Abstract:
:Until now, the effects of phytoestrogen on bone in both women and ovarian hormone-deficient animal models of osteoporosis have remained uncertain. We have aimed here to investigate the effect of genistein (GEN) on trabecular bone quality in ovariectomized (OVX) rats. Forty 7-month-old female Sprague-Dawley rats were randomly divided into the following four groups: OVX, sham-operated (SHAM), treated with 17beta-estradiol (EST, 10 microg x kg(-1) x day(-1)), and GEN (5 mg x kg(-1) x day(-1)). At 15 weeks postoperation, the compressive test was performed on the L5 vertebral body; additionally, microcomputed tomography (micro-CT) assessment was performed to estimate the bone mineral density (BMD) and microstructure parameters of the L6 vertebral body. After fatigue damage testing, the L6 vertebral body was bulk-stained in 1% basic fuchsin and embedded in methylmethacrylate. The L4 vertebral body was embedded in methylmethacrylate for dynamic histomorphometry analysis without staining. Mounted bone slices were used to measure microcrack parameters, empty osteocyte lacuna density (e.Lc.Dn), and osteocyte density (Ot.N/T.Ar). Maximum loading (ML) and Ot.N/T.Ar were significantly lower in the OVX group than in the other groups. E.Lc.Dn was significantly decreased in GEN and EST groups compared to the OVX group. ML was significantly decreased in the GEN group compared to the SHAM group. Microcrack density, microcrack surface density, and microcrack length were significantly increased in the OVX group compared to the other groups. Mineral apposition rate was significantly decreased in the OVX group compared to the SHAM and GEN groups. Bone formation rate was significantly decreased in the OVX group compared to other groups. There were no significant differences with regard to mineralizing surface among the four groups. Volumetric BMD at organ was significantly lower in OVX, EST, and GEN groups than in the SHAM group. Bone mineral content was significantly lower in the OVX group than in the SHAM group. Bone volume fraction and trabecular number were significantly decreased in OVX, EST, and GEN groups compared to the SHAM group. Structure model index was significantly lower in the SHAM group than in OVX, EST, and GEN groups. Trabecular separation was significantly increased in the OVX group compared to SHAM and EST groups. There were no significant differences with regard to the trabecular thickness (Tb,Th) between SHAM, GEN, and OVX groups. Tb.Th was significantly lower in the EST group than in the SHAM group. Connectivity density (Conn.D) was significantly lower in the OVX group than in SHAM and GEN groups, and Conn. D was significantly lower in the EST group than in GEN. In conclusion, the present study demonstrates that GEN preserved the biomechanical quality of the trabecular bone regardless of the microstructure and BMD.
journal_name
J Bone Miner Metabjournal_title
Journal of bone and mineral metabolismauthors
Dai R,Ma Y,Sheng Z,Jin Y,Zhang Y,Fang L,Fan H,Liao Edoi
10.1007/s00774-007-0830-4subject
Has Abstractpub_date
2008-01-01 00:00:00pages
342-9issue
4eissn
0914-8779issn
1435-5604journal_volume
26pub_type
杂志文章abstract::There is no well-established treatment for osteoporosis in male patients with leprosy, because no clinical trials have examined the efficacy of treatment on bone mineral density (BMD) or fracture incidence in patients with leprosy. In this study, we report a case of osteoporosis in a man with leprosy, treated by oral ...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-004-0546-7
更新日期:2005-01-01 00:00:00
abstract::Data from retrospective and prospective observational and case-control studies suggest that activity is associated with reduced fracture risk, but consistently replicated bias may be responsible for this desired endpoint. Exercise during growth is likely to build a larger and stronger skeleton. However, cessation of e...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章,评审
doi:10.1007/BF03026319
更新日期:2005-01-01 00:00:00
abstract::Nitrogen-containing bisphosphonates such as zoledronic acid (ZOL) and pamidronate have been widely and successfully used for the treatment of cancer patients with bone metastases and/or hypercalcemia. Accumulating recent reports have shown that cancer patients who have received these bisphosphonates occasionally manif...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-009-0128-9
更新日期:2010-03-01 00:00:00
abstract::Gastrectomy-evoked osteopenia in the femoral metaphysis of rats can be prevented by the consumption of fructooligosaccharides (FOS). We examined the effect of FOS on the femoral neck. Twenty-eight 5-week-old male Sprague-Dawley rats were divided into two groups, sham-operated (SH) and gastrectomized (GX). One week aft...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-003-0423-9
更新日期:2003-01-01 00:00:00
abstract::Serum 25-hydroxyvitamin D (25-OHD) concentrations are thought to accurately reflect vitamin D stores, and vitamin D deficiency causes secondary hyperparathyroidism, irreversible bone loss, and increased risk of fracture. Recent studies suggest that decrease of serum 25-OHD level in mothers could increase the risk of p...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-011-0264-x
更新日期:2011-09-01 00:00:00
abstract::Various animal experiments and human studies have shown that intermittent injections of parathyroid hormone (PTH) exert anabolic effects on bone, whereas continuous PTH treatment decreases the bone mass and causes hypercalcemia in animals. However, limited data are available with regard to the effects of a repetitive ...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-010-0181-4
