Abstract:
:The selection of housekeeping genes is critical for gene expression studies. To address this issue, four candidate housekeeping genes, including several commonly used ones, were investigated in oral squamous cell carcinoma cell lines. A simple quantitative RT-PCR approach was employed by comparing relative expression of the four candidate genes within two cancerous cell lines (HN6 and HN31) and one noncancerous cell line (HaCaT) treated or not with EGF and TGF-beta1. Data were analyzed using ANOVA followed by the NormFinder software program. On this basis, stability of the candidate housekeeping genes was ranked and non statistical differences were found using ANOVA test. On the other hand, the NormFinder was able to show that GAPD and TUBB presented the less variable results, representing appropriated housekeeping genes for the samples and conditions analyzed. In conclusion, this study suggests that the GAPD and the TUBB represent adequate normalizers for gene profiling studies in OSCC cell lines, covering, respectively, high and low expression levels genes.
journal_name
Oral Oncoljournal_title
Oral oncologyauthors
Campos MS,Rodini CO,Pinto-Júnior DS,Nunes FDdoi
10.1016/j.oraloncology.2008.03.019subject
Has Abstractpub_date
2009-02-01 00:00:00pages
121-6issue
2eissn
1368-8375issn
1879-0593pii
S1368-8375(08)00106-1journal_volume
45pub_type
杂志文章相关文献
ORAL ONCOLOGY文献大全abstract::Traumatic eosinophilic granuloma of the oral mucosa, also known as eosinophilic ulcer, is considered to be a reactive lesion of unknown aetiology. It usually presents as a tongue ulcer and injury has been considered to play a role in its cause. We present a 72-year-old man who had suffered multiple episodes of recurre...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/s1368-8375(97)00014-6
更新日期:1997-09-01 00:00:00
abstract::The aims of this study were to assess a cohort of patients with head and neck squamous cell carcinoma (HNSCC) for: (1) the prevalence of synchronous distant metastases (DM) as detected by the initial staging by using (18)F-FDG PET/CT, (2) the prevalence of metachronous DM, and (3) the validity of published risk factor...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2011.05.011
更新日期:2011-07-01 00:00:00
abstract::Cyclooxygenase-2 (COX-2) is overexpressed in various types of human malignancies, including oral cancers. Recent studies have shown that mast cell-derived protease tryptase can induce COX-2 expression by the cleavage of proteinase-activated receptor-2 (PAR-2). Actinic cheilitis (AC) is a premalignant form of lip cance...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2008.05.019
更新日期:2009-03-01 00:00:00
abstract::Clear cell tumours, in the head and neck region, are usually derived from salivary or odontogenic tissues, or may be metastatic. A few clear cells may be present in odontogenic cysts, while, odontogenic neoplasms composed predominantly of clear cells are quite rare. They include calcifying epithelial odontogenic tumou...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/s1368-8375(01)00038-0
更新日期:2002-02-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) A is known to play an important role in tumor angiogenesis. The additional members of the VEGF family, VEGF B, C and D have been discovered. VEGF C and D show some selectivity toward lymphatic endothelial cells. However, whether VEGF family members play a role in tumor angioge...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/s1368-8375(03)00127-1
更新日期:2004-01-01 00:00:00
abstract::We hope to establish a new readmission prediction score for patients with head and neck cancer (HNC) after major cancer surgery. A retrospective cohort study was conducted from the clinical and cancer registry data at Kaohsiung Veterans General Hospital. We included the data of patients with newly diagnosed HNC who un...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2020.104772
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVES:Perineural invasion (PNI) is a common histopathological finding in head and neck squamous cell carcinoma (HNSCC). We aimed to explore the molecular mechanisms involved in PNI and the role of PNI as an aggressive pathological feature. MATERIALS AND METHODS:We used data from The Cancer Genome Atlas (TCGA) to ...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2018.09.005
更新日期:2018-11-01 00:00:00
abstract::The role of the major risk factors, tobacco smoking and alcohol consumption, on trends in intra-oral cancer mortality in England and Wales between 1911 and 1990 was investigated, using lung cancer and liver cirrhosis as surrogate markers for smoking and drinking. Standardised mortality data on lung cancer and liver ci...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/s1368-8375(00)00020-8
更新日期:2000-07-01 00:00:00
abstract::Oral carcinoma (OSCC) is one of the most important causes of cancer death worldwide. OSCC cell lines and preclinical rodent models are crucial to addressing the mechanisms of OSCC and helping the development of new therapeutic strategies and interventions. The establishment of murine OSCC cell lines and syngeneic mode...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2019.06.026
更新日期:2019-08-01 00:00:00
abstract:OBJECTIVES:To perform a systematic review of 1762 patients to comprehensively assess the benefit of altered-fractionation radiotherapy (ART) in early stage glottic carcinoma (ESGC). MATERIALS AND METHODS:Studies were identified in PubMed and EMBASE. Inclusion criteria were: (1) diagnosis of squamous cell ESGC (Tis, T1...
