Lactase production by solid-state cultivation of Kluyveromyces marxianus CDBBL 278 on an inert support: effect of inoculum, buffer, and nitrogen source.

abstract：:Tomato pomace is an abundant lignocellulosic waste stream from industrial tomato processing and therefore a potential feedstock for production of renewable biofuels. However, little research has been conducted to determine if pretreatment can enhance release of fermentable sugars from tomato pomace. Ionic liquids (ILs...

journal_title：Applied biochemistry and biotechnology

authors： Allison BJ,Cádiz JC,Karuna N,Jeoh T,Simmons CW

更新日期：2016-08-01 00:00:00

abstract：:Development and application of a system for real-time quantitative assessment of individual cell activities in a mixed culture system was investigated. This was based on a concept that the activities of individual cells in a mixed culture can be assessed if the cells are physically separated (in separate compartments)...

journal_title：Applied biochemistry and biotechnology

authors： Tanaka H,Ebata T,Kuwahara I,Matsuo M,Ogbonna JC

更新日期：1999-04-01 00:00:00

abstract：:The ethanologenic bacteria Escherichia coli strains KO11 and LYO1, and Klebsiella oxytoca strain P2, were investigated for their ability to metabolize furfural. Using high performance liquid chromatography and 13C-nuclear magnetic resonance spectroscopy, furfural was found to be completely biotransformed into furfuryl...

journal_title：Applied biochemistry and biotechnology

authors： Gutiérrez T,Buszko ML,Ingram LO,Preston JF

更新日期：2002-04-01 00:00:00

abstract：:(R)-3, 5-Bis(trifluoromethyl)phenyl ethanol is a key chiral intermediate for the synthesis of aprepitant. Through a genome mining approach, an NADPH-dependent short-chain dehydrogenases derived from Burkholderia cenocepacia (Bc-SDR) was discovered with excellent anti-Prelog's stereoselectivity of reducing 3, 5-bis(tri...

journal_title：Applied biochemistry and biotechnology

authors： Yu S,Li H,Lu Y,Zheng G

更新日期：2018-04-01 00:00:00

abstract：:Laccase is one of the lignolytic enzymes found in liquid cultures of the fungus Coriolus zonatus in defined medium. The enzyme was isolated from culture liquid and characterized. Laccase from C. zonatus is a single-chain protein with a molecular mass of 60 kDa. Carbohydrate moiety of enzyme consisted of mannose, galac...

journal_title：Applied biochemistry and biotechnology

authors： Koroljova OV,Stepanova EV,Gavrilova VP,Biniukov VI,Jaropolov AI,Varfolomeyev SD,Scheller F,Makower A,Otto A

更新日期：1999-02-01 00:00:00

abstract：:A 28-day feeding experiment with formulated feed using docosahexaenoic acid (DHA)-rich whole cells of freeze-dried marine microalgae Schizochytrium sp. to understand the distribution of fatty acids in a laboratory model zebrafish was conducted. Three feeds, commercial feed, 50:50 feed (50% commercial and 50% algae), a...

journal_title：Applied biochemistry and biotechnology

authors： Byreddy AR,Yoganantharjah P,Gupta A,Gibert Y,Puri M

更新日期：2019-05-01 00:00:00

abstract：:Gluconacetobacter spp. synthesize a pure form of hydrophilic cellulose that has several industrial specialty applications. Literature reports have concentrated on intensive investigation of static and agitated culture in liquid media containing high nutrient concentrations optimized for maximal cellulose production ra...

journal_title：Applied biochemistry and biotechnology

authors： Evans BR,O'Neill HM

更新日期：2005-04-01 00:00:00

abstract：:Proinflammatory cytokines such as tumor necrosis factor (TNF) alpha are well known to inhibit adipocyte differentiation. TNF-alpha triggers ceramide synthesis through binding of TNF-alpha to its p55 receptor. Therefore, ceramide is implicated in many of the multiple signaling pathways initiated by TNF-alpha. In breast...

journal_title：Applied biochemistry and biotechnology

authors： Xu F,Yang CC,Gomillion C,Burg KJ

更新日期：2010-01-01 00:00:00

abstract：:The increase of multidrug-resistant pathogens and the restriction on the use antibiotics due to its side effects have drawn attention to the search for possible alternatives. Bacteriocins are small antimicrobial peptides produced by numerous bacteria. Much interest has been focused on bacteriocins because they exhibit...

journal_title：Applied biochemistry and biotechnology

authors： Jena PK,Trivedi D,Chaudhary H,Sahoo TK,Seshadri S

更新日期：2013-04-01 00:00:00

abstract：:In a recombinant antibody scFv format, the presence of an unpaired cysteine (Cys) is implicated in reduced soluble expression and inefficient presentation in phage display. Compared to other species, antibodies derived from rabbits are more likely to contain this unpaired Cys residue at position 80 (Cys80), when gener...

