Detection of mutation in isoniazid-resistant Mycobacterium tuberculosis isolates from tuberculosis patients in Belarus.

Abstract:

:The aim of this study was to investigate the frequency, location and type of katG mutations in Mycobacterium tuberculosis strains isolated from patients in Belarus. Forty two isoniazid-resistant isolates were identified from sputum of 163 patients with active pulmonary tuberculosis. Drug susceptibility testing was determined by using CDC standard conventional proportional method and BACTEC system. Standard PCR method for detection of isoniazid resistance associated mutations was performed by katG gene amplification and DNA sequencing. Most mutations were found in katG gene codons 315, 316 and 309. Four types of mutations were identified in codon 315: AGC-->ACC (n=36) 85%, AGC-->AGG (n=1) 2.3%, AGC-->AAC (n=2) 4.7%, AGC-->GGC (n=1) 2.3%. One type of mutation was found in codon 316: GGC-->AGC (n=18) 41.4%, four types of mutations were detected in codon 309: GGT-->GGT (n=7) 16.1%, GGT-->GCT (n=4) 9.2%, GGT-->GTC (n=3)6.9%, GGT-->GGG (n=1) 2.7%. The highest frequency of mutations sharing between primary and secondary infections was found in codon 315.

journal_name

Indian J Med Microbiol

authors

Bostanabad SZ,Titov LP,Bahrmand A,Nojoumi SA

doi

10.4103/0255-0857.40528

subject

Has Abstract

pub_date

2008-04-01 00:00:00

pages

143-7

issue

2

eissn

0255-0857

issn

1998-3646

journal_volume

26

pub_type

杂志文章
  • Treatment of discarded blood units: disinfection with hypochlorite / formalin versus steam sterilization.

    abstract::Blood bank regulations and bio medical waste rules of India advocate disinfection of contaminated blood units. Incineration is not recommended due to poly-vinyl chloride (PVC) content of blood bags. This study was designed to evaluate the efficacy of chemical disinfection of blood units deliberately contaminated with ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Chitnis V,Chitnis S,Patil S,Chitnis D

    更新日期:2003-10-01 00:00:00

  • High prevalence of hepatitis A virus antibody among Bangladeshi children and young adults warrants pre-immunization screening of antibody in HAV vaccination strategy.

    abstract::Serum samples from 465 subjects aged between 1 and 25 years were tested for antibody against hepatitis A virus (HAV) [anti-HAV IgG and IgM] to determine the seroprevalence of HAV antibody and do a cost-benefit analysis for decision making about vaccination against HAV among the general population of Bangladesh. A high...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Ahmed M,Munshi SU,Nessa A,Ullah MS,Tabassum S,Islam MN

    更新日期:2009-01-01 00:00:00

  • Detection of porcine rotavirus from tissue and faecal specimens.

    abstract::Porcine small intestinal sub-mucosa is a cell-free collagen matrix that has demonstrated its ability as a scaffold material. Transplantation poses special hazards because grafted tissues and organs transmit pathogens efficiently, especially viruses. Rotavirus is thought to be confined to the intestine, causing acute d...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.49430

    authors: Prabha S,Verghese S

    更新日期:2009-04-01 00:00:00

  • Identification of opportunistic enteric parasites among immunocompetent patients with diarrhoea from Northern India and genetic characterisation of Cryptosporidium and Microsporidia.

    abstract:PURPOSE:Enteric parasitic infestation is a major public health problem in developing countries. Parasites such as Cryptosporidium spp., Cyclospora spp., Cystoisospora spp. and Microsporidia may cause severe diarrhoea among immunocompromised patients. There is scanty data on their frequency among immunocompetent patient...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.174114

    authors: Ghoshal U,Dey A,Ranjan P,Khanduja S,Agarwal V,Ghoshal UC

    更新日期:2016-01-01 00:00:00

  • Investigation of an outbreak of varicella in Chandigarh, North India, using a real-time polymerase chain reaction approach.

    abstract::Outbreaks of varicella are reported when susceptible population accumulates. This study reports a chickenpox outbreak in Burail in August 2014, wherein 20 laboratory-confirmed cases were identified by the detection of varicella zoster virus (VZV) DNA and VZV IgM antibodies. The viral load between vesicular swabs and s...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_16_420

    authors: Singh MP,Kaur R,Kumar A,Gupta M,Garg S,Ratho RK

    更新日期:2017-07-01 00:00:00

  • A comparative study for the detection of Mycobacteria by BACTEC MGIT 960, Lowenstein Jensen media and direct AFB smear examination.

