Abstract:
:Pemphigus vulgaris (PV) is an autoimmune bullous disease caused by immunoglobulin G (IgG) autoantibodies against desmoglein 3 (Dsg3). We have generated an active disease mouse model for PV by adoptive transfer of Dsg3(-/-) lymphocytes. In this study, we investigated the benefits and limitations of this model as a tool to evaluate various immunosuppressive therapeutic strategies. We used the following three measurements to evaluate the effects of the drugs during the time course: Dsg3 enzyme-linked immunosorbent assay scores that represent the level of production of anti-Dsg3 IgG, body weight loss that reflects the severity of oral erosions and PV score that reflects the extent of skin lesions. We examined various immunosuppressive agents currently used to treat patients with PV model mice in preventive protocol. Cyclophosphamide almost completely suppressed the production of anti-Dsg3 IgG, development of body weight loss and the appearance of the PV phenotype in contrast with the control group without the drug. Azathioprine, cyclosporin A and tacrolimus hydrate also showed suppressive effects to various degrees. However, methylprednisolone and dexamethasone failed to show significant effects in contrast to the findings reported in humans. Knowing the advantages and limitations of this model will provide an important foundation for the future evaluation and development of novel therapeutic strategies.
journal_name
Exp Dermatoljournal_title
Experimental dermatologyauthors
Takae Y,Nishikawa T,Amagai Mdoi
10.1111/j.1600-0625.2008.00776.xsubject
Has Abstractpub_date
2009-03-01 00:00:00pages
252-60issue
3eissn
0906-6705issn
1600-0625pii
EXD776journal_volume
18pub_type
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journal_title:Experimental dermatology
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journal_title:Experimental dermatology
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journal_title:Experimental dermatology
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journal_title:Experimental dermatology
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journal_title:Experimental dermatology
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1034/j.1600-0625.2001.100107.x
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pub_type: 杂志文章,评审
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journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 信件
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1111/j.1600-0625.2009.00868.x
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pub_type: 杂志文章,评审
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journal_title:Experimental dermatology
pub_type: 杂志文章
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更新日期:2015-05-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.1998.tb00316.x
更新日期:1998-08-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.1998.tb00300.x
更新日期:1998-02-01 00:00:00
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pub_type: 信件
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更新日期:2012-12-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章,评审
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更新日期:2014-12-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.0906-6705.2006.00428.x
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