journal_title：Applied biochemistry and biotechnology

authors： Ayyar BV,Hearty S,O'Kennedy R

更新日期：2018-07-01 00:00:00

abstract：:The pLKO.1-TRC plasmid has been a popular and widely used vector due to its simple handling and stability. The huge RNAi database, a TRC library, has been established based on this vector. However, this plasmid only has a puromycin-resisted gene for selecting, which limits its application in microscopy and fluorescenc...

journal_title：Applied biochemistry and biotechnology

authors： Xu J,Zhu W,Guo Y,Jiang C,Hussain N,Meng L,Lu S

更新日期：2017-02-01 00:00:00

abstract：:A microbial cyanide sensor was prepared, consisting of immobilized Saccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of the microorganisms increased. The change in the respiration activity is monitored with the oxygen electrod...

journal_title：Applied biochemistry and biotechnology

authors： Nakanishi K,Ikebukuro K,Karube I

更新日期：1996-08-01 00:00:00

abstract：:Polygalacturonases are part of the group of enzymes involved in pectin degradation. The aim of this work was to investigate some of the factors affecting polygalacturonase production by an Aspergillus giganteus strain and to characterize this pectinolytic activity. Several carbon sources, both pure substances and natu...

journal_title：Applied biochemistry and biotechnology

authors： Pedrolli DB,Gomes E,Monti R,Carmona EC

更新日期：2008-02-01 00:00:00

abstract：:The application of immunoaffinity techniques to subcellular fractionation is reviewed and the basic principles underlying the various methods that have been successfully employed, identified. The requirement for organelle-specific antigens and high-avidity antibodies is discussed, as is the widespread use of indirect ...

journal_title：Applied biochemistry and biotechnology

authors： Richardson PJ,Luzio JP

更新日期：1986-10-01 00:00:00

abstract：:The ability of non-living biomass of an arsenic-hypertolerant Bacillus cereus strain SZ2 isolated from a gold mining environment to adsorb As (III) from aqueous solution in batch experiments was investigated as a function of contact time, initial As (III) concentration, pH, temperature and biomass dosage. Langmuir mod...

journal_title：Applied biochemistry and biotechnology

authors： Mohd Bahari Z,Ali Hamood Altowayti W,Ibrahim Z,Jaafar J,Shahir S

更新日期：2013-12-01 00:00:00

abstract：:Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation. Presence of silic...

journal_title：Applied biochemistry and biotechnology

authors： Krishnamoorthy S,Iyer NR,Murthy AR

更新日期：2015-02-01 00:00:00

abstract：:Agrobacterium isolated from soil samples produced two extracellular polysaccharides: succinoglycan, an acidic soluble polymer, and curdlan gum, a neutral, insoluble polymer. Maize glucose, cassava glucose, and maize maltose were used in fermentation medium to produce insoluble polysaccharide. Two Agrobacterium sp. str...

journal_title：Applied biochemistry and biotechnology

authors： Portilho M,Matioli G,Zanin GM,de Moraes FF,Scamparini AR

更新日期：2006-03-01 00:00:00

abstract：:Reed plants (Phragmites communis (Linn.) Trin) are hydrophilic perennial grasses growing in fresh and brackish waters. These plants readily adapt to arid and high salinity conditions; however, their resistance mechanism against abiotic stresses, especially high salinity, is largely unknown. In the present study, we cl...

journal_title：Applied biochemistry and biotechnology

authors： Zhang X,Quan G,Wang J,Han H,Chen S,Guo S,Yin H

更新日期：2015-04-01 00:00:00

abstract：:The objective of this study was to understand the effect of pH and ionic strength of aluminum sulfate on the flocculation of microalgae. It was found that changing pH and ionic strength influenced algal flocculation by changing the zeta potential of cells, which was described by the classical theory of Derjaguin, Land...

journal_title：Applied biochemistry and biotechnology

authors： Cui Y,Yuan W,Cheng J

更新日期：2014-08-01 00:00:00

abstract：:Alternative oxidase (AOX) is a nonproton motive quinol-oxygen oxidoreductase which is a component of the mitochondrial respiratory chain in higher plants. In this study, we have characterized the catalytic activity and regulatory behaviors of Arum concinnatum AOX isoforms, namely AcoAOX1a and AcoAOX1b, and their artif...

journal_title：Applied biochemistry and biotechnology

authors： Kakizaki Y,Ito K

更新日期：2013-07-01 00:00:00

abstract：:In the present work, assays were performed to compare the efficacy of hypochlorous acid, chlorine dioxide, and ozone in the inactivation of Cryptosporidium oocyst in public water supply from Brazilian South conditions. Experiments were carried out in samples containing 2 x 10(4) oocysts/ml of C. parvum purified from f...