journal_title:Oral oncology
pub_type: 杂志文章,meta分析
doi:10.1016/j.oraloncology.2019.04.007
更新日期:2019-06-01 00:00:00
abstract::CD40 is expressed on basal keratinocytes and Squamous Cell Cancer of the Head and Neck (SCCHN) tumor cells in vivo and in vitro. CD40 ligation reduces proliferation of SCCHN cell lines and enhances EGFr mediated inhibition of proliferation. We investigated the mechanisms of CD40 function and EGFr cross-communication i...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2004.11.005
更新日期:2005-05-01 00:00:00
abstract:OBJECTIVES:The treatment strategy of early stage oral squamous cell carcinoma's (OSCC) resected with close or involved margins is a returning point of discussion. In this study we reviewed the consequences of re-resection (RR), postoperative radiotherapy (PORT) or watchful waiting (WW). PATIENTS AND METHODS:Two-hundre...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2014.02.014
更新日期:2014-06-01 00:00:00
abstract::Tumor-infiltrating lymphocytes (TILs) are considered to represent immune reactions of the host to a malignant tumor. Programmed death receptor ligand-1 (PD-L1) is a surface protein that blocks the function of T lymphocytes and is expressed on cancer cells. Tumor-associated fibroblasts (TAFs), which influence tumor gro...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2011.08.007
更新日期:2011-12-01 00:00:00
abstract:OBJECTIVES:Several studies have investigated the relationship between the use of dentures and the duration of denture use and cancer development. Of particular interest is whether ill-fitting dentures increase the likelihood of the development of oral cancer. We conducted a meta-analysis to determine the relationship b...
journal_title:Oral oncology
pub_type: 杂志文章,meta分析
doi:10.1016/j.oraloncology.2014.08.002
更新日期:2014-11-01 00:00:00
abstract:OBJECTIVES/HYPOTHESIS:We review the use of topical chemoprevention agents in patients with oral potentially malignant disorders (PMD). METHODS:A systematic review of studies on topical chemoprevention agents for oral PMD from 1946 to November 2016 was conducted using the MEDLINE database, Embase, and Cochrane Library....
journal_title:Oral oncology
pub_type: 杂志文章,评审
doi:10.1016/j.oraloncology.2017.02.014
更新日期:2017-04-01 00:00:00
abstract:OBJECTIVES:Results of the Dutch multi-institutional trial on sentinel lymph node (SLN) biopsy in oral cancer. PATIENTS AND METHODS:Patients were consecutively enrolled from 4 institutions, with T1/T2 oral cancer and cN0 neck based on palpation and ultrasound guided fine needle aspiration cytology. Lymphatic mapping co...
journal_title:Oral oncology
pub_type: 杂志文章,多中心研究
doi:10.1016/j.oraloncology.2014.07.020
更新日期:2014-10-01 00:00:00
abstract:OBJECTIVES:Log odds of positive lymph nodes (LODDS) was reported to be significantly associated with prognosis in several malignant tumors. However, few are the studies on the correlation between LODDS and overall survival (OS) in patients with oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS:A retrospective...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2020.104709
更新日期:2020-09-01 00:00:00
abstract::Stage of disease at the diagnosis of oral cancer is thought to be a significant factor in prognosis and outcome (International Agency for Research on Cancer/World Health Organization, 2014). Unfortunately, we continue to diagnose almost 2/3 of these cancers at advanced stages of disease despite the ongoing research fo...