更新日期:2010-11-01 00:00:00
abstract::Expression of the genes encoding bone morphogenetic proteins (BMPs), BMP type IA receptor (BMPR-1A), and rat distal-less homolog (rDlx) was studied in bone, callus, and the surrounding soft tissue following rat femoral closed fracture, using RT-PCR-based techniques. Before fracture, the genes encoding BMP-5, BMP-6, an...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s007740050013
更新日期:2000-01-01 00:00:00
abstract::We investigated the enhancement of new bone |formation elicited ectopically by recombinant human bone morphogenetic protein-2 (rhBMP-2), using parathyroid hormone (PTH) and a phosphodiesterase inhibitor (PDEi), pentoxifylline (PTX), in an animal model. Collagen sponge sheet discs containing rhBMP were implanted onto t...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-003-0490-y
更新日期:2004-01-01 00:00:00
abstract::Aromatase inhibitors (AIs) are the gold standard therapy for breast cancer in postmenopausal women. AI suppresses the conversion of androgens to estrogens; however, this results in osteopenia, osteoporosis, and bone fracture, thus reducing the patient's quality of life. The use of adjuvant denosumab reduces the risk o...
journal_title:Journal of bone and mineral metabolism
pub_type: 临床试验,杂志文章
doi:10.1007/s00774-018-00985-8
更新日期:2019-09-01 00:00:00
abstract::Chronic kidney disease (CKD) mineral and bone disorders (CKD-MBD) may lead to low bone mineral density (BMD) and vascular calcification (VC), but links to the latter are unclear. Here we investigated associations between BMD, coronary artery calcium (CAC) scores, and histological signs of VC in end-stage renal disease...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-016-0788-1
更新日期:2017-09-01 00:00:00
abstract::Lipid rafts are membrane platforms for signaling molecules that regulate various cellular functions. During osteoclastogenesis, lipid rafts also have been shown to play a crucial role for cell fusion upon RANK/RANKL interaction and further activation of osteoclasts, resulting in bone resorption. To investigate protein...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-009-0150-y
更新日期:2010-07-01 00:00:00
abstract::Advanced glycation end products (AGE) in collagen have been reported to decrease the mechanical property of bone. However, there are no available data on the relation between fracture risk and levels of glycoxidative (nonenzymatic) cross-links of collagen in clinical samples. A total of 432 Japanese elderly women who ...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-007-0784-6
更新日期:2008-01-01 00:00:00
abstract::Concurrent use of bisphosphonate therapy reduces the anabolic effect of teriparatide. Consequently, in clinical practice bisphosphonates are discontinued and teriparatide therapy held for a few months to allow bone turnover to increase. We aimed to evaluate the effect of prior bisphosphonate exposure and the effect of...
journal_title:Journal of bone and mineral metabolism
pub_type: 临床试验,杂志文章
doi:10.1007/s00774-009-0101-7
更新日期:2010-01-01 00:00:00
abstract::Metabolic bone disease may appear as a complication of obesity surgery. Because an imbalance in the osteoprotegerin and receptor-activator of nuclear factor-κB ligand system may underlie osteoporosis, we aimed to study this system in humans in the metabolic bone disease occurring after obesity surgery. In this study w...
journal_title:Journal of bone and mineral metabolism
pub_type: 临床试验,杂志文章
doi:10.1007/s00774-015-0712-0
更新日期:2016-11-01 00:00:00
abstract::Vertebral fractures are the most common complication associated with osteoporosis. The definition of prevalent or incident vertebral fractures is important in epidemiological evaluation and in clinical trials of osteoporosis treatment. There have been few reports regarding the morphometric cutoff criteria for incident...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-003-0457-z
更新日期:2004-01-01 00:00:00
abstract::The aim of this observational, non-randomized study was to clarify the unknown effects of switching oral bisphosphonates (BPs) to denosumab (DMAb) or daily teriparatide (TPTD) in patients with rheumatoid arthritis (RA). The characteristics of the 194 female patients included in the study were 183 postmenopausal, age 6...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章,多中心研究
doi:10.1007/s00774-017-0861-4
更新日期:2018-07-01 00:00:00
abstract::Recent studies have suggested that a high level of recent physical activity increases the risk of a wrist fracture in postmenopausal women. The relationship of more distant past physical activity to wrist fracture is less clear, and most studies have relied on recall of physical activity much earlier in life. The aim ...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-006-0715-y
更新日期:2006-01-01 00:00:00
abstract::This study aimed to estimate the prevalence of locomotive syndrome, sarcopenia, and frailty and clarify their co-existence in a population-based cohort. The third survey of Research on Osteoarthritis/Osteoporosis Against Disability (ROAD) study was conducted between 2012 and 2013, examining 963 subjects (aged ≥ 60 yea...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-019-01012-0
更新日期:2019-11-01 00:00:00
abstract::The G171V mutation (high bone mass, HBM) is autosomal dominant and is responsible for high bone mass in humans. Transgenic HBM mice in which the human LRP5 G171V gene is inserted also show a similar phenotype with greater bone mass and biomechanical performance than wild-type mice, as determined by whole bone testing....