journal_title：Applied biochemistry and biotechnology

authors： Pereira JT,Costa AO,de Oliveira Silva MB,Schuchard W,Osaki SC,de Castro EA,Paulino RC,Soccol VT

更新日期：2008-12-01 00:00:00

abstract：:Sorghum was pretreated by sole ultrasound or supercritical carbon dioxide (scCO2), as well as the method combining both to intensify enzymatic hydrolysis. The effect of the time (1-5 h) and temperature (30-70 °C) on ultrasonic pretreatment was investigated, and the best condition was determined as 5 h and 50 °C with t...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Q,Zhao M,Xu Q,Ren H,Yin J

更新日期：2019-05-01 00:00:00

abstract：:An aspartic protease gene from Pleurotus ostreatus (Po-Asp) had been cloned based on the 3' portion of cDNA in our previous work. The Po-Asp cDNA contained 1,324 nucleotides with an open reading frame (ORF) of 1,212 bp encoding 403 amino acid residues. The putative amino acid sequence included a signal peptide, an act...

journal_title：Applied biochemistry and biotechnology

authors： Yin C,Zheng L,Chen L,Tan Q,Shang X,Ma A

更新日期：2014-02-01 00:00:00

abstract：:Multi-strain mixed fermentation can provide a relatively complete lignocellulosic enzyme system compared with single-strain fermentation. This study was firstly to screen strains which have a strong ability to hydrolyse rice straw (RS) enzymatically and enrich true protein (TP). Then, the conditions in the process of ...

journal_title：Applied biochemistry and biotechnology

authors： Jia J,Chen H,Wu B,Cui F,Fang H,Wang H,Ni Z

更新日期：2019-01-01 00:00:00

abstract：:Milk is a natural nutraceutical produced by mammals. The nanovesicles of milk play a role in horizontal gene transfer and confer health-benefits to milk consumers. These nanovesicles contain miRNA, mRNA, and proteins which mediate the intercellular communication. In this work, we isolated and characterized the buffalo...

journal_title：Applied biochemistry and biotechnology

authors： Baddela VS,Nayan V,Rani P,Onteru SK,Singh D

更新日期：2016-02-01 00:00:00

abstract：:Superoxide dismutase (SOD, EC 1.15.1.1) is an enzyme catalyzing the dismutation of superoxide radical to hydrogen peroxide and dioxygen. To date, four types of SODs--Cu/ZnSOD, MnSOD, FeSOD, and NiSOD--have been identified. In this study, SOD proteins of Brachypodium distachyon (L.) Beauv. were screened by utilization ...

journal_title：Applied biochemistry and biotechnology

authors： Filiz E,Koc I,Ozyigit II

更新日期：2014-07-01 00:00:00

abstract：:We expressed a putative beta-galactosidase from Sulfolobus acidocaldarius in Escherichia coli and purified the recombinant enzyme using heat treatment and Hi-Trap ion-exchange chromatography. The resultant protein gave a single 57-kDa band by SDS-PAGE and had a specific activity of 58 U/mg. The native enzyme existed a...

journal_title：Applied biochemistry and biotechnology

authors： Park AR,Kim HJ,Lee JK,Oh DK

更新日期：2010-04-01 00:00:00

abstract：:In the present study, the neuroprotection of osthole (OST) was confirmed. In L-glutamic acid (L-Glu)-damaged HT22 cells, a 3-h pre-incubation with OST-enhanced cell viability suppressed the apoptosis rate; inhibited the activities of caspase-3, caspase-8, and caspase-9; reduced the over-accumulation of intracellular r...

journal_title：Applied biochemistry and biotechnology

authors： Chu Q,Zhu Y,Cao T,Zhang Y,Chang Z,Liu Y,Lu J,Zhang Y

更新日期：2020-02-01 00:00:00

abstract：:Prot-2 protease previously purified to homogeneity from Botrytis cinerea showed potentiality to be used in detergency and for production of bioactive peptides. To extend the characterization of Prot-2 protease, antifungal and antibacterial assays were performed in vitro using protein hydrolysates prepared from muscle ...

journal_title：Applied biochemistry and biotechnology

authors： Abidi F,Aissaoui N,Gaudin JC,Chobert JM,Haertlé T,Marzouki MN

更新日期：2013-05-01 00:00:00

abstract：:(R)-2-Phenoxy-propionic acid methyl ester (PPAM) is an important chiral precursor of aryloxy phenoxy propionate herbicides. The covalent immobilization of lipase from Aspergillus oryzae WZ007 and the catalysis of enantioselective (R, S)-PPAM resolution by the immobilized A. oryzae lipase (AOL) were investigated in thi...

journal_title：Applied biochemistry and biotechnology

authors： Zhong W,Zhang M,Li X,Zhang Y,Wang Z,Zheng J

更新日期：2020-03-01 00:00:00