    abstract:PURPOSE:To compare BACTEC MGIT 960 (M960) with conventional culture on Lowenstein Jensen (LJ) media and direct acid fast bacilli (AFB) smear examination for the detection of Mycobacteria in clinical samples obtained from suspected cases of pulmonary and extra pulmonary tuberculosis (TB). METHODS:A total of 500 samples...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.37344

    authors: Rishi S,Sinha P,Malhotra B,Pal N

    更新日期:2007-10-01 00:00:00

  • Primary meningococcal arthritis of sacroiliac joint: a rare case report.

    abstract::Infection of the sacroiliac joint is a rare entity. Clinical signs and symptoms are usually nonspecific and result in delayed diagnosis. We report a rare case of primary meningococcal arthritis of right sacroiliac joint in an 11-year-old male child. Synovial fluid aspirated from the joint space showed Gram-negative di...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.108743

    authors: Sahu S,Mohanty I,Narasimham MV,Pasupalak S,Parida B

    更新日期:2013-01-01 00:00:00

  • Coagulase-negative staphylococci causing blood stream infection at an Indian tertiary care hospital: Prevalence, antimicrobial resistance and molecular characterisation.

    abstract:INTRODUCTION:Recent years have seen a rise of coagulase-negative staphylococci (CoNS) from common contaminants to agents of nosocomial blood stream infections (BSI's). Molecular typing and establishing a correlation with antibiotic resistance is essential particularly in countries like India where genotyping studies fo...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.195374

    authors: Singh S,Dhawan B,Kapil A,Kabra SK,Suri A,Sreenivas V,Das BK

    更新日期:2016-10-01 00:00:00

  • Colistin susceptibility testing of gram-negative bacilli: Better performance of vitek2 system than E-test compared to broth microdilution method as the gold standard test.

    abstract:Introduction:Unavailability of optimal susceptibility testing (ST) challenges the clinical use of colistin. Broth microdilution (BMD), which is the reference for colistin ST, is inconvenient for diagnostics. Vitek2 and E-test although technically easier, are no longer recommended. Materials and Methods:For the evaluat...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_19_480

    authors: Das S,Roy S,Roy S,Goelv G,Sinha S,Mathur P,Walia K,Bhattacharya S

    更新日期:2020-01-01 00:00:00

  • A study of tubercular lymphadenitis: a comparison of various laboratory diagnostic modalities with a special reference to tubercular polymerase chain reaction.

    abstract:OBJECTIVE:The purpose of our study was to compare various laboratory diagnostic methods, namely histopathological examination, Ziehl-Neelsen (ZN) stain, AFB culture by conventional Lowenstein-Jensen (LJ) method and fluorescence-based mycobacterial growth indicator tube (MGIT) technique and polymerase chain reaction (PC...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.90173

    authors: Patwardhan SA,Bhargava P,Bhide VM,Kelkar DS

    更新日期:2011-10-01 00:00:00

  • Fatal missed case of hemophagocytic lymphohistiocytosis co-infected with parvovirus B19 and Epstein-Barr virus in an infant: test hyperferritinaemia early.

    abstract::Hemophagocytic lymphohistiocytosis (HLH) triggered by Parvovirus B19 and Epstein-Barr virus co-infection is rare and unknown in infants. A 2-month-old male infant with fever, rash, bicytopenia and hepato-splenomegaly died owing to diagnostic dilemmas. Hence simply testing for hyperferritinaemia and hypertriglyceridemi...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.129819

    authors: Kishore J,Kishore D

    更新日期:2014-04-01 00:00:00

  • Changing trends of culture-positive typhoid fever and antimicrobial susceptibility in a tertiary care North Indian Hospital over the last decade.

    abstract:Purpose:The present study was undertaken to analyse the trend in prevalence of culture-positive typhoid fever during the last decade and to determine antimicrobial susceptibility profile of Salmonella Typhi and Salmonella Paratyphi A isolated from patients of enteric fever presenting to our hospital. Methods:All the c...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_17_412

    authors: Sharma P,Dahiya S,Manral N,Kumari B,Kumar S,Pandey S,Sood S,Das BK,Kapil A

    更新日期:2018-01-01 00:00:00

  • Comparison of various microbiological tests including polymerase chain reaction for the diagnosis of osteoarticular tuberculosis.

    abstract:PURPOSE:To evaluate the utility of the polymerase chain reaction (PCR) test for diagnosing osteoarticular tuberculosis (TB). METHODS:Clinical samples (synovial tissue and synovial fluid) obtained from 23 cases of suspected osteoarticular tuberculosis were subjected to Ziehl Neelsen (ZN) smear examination, radiometric ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Negi SS,Gupta S,Khare S,Lal S