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-010-0194-z
更新日期:2011-01-01 00:00:00
abstract::Bone development up to early adulthood plays an important role in determining the risk of osteoporosis later in life. However, bone development in children has not been fully documented by longitudinal studies in Japanese children. The purpose of this study is to determine the degree of tracking of areal bone mineral ...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-010-0213-0
更新日期:2011-03-01 00:00:00
abstract::The aim of this study was to explore the effects of α-zearalanol (α-ZAL) on the proliferation of mouse bone-marrow-derived mesenchymal stem cells (BMSCs) and their differentiation into osteoblasts. Six- to eight-week-old BALB/C mice were used either as recipients or as bone marrow donors. BMSCs were isolated and colle...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-015-0659-1
更新日期:2016-03-01 00:00:00
abstract::This study aimed to identify the osteogenesis effect of icariside II (ICSII) and icaritin (ICT) in vitro. Bone marrow mesenchymal stem cells (BMSCs) were treated with ICSII and ICT in order to detect the proliferation and differentiation of BMSCs, the expression of the osteogenesis-related proteins with or without ost...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-017-0889-5
更新日期:2018-11-01 00:00:00
abstract::Bone microarchitecture of the iliac bone is used to characterize the properties of bone tissue in osteoporosis, particularly in pharmacological studies. Trabecular bone is known to be heterogeneous media. For a few years, the analysis of three-dimensional (3-D) bone microarchitecture has been based on micro-computed t...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-008-0856-2
更新日期:2008-01-01 00:00:00
abstract::Notch is a transmembrane protein involved in cell fate determination. In the present study, we observed temporally and spatially restricted expression of Notch1 in developing cartilage. Notch1 was localized starting from the mesenchymal condensation stage of embryonic mouse forelimbs. Interestingly, although localizat...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-003-0428-4
更新日期:2003-01-01 00:00:00
abstract:INTRODUCTION:Etelcalcetide binds to the extracellular domain of the calcium-sensing receptor (CaSR), while cinacalcet binds to the 7-transmembrane domain of the CaSR; however, it is unknown, whether etelcalcetide has similar effects to cinacalcet on parathyroid hormone (PTH) secretion. MATERIALS AND METHODS:The PTH-ca...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-020-01169-z
更新日期:2020-11-16 00:00:00
abstract::Senile or age-related/dependent osteoporosis is caused by reduced bone formation, rather than increased bone resorption as in postmenopausal osteoporosis. Here we review genetically engineered mouse models with defects in osteoblastic proliferation or differentiation with focus on IGF signaling and stem cells. Model m...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章,评审
doi:10.1007/s00774-004-0517-z
更新日期:2004-01-01 00:00:00
abstract::In 50 premenarcheal girls selected from the lowest and highest end of the calcium-intake distribution of a large population sample, we evaluated bone mineral density (BMD), together with the following hormonal-metabolic parameters: androstenedione (ASD), dehydroepiandrosterone (DHEA), dehydroepiandrosterone sulfate (D...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-003-0451-5
更新日期:2004-01-01 00:00:00
abstract::In order to explore the effects of calcitonin gene-related peptide (CGRP) on bone mesenchymal stem cells (BMSCs) from ovariectomized (OVX) rats, an OVX rat model was used. An ELISA was performed to examine the changes in CGRP level in the plasma and skeleton. The BMSCs from the sham rats were designated group A. The B...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-016-0780-9
更新日期:2017-09-01 00:00:00
abstract::Bone mass differs according to ethnic classification, with individuals of African ancestry attaining the highest measurements across numerous skeletal sites. Elevated bone mass is even maintained in those individuals exposed to adverse environmental factors, suggesting a prominent genetic effect that may have clinical...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-013-0431-3
更新日期:2013-11-01 00:00:00
abstract::Bone mineral density (BMD) and peak bone mass (PBM) are important determinants of skeletal resistance. The development of bone densitometry improved the possibility of studying BMD and the influence of genetic and environmental factors on bone. Heredity factors are important for BMD, and Runx-2 is accepted as a regula...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-012-0373-1
更新日期:2012-11-01 00:00:00