A glue-based, screw-free method for implantation of intra-cranial electrodes in young mice.

Abstract:

:Intra-cranial electroencephalographic recordings are increasingly employed in mice because of the availability of genetically manipulated mouse models. Currently, dental acrylic and anchoring screws are used to cement implanted electrodes. This technique works well for adult animals but often encounters difficulty when employed in young mice because their skulls are not strong enough to bear the anchoring screws. Here we describe a novel method favorable for implantation of intra-cranial electrodes in mice as young as postnatal 18 days and suitable for long-term intra-cranial electroencephalographic recordings. Our approach is to construct a multi-electrode assembly according to the desired stereotaxic coordinates of intra-cranial recordings and to secure the implanted electrode assembly to the skull via glue rather than dental acrylic/anchoring screws. The surgical operation for such electrode implantation is relatively quick and rarely associated with complications such as infection, bleeding, neurological deficits, spontaneous seizures or behavioral disturbances. The implanted electrodes are stable, allowing repeated monitoring for several months. Data obtained by simultaneous intra-hippocampal and intra-cortical recordings indicate that our method is suitable for the examination of behaviorally related electroencephalographic activities and experimentally induced seizures. Technical aspects of our methods are discussed, and the procedures for constructing the electrode assembly are presented in detail.

journal_name

J Neurosci Methods

authors

Wu C,Wais M,Sheppy E,del Campo M,Zhang L

doi

10.1016/j.jneumeth.2008.03.001

subject

Has Abstract

pub_date

2008-06-15 00:00:00

pages

126-31

issue

1

eissn

0165-0270

issn

1872-678X

pii

S0165-0270(08)00164-7

journal_volume

171

pub_type

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