Abstract:
:We report the cloning of cDNAs encoding two different human nonmuscle myosin heavy chains designated NMMHC-A and NMMHC-B. The mRNAs encoding NMMHC-A and NMMHC-B are both 7.5 kb in size but are shown to be the products of different genes, which are localized to chromosome 22q11.2 and chromosome 17q13, respectively. In aggreement with previously reported results using avian tissues, we show that the mRNAs encoding the two myosin heavy chain isoforms are differentially expressed in rat nonmuscle and muscle tissues as well as in a number of human cell lines. The cDNA sequence encoding the 5' portion of the NMMHC-A isoform completes the previously published 3' cDNA sequence encoding a human myosin heavy chain, thus providing the cDNA sequence encoding the entire NMMHC-A amino acid sequence. Comparison of this sequence to cDNA clones encoding the amino-terminal one third of the NMMHC-B sequence (amino acids 58-718) shows them to be 89% identical at the amino acid level and 74% identical at the nucleotide level.
journal_name
Circ Resjournal_title
Circulation researchauthors
Simons M,Wang M,McBride OW,Kawamoto S,Yamakawa K,Gdula D,Adelstein RS,Weir Ldoi
10.1161/01.res.69.2.530subject
Has Abstractpub_date
1991-08-01 00:00:00pages
530-9issue
2eissn
0009-7330issn
1524-4571journal_volume
69pub_type
杂志文章abstract::This report evaluates pulse wave propagation with respect to contributions by vascular wall elastic and geometric properties, vessel wall and blood viscosity, and nonlinearities in system parameters and in the equations of motion. Discrepancies in results obtained with different experimental methods and theory are dis...
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