journal_title:Oral oncology
pub_type: 杂志文章,评审
doi:10.1016/j.oraloncology.2014.09.005
更新日期:2014-12-01 00:00:00
abstract::In recent years, studies on the malignant potential of oral lichen planus (OLP) provided clinical evidence that patients affected by OLP have an increased risk to develop oral squamous cell carcinoma (OSCC); nevertheless, controversies still exist as to whether OLP has inherent predisposition to become malignant, or n...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/s1368-8375(00)00096-8
更新日期:2001-04-01 00:00:00
abstract::We would like to compare the effectiveness of image-guided (IGRT) and intensity-modulated (IMRT) radiotherapy to spare the larynx in head and neck cancer patients. A retrospective review of 48 patients undergoing radiation for non-laryngeal and non-hypopharyngeal head and neck cancers. Mean laryngeal and hypopharyngea...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2010.01.010
更新日期:2010-04-01 00:00:00
abstract:OBJECTIVES:Though the effect of age has been studied in some cancer types, its prognostic significance in oropharyngeal squamous cell carcinoma (OPSCC) remains controversial. Our purpose is to determine the impact of age at diagnosis on overall survival (OS) and disease-specific survival (DSS) in patients with OPSCC. I...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2013.12.013
更新日期:2014-05-01 00:00:00
abstract:OBJECTIVES:A multi-disciplinary team (MDT) is essential in the management of cancer. Head and neck cancer (HNC) is a rare, complex and heterogeneous group of malignancies for which different treatment options are available. However, the potential impact of MDT on the management of HNC has been only poorly evaluated to ...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2016.01.001
更新日期:2016-03-01 00:00:00
abstract::A unique case of bilateral mucoepidermoid carcinoma arising in pre-existing bilateral Warthin's tumours of the parotid glands is presented. Reports of carcinomas arising in pre-existing unilateral Warthin's tumours are likewise rare with only 3 cases of concern mucoepidermoid carcinoma. This is the first report of bil...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/s0964-1955(97)00018-3
更新日期:1997-07-01 00:00:00
abstract::Squamous cell carcinoma of the oral cavity (SCC) accounts for 3% of cancers in the western world and 40% of cancers in India. The overall 5-year survival rate is only 50%. Most of the lesions appear intra-orally on the tongue. Results from a previous study demonstrated a significant increase in T and B-lymphocytes und...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2003.08.008
更新日期:2004-03-01 00:00:00
abstract:PURPOSE:To describe patterns of failure (POF) after reirradiation (reRT) with intensity modulated radiation therapy (IMRT) for recurrent/second primary squamous cell carcinoma of the head and neck. METHODS:From 08/2004-02/2013, 75 consecutive patients received reRT with IMRT. Gross tumor was generally treated with a 5...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2016.07.012
更新日期:2016-10-01 00:00:00
abstract:UNLABELLED:Squamous cell carcinoma of the tongue (TSCC) has one of the poorest prognoses of head and neck cancers. This study aims to improve early detection of the disease by identifying salivary biomarkers that can identify a spectrum of patients progressing from high-risk to TSCC. We also examine the mortality of ex...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2011.02.006
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVES:Diffusion weighted imaging (DWI) is a frequently performed MRI sequence in cancer patients. While previous studies have shown the clinical value of the apparent diffusion coefficient (ADC) for response prediction and response monitoring, less is known about the biological background of ADC. In the tumor micr...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2017.12.001
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVES:The advantages of the circulating cell-free DNA (cfDNA) methodology are quick results and the possibility of repeated analysis. The main aim of our study was to establish the relationship of the total cfDNA with patients' clinical characteristics and circulating HPV DNA detection in the blood of patients wit...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2015.12.002
更新日期:2016-03-01 00:00:00
abstract::Odontogenic keratocyst (OKC) has an aggressive clinical course and a high tendency toward recurrence, while orthokeratinized odontogenic cyst has different characteristics and does not show aggressive behavior. Bax and bcl-2 are two important anti-apoptotic and pro-apoptotic factors of the bcl-2 family. The different ...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2008.12.007
更新日期:2009-07-01 00:00:00
abstract::The recently identified single nucleotide polymorphism in the MDM2 promoter (SNP 309) may contribute to the early onset of both sporadic and hereditary malignancies in patients with defective p53. We tested this hypothesis by examining the effects of combined MDM2 polymorphisms and somatic p53 mutations on 351 oral sq...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2008.03.006
更新日期:2009-01-01 00:00:00