    更新日期:2005-10-01 00:00:00

  • Ludwig's angina by Salmonella Typhi: a clinical dilemma.

    abstract::Salmonella Typhi has rarely been associated with focal abscesses; and in literature, there is no evidence of its association with abscesses in the neck spaces. Ability of Salmonella Typhi to invade and localise in the neck spaces not only poses a diagnostic challenge but also underscores the necessity to understand th...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.154889

    authors: Mahajan RK,Sharma S,Madan P,Sharma N

    更新日期:2015-04-01 00:00:00

  • Nanotechnology: a new frontier in virus detection in clinical practice.

    abstract::Researchers are expanding the applications of nanotechnology in the field of medicine since mid-2000. These technologies include nanoarrays, protein arrays, nanopore technology, nanoparticles as a contrivance in immunoassays and nanosensors, among others. Nanobiotechnologies are clinically applicable and possess the p...

    journal_title:Indian journal of medical microbiology

    pub_type: 社论,评审

    doi:

    authors: Abraham AM,Kannangai R,Sridharan G

    更新日期:2008-10-01 00:00:00

  • ESBL - from petri dish to the patient.

    abstract::Treatment of extended spectrum beta-lactamase (ESBL) producing strains of Enterobacteriaceae has emerged as a major challenge in hospitalised as well as community based patients. Infections due to ESBL producers range from uncomplicated urinary tract infection to life threatening sepsis. Although several reviews have ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.4103/0255-0857.19889

    authors: Bhattacharya S

    更新日期:2006-01-01 00:00:00

  • Chikungunya fever: a re-emerging viral infection.

    abstract::Chikungunya (CHIK) fever is a re-emerging viral disease characterized by abrupt onset of fever with severe arthralgia followed by constitutional symptoms and rash lasting for 1-7 days. The disease is almost self-limiting and rarely fatal. Chikungunya virus (CHIKV) is a RNA virus belonging to family Togaviridae, genus ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.4103/0255-0857.38850

    authors: Chhabra M,Mittal V,Bhattacharya D,Rana U,Lal S

    更新日期:2008-01-01 00:00:00

  • Safe transportation of biomedical waste in a health care institution.

    abstract:INTRODUCTION:The chances of health care waste (Biomedical waste) coming in contact with the health care workers, patients, visitors, sanitary workers, waste handlers, public, rag pickers and animals during transportation are high. MATERIALS AND METHODS:The study was conducted over a period of seven months (April 2013-...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.158559

    authors: Kumar A,Duggal S,Gur R,Rongpharpi SR,Sagar S,Rani M,Dhayal D,Khanijo CM

    更新日期:2015-07-01 00:00:00

  • Assessment of trends of ofloxacin resistance in Mycobacterium tuberculosis.

    abstract:PURPOSE:Ofloxacin (OFX) is one of the potent fluoroquinolone (FQ) recommended to treat MDR-TB. Over a decade, the preexposure of this drug for the treatment of other bacterial infections has resulted in acquisition of FQ resistance among Mycobacterium tuberculosis strains. Considering this possibility, a study was unde...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.83913

    authors: Verma JS,Nair D,Rawat D,Manzoor N

    更新日期:2011-07-01 00:00:00

  • Superantigens - concepts, clinical disease and therapy.

    abstract::The term superantigen was introduced in microbiology and immunology only a decade ago. The unique feature of superantigen is that it bypasses the antigen processing mechanism and specifically binds to TCR v segment and forms a trimolecular complex along with major histocompatibility complex class II. Since its discove...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Hemalatha V,Srikanth P,Mallika M

    更新日期:2004-10-01 00:00:00

  • Rapid identification of non-sporing anaerobes using nuclear magnetic resonance spectroscopy and an identification strategy.

    abstract:PURPOSE:The non-sporing anaerobes cause a wide spectrum of infections. They are difficult to culture and their identification is tedious and time-consuming. Rapid identification of anaerobes is highly desirable. Towards this end, the potential of nuclear magnetic resonance (NMR) spectroscopy for providing a fingerprint...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.37334

    authors: Menon S,Bharadwaj R,Chowdhary AS,Kaundinya DV,Palande DA

    更新日期:2007-10-01 00:00:00

  • Unimicrobial appendicitis due to non-vaccine serotype of streptococcus pneumoniae: Implications for and management and prevention.

