undertaken. Segments of intestine from 4-day quail embryos were implanted between the somites and neural tubes of chick embryos of 7 to 24 somites. The spinal cord respond...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90486-6
更新日期：1987-12-01 00:00:00
abstract：:In this paper we present a structural and functional characterization of a new sea urchin embryo transcription factor, SpRunt-1. This factor was isolated by means of its specific interaction with a cis-regulatory target site of the CyIIIa gene. Here we show that this target site, the P7I site, is required for normal e...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0050
更新日期：1996-02-25 00:00:00
abstract：:T-box family transcription factors play many roles in Metazoan development. Here we characterise Tbx6r, a unique Tbx6 paralogue isolated from the amphibian Xenopus. The evolution and developmental integration of this divergent T-box gene within the vertebrates reveals an unexpected level of plasticity within this cons...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.01.013
更新日期：2010-04-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2018.11.009
更新日期：2019-04-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0168
更新日期：1996-07-10 00:00:00
abstract：:Eggs of the newt, Cynops pyrrhogaster, arrested at the second meiotic metaphase are activated by sperm at fertilization and then complete meiosis to initiate development. We highly purified a sperm factor for egg activation from a sperm extract with several chromatographies. The purified fraction containing only a 45 ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.04.019
更新日期：2007-06-15 00:00:00
abstract：:Two distinct DNA ligases are expressed during early sea urchin embryogenesis. A light form (50 kDa) is found in unfertilized eggs (oocyte form) and a heavier enzyme (110 kDa) is observed at the two-cell stage (embryonic form). The chronology of the change reveals that the embryonic form is detected 90 min after fertil...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90479-9
更新日期：1987-11-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.01.016
更新日期：2006-04-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0158
更新日期：1996-07-10 00:00:00
abstract：:Autoradiography has been used to confirm and to extend previous microspectrophotometric studies (Doerder and DeBault, 1975) on the timing of DNA synthesis during conjugation in Tetrahymena thermophila. The majority of DNA synthesis occurs at the expected periods preceding gamete formation and the two postzygotic divis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90500-8
更新日期：1987-12-01 00:00:00
abstract：:Male germline stem cells (GSCs) in Drosophila melanogaster divide asymmetrically by orienting the mitotic spindle with respect to the niche, a microenvironment that specifies stem cell identity. The spindle orientation is prepared during interphase through stereotypical positioning of the centrosomes. We recently demo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.10.010
更新日期：2012-01-01 00:00:00
abstract：:In this investigation, we characterize the embryonic and adult actins and describe the embryonic expression of a muscle actin in the ascidian Styela. Two-dimensional polyacrylamide gel electrophoresis showed that embryos, tadpole larvae, and adult organs contain three major and two minor isoforms of actin. Two of the ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90404-0
更新日期：1987-10-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.05.522
更新日期：2008-08-01 00:00:00
abstract：:To examine the relationship between growth hormone (GH) and insulin-like growth factor 1 (IGF1) in controlling postnatal growth, we performed a comparative analysis of dwarfing phenotypes manifested in mouse mutants lacking GH receptor, IGF1, or both. This genetic study has provided conclusive evidence demonstrating t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9975
更新日期：2001-01-01 00:00:00
abstract：:Although a large superfamily of G-protein-coupled receptors serves multiple functions, little is known about their functional activation during ontogeny. To examine the functional activation of the mu-opioid receptor (MOR) and the delta-opioid receptor (DOR) during development, sections of mouse embryos and fetuses fr...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2003-01-01 00:00:00
abstract：:In Xenopus gastrulation, the involuting mesodermal and non-involuting ectodermal cells remain separated from each other, undergoing convergent extension. Here, we show that Eph-ephrin signaling is crucial for the tissue separation and convergence during gastrulation. The loss of EphA4 function results in aberrant gast...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.12.012
更新日期：2011-02-15 00:00:00
abstract：:To identify guanine nucleotide binding proteins (G-proteins) in sea urchin eggs and to investigate their role in signal transduction at fertilization, we used cholera toxin (CTX) and pertussis toxin (PTX), which catalyze the specific ADP-ribosylation of G-proteins. Cell surface complex, consisting of plasma membranes ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90260-0
更新日期：1987-04-01 00:00:00