    abstract::The aetiology of appendicitis is usually polymicrobial. We report a rare case of appendicitis caused by Streptococcus pneumoniae as the only causative organism. The case assumes significance because it occurs in the absence of any predisposing factor for invasive pneumococcal infection, it is unimicrobial, it may lead...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Bhattacharya S,Kanungo R,Natarajan MK,Mahalakshmi VN,Srinivasan K

    更新日期:2001-04-01 00:00:00

  • A case of tuberculous pericardial effusion.

    abstract::Tuberculosis accounts for up to 4% of acute pericarditis and 7% cases of cardiac tamponade. Prompt treatment can be life saving but requires accurate diagnosis. We report a case of 30-year-old male who presented with fever, chills, and dry nonproductive cough since one month. The case was diagnosed by radiological fin...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Wanjari K,Baradkar V,Mathur M,Kumar S

    更新日期:2009-01-01 00:00:00

  • Eumycetoma pedis due to Exophiala jeanselmei.

    abstract::A case of eumycetoma of foot in an 8-year old male child was clinically diagnosed as chronic osteomyelitis and was microbiologically confirmed as eumycetoma. The case is being reported for its uncommon clinical presentation and etiological agent, Exophiala jeanselmei. The patient recovered completely after treatment w...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.32726

    authors: Capoor MR,Khanna G,Nair D,Hasan A,Rajni,Deb M,Aggarwal P

    更新日期:2007-04-01 00:00:00

  • Comparison of nested polymerase chain reaction and real-time polymerase chain reaction targeting 47kda gene for the diagnosis of scrub typhus.

    abstract:Introduction:Scrub typhus is a zoonotic infection caused by Orientia tsutsugamushi which is transmitted by Leptotrombidium mites. The disease manifests as a mild-to-severe illness with non-specific clinical symptoms. Rapid diagnosis and prompt treatment are essential for patient management. Both serological and molecul...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_19_170

    authors: Srinivasan S,Kalaimani S,Jude Prakash JA,Menon T

    更新日期:2019-01-01 00:00:00

  • Staphylococcus aureus colonisation in HIV-infected patients: Incidence, risk factors and subsequent skin- and soft-tissue infections.

    abstract::We evaluated the incidence and risk factors of Staphylococcus aureus colonisation in 300 treatment-naïve HIV patients. Swabs from anterior nares and pharynx were cultured. Eighty-eight patients (29.3%) were colonised with S. aureus (47.7% nasal, 23.8% pharyngeal and 28.5% at both sites), which yielded 112 isolates. Me...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_20_5

    authors: Panigrahy A,Sinha S,Das BK,Kapil A,Vishnubhatla S,Dhawan B

    更新日期:2020-07-01 00:00:00

  • Evaluation of an indigenous Western Blot kit for human immunodeficiency virus.

    abstract:PURPOSE:The Western Blot test is considered a gold standard test for the confirmation of an ELISA and/or rapid assay screened reactive sample in the diagnosis of HIV infection, especially in the low risk population. In this study, an indigenously developed HIV W. Blot kit (J.Mitra & Co., New Delhi, India) was compared ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Lakshmi V,Ponamgi SP

    更新日期:2002-10-01 00:00:00

  • Serological diagnosis of dengue in laboratory practice in Kolkata.

    abstract:PURPOSE:To find out the most suitable serological investigative procedures to diagnose dengue cases effectively in the laboratory practice identifying primary and secondary cases as well as period of suffering. MATERIALS AND METHODS:Dengue suspected cases sent to the laboratory in 2012 in central Kolkata by the local ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.136563

    authors: Bhattacharya N,Mukherjee H,Naskar R,Talukdar S,Das G,Pramanik N,Hati AK

    更新日期:2014-07-01 00:00:00

  • Subtenons infection by Dirofilaria repens.

    abstract::Zoonotic filariasis caused by Dirofilaria repens, a parasite of carnivores occurs mainly in countries surrounding Mediterranean region. The infection occurs in and around eye among animal handlers through vector transmission. We are reporting a case of human Dirofilariasis affecting subtenons region in a 63 year-old w...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.19899

    authors: Sathyan P,Manikandan P,Bhaskar M,Padma S,Singh G,Appalaraju B

    更新日期:2006-01-01 00:00:00

  • Cutaneous cryptococcosis among HIV infected patients.

    abstract::Five patients with HIV associated cutaneous cryptococcal infection are reported. ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Vasanthi S,Padmavathy BK,Gopal R,Sundaram RS,Manoharan G

    更新日期:2002-07-01 00